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Journal of Applied Microbiology

Saminathan Mookiah, Han Ming Gan, Norhani Abdullah, Clemente Michael Vui Ling Wong, Suriya Kumari Ramiah, Hui Yin Tan, Chin Chin Sieo, Yin Wan Ho
AIMS: To evaluate the effects of condensed tannins (CTs) fractions of differing molecular weights (MWs) from a Leucaena leucocephala hybrid-Rendang (LLR) on the rumen protozoal community in vitro. METHODS AND RESULTS: The effects of unfractionated CTs (F0) and CT fractions of different MWs (F1 > F2 > F3 > F4 > F5) on protozoal population and community were evaluated in vitro using rumen microbes and ground guinea grass as the substrate. Higher-MW CT fractions F1 and F2 significantly (P < 0...
April 23, 2017: Journal of Applied Microbiology
Priya Gupta, Arpita Sarkar, Padmani Sandhu, Akshay Daware, Manash C Das, Yusuf Akhter, Surajit Bhattacharjee
AIM: Pseudomonas aeruginosa is one of the fatal biofilm forming pathogens which pose to be a problem in clinical infections, contamination of food and marine ecosystems. In this report a napthoquinone - Plumbagin has been explored for its antimicrobial (antibacterial and antibiofilm) activity against P. aeruginosa biofilm. The ability of Plumbagin to enhance the bioactivity of a known broad spectrum antibiotic was further assayed by combining the sub-MIC doses of Plumbagin with sub-MIC doses of Gentamicin against P...
April 21, 2017: Journal of Applied Microbiology
Lovelia L Mamuad, Seon Ho Kim, Yeon Jae Choi, Alvin P Soriano, Kwang Keun Cho, Kichoon Lee, Gui Seck Bae, Sang Suk Lee
AIMS: This study was conducted to isolate and identify propionate-producing bacteria that can be used as an inoculum in improving wet brewers grains and rumen fermentation via increasing propionate concentration. METHODS AND RESULTS: A strain of Lactobacillus that exhibits high levels of propionate production was identified and characterized as Lactobacillus mucosae 521129 by 16S rRNA gene sequencing and phylogenetic analyses. Wet brewers grains were fermented through L...
April 20, 2017: Journal of Applied Microbiology
Jishma P, Nisma Hussain, Rakhi Chellappan, Romy Rajendran, Jyothis Mathew, Radhakrishnan E K
AIM: Analysis of blend of volatile organic compounds (VOCs) released by Pseudomonas spp. with growth promoting effect in Vigna radiata seedlings. METHODS AND RESULTS: Plant growth promoting activity of VOCs produced by five different Pseudomonas spp. was investigated by I-plate technique. VOCs produced by different organisms were extracted by various solvents (methanol, ethyl acetate, hexane and butanol) were identified by gas chromatography-mass spectrometric (GC-MS) analysis...
April 19, 2017: Journal of Applied Microbiology
Elisabeth Alice Slade, Robin Michael Statham Thorn, Andrew M Lovering, Amber Young, Darren Michael Reynolds
AIMS: To determine if bacterial species responsible for clinically relevant wound infection produce specific volatile profiles that would allow their speciation. METHODS AND RESULTS: Selected-Ion Flow Tube-Mass Spectrometry in full mass scan mode was used to analyse headspace gases produced by wound-associated bacteria grown in vitro, so as to enable identification of bacterial volatile product ion profiles in the resulting mass spectra. Applying multivariate statistical analysis (hierarchical clustering and principal component analysis) to the resultant mass spectra enabled clear speciation...
April 19, 2017: Journal of Applied Microbiology
Rebecca A Wilkes, Ludmilla Aristilde
Synthetic plastics, which are widely present in materials of everyday use, are ubiquitous and slow-degrading polymers in environmental wastes. Of special interest are the capabilities of microorganisms to accelerate their degradation. Members of the metabolically-diverse genus Pseudomonas are of particular interest due their capabilities to degrade and metabolize synthetic plastics. Pseudomonas species isolated from environmental matrices have been identified to degrade polyethylene, polypropylene, polyvinyl chloride, polystyrene, polyurethane, polyethylene terephthalate, polyethylene succinate, polyethylene glycol, and polyvinyl alcohol at varying degrees of efficiency...
