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Journal of Applied Microbiology

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https://www.readbyqxmd.com/read/28321957/activity-of-exogenous-tyrosol-in-combination-with-caspofungin-and-micafungin-against-candida-parapsilosis-sessile-cells
#1
Renátó Kovács, Zoltán Tóth, Fruzsina Nagy, Lajos Daróczi, Aliz Bozó, László Majoros
AIMS: Fungal quorum sensing molecules may have an inhibitory effect as adjuvant against Candida biofilms. Therefore, in vitro activity of caspofungin and micafungin was evaluated against Candida parapsilosis biofilms in the presence of tyrosol. METHODS AND RESULTS: Interactions were assessed using fractional inhibitory concentration index (FICI) determination, metabolic activity-based time-kill experiments, and scanning electron microscopy. Tyrosol caused 1- to 16-fold and 2- to 32-fold decrease in median caspofungin and micafungin MICs, respectively...
March 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28317285/changes-in-the-relative-population-size-of-selected-ruminal-bacteria-following-an-induced-episode-of-acidosis-in-beef-heifers-receiving-viable-and-non-viable-active-dried-yeast
#2
R Mohammed, D Vyas, W Z Yang, K A Beauchemin
AIMS: To characterize the changes in the relative population size (RPS) of select ruminal bacteria and rumen fermentation variables in beef heifers supplemented with a strain of Saccharomyces cerevisiae as viable active dried (ADY) or killed dried (KDY) yeast following an induced episode of ruminal acidosis. METHODS AND RESULTS: Six ruminally-cannulated beef heifers fed a diet consisting of 50% forage and 50% grain (dry matter basis) were used in a replicated 3 × 3 Latin square design with three 28 d periods...
March 20, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28314076/lactococcus-lactis-carrying-a-dna-vaccine-coding-for-the-esat-6-antigen-increases-il-17-cytokine-secretion-and-boosts-the-bcg-vaccine-immune-response
#3
Vanessa Bastos Pereira, Vanessa Pecini da Cunha, Tatiane Melo Preisser, Bianca Mendes Souza, Meritxell Zurita Turk, Camila Prósperi De Castro, Marcela Santiago Pacheco Azevedo, Anderson Miyoshi
AIMS: A regimen utilizing BCG and another vaccine system as a booster may represent a promising strategy for the development of an efficient tuberculosis vaccine for adults. In a previous work, we confirmed the ability of Lactococcus lactis FnBPA+ (pValac:ESAT-6), a live mucosal DNA vaccine, to produce a specific immune response in mice after oral immunization. In the present study, we examined the immunogenicity of this strain as a booster for the BCG vaccine in mice. METHODS AND RESULTS: After immunization, cytokine and immunoglobulin profiles were measured...
March 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28314049/biological-evaluation-of-3-hydroxybenzyl-alcohol-an-extrolite-produced-by-aspergillus-nidulans-strain-kzr-132
#4
C Ganesh Kumar, Poornima Mongolla, Sujitha Pombala, Shiva Bandi, K Suresh Babu, Kallaganti Venkata Siva Ramakrishna
AIMS: The aim of the study was to purify and characterize a bioactive compound from Aspergillus nidulans strain KZR-132 and its biological evaluation. METHODS AND RESULTS: A bioactive extolite was purified from Aspergillus nidulans strain KZR-132, and its chemical structure was elucidated as 3-hydroxylbenzyl alcohol (3-HBA) based on (1) H and (13) C NMR, FT-IR and mass spectroscopic analysis. The antimicrobial efficacy of 3-HBA was established against Gram-positive, Gram-negative bacteria and different Candida strains...
March 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28301710/aflatoxin-contamination-of-groundnut-and-maize-in-zambia-observed-and-potential-concentrations
#5
Paul W Kachapulula, Juliet Akello, Ranajit Bandyopadhyay, Peter J Cotty
AIMS: Quantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and determine vulnerability to aflatoxin increases after purchase. METHODS AND RESULTS: Aflatoxin concentrations were determined for 334 maize and groundnut samples from 27 districts using lateral-flow immunochromatography. Seventeen percent of crops from markets contained aflatoxin concentrations above allowable levels in Zambia (10 μg kg(-1) )...
