journal
https://read.qxmd.com/read/38533661/slightly-acidic-electrolyzed-water-significantly-restrains-the-accumulation-of-the-microalgae-pseudokirchneriella-subcapitata-in-hydroponic-systems
#1
JOURNAL ARTICLE
Cuinan Wu, Yuanyuan Li, Xue Wu, Encai Bao, Lin Ye, Kai Cao
AIMS: This study explored the effects of slightly acidic electrolyzed water (SAEW) on algae to exploit technologies that effectively suppress algal growth in hydroponic systems and improve crop yield. METHODS AND RESULTS: The effects of SAEW on algal growth and the response mechanisms of algae to SAEW were investigated. Moreover, we studied whether the application of SAEW adversely affected tomato seedling growth. The results showed that SAEW significantly inhibited algal growth and destroyed the integrity of the algal cells...
March 26, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38520165/virulent-klebsiella-pneumoniae-st11-clone-carrying-blakpc-and-blandm-from-patients-with-and-without-covid-19-in-brazil
#2
JOURNAL ARTICLE
Maria Izabely Silva Pimentel, Elizabeth Maria Bispo Beltrão, Érica Maria de Oliveira, Lamartine Rodrigues Martins, Moacir Batista Jucá, Ana Catarina de Souza Lopes
AIMS: Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil. METHODS AND RESULTS: All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, ERIC-PCR and MLST were used...
March 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38520159/non-thermal-plasma-air-disinfection-for-the-inactivation-of-airborne-microorganisms-in-an-experimental-chamber-and-indoor-air
#3
JOURNAL ARTICLE
Ye Li, Lanfen Wei, Junming Lin, Zhongyi Xie, Longxi Lu, Xieshang Pan, Ji Xu, Ran Cai
AIMS: Airborne transmission of diseases presents a serious threat to human health, so effective air disinfection technology to eliminate microorganisms in indoor air is very important. This study evaluated the effectiveness of a non-thermal plasma (NTP) air disinfector in both laboratory experiments and real environments. METHODS AND RESULTS: An experimental chamber was artificially polluted with a bioaerosol containing bacteria or viruses. Additionally, classroom environments with and without people present were used in field tests...
March 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38520154/bacteriological-culture-and-direct-pcr-for-detecting-the-mycobacterium-tuberculosis-complex-in-the-italian-eradication-campaign-a-decade-of-experience-at-the-national-reference-laboratory
#4
JOURNAL ARTICLE
Stefania Ferrari, Mariagrazia Zanoni, Anna Mangeli, Claudio Pigoli, Mario D'Incau, Giovanni Loris Alborali, Maria Lodovica Pacciarini, Maria Beatrice Boniotti
AIMS: Our study evaluates the capacity of direct real-time PCR for detecting Mycobacterium tuberculosis complex (MTBC), with a focus on diagnostic performances and the feasibility of implementing this protocol in an eradication campaign. Specifically, we compare the effectiveness of the direct PCR method to various culture systems used by the Italian National Reference Laboratory over the last decade to detect MTBC. METHODS AND RESULTS: Bovine tissue samples were routinely tested and analyzed for bovine Tuberculosis (bTB) confirmation using microbiological culture (solid and liquid media), histopathological analysis, and a direct PCR assay targeting IS6110, an insertion sequence specific to the MTBC that is widely used for tuberculosis diagnosis...
March 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38520150/seed-borne-bacterial-synthetic-community-resists-seed-pathogenic-fungi-and-promotes-plant-growth
#5
JOURNAL ARTICLE
Luo De-Lin, Shi-Yi Huang, Chen-Yu Ma, Xiang-Yu Zhang, Kai Sun, Wei Zhang, Chuan-Chao Dai
AIMS: In this study, the control effects of synthetic microbial communities composed of peanut seed bacteria against seed aflatoxin contamination caused by Aspergillus flavus and root rot by Fusarium oxysporum were evaluated. METHODS AND RESULTS: Potentially conserved microbial synthetic community (C), growth-promoting synthetic community (S), and combined synthetic community (CS) of peanut seeds were constructed after 16 S rRNA Illumina sequencing, strain isolation, and measurement of plant growth promotion indicators...
