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Journal of Applied Microbiology

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https://www.readbyqxmd.com/read/28718996/production-of-ligninolytic-enzymes-and-some-diffusible-antifungal-compounds-by-white-rot-fungi-using-potato-solid-wastes-as-the-sole-nutrient-source
#1
H Schalchli, E Hormazábal, O Rubilar, G Briceño, A Mutis, G J Zocolo, C Diez
AIMS: The aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using peels or discarded potato as the sole nutrient source. METHODS AND RESULTS: The strain Trametes hirsuta Ru-513 has been highlighted for its laccase activity (595 ± 33 U l(-1) ), which is able to decolourise 87% of an anthraquinone dye using potato peels as the sole nutritional support. A native polyacrylamide gel of laccase proteins showed the presence of two isoenzymes, corresponding to proteins of 56 and 67 kDa, which were detected by SDS-PAGE...
July 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28718990/fungitoxicity-of-organic-extracts-of-ocimum-basilicum-on-growth-and-morphogenesis-of-bipolaris-species-teleomorph-cochliobolus
#2
E A Elsherbiny, N A Safwat, M M Elaasser
AIMS: This study aimed at evaluating the inhibitory effects of various organic extracts of Ocimum basilicum against some species of Bipolaris and Cochliobolus with GC-MS and HPLC analysis. METHODS AND RESULTS: The ethyl acetate extract consisted of methyl cinnamate as the most abundant component, while butylated hydroxytoluene was the major component in the methanol extract. Pyrogallol and chlorogenic acid were major phenolic compounds in the ethyl acetate and methanol extracts, respectively...
July 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28714250/development-of-antimicrobial-nanoemulsion-based-delivery-systems-against-selected-pathogenic-bacteria-using-a-thymol-rich-thymus-daenensis-essential-oil
#3
Lida Ghaderi, Roya Moghimi, Atousa Aliahmadi, David Julian McClements, Hasan Rafati
AIMS: Thymol rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water-solubility and high vapor pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in water by preventing Ostwald ripening and phase separation. METHODS & RESULTS: The antibacterial activity of bulk and emulsified essential oil against selected pathogenic bacteria including Gram-negative (Haemophilus influenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) were investigated in the liquid and vapor phase...
July 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28714193/alteration-of-oxidative-phosphorylation-as-a-possible-mechanism-of-the-antifungal-action-of-p-coumaric-acid-against-botrytis-cinerea
#4
J Morales, L Mendoza, M Cotoras
AIMS: The aim of this study was to analyze the mechanism of action of p-coumaric acid against isolate B05.10 of B. cinerea. For this purpose, the effect of this compound on cell membrane, cell wall and oxidative phosphorylation was determined. Induction of oxidative stress triggered by this compound was also studied. METHODS AND RESULTS: p-coumaric acid showed antifungal effect on the mycelial growth-. Additionally, the compound was able to retard the germination of B...
July 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28712149/occurrence-genetic-diversity-and-antibiotic-resistance-of-arcobacter-spp-in-a-dairy-plant
#5
Susana Ferreira, Mónica Oleastro, Fernanda C Domingues
AIMS: The aim of this study was to evaluate the occurrence, diversity and resistance to antibiotics of Arcobacter spp. in a dairy plant samples. METHODS AND RESULTS: A total of 75 samples from dairy plant surfaces and materials and several food products collected in different steps of the cheese production process were analysed by culture, under aerobic and microaerobic atmospheric conditions, and by enrichment molecular detection. Isolates were identified and genotyped by ERIC-PCR, and their susceptibility to nine antibiotics was evaluated by agar dilution...