April 17, 2017: Journal of Applied Microbiology
Sarang Dilip Pophaly, Poonam, Saurabh Dilip Pophaly, Suman Kapila, Dhiraj Kumar Nanda, Sudhir Kumar Tomar, Rameshwar Singh
AIMS: To assess glutathione (GSH) biosynthesis ability and activity of dependent enzymes in food grade lactic acid bacteria and correlating with genomic information on glutathione system in LAB. METHODS AND RESULTS: Whole genome sequences of twenty-six food grade LAB were screened for presence/absence of set of genes involved in de novo glutathione system. Multiple strains of S. thermophilus (37), Lb. casei (37), Lb. rhamnosus (4), Lb. paracasei (8) Lb. plantarum (23) & Lb...
April 12, 2017: Journal of Applied Microbiology
Goran M M Rashid, Maria Jesus Duran-Pena, Rahman Rahmanpour, Devin Sapsford, Timothy D H Bugg
AIMS: The aim of the study was to isolate bacterial lignin-degrading bacteria from municipal solid waste soil, and to investigate whether they could be used to delignify lignocellulose-containing soil, and enhance methane release. METHODS AND RESULTS: A set of 20 bacterial lignin degraders, including 11 new isolates from municipal solid waste soil, were tested for delignification and phenol release in soil containing 1% pine lignocellulose. A group of 7 strains were then tested for enhancement of gas release from soil containing 1% lignocellulose in small-scale column tests...
April 10, 2017: Journal of Applied Microbiology
Run-Lan Yu, A-Juan Liu, Ya-Nan Liu, Zhao-Jing Yu, Tang-Jian Peng, Xue-Ling Wu, Li Shen, Yuan-Dong Liu, Jiao-Kun Li, Xue-Duan Liu, Guan-Zhou Qiu, Miao Chen, Wei-Min Zeng
AIMS: To explore the distribution disciplinarian of alginate on the chalcopyrite concentrate surface during bioleaching. METHODS AND RESULTS: The evolution of Sulfobacillus thermosulfidooxidans secreting alginate during bioleaching of chalcopyrite concentrate was investigated through Gas Chromatograph coupled to Mass Spectrometry (GC-MS) and Confocal Laser Scanning Microscope (CLSM), and the critical synthetic genes (algA, algC, algD) of alginate were analysed by real-time polymerase chain reaction (RT-PCR)...
April 10, 2017: Journal of Applied Microbiology
Jeonghwan Jang, Hor-Gil Hur, Michael J Sadowsky, Muruleedhara N Byappanahalli, Tao Yan, Satoshi Ishii
Escherichia coli is classified as a rod-shaped, Gram-negative bacterium in the family Enterobacteriaceae. The bacterium mainly inhabits the lower intestinal tract of warm-blooded animals, including humans, and is often discharged into the environment through feces or wastewater effluent. The presence of E. coli in environmental waters has long been considered as an indicator of recent fecal pollution. However, numerous recent studies have reported that some specific strains of E. coli can survive for long periods of time, and potentially reproduce, in extra-intestinal environments...
April 6, 2017: Journal of Applied Microbiology
Fernanda Matias, Carlos Alberto Brandt, Elda Sabino da Silva, Maria Filomena de Andrade Rodrigues
In this paper, we introduce a new Burkholderia contaminans capable of producing a newly characterized polymer METHODS AND RESULTS: CG-MS and Magnetic Nuclear Resonance (1) H and (13) C were used to determine the constitution of polymers produced on glucose, glucose with casein, sucrose, and sucrose with casein. Three pairs of primers were used to find the PHA synthase class and sequence. The synthesized polymers were composed by short chain length-PHA (scl-PHA), especially polyhydroxybutyrate (PHB), and medium chain length-PHAs (mcl-PHA), especially polyhydroxydodecanoate (PHDd) and their concentration, constitution, and molecular weight depend on carbon source used...
April 6, 2017: Journal of Applied Microbiology
Samuel Collins, David Stevenson, Jimmy Walker, Allan Bennett
AIMS: To evaluate the usefulness of Legionella qPCR alongside traditional culture for enumeration of Legionella from water samples as part of both routine and public health investigation testing. METHODS AND RESULTS: Routine water samples (n=2002) and samples from public health investigations (n=215) were analysed by culture and qPCR for Legionella spp., Legionella pneumophila and L. pneumophila sg-1. A negative qPCR result was highly predictive of a negative culture result for all water systems (negative predictive values (NPV) from 97...
April 4, 2017: Journal of Applied Microbiology
M G Bonomo, C Cafaro, A Guerrieri, F Crispo, L Milella, L Calabrone, G Salzano
AIMS: This study aimed to investigate dynamics and physiological heterogeneity of Oenococcus oeni under different conditions, cell membrane fluidity and permeability variations and assessment of changes in cell surface charging rates. METHODS AND RESULTS: Flow cytometry, membrane fatty acid analysis and capillary electrophoresis were performed to study ethanol-induced variations. Different physiological states were assessed, revealing cell subpopulations able to adapt and withstand to environmental stress, in order to recover their functionality...
