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Journal of Applied Microbiology

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https://www.readbyqxmd.com/read/29144601/anti-bacterial-anti-biofilm-and-anti-quorum-sensing-effects-of-thymus-daenensis-and-satureja-hortensis-essential-oils-against-staphylococcus-aureus-isolates
#1
Aram Sharifi, Abdolmajid Mohammadzadeh, Taghi Zahraei Salehi, Pezhman Mahmoodi
AIMS: The present study was conducted to investigate the effects of Thymus daenensis and Satureja hortensis essential oils (EOs) on the planktonic growth, biofilm formation and quorum sensing (QS) of some Staphylococcus aureus isolates (strong biofilm producers). METHODS AND RESULTS: MIC of the EOs, inhibition of biofilm formation as well as disruption of preformed S. aureus biofilms were assessed. The anti-biofilm activity of the EOs was determined using microtiter-plate test (MtP) and scanning electron microscope (SEM)...
November 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29144595/evaluation-of-405-nm-monochromatic-light-for-inactivation-of-tulane-virus-on-blueberry-surfaces
#2
David Kingsley, Rafael E Perez-Perez, Glenn Boyd, Joseph Sites, Brendan A Niemira
AIM: The study aim was to evaluate the potential of 405 nm light as a virus intervention for blueberries. METHODS AND RESULTS: Tulane virus-inoculated-blueberries were treated with 4.2 mW/sq cm of 405 nm light for 5 to 30 min. To mitigate thermal heating due to the intense light, a dry ice-chilled nitrogen-based cooling system was utilized. Blueberries were rotated to ensure exposure of all surfaces to 405 light. Five, 10, and 30 min treatments resulted in little or no inactivation of Tulane virus on blueberries (average log reductions of -0...
November 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29140598/disinfection-of-chicken-fillets-in-packages-with-atmospheric-cold-plasma-effects-of-treatment-voltage-and-time
#3
Jiamei Wang, Hong Zhuang, Kurt Lawrence, Jianhao Zhang
AIMS: To study effects of treatment voltage and time of in-package atmospheric cold plasmas (ACP) on quality of raw chicken meat. METHODS AND RESULTS: Meat was packed in trays in air, treated with ACP, and stored at 4°C for 24 h or 3 d. Increasing voltage from 55 to 80 kV caused increasing O3 inside packages, but had no effects on microbes, color, and pH after 24-h storage at 4°C. There were no differences in O3 , microbes, color lightness, and pH between treatment times 3, 6 and 9 min at 80 kV after 3-d storage...
November 15, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29130635/prospective-role-of-indigenous-exiguobacterium-profundum-pt2-in-arsenic-biotransformation-and-biosorption-by-planktonic-cultures-and-biofilms
#4
Saba Saba, Rasmus Andreasen, Yinli Li, Yasir Rehman, Mehboob Ahmed, Rikke Louise Meyer, Anjum Nasim Sabri
AIMS: The aim of the present study was to analyze As transformation and biosorption by indigenous As resistant bacteria both in planktonic and biofilm modes of growth. METHODS AND RESULTS: As resistant bacteria were isolated from industrial waste water and strain PT2, identified as Exiguobacterium profundum through 16S rRNA gene sequencing was selected for further study. As transformation and biosorption by E. profundum PT2 was determined by HPLC-ICP-MS analysis...
November 12, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29130606/oral-administration-of-lactobacillus-plantarum-cjlp133-and-cjlp243-alleviates-birch-pollen-induced-allergic-rhinitis-in-mice
#5
S-P Choi, H-N Oh, C-Y Choi, H Ahn, H S Yun, Y M Chung, B Kim, S J Lee, T Chun
AIMS: In this study, we evaluated the therapeutic efficacy of selected probiotics in a mouse model of birch pollen (BP)-induced allergic rhinitis. METHODS AND RESULTS: Oral administration of lactobacillus plantarum CJLP133 and CJLP243 ameliorated the symptoms of BP-induced allergic rhinitis by reducing airway hyperresponsiveness (AHR), and both the histological scores and the number of infiltrated cells in the nasal cavities and lungs. Compared with those from vehicle-treated mice, bronchoalveolar lavage (BAL) fluid and draining lymph node samples from CJLP133 and CJLP243-administrated mice showed diminished numbers of immune cells, increased secretion of a Th1-type cytokine (IFN-γ), and decreased production of Th2-type cytokines (IL-4, IL-5 and IL-13)...
