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Journal of Applied Microbiology

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https://www.readbyqxmd.com/read/28236643/selection-of-indigenous-lactobacillus-paracasei-cd4-and-lactobacillus-gastricus-btm-7-as-probiotic-assessment-of-traits-combined-with-principal-component-analysis
#1
Kavita Sharma, Rishi Mahajan, Sampan Attri, Gunjan Goel
AIMS: The population of Himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. Therefore, the focus of the present study was to identify new strains of Lactic Acid Bacteria (LAB) from dairy products such as milk (cow, goat, buffalo) and fermented products (curd and buttermilk) with properties suitable for use as probiotic cultures. METHODS AND RESULTS: A total of 75 isolates tentatively identified as LAB from 100 samples were initially screened for production of β-hemolysin as indicators of virulence which resulted in 38 isolates with no haemolytic activity...
February 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28236353/evaluation-of-growth-conditions-and-dna-extraction-techniques-used-in-the-molecular-analysis-of-dermatophytes
#2
Sebastian Gnat, Aneta Nowakiewicz, Grażyna Ziółkowska, Aleksandra Trościańczyk, Barbara Majer-Dziedzic, Przemysław Zięba
AIMS: Recent molecular methods for diagnosis of superficial mycoses have determined the need for a rapid and easy method of extracting DNA. The aim of study was to determine growth conditions and techniques of DNA extraction for Microsporum canis, Trichophyton mentagrophytes, and T. verrucosum. METHODS AND RESULTS: Samples were prepared of each of the DNA extraction methods (phenol-chloroform, CTAB, and four different kits) for all of the incubation periods (four, seven, and 10 d) of the cultures on the solid and in the liquid medium...
February 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28235155/sensitive-and-specific-detection-of-viable-mycobacterium-avium-subsp-paratuberculosis-in-raw-milk-by-the-peptide-mediated-magnetic-separation-pms-phage-assay
#3
Antonio C G Foddai, Irene R Grant
AIM: To validate an optimised Peptide-mediated magnetic separation (PMS)-Phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis (MAP) in milk. METHODS AND RESULTS: Inclusivity, specificity and limit of detection 50% (LOD50 ) of the optimised PMS-phage assay were assessed. Plaques were obtained for all 43 MAP strains tested. Of 12 other Mycobacterium spp. tested, only M. bovis BCG produced small numbers of plaques. LOD50 of the PMS-phage assay was 0...
February 24, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28231617/characterization-of-staphylococcus-epidermidis-strains-isolated-from-industrial-cleanrooms-under-regular-routine-disinfection
#4
U Ribič, A Klančnik, B Jeršek
AIMS: The purpose of this study was the genotypic and phenotypic characterization of 57 strains of Staphylococcus epidermidis isolated from cleanroom environments, based on their biofilm formation and antimicrobial resistance profiles. METHODS AND RESULTS: Biofilm formation was investigated using real-time PCR (icaA, aap, bhp genes), the Congo red agar method, and the crystal violet assay. The majority of the strains (59.7%; 34/57) did not form biofilms according to the crystal violet assay, although the biofilm-associated genes were present in 94...
February 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28214364/in-situ-analysis-of-bacillus-licheniformis-biofilms-amyloid-like-polymers-and-edna-are-involved-in-the-adherence-and-aggregation-of-the-extracellular-matrix
#5
I Randrianjatovo-Gbalou, P Rouquette, D Lefebvre, E Girbal-Neuhauser, C-E Marcato-Romain
AIMS: This study attempts to determine which exopolymeric substances are involved in the adherence and aggregation of a Bacillus licheniformis biofilm. METHODS AND RESULTS: The involvement of extracellular proteins and eDNA were particularly investigated using DNase and proteinase K treatment. The permeability of the biofilms increased 5-fold after DNase I-treatment. Quantification of the matrix components showed that, whatever the enzyme tested, some eDNA and amyloid-like polymers were removed simultaneously...
February 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28208239/narcissus-yellow-stripe-virus-and-narcissus-mosaic-virus-detection-in-narcissus-via-multiplex-taqman-based-reverse-transcription-pcr-assay
#6
Jing Jin, Jianguo Shen, Wei Cai, Xie Guohui, Furong Liao, Fangluan Gao, Junfeng Ma, Xihong Chen, Zujian Wu
AIMS: Development of a multiplex TaqMan RT-qPCR assay to simultaneously detect Narcissus yellow stripe virus (NYSV) and Narcissus mosaic virus (NMV), frequently causing mixed narcissus infection. Feasibility verification was confirmed in natural samples. METHODS AND RESULTS: Primers and probes were designed based on the conserved CP gene regions of NYSV or NMV and their suitability for singleplex and multiplex TaqMan RT-qPCR assays as well as for conventional RT-PCR...
February 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28199767/alkaliphilic-bacillus-species-show-potential-application-in-concrete-crack-repair-by-virtue-of-rapid-spore-production-and-germination-then-extracellular-calcite-formation
#7
T K Sharma, M Alazhari, A Heath, K Paine, R M Cooper
AIMS: Characterisation of alkaliphilic Bacillus species for spore production and germination and calcite formation as a prelude to investigate their potential in micro-crack remediation in concrete. METHODS AND RESULTS: Conditions, extent and timing of endospore production was determined by dark field light microscopy; germination induction and kinetics was assessed by combining reduction in optical density with formation of refractile bodies by phase contrast microscopy...
