Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout (Lep(ΔI14/ΔI14) ) rats.

Leptin deficiency is principally linked to metabolic disorders. Leptin knockout (Lep(ΔI14/ΔI14)) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders. We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue, liver and hypothalamus in Lep(ΔI14/ΔI14) and wild-type (WT) rats. We found 1,651 differentially expressed (enriched) genes in white adipose tissue, 916 in the liver, and 306 in the hypothalamus in the Lep(ΔI14/ΔI14) rats compared to WT. Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories, including fatty acid metabolism, molecular transducers and cellular processes. The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes. These data will contribute to a greater understanding of different metabolic disorders, such as obesity and diabetes.