March 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28295891/molecular-serotyping-virulence-gene-profiling-and-pathogenicity-of-streptococcus-agalactiae-isolated-from-tilapia-farms-in-thailand-by-multiplex-pcr
#6
Korntip Kannika, Duangjai Pisuttharachai, Prapansak Srisapoome, Janenuj Wongtavatchai, Hidehiro Kondo, Ikuo Hirono, Sasimanas Unajak, Nontawith Areechon
AIMS: This study aimed to biotype Streptococcus agalactiae isolated from tilapia farms in Thailand based on molecular biotyping methods and to determine the correlation between the serotype and virulence of bacteria. In addition to a biotyping (serotyping) technique based on multiplex PCR of cps genes, in this study, we developed multiplex PCR typing of Group B streptococcus (GBS) virulence genes to examine 3 clusters of virulence genes and their correlation to the pathogenicity of S...
March 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28295939/lactic-acid-bacteria-promising-vaccine-vectors-possibilities-limitations-doubts
#7
REVIEW
Katarzyna Szatraj, Agnieszka K Szczepankowska, Magdalena Chmielewska-Jeznach
Gram-positive, nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of novel, safe production and delivery systems of heterologous proteins. Recombinant LAB strains were shown to elicit specific systemic and mucosal immune responses against selected antigens. For this reason this group of bacteria is considered as a potential replacement of classical, often pathogenic, attenuated bacteria carriers. Mucosal administration of recombinant LAB, especially via the best explored and universal oral route, offers many advantages in comparison to systemic inoculation, and is attractive from the immunological and practical point of view...
March 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28294483/the-detection-and-quantification-of-bacillus-thuringiensis-spores-from-soil-and-swabs-using-quantitative-pcr-as-a-model-system-for-routine-diagnostics-of-bacillus-anthracis
#8
Vera Sedlackova, Radka Dziedzinska, Vladimir Babak, Petr Kralik
AIMS: To optimize the DNA isolation for the routine detection and quantification of bacillary spores in soil and swabs. The procedure is primarily intended for diagnostics of Bacillus anthracis spores, but due to its high pathogenicity B. thuringiensis served as its surrogate organism. METHODS AND RESULTS: Various commercial kits for soils and swabs in combination with quantitative PCR were tested with different results. The PowerSoil DNA kit and the Ultra Clean Microbial DNA kit gave the best results for the extraction from soil and swabs, respectively...
March 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28294465/lactococcus-lactis-kr-050l-inhibit-il-6-stat3-activation
#9
Joo Tae Hwang, Hyun-Jae Jang, Jin Han Kim, Chan Sun Park, Yesol Kim, Chi-Hwan Lim, Seung Woong Lee, Mun-Chual Rho
AIMS: The purpose of this study is to investigate IL-6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi. METHODS AND RESULTS: Six lactic acid bacteria (LABs) were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR-050L inhibited IL-6-induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative HPLC, and they were identified as 2,5-diketopipperazine structures by spectroscopic analyses (MS, (1) H- and (13) C-NMR)...
March 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28276616/co-expression-of-sulfhydryl-oxidase-and-protein-disulfide-isomerase-in-escherichia-coli-allows-for-production-of-soluble-crm197
#10
Robyn Roth, Petrus van Zyl, Tsepo Tsekoa, Stoyan Stoychev, Sipho Mamputha, Sindisiwe Buthelezi, Michael Crampton
AIMS: To investigate the production of soluble Cross-Reacting Material 197 (CRM197 ) in Escherichia coli, a safe and effective T-cell dependent protein carrier for polysaccharides used in the manufacture and application of multivalent conjugate vaccines. METHODS AND RESULTS: The use of co-expression of a sulfhydryl oxidase and protein disulfide isomerase for the production of soluble CRM197 in E. coli is described. CRM197 contains two disulfide bonds, which are normally unable to form in the reducing environment of the E...