July 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28712131/a-novel-antibacterial-peptide-derived-from-crocodylus-siamensis-hemoglobin-hydrolysate-induces-membrane-permeabilisation-causing-iron-dysregulation-oxidative-stress-and-bacterial-death
#6
J Lueangsakulthai, N Jangpromma, T Temsiripong, J E McKendrick, W Khunkitti, S E Maddocks, S Klaynongsruang
AIMS: A novel antibacterial peptide from Crocodylus siamensis hemoglobin hydrolysate (CHHs) was characterised for antimicrobial activity. METHODS AND RESULTS: CHHs was hydrolysed for 2 h (2h-CHH), 4 h (4h-CHH), 6 h (6h-CHH) and 8 h (8h-CHH). 8h-CHH showed antibacterial activity against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa at concentrations of 20, 20, 20 and 10 mg ml(-1) (w/v), respectively. Fluorescent microscopy revealed that 8h-CHH had bactericidal activity against E...
July 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28708321/dynamics-of-culturable-mesophilic-bacterial-communities-of-three-fresh-herbs-and-their-production-environment
#7
Maria-Theresia Gekenidis, Diane Gossin, Mathias Schmelcher, Ulrich Schöner, Mitja N P Remus-Emsermann, David Drissner
AIM: Investigate dynamics of culturable mesophilic bacteria and selected food contaminating bacteria from three herbs and their production environment. METHODS AND RESULTS: Marjoram, basil, and thyme were investigated during one growing season by sampling plants, organic fertilizers, soil, irrigation water, and marketed products. Mesophilic bacteria and selected food contaminating bacteria (Escherichia coli, Enterococcus spp., Bacillus cereus group) were cultured and identified by MALDI biotyping...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28708308/arsenic-resistant-and-plant-growth-promoting-firmicutes-and-%C3%AE-proteobacteria-species-from-industrially-polluted-irrigation-water-and-corresponding-cropland
#8
Nayab Qamar, Yasir Rehman, Shahida Hasnain
AIMS: The aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for As-detoxification and plant growth promotion. METHODS AND RESULTS: Plant growth promoting As-resistant cropland bacteria were isolated from contaminated irrigation water and corresponding agricultural soil. Phylogenetic analysis revealed that the isolates belonged to 2 distinct bacterial lineages; Firmicutes and γ-Proteobacteria...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28708293/investigation-on-the-antimicrobial-activities-of-gingers-etlingera-coccinea-blume-s-sakai-nagam-and-etlingera-sessilanthera-r-m-sm-endemic-to-borneo
#9
Delhousie Daniel-Jambun, Jacky Dwiyanto, Yau Yan Lim, Joash Ban Lee Tan, Azira Muhamad, Sau Wai Yap, Sui Mae Lee
AIMS: To investigate the antimicrobial properties of E. coccinea and E. sessilanthera and to isolate and identify the antimicrobial compounds. METHODS AND RESULTS: Extracts were obtained via sequential solvent extraction method using hexane, dichloromethane, ethyl acetate, methanol and water. Antimicrobial activity testing was done using broth microdilution assay against 17 strains of bacteria. The leaf hexane extract of E. coccinea and rhizome hexane extract of E...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28708280/biodegradation-of-ochratoxin-a-by-alcaligenes-faecalis-isolated-from-soil
#10
H H Zhang, Y Wang, C Zhao, J Wang, X L Zhang
AIMS: The aim of the present work is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A. METHODS AND RESULTS: An Alcaligenes faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results show that this strain could degrade ochratoxin A efficiently but could not use it as a sole carbon source. Ochratoxin α was confirmed as a degradation product in the intracellular extract of Alcaligenes faecalis using UPLC-MS/MS...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28688204/enhancing-expression-of-the-pseudorabies-virus-glycoprotein-e-in-yeast-and-its-application-in-an-indirect-sandwich-elisa
#11
Ching-Ying Wu, Ching-Wei Wu, Chih-Ming Liao, Maw-Sheng Chien, Chienjin Huang
AIMS: The purpose of this study was to produce a recombinant pseudorabies virus (PRV) glycoprotein E (gE) protein with the correct antigenicity for use as a low-cost diagnostic antigen. METHODS AND RESULTS: The gene fragment encoding the amino-terminal immunodominant region of PRV gE (codons 31-270) (gEN31-270) was codon-optimized and expressed constitutively and secreted using a Pichia pastoris expression system. Yeast-expressed gEN31-270 (ygEN31-270) was harvested from the culture supernatant, and ygEN31-270 was shown to exhibit N-linked glycosylation...