March 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38515285/comparative-phenotypic-and-genotypic-analysis-of-community-acquired-and-hospital-acquired-intra-abdominal-infections-among-liver-transplanted-patients
#6
JOURNAL ARTICLE
Arwa R Elmanakhly, Nesreen Safwat, Sally T K Tohamy, Khaled M Elsayed, Heba M ElSherif, Mahitab Elsayed, Reham M El-Tarabili, Majid Alhomrani, Abdulhakeem S Alamri, Mohammed M Ghoneim, Mohammed Abdulrahman Alshahrani, Ahmed Saif, Abdallah Tageldein Mansour, Mahmoud M Bendary
AIM: During liver transplantation, both hospital-acquired (HA) and community-acquired (CA) intra-abdominal infections (IAIs) are involved causing life-threatening diseases. Therefore, comparative studies of aerobic and facultative anaerobic HA-IAIs and CA-IAIs after liver transplantation surgery are necessary. METHODS AND RESULTS: the species of detected isolates (310) from intra-abdominal fluid were identified and classified into HA-IAIs and CA-IAIs. Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii were the most commonly detected species...
March 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38509027/isolation-and-genome-characterization-of-paenibacillus-polymyxa-188-a-potential-biocontrol-agent-against-fungi
#7
JOURNAL ARTICLE
Sra-Yh Shih, You-Syu Huang, Ker-Rui Chou, Hung-Yi Wu, HsinYuan Tsai
AIMS: In this work, we aimed to isolate marine bacteria that produce metabolites with antifungal properties. METHODS AND RESULTS: Paenibacillus polymyxa 188 was isolated from a marine sediment sample and it showed excellent antifungal activity against many fungi pathogenic to plants (Fusarium tricinctum, Pestalotiopsis clavispora, Fusarium oxysporum, Fusarium oxysporum f. sp. Cubense (Foc), Curvularia plantarum, and Talaromyces pinophilus) and to humans (Aspergillus terreus, Penicillium oxalicum, and Microsphaeropsis arundinis)...
March 20, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38509024/specifically-targeting-antimicrobial-peptides-for-inhibition-of-candidatus-liberibacter-asiaticus
#8
JOURNAL ARTICLE
Samavath Mallawarachchi, Haoqi Wang, Nirmitee Mulgaonkar, Sonia Irigoyen, Carmen Padilla, Kranthi Mandadi, James Borneman, Sandun Fernando
AIMS: Huanglongbing (citrus greening) is a plant disease putatively caused by the unculturable gram-negative bacterium Candidatus Liberibacter asiaticus (CLas), and it has caused severe damage to citrus plantations worldwide. There are no definitive treatments for this disease, and conventional disease control techniques have shown limited efficacy. This work presents an in-silico evaluation of using Specifically Targeting Anti-Microbial Peptides (STAMPs) consisting of a targeting segment and an antimicrobial segment to inhibit citrus greening by inhibiting the BamA protein of CLas, which is an outer membrane protein crucial for bacterial viability...
March 20, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38503568/antimicrobial-and-antibiofilm-activity-of-highly-soluble-polypyrrole-against-methicillin-resistant-staphylococcus-aureus
#9
JOURNAL ARTICLE
Danillo Sales Rosa, Samily Aquino de Sá Oliveira, Renata de Faria Silva Souza, Chirles Araujo de França, Isabelle Caroline Pires, Márcio Rennan Santos Tavares, Helinando Pequeno de Oliveira, Fernando Antônio Gomes da Silva Júnior, Maria Aparecida Scatamburlo Moreira, Mariana de Barros, Gustavo Batista de Menezes, Maísa Mota Antunes, Vasco Ariston de Carvalho Azevedo, Carine Rosa Naue, Mateus Matiuzzi da Costa
INTRODUCTION: As a new promising therapeutic option, the activity of highly soluble polypyrrole (Hs-PPy), a specific formulation of a conducting polymer (polypyrrole - PPy) is evaluated against bacteria of clinical importance. AIMS: The purpose was to evaluate the antimicrobial activity of Hs-PPy, alone or combined with oxacillin, as well as its antibiofilm potential against methicillin-resistant Staphylococcus aureus strains. Furthermore, the in silico inhibitory mechanism in efflux pumps was also investigated...
March 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38503567/wheat-rhizosphere-dynamics-of-trichoderma-gamsii-a5mh-and-suppression-of-a-pythium-root-rot-fusarium-crown-rot-disease-complex-over-two-consecutive-cropping-seasons
#10
JOURNAL ARTICLE
Belinda E Stummer, Xinjian Zhang, Minoo J Moghaddam, Hetong Yang, Paul R Harvey
AIMS: Determine the wheat rhizosphere competence of Trichoderma gamsii strain A5MH and in planta suppression of the Pythium root and Fusarium crown rot pathogens Globisporangium irregulare and Fusarium pseudograminearum. METHODS AND RESULTS: Wheat was continuously cropped (eight years) at a minimum tillage, low growing season rainfall (GSR ≤ 170 mm) site shown as highly conducive to Pythium root and Fusarium crown rots. Root isolation frequency (RIF) and qPCR was used to determine the rhizosphere dynamics of strain A5MH and the target pathogens at tillering, grain harvest and in postharvest stubble over the final two years...