March 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28276612/a-gut-reaction-the-combined-influence-of-exercise-and-diet-on-gastrointestinal-microbiota-in-rats
#11
Romeo Batacan, Andrew Fenning, Vincent J Dalbo, Aaron T Scanlan, Mitch J Duncan, Robert J Moore, Dragana Stanley
AIMS: Intestinal microbiota modulates the development of clinical conditions, including metabolic syndrome and obesity. Many of these conditions are influenced by nutritional and exercise behaviours. This study aimed to investigate the ability of exercise to re-shape the intestinal microbiota and the influence of the diet on the process. METHODS AND RESULTS: A rat model was used to examine the intestinal microbiota responses to four activity conditions, including: high intensity interval training, light intensity training, sedentary and normal control, each containing two nutritional conditions: high-fat high-fructose diet (HF) and standard chow (SC) diet...
March 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28276610/the-trans-10-cis-12-conjugated-linoleic-acid-increases-triacylglycerol-hydrolysis-in-yeast-saccharomyces-cerevisiae
#12
Sanna Hokkanen, Simo Laakso, Claudia M Senn, Alexander D Frey
AIMS: The trans-10,cis-12 conjugated linoleic acid (CLA) is known for its antilipogenic effect but the mechanism is not fully clear. In this study, potential of yeast (Saccharomyces cerevisiae) metabolism to offer evidence for the mechanism was investigated. METHODS AND RESULTS: Inhibitory effect of CLA on lipid accumulation was studied by analyzing the transcript abundance of selected genes involved in TAG synthesis (LRO1, DGA1, ARE1 and ARE2) in the presence of the two bioactive CLA isomers: trans-10,cis-12 and the cis-9,trans-11 CLA...
March 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28261951/susceptibility-to-disinfectants-in-antimicrobial-resistant-and-susceptible-isolates-of-escherichia-coli-enterococcus-faecalis-and-enterococcus-faecium-from-poultry-esbl-ampc-phenotype-of-escherichia-coli-is-not-associated-with-resistance-to-a-quaternary-ammonium
#13
Nadine Wieland, Julia Boss, Sarah Lettmann, Barbara Fritz, Karin Schwaiger, Johann Bauer, Christina S Hölzel
AIMS: The spread of bacteria that are simultaneously resistant to disinfectants and antimicrobials would constitute an unsettling scenario. In order to explore an association between antimicrobial resistance and reduced susceptibility to biocides / microbicides like disinfectants in agriculture, we investigated Escherichia (E.) coli (n = 438) and enterococci (n = 120) isolated from six different flocks of the same poultry farm with known history of antimicrobial treatment. METHODS AND RESULTS: Susceptibility to disinfectants (formic acid and a quaternary ammonium compound, didecyldimethlyammoniumchloride - DDAC) was assessed by macrodilution (German Veterinary Society)...
March 6, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28261897/coproduction-of-colicin-v-and-lactic-acid-bacteria-bacteriocins-in-lactococci-and-enterococci-strains-of-biotechnological-interest
#14
S Langa, J L Arqués, M Medina, J M Landete
AIMS: The aim of this study was the coproduction in a single strain of the Gram-negative bacteriocin colicin V with other bacteriocins from lactic acid bacteria. METHODS AND RESULTS: Colicin V was expressed in Lactococcus and Enterococcus strains by replacing the colicin V leader peptide by the leader peptide and promoter of D-alanyl-D-alanine carboxypeptidase from Lactobacillus reuteri CECT925 (pNZ:LR-colV). The antimicrobial activity of colicin V against the indicator organism E...
March 6, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28258625/modelling-based-identification-of-factors-influencing-campylobacters-in-chicken-broiler-houses-and-on-carcasses-sampled-after-processing-and-chilling
#15
M L Hutchison, M J Taylor, M A Tchòrzewska, G Ford, R H Madden, T G Knowles
AIMS: To identify production and processing practices that might reduce Campylobacter numbers contaminating chicken broiler carcasses. METHODS AND RESULTS: Numbers of campylobacters were determined on carcass neck skins after processing or in broiler house litter samples. Supplementary information that described farm layouts, farming conditions for individual flocks, the slaughterhouse layouts and operating conditions inside plants was collected, matched with each Campylobacter test result...