July 8, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28667801/a-study-of-selected-factors-affecting-efficacy-of-compost-tea-against-several-fungal-pathogens-of-potato
#12
W K Mengesha, W M Gill, S M Powell, K J Evans, K M Barry
AIM: To investigate selected factors of two non-aerated compost teas and mechanisms that influence the restriction of several fungal potato pathogens. METHODS AND RESULTS: Two non-aerated compost teas (NCTs, commercial compost, CCT; vineyard compost, VCT) were tested for their ability to suppress potato pathogens. The VCT was more suppressive than CCT to mycelial growth of A. solani and R. solani isolate 299, but not for R. solani isolate 422. Metagenomic studies of microbial diversity revealed that the CCT had higher fungal and bacterial diversity and richness than the VCT...
July 1, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28667709/relationship-between-expression-level-of-hygromycin-b-resistant-gene-and-agrobacterium-tumefaciens-mediated-transformation-efficiency-in-beauveria-bassiana-jef-007
#13
Y S Nai, M R Lee, S Kim, S J Lee, J C Kim, Y T Yang, J S Kim
AIMS: Agrobacterium tumefaciens-mediated transformation (AtMT) is an effective method for generation of entomopathogenic Beauveria bassiana transformants. However still antibiotics-susceptible strain shows slow growth as a background in the selective antibiotics (hygromycin B; HygB) medium, which reduces the selection efficiency of the putative transformants. Herein this work, a relationship between HygB resistance gene promoter and AtMT efficiency was investigated to improve the transformant selection...
July 1, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28654203/effects-of-storage-temperature-on-bacterial-growth-rates-and-community-structure-in-fresh-retail-sushi
#14
Sunniva Hoel, Anita Nordeng Jakobsen, Olav Vadstein
AIMS: This study was conducted to assess the effects of different storage temperatures (4 to 20 °C), on bacterial concentrations, growth rates and community structure in fresh retail sushi, a popular retail product with a claimed shelf life of two to three days. METHODS AND RESULTS: The maximum specific growth rate based on aerobic plate count (APC) at 4 °C was 0.06 h(-1) , and displayed a 6-fold increase (0.37 h(-1) ) at 20 °C. Refrigeration resulted in no growth of hydrogen sulfide (H2 S)-producing bacteria, but this group had the strongest temperature response...
June 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650591/the-silver-lining-towards-the-responsible-and-limited-usage-of-silver
#15
REVIEW
Kshipra Naik, Meenal Kowshik
Silver has attracted a lot of attention as a powerful, broad spectrum and natural antimicrobial agent since the ancient times because of its non-toxic nature to the human body at low concentrations. It has been used in treatment of various infections and ulcers, storage of water, and prevention of bacterial growth on the surfaces and within materials. However there are numerous medical and health benefits of colloidal or nano silver apart from its microbicidal ability which as yet have not been fully embraced by the medical community...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650559/the-antibacterial-activity-and-modes-of-li-f-type-antimicrobial-peptides-against-bacillus-cereus-in-vitro
#16
Jinzhi Han, Shengming Zhao, Zhi Ma, Ling Gao, Hongxia Liu, Umair Muhammad, Zhaoxin Lu, Fengxia Lv, Xiaomei Bie
AIMS: LI-Fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (Gram-positive bacteria and fungi). In this study, LI-F type antimicrobial peptides (AMP-jsa9) composing of LI-F03a, LI-F03b, LI-F04a, LI-F04b, and LI-F05b were isolated from Paenibacillus polymyxa JSA-9. To better understand the antimicrobial mechanism of AMP-jsa9, the potency and action(s) of AMP-jsa9 against B. cereus were examined. METHODS AND RESULTS: Flow cytometry, confocal laser microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM) observation, as well as determination of peptidoglycan and cell wall associated protein, and other methods were used...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650523/characterization-of-the-phosphate-specific-transport-system-in-cronobacter-sakazakii-baa-894