March 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38503565/exploring-the-biocontrol-potential-of-rocket-eruca-sativa-extracts-and-associated-microorganisms-against-verticillium-wilt
#11
JOURNAL ARTICLE
Eirini G Poulaki, Ioanna Karamichali, Orestis Lianos, Vasilis Alexopoulos, Vasilis Dimitrakas, Grigorios G Amourgis, Sotirios E Tjamos
AIMS: This study aimed to assess the impact of rocket (Eruca sativa) extract on Verticillium wilt in eggplants, explore rhizospheric microorganisms for disease biocontrol, and evaluate selected strains' induced systemic resistance potential while characterizing their genomic and biosynthetic profiles. METHODS AND RESULTS: Rocket extract application led to a significant reduction in Verticillium wilt symptoms in eggplants compared to controls. Isolated microorganisms from treated soil, including Paraburkholderia oxyphila EP1, Pseudomonas citronellolis EP2, Paraburkholderia eburnea EP3, and Paraburkholderia oxyphila EP4 and EP5, displayed efficacy against Verticillium dahliae, decreasing disease severity and incidence in planta...
March 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38486365/peribacillus-frigoritolerans-t7-iitj-a-potential-biofertilizer-induces-plant-growth-promoting-genes-of-arabidopsis-thaliana
#12
JOURNAL ARTICLE
Debankona Marik, Pinki Sharma, Nar Singh Chauhan, Neelam Jangir, Rajveer Singh Shekhawat, Devanshu Verma, Manasi Mukherjee, Moses Abiala, Chandan Roy, Pankaj Yadav, Ayan Sadhukhan
AIMS: This study aimed to isolate plant growth and drought tolerance-promoting bacteria from the nutrient-poor rhizosphere soil of Thar desert plants and unravel their molecular mechanisms of plant growth promotion. METHODS AND RESULTS: Among our rhizobacterial isolates, Enterobacter cloacae C1P-IITJ, Kalamiella piersonii J4-IITJ, and Peribacillus frigoritolerans T7-IITJ, significantly enhanced root and shoot growth (4-5-fold) in Arabidopsis thaliana under PEG-induced drought stress...
March 14, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38486355/enhancing-a-multi-purpose-artificial-urine-for-culture-and-gene-expression-studies-of-uropathogenic-escherichia-coli-strains
#13
JOURNAL ARTICLE
Patricia T Rimbi, Nicky O'Boyle, Gillian R Douce, Mariagrazia Pizza, Roberto Rosini, Andrew J Roe
AIMS: The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. METHODS AND RESULTS: We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU...
March 14, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38486350/smelly-shark-smelly-ray-what-is-infecting-you
#14
JOURNAL ARTICLE
Maíra Duarte Cardoso, Oswaldo Luiz de C Maciel, André Luiz Medeiros de Souza, Emily Moraes Roges, Verônica Dias Gonçalves, Salvatore Siciliano, Dália Dos Prazeres Rodrigues, Rachel Ann Hauser-Davis
AIMS: Although elasmobranchs are consumed worldwide, bacteriological assessments for this group are still sorely lacking. In this context, this study assessed bacteria of sharks and rays from one of the most important landing ports along the Rio de Janeiro coast. METHODS AND RESULTS: Bacteria were isolated from the cloacal swabs of the sampled elasmobranchs. They were cultured and Vibrio, Aeromonas and Enterobacterales were isolated and identified. The isolated bacteria were then biochemically identified and antimicrobial susceptibility assays were performed...
March 14, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38471695/assessing-silver-nanoparticle-and-antimicrobial-combinations-for-antibacterial-activity-and-biofilm-prevention-on-surgical-sutures
#15
JOURNAL ARTICLE
Diana K Al-Sawarees, Rula M Darwish, Rund Abu-Zurayk, Mahmoud Al Masri
AIMS: To evaluate the effect of silver nanoparticles alone and in combination with Triclosan, and trans-cinnamaldehyde against S.aureus and E.coli biofilms on sutures to improve patients' outcomes. METHODS AND RESULTS: Silver nanoparticles were prepared by chemical method and characterized by UV-visible spectrophotometer and dynamic light scattering. The minimum inhibitory concentration was assessed by the Microdilution assay. The antibiofilm activity was determined using crystal violet assay...