April 4, 2017: Journal of Applied Microbiology
Satish Kumar Verma, Kathryn Kingsley, Ivelisse Irizarry, Marshall Bergen, Ravindra Nath Kharwar, James F White
AIM: The aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. Here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens. METHODS AND RESULTS: Seed associated bacteria were removed using surface sterilization with NaOCl (bleach) followed by antibiotic treatment...
April 4, 2017: Journal of Applied Microbiology
Lucía Polo, Rosario Mañes-Lázaro, Isidoro Olmeda, Liz Erika Cruz-Pio, Ángel Medina, Sergi Ferrer, Isabel Pardo
AIMS: Determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (LAB). To know if a similar freeze-drying protocol can be used for both microorganisms. METHODS AND RESULTS: Cells from liquid culture media were recovered and concentrated in appropriate lyoprotectants. Aliquots of each strain were frozen at -20, -80 and -196°C before vacuum drying. Viable cell counts were done before freezing and after freeze-drying...
April 4, 2017: Journal of Applied Microbiology
D-L Hu, H K Ono, S Isayama, R Okada, M Okamura, L C Lei, Z S Liu, X-C Zhang, M Y Liu, J C Cui, A Nakane
AIMS: To elucidate the biological characteristics and stability of a newly identified staphylococcal enterotoxin Q (SEQ) against heating and digestive enzymes, and evaluate the risk of seq-harboring S. aureus in food poisoning. METHODS AND RESULTS: Purified SEQ was treated with heating, pepsin and trypsin that are related to food cooking, stomach and intestine conditions. Superantigenic activity of SEQ was assessed by determining the ability of IL-2 induction in mouse spleen cells...
April 4, 2017: Journal of Applied Microbiology
Zhao Chen, Xiuping Jiang
AIMS: The thermal resistance of desiccation-adapted Salmonella Senftenberg 775/W was compared with those of indigenous enterococci and total aerobic bacteria in poultry litter. METHODS AND RESULTS: Aged broiler litter and composted turkey litter with 20, 30, 40, and 50% moisture contents were inoculated with desiccation-adapted S. Senftenberg 775/W, and then heat-treated at 75 and 85°C. Compared to total aerobic bacteria, there were better correlations between mean log reductions of desiccation-adapted S...
April 3, 2017: Journal of Applied Microbiology
Mediha Yildirim-Aksoy, Benjamin H Beck
AIM: The antibacterial activities of chitosan (CS) and its derivative chitosan oligosaccharide lactate (COL) were evaluated against Aeromonas hydrophila, Edwardsiella ictaluri and Flavobacterium columnare, three highly pathogenic bacteria of warmwater finfish. METHODS AND RESULTS: The magnitude and mode of antimicrobial action on gram negative bacterial pathogens was investigated with an emphasis on examining the inhibition of bacterial growth and the weakening of barrier functions...
April 2, 2017: Journal of Applied Microbiology
Nora Jean Nealon, Colette R Worcester, Elizabeth P Ryan
AIMS: This study aimed to determine the effect of a cell-free supernatant of Lactobacillus paracasei ATCC27092 with and without rice bran extract (RBE) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. METHODS AND RESULTS: Supernatant was collected from overnight cultures of L. paracasei incubated in the presence (LP+RBE) or absence (LP) of RBE and applied to S. Typhimurium. LP+RBE reduced 13.1% more S. Typhimurium growth than LP after 16 h (P<0...
March 29, 2017: Journal of Applied Microbiology
Yousef Azarakhsh, Fatemeh Mohammadipanah, Seyed Mahdi Nassiri, Vahid Siavashi, Javad Hamedi
AIMS: Angiogenesis is a physiological process that has important impacts on pathology and healing of various diseases and its induction or inhibition by bioactive actinobacterial metabolites can help the treatment of some diseases. In this study the effects of actinobacterial extract in the process of angiogenesis have been explored. METHODS AND RESULTS: In this research, pro-angiogenic and anti-angiogenic metabolites producing actinobacteria were isolated from soil samples and their fermentation broth were extracted and after evaluation of their toxicity by MTT assay, anti-angiogenic and pro-angiogenic activities were screened against human umbilical vein endothelial cells (HUVECs) by in vitro tube formation and migration assay...
March 29, 2017: Journal of Applied Microbiology
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