#17
Xiujun Liang, Xiaoqing Hu, Xiaoyuan Wang, Jianli Wang, Yu Fang, Yanyan Li
AIMS: Characterize the phosphate-specific transport system in Cronobacter sakazakii BAA-894. METHODS AND RESULTS: The genes relevant to phosphate transfer in C. sakazakii BAA-894 were determined by using sequence alignment to the corresponding genes in Escherichia coli. Then, the determined pst operon in C. sakazakii BAA-894 was deleted using the lambda Red recombination system. Using the wild type C. sakazakii BAA-894 as a control, the membrane permeability, auto-aggregation, exopolysaccharide biosynthesis, biofilm formation, and adhesion ability of the mutant △pst grown in media containing high or low concentrations of phosphate were investigated; stronger auto-aggregation, less biofilm formation and higher adhesion ability were observed in △pst cells grown in low phosphate media...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650129/in-vitro-efficacy-and-in-silico-analysis-of-cefixime-ofloxacin-combination-for-salmonella-typhi-from-bloodstream-infection
#18
Yamuna Devi Bakthavatchalam, Thirumal Kumar D, Iftikhar Aslam Tayubi, Baby Abirami S, Priyanka Babu, Elakkiya Munusamy, Bhuvaneswari Thukkaram, Raji Ravi, George Priya Doss C, Balaji Veeraraghavan
AIMS: Recently, the cefixime-ofloxacin combination is approved by drug controller general of India (DCGI) to treat typhoid fever. We sought to evaluate the antimicrobial activity of cefixime-ofloxacin combination against S. Typhi. METHODS AND RESULTS: 283 non-duplicate S. Typhi isolates collected during 2012 to 2014 were included in this study. Minimum inhibitory concentration (MIC) of cefixime and ofloxacin was determined by using broth microdilution method. Combinational testing was performed by using checkerboard assay...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28649706/improving-efficiency-of-viability-qpcr-for-selective-detection-of-infectious-hav-in-food-and-water-samples
#19
W Randazzo, Joaquín Piqueras, Jesús Rodríguez-Díaz, R Aznar, G Sánchez
AIM: To improve the efficacy of intercalating dyes to distinguishing between infectious and inactivated hepatitis A virus (HAV) in food. METHODS AND RESULTS: Different intercalating dyes were evaluated for the discrimination between infectious and thermally-inactivated HAV suspensions combining with the RT-qPCR proposed in the ISO 15216. Among them, PMAxx was the best dye in removing the RT-qPCR signal from inactivated HAV. Applied to lettuce and spinach, PMAxx-Triton pretreatment resulted in complete removal of the RT-qPCR signal from inactivated HAV...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28644912/biofilm-formation-and-disinfectant-resistance-of-salmonella-spp-in-mono-and-dual-species-with-pseudomonas-aeruginosa
#20
Xinyi Pang, Yishan Yang, Hyun-Gyun Yuk
AIMS: This study aimed to evaluate the biofilm formation and disinfectant resistance of Salmonella cells in mono- and dual-species biofilms with Pseudomonas aeruginosa, and to investigate the role of extracellular polymeric substances (EPS) in protection of biofilms against disinfection treatment. METHODS AND RESULTS: The populations of Salmonella in mono- or dual-species biofilms with P. aeruginosa on stainless steel (SS) coupons were determined before and after exposure to commercial disinfectant, 50 μg ml(-1) chlorine or 200 μg ml(-1) Ecolab(®) Whisper(™) V (a blend of four effective quaternary ammonium compounds)...
June 23, 2017: Journal of Applied Microbiology
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