Polyxydroxybutyrate and polyhydroxydodecanoate produced by Burkholderia contaminans IPT553 PHB and PHDd produced by Burkholderia.

AIMS: In this paper, we introduce a new Burkholderia contaminans capable of producing a newly characterized polymer METHODS AND RESULTS: CG-MS and Magnetic Nuclear Resonance (1) H and (13) C were used to determine the constitution of polymers produced on glucose, glucose with casein, sucrose, and sucrose with casein. Three pairs of primers were used to find the PHA synthase class and sequence. The synthesized polymers were composed by short chain length-PHA (scl-PHA), especially polyhydroxybutyrate (PHB), and medium chain length-PHAs (mcl-PHA), especially polyhydroxydodecanoate (PHDd) and their concentration, constitution, and molecular weight depend on carbon source used. The bacterium showed only I class synthase which could not explain the mcl-PHA production CONCLUSIONS: B. contaminans has an I class PHA synthase and produces PHB combined to PHDd when cultivated in sucrose or glucose and PHDd concentration is affected when casein is used SIGNIFICANCE AND IMPACT OF THE STUDY: Polyhydroxyalkanoates (PHA) are natural polymers produced by a wide range of bacteria. The presence of PHDd monomers confers to the polymer elastomeric properties. Previously, PHDd was only obtained when bacteria were cultivated in related carbon source. In this work, B. contaminans IPT553 produced PHB with PHDd using simple and low costs carbon sources what can make possible the cheaper production of a more flexible biopolymer with of crystallinity and elasticity different from the more common PHAs. This article is protected by copyright. All rights reserved.