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Quick, sensitive serial NMR experiments with Radon transform.
Journal of Magnetic Resonance 2017 July 32
The Radon transform is a potentially powerful tool for processing the data from serial spectroscopic experiments. It makes it possible to decode the rate at which frequencies of spectral peaks shift under the effect of changing conditions, such as temperature, pH, or solvent. In this paper we show how it also improves speed and sensitivity, especially in multidimensional experiments. This is particularly important in the case of low-sensitivity techniques, such as NMR spectroscopy. As an example, we demonstrate how Radon transform processing allows serial measurements of (15)N-HSQC spectra of unlabelled peptides that would otherwise be infeasible.
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