November 12, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29119696/antibacterial-isoeugenol-coating-on-stainless-steel-and-polyethylene-surfaces-prevents-biofilm-growth
#6
Christina Krogsgård Nielsen, Guruprakash Subbiahdoss, Guanghong Zeng, Zakaria Salmi, Jørgen Kjems, Tina Mygind, Torben Snabe, Rikke Louise Meyer
AIMS: Pathogenic bacteria can spread between individuals or between food items via the surfaces they share. Limiting the survival of pathogens on surfaces therefore presents an opportunity to limit at least one route of how pathogens spread. In this study we propose that a simple coating with the essential oil isoeugenol can be used to circumvent the problem of bacterial transfer via surfaces. METHODS AND RESULTS: Two commonly used materials, stainless steel and polyethylene, were coated by physical adsorption, and the coatings were characterized by Raman spectroscopy, atomic force microscopy and water contact angle measurements...
November 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29117646/lipids-from-yeasts-and-fungi-physiology-production-and-analytical-considerations
#7
REVIEW
Maria Athenaki, Chryssavgi Gardeli, Panagiota Diamantopoulou, Sidoine Sadjeu Tchakouteu, Dimitris Sarris, Antonios Philippoussis, Seraphim Papanikolaou
The last years there has been a significant rise in the number of publications in the international literature that deal with the production lipids by microbial sources (the "single cell oils; SCOs" that are produced by the so-called "oleaginous" microorganisms). In the first part of the present review-article, a general overview of the oleaginous microorganisms (mostly yeasts, algae and fungi) and their potential upon the production of SCOs is presented. Thereafter, physiological and kinetic events related with the production of, mostly, yeast and fungal lipids when sugars and related substrates like polysaccharides, glycerol, etc (the "de novo" lipid accumulation process) or hydrophobic substrates like oils and fats (the "ex novo" lipid accumulation process) were employed as microbial carbon sources, are presented and critically discussed...
November 8, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29112795/bio-analytical-applications-of-microbial-fuel-cell-based-biosensors-for-onsite-water-quality-monitoring
#8
REVIEW
Ahmed ElMekawy, Hanaa M Hegab, Deepak Pant, Christopher P Saint
Globally, sustainable provision of high quality safe water is a major challenge of the 21(st) century. Various chemical and biological monitoring analytics are presently utilized to guarantee the availability of high quality water. However, these techniques still face some challenges including high costs, complex design and onsite and online limitations. The recent technology of utilising microbial fuel cell (MFC)-based biosensors holds outstanding potential for the rapid and real-time monitoring of water source quality...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29112793/effect-of-partially-replacing-a-barley-based-concentrate-with-flaxseed-based-products-on-the-rumen-bacterial-population-of-lactating-holstein-dairy-cows
#9
E Castillo-Lopez, J Moats, N D Aluthge, H A Ramirez Ramirez, D A Christensen, T Mutsvangwa, G B Penner, S C Fernando
AIMS: The effects of partial replacement of a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows were evaluated. METHODS AND RESULTS: Treatments fed were CONT, a normal diet that included barley silage, alfalfa hay, and a barley-based concentrate that contained no flaxseed or faba beans; FLAX, inclusion of a non-extruded flaxseed-based product containing 55.0% flaxseed, 37.8% field peas and 6...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29112792/biochanin-a-improves-fiber-fermentation-by-cellulolytic-bacteria
#10
Brittany E Harlow, Michael D Flythe, Glen E Aiken