March 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28256070/selective-method-for-identification-and-quantification-of-bifidobacterium-animalis-subspecies-lactis-bb-12-bb-12-from-the-gastrointestinal-tract-of-healthy-volunteers-ingesting-a-combination-probiotic-of-bb-12-and-lactobacillus-rhamnosus-gg-lgg
#16
D D Poutsiaka, I J Mahoney, L A McDermott, L Lathrop Stern, C M Thorpe, A V Kane, C Baez-Giangreco, J McKinney, L E Davidson, R Leyva, B Goldin, D R Snydman
AIM: To develop a novel validated method for the isolation of Bifidobacterium animalis subsp lactis BB-12 (BB-12) from fecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. METHODS AND RESULTS: A novel method for isolating and enumerating BB-12 was developed based on its morphologic features of growth on tetracycline-containing agar. The method identified BB-12 correctly from spiked stool close to100% of the time as validated by PCR confirmation of identity, and resulted in 97-104% recovery of BB-12...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28256040/applications-and-safety-considerations-of-lactobacillus-salivarius-as-a-probiotic-in-animal-and-human-health
#17
REVIEW
Byron D Chaves, Mindy M Brashears, Kendra K Nightingale
The goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal health, particularly to reduce colonization by gastrointestinal (GI) pathogens, and to a lesser extent, as a production and quality aid. In humans, L. salivarius has been used to prevent and treat a variety of chronic diseases, including asthma, cancer, atopic dermatitis, and halitosis, and to a much limited extent, to prevent or treat infections...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28256039/biotransformation-of-ginsenoside-rb1-to-ginsenoside-rg3-by-endophytic-bacterium-burkholderia-sp-ge-17-7-isolated-from-panax-ginseng
#18
Yu Fu, Zhenhao Yin, Chengri Yin
AIMS: To isolate a novel endophytic bacteriums from Panax ginseng that could have excellent properties on converting ginsenoside Rb1 to ginsenoside Rg3. METHODS AND RESULTS: Based on 16S rDNA gene sequence, the strain named GE 17-7 was identified as Burkholderia sp. This strain have showed the highest activity in convertion ginsenosides Rb1 into 20(S)-ginsenoside Rg3. During the biotransformation of ginsenoside Rb1, the final metabolite was identified by Nuclear Magnetic Resonance (NMR) analysis and the transformation pathway of ginsenoside Rb1 was also identified by Thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) analysis in this study...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28256031/microbiome-dynamic-modulation-through-functional-diets-based-on-pre-and-probiotics-mannan-oligosaccharides-and-saccharomyces-cerevisiae-in-juvenile-rainbow-trout-oncorhynchus-mykiss
#19
Ana Teresa Gonçalves, Cristian Gallardo-Escárate
AIMS: This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0.6% mannan-oligosaccharides and 0.5% Saccharomyces cerevisiae respectively) or the mixture of both. METHODS AND RESULTS: A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28251734/antimicrobial-activity-of-2-mercaptobenzothiazole-released-from-environmentally-friendly-nanostructured-layered-double-hydroxides
#20
A Kuznetsova, P M Domingues, T Silva, A Almeida, M L Zheludkevich, J Tedim, M G S Ferreira, A Cunha
AIMS: The objective of this work was to assess the antibacterial effect of 2-mercaptobenzothiazole (MBT), used as model-biocide, immobilized in a layered double hydroxide (LDH) structure, under different conditions of pH and salinity, envisaging possible applications of the system in active antifouling and anticorrosion coatings. METHODS AND RESULTS: Biological effects of MBT immobilized in LDH were assessed by monitoring bacterial bioluminescence of cell suspensions of either Allivibrio fischeri or a recombinant strain of Escherichia coli, as a proxy for bacterial activity...
March 2, 2017: Journal of Applied Microbiology
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