3D Nanostructured Palladium-Functionalized Graphene-Aerogel-Supported Fe 3 O 4 for Enhanced Ru(bpy) 3 2+ -Based Electrochemiluminescent Immunosensing of Prostate Specific Antigen.

We developed a novel Ru(bpy)3 2+ -based electrochemiluminescence (ECL) immunosensor utilizing palladium nanoparticle (Pd NP)-functionalized graphene-aerogel-supported Fe3 O4 (FGA-Pd) for real-sample analysis of prostate specific antigen (PSA). 3D nanostructured FGA-Pd, as a novel ECL carrier, was prepared by in situ reduction. Large amounts of Ru(bpy)3 2+ could combine with FGA-Pd via electrostatic interaction to establish a brand-new ECL emitter (Ru@FGA-Pd) for improving ECL efficiency. The obtained Ru@FGA-Pd composite was utilized to label the secondary antibody, which generated strong ECL signals with tripropylamine (TPrA) as a coreactant. Furthermore, we demonstrated that the participation of Pd NPs endowed FGA with favorable electrocatalytic ability in the luminescence process to produce more excited state [Ru(bpy)3 2+ ]* for realizing desirable signal amplification. In addition, the primary antibody was captured by gold nanoparticle (Au NP)-functionalized Fe2 O3 nanodendrites (Au-FONDs), which possessed good electrical conductivity and favorable biocompatibility. Under optimum conditions, the fabricated sandwich-type ECL immunosensor showed a sensitive response to PSA with a low detection limit of 0.056 pg/mL (S/N = 3) and a calibration range of 0.0001-50 ng/mL. Featuring favorable selectivity, stability, and repeatability, the proposed immunosensor is expected to blaze a novel trail for the real sample detection of PSA and other biomarkers.