March 12, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38467395/antimicrobial-resistance-%C3%AE-lactamase-genotypes-and-plasmid-replicon-types-of-shiga-toxin-producing-escherichia-coli-isolated-from-different-animal-hosts
#16
JOURNAL ARTICLE
Rwida Tomeh, Ali Nemati, Gholamreza Hashemi Tabar, Rosangela Tozzoli, Mahdi Askari Badouei
AIMS: The primary objective of this study was to analyze antimicrobial resistance (AMR), with a particular focus on β-lactamase genotypes and plasmid replicon types of STEC strains originating from various animal hosts. METHODS AND RESULTS: A total of 84 STEC strains were isolated from cattle (n=32), sheep/goats (n=26), pigeons (n=20), and wild animals (n=6) between 2010 and 2018 in various regions of Iran. The Kirby-Bauer susceptibility test and multiple polymerase chain reaction (PCR) panels were employed to elucidate the correlation between AMR and plasmid replicon types in STEC isolates...
March 11, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38467390/in-vitro-and-in-silico-analysis-unravelled-clinically-desirable-attributes-of-bacillus-altitudinis-l-asparaginase
#17
JOURNAL ARTICLE
V P Lailaja, Vishnu Hari, T G Sumithra, V N Anusree, Gayathri Suresh, N K Sanil, S R Krupesha Sharma, A Gopalakrishnan
AIMS: To identify a marine L-asparaginase with clinically desirable attributes and characterize the shortlisted candidate through in silico tools. METHODS AND RESULTS: Marine bacterial strains (number=105) isolated from marine crabs were evaluated through a stepwise strategy incorporating the crucial attributes for therapeutic safety. The results demonstrated the potential of eight bacterial species for extracellular L-asparaginase production. However, only one isolate (B...
March 11, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38460954/investigation-of-the-seasonal-prevalence-phenotypic-and-genotypic-characteristics-of-listeria-monocytogenes-in-slaughterhouses-in-burdur
#18
JOURNAL ARTICLE
Zeki Erol, Fulya Taşçı
AIM: This study examined Listeria monocytogenes isolates from two slaughterhouses in Burdur province, southern Turkey, over four seasons for antibiotic resistance, serogroups, virulence genes, in vitro biofilm forming capacity, and genetic relatedness. METHODS AND RESULTS: Carcass (540) and environment-equipment surface (180) samples were collected from two slaughterhouses (S1, S2) for 1 year (4 samplings). Of the 89 (12.4%) positive isolates, 48 (53.9%) were from animal carcasses and 41 (46...
March 9, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38458234/impact-of-dry-density-and-incomplete-saturation-on-microbial-growth-in-bentonite-clay-for-nuclear-waste-storage
#19
JOURNAL ARTICLE
Rachel C Beaver, Melody A Vachon, Claire S Tully, Katja Engel, Emilie Spasov, W Jeffrey Binns, James J Noël, Josh D Neufeld
AIMS: Many countries are in the process of designing a deep geological repository (DGR) for long-term storage of used nuclear fuel. For several designs, used fuel containers will be placed belowground with emplacement tunnels being backfilled using a combination of highly compacted powdered bentonite clay buffer boxes surrounded by a granulated "gapfill" bentonite. To limit the potential for microbiologically influenced corrosion of used fuel containers, identifying conditions that suppress microbial growth is critical for sustainable DGR design...
March 8, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38449343/effect-of-citric-acid-on-cell-membrane-structure-and-function-of-issatchenkia-terricola-wjl-g4
#20
JOURNAL ARTICLE
Xiangfeng Meng, Xinyi Liu, Yihong Bao, Ting Luo, Jinling Wang
AIMS: This study aimed to investigate the changes of cell membrane structure and function of Issatchenkia terricola under citric acid by performing physiological analysis. METHODS AND RESULTS: The membrane integrity, surface hydrophobicity, structure, fluidity, apoptosis and fatty acid methyl esters composition of I. terricola WJL-G4 cells were determined by propidium iodide staining, microbial adhesion to hydrocarbon test, transmission electron microscopy analysis, fluorescence anisotropy, flow cytometry and gas chromatography-mass, respectively...
March 6, 2024: Journal of Applied Microbiology
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