AIMS: The objective was to determine the effect of the isoflavone biochanin A (BCA) on rumen cellulolytic bacteria and consequent fermentative activity. METHODS AND RESULTS: When bovine microbial rumen cell suspensions (n = 3) were incubated (24 h, 39 °C) with ground hay, cellulolytic bacteria proliferated, short chain fatty acids were produced and pH declined. Biochanin A (30 μg mL(-1) ) had no effect on the number of cellulolyic bacteria or pH, but increased acetate, propionate and total SCFA production...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29112318/antimicrobial-activity-of-broccoli-brassica-oleracea-var-italica-cultivar-avenger-against-pathogenic-bacteria-phytopathogenic-filamentous-fungi-and-yeast
#11
R D Pacheco-Cano, R Salcedo-Hernández, J E López-Meza, D K Bideshi, J E Barboza-Corona
AIMS: The objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. METHODS AND RESULTS: Crude extracts were obtained from florets and stems of broccoli cultivar Avenger and the inhibitory effect was demonstrated against pathogenic bacteria (Bacillus cereus, Staphylococcus xylosus, S...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29112316/a-modified-dose-response-model-that-describes-the-relationship-between-hemagglutination-inhibition-titer-and-protection-against-influenza-infection
#12
Yin Huang, Steven A Anderson, Richard A Forshee, Hong Yang
AIMS: Develop a model for quantifying the risk of an adverse human response to influenza virus infection as a function of exposure dose and pre-exposure antibody titer level. METHODS AND RESULTS: We evaluated the relationship between hemagglutination inhibition (HI) titer (as a measure of specific antibody response) and protection against influenza infection and modeled this relationship by incorporating HI titer as a variable into dose-response models. Using a Maximum Likelihood Estimation approach, the resulting model was capable of providing statistically acceptable fits to most available data...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29108108/antibacterial-aryl-crowned-polyketide-from-bacillus-subtilis-associated-with-seaweed-anthophycus-longifolius
#13
Kajal Chakraborty, Bini Thilakan, Vinaya Kizhakkepatt Kizhakkekalam
AIMS: Microbiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. METHODS AND RESULTS: A culture-dependent method was used to isolate heterotrophic B. subtilis associated with A. longifolius, and assessed for its antimicrobial properties...
November 6, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29095556/antibacterial-and-antifungal-activity-activity-of-pyroligneous-acid-from-wood-of-eucalyptus-urograndis-and-mimosa-tenuiflora
#14
Eduardo de Souza Araújo, Alexandre Santos Pimenta, Francisco M C Feijó, Renato Vinicius Oliveira Castro, Maíra Fasciotti, Thays V C Monteiro, Kássio Michel Gomes de Lima
AIMS: The present work aimed to evaluate the antibacterial and antifungal activity of two types of pyroligneous acid (PA) obtained from slow pyrolysis of wood of Mimosa tenuiflora and of a hybrid of Eucalyptus urophylla x Eucalyptus grandis. METHODS AND RESULTS: Wood wedges were carbonized on a heating rate of 1.25°C/min until 450°C. Pyrolysis smoke was trapped and condensed to yield liquid products. Crude pyrolysis liquids were bi-distilled under 5 mmHg vacuum yielding purified PA...
November 2, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29094428/an-overview-of-20-years-of-studies-on-the-prevalence-of-human-enteric-viruses-in-shellfish-from-galicia-spain
#15
Jesús L Romalde, Enrique Rivadulla, Miguel F Varela, Juan L Barja
Galicia (NW Spain) has 1,490 km of coastline, and its particular topography, characterized by the presence of fiord-like inlets, called rías, with an important primary production, make this region very favourable for shellfish growth and culture. In fact, Galicia is one of the most important mussel producers in the world. Due to the proximity to cities and villages, and the anthropogenic activities in these estuaries, and despite the routinely official controls of the bivalve harvesting areas, contamination with material of faecal origin is sometimes possible but, whereas current regulation based on Escherichia coli as indicator microorganism has been revealed as useful for bacterial contaminants, this is not the case for enteric viruses...
November 2, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29091336/evaluation-of-the-use-of-malic-acid-decarboxylase-deficient-starter-culture-in-nacl-free-cucumber-fermentations-to-reduce-bloating-incidence
#16
Y Zhai, I M Pérez-Díaz, J T Diaz, R L Lombardi, L Connelly
AIMS: Accumulation of carbon dioxide (CO2 ) in cucumber fermentations is known to cause hollow cavities inside whole fruits or bloaters, conducive to economic losses for the pickling industry. This study focused on evaluating the use of a malic acid decarboxylase (MDC) deficient starter culture to minimize CO2 production and the resulting bloater index in NaCl free cucumber fermentations brined with CaCl2 . METHODS AND RESULTS: Attempts to isolate autochthonous MDC deficient starter cultures from commercial fermentations, using the MD medium for screening, were unsuccessful...
November 1, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29080295/effect-of-riboflavin-producing-bacteria-against-chemically-induced-colitis-in-mice
#17
Romina Levit, Graciela Savoy de Giori, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc
AIM: To assess the anti-inflammatory effect associated to individual probiotic suspensions of riboflavin-producing lactic acid bacteria (LAB) in a colitis murine model. METHODS AND RESULTS: Mice intrarectally inoculated with trinitrobenzene sulfonic acid (TNBS) were orally administered with individual suspensions of riboflavin-producing strains: Lactobacillus (Lact.) plantarum CRL2130, Lact. paracasei CRL76, Lact. bulgaricus CRL871, Streptococcus thermophilus CRL803; a non riboflavin-producing strain or commercial riboflavin...
October 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29080234/an-improved-model-for-the-analysis-of-combined-antimicrobials-a-replacement-for-the-chou-talalay-combination-index-method
#18
Maria Anastasiadi, Karen Polizzi, Ronald J W Lambert
AIMS: To rationalise confusion in the literature concerning the analysis of combined antimicrobials, specifically to see if the combination index (CI) method of analysis was as rigorous as claimed. METHODS & RESULTS: data from previous studies of the inhibition of Staphylococcus aureus by mixed antimicrobials were re-analysed using the CI method and a model which takes account of differences in the concentration exponents of individual antimicrobials. CONCLUSIONS: The Chou-Talalay combination index method for the analysis of combined antimicrobials was found to be valid only in the specific cases where concentration exponents were equal...
October 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29080227/yeasts-and-moulds-contaminants-of-food-ice-cubes-and-their-survival-in-different-drinks
#19
N Francesca, R Gaglio, C Stucchi, S De Martino, G Moschetti, L Settanni
AIMS: To evaluate the levels of unicellular and filamentous fungi in ice cubes produced at different levels and to determine their survival in alcoholic beverages and soft drinks. METHODS AND RESULTS: Sixty samples of ice cubes collected from home level (HL) productions, bars and pubs (BP) and industrial manufacturing plants (MP) were investigated for the presence and cell density of yeasts and moulds. Moulds were detected in almost all samples, while yeasts developed from the majority of HL and MP samples...
October 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29078036/selection-of-cellular-genetic-markers-for-the-detection-of-infectious-poliovirus
#20
Daisuke Sano, Megumi Tazawa, Manami Inaba, Syunsuke Kadoya, Ryosuke Watanabe, Takayuki Miura, Masaaki Kitajima, Satoshi Okabe
AIMS: Cellular responses of an established cell line from human intestinal epithelial cells (INT-407 cells) against poliovirus infections were investigated in order to find cellular genetic markers for infectious poliovirus detection. METHODS AND RESULTS: Gene expression profile of INT-407 cells was analyzed by DNA microarray technique when cells were infected with poliovirus 1 (PV1) (sabin) at multiplicity of infection (MOI) of 10(-3) and incubated for 12h. Poliovirus infection significantly altered the gene expressions of two ion channels, KCNJ4 and SCN7A...
October 27, 2017: Journal of Applied Microbiology
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