February 15, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28199757/genetic-enzymatic-and-metabolite-profiling-of-the-lactobacillus-casei-group-reveals-strain-biodiversity-and-potential-applications-for-flavour-diversification
#8
Ewelina Stefanovic, Kieran N Kilcawley, Mary C Rea, Gerald F Fitzgerald, Olivia McAuliffe
AIMS: The Lactobacillus casei group represents a widely explored group of lactic acid bacteria, characterised by a high level of biodiversity. In this study, the genetic and phenotypic diversity of a collection of more than 300 isolates of the L. casei group and their potential to produce volatile metabolites important for flavour development in dairy products was examined. METHODS AND RESULTS: Following confirmation of species by 16S rRNA PCR, the diversity of the isolates was determined by pulsed field gel electrophoresis...
February 15, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28194914/genetic-diversity-and-antimicrobial-resistance-profiles-of-campylobacter-coli-and-campylobacter-jejuni-isolated-from-broiler-chicken-in-farms-and-at-slaughter-in-central-italy
#9
S Pergola, M P Franciosini, F Comitini, M Ciani, S De Luca, S Bellucci, L Menchetti, P Casagrande Proietti
AIMS: Genetic diversity and antimicrobial resistance of C. coli and C. jejuni were investigated along the broiler chicken production chain in central Italy. METHODS AND RESULTS: Campylobacter spp. isolated from cloacal swabs in farms (n = 116) and from neck skin of chilled and eviscerated carcasses at slaughter (n = 24) were identified as C. coli (n = 99) and C. jejuni (n = 41) by multiplex PCR. Characterisation by s-AFLP revealed a specific genotype of Campylobacter for each farm...
February 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28191745/comparison-of-sampling-methods-to-recover-germinated-bacillus-anthracis-and-bacillus-thuringiensis-endospores-from-surface-coupons
#10
Tiffany M Mott, Jennifer L Shoe, Melissa Hunter, Amber M Woodson, Kristen A Fritts, Christopher P Klimko, Avery V Quirk, Susan L Welkos, Christopher K Cote
AIMS: In an attempt to devise decontamination methods that are both effective and minimally detrimental to the environment, we evaluated germination-induction as an enhancement to strategies for Bacillus anthracis spore decontamination. To determine an optimal method for the recovery of germinating spores from different matrices it was critical to ensure that the sampling procedures did not negatively impact the viability of the germinating spores possibly confounding the results and downstream analyses of field trial data...
February 12, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28186676/acc-deaminase-containing-plant-growth-promoting-rhizobacteria-protect-papaver-somniferum-from-downy-mildew
#11
Deepti Barnawal, Shiv Shanker Pandey, Nidhi Bharti, Alok Pandey, Tania Ray, Sucheta Singh, Chandan Singh Chanotiya, Alok Kalra
AIMS: The aim of this study was to determine whether ACC deaminase-containing bacterial treatments could enhance the tolerance of poppy (Papaver somniferum L.) plants against biotic stress of downy mildew caused by Peronospora spp. METHODS AND RESULTS: Three different genotypes of P. somniferum i.e. Sampada, J-16 and I-14 were included in the experiment. The ACC deaminase-containing bacteria Pseudomonas putida (WPTe) reduced the downy mildew disease severity and significantly improved the growth and yield of P...
February 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28177186/characterization-of-enterococcus-faecalis-isolated-from-the-cloaca-of-fancy-breeds-and-confined-chickens
#12
Steffen Lynge Jørgensen, Louise Ladefoged Poulsen, Lotte Thorndal, Amir Atabak Ronaghinia, Magne Bisgaard, Henrik Christensen
AIMS: The aim was to investigate the intestinal community of E. faecalis from healthy confined non-industrialized chicken. METHODS AND RESULTS: A total of 206 E. faecalis isolates were collected from cloacal swabs. The prevalence of E. faecalis from two confined flocks, were 83% and 96%, while only 44% and 13% in two fancy breeder flocks. A total of 204 isolates were characterized by pulsed-field-gel-electrophoresis (PFGE) where 40 strains were selected for multi locus sequence typing (MLST)...
February 8, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28176460/application-of-bacteria-from-non-cultivated-plants-to-promote-growth-alter-root-architecture-and-alleviate-salt-stress-of-cotton
#13
Ivelisse Irizarry, James F White
AIMS: Cotton seeds are frequently treated with acid to remove fibers and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth promoting and salt stress alleviating effects in seedlings. METHODS AND RESULTS: Bacteria were isolated from non-cultivated plants in the Malvaceae. Seeds were collected from Portia tree (Thespesia populnea) and wild cotton (Gossypium hirsutum) from coastal and arid areas of Puerto Rico...
February 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28176439/origanum-vulgare-essential-oil-affects-pathogens-causing-vaginal-infections
#14
Maja Karaman, Mirjana Bogavac, Bojan Radovanović, Jan Sudji, Kristina Tešanović, Ljiljana Janjušević
AIMS: This study aimed to investigate the application of Origanum vulgare L. essential oil (EO) as alternative antimicrobial agent against vaginal infections. METHODS AND RESULTS: Microdilution methods were applied for detection of minimal inhibitory (MIC) and minimal bactericidal/fungicidal MBC (MFC) concentration on 15 clinical strains originated from women with symptoms of vaginal infection. Optical density determination (OD) was used for detecton of Candida growth rate...
February 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28176435/fish-intestinal-microbiome-diversity-and-symbiosis-unraveled-by-metagenomics
#15
REVIEW
Andrea M Tarnecki, Francisca A Burgos, Candis L Ray, Covadonga R Arias
The gut microbiome of vertebrates plays an integral role in host health by stimulating development of the immune system, aiding in nutrient acquisition, and outcompeting opportunistic pathogens. Development of next-generation sequencing (NGS) technologies allows researchers to survey complex communities of microorganisms within the microbiome at great depth with minimal costs, resulting in a surge of studies investigating bacterial diversity of fishes. Many of these studies have focused on the microbial structure of economically-significant aquaculture species with the goal of manipulating the microbes to increase feed efficiency and decrease disease susceptibility...
February 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28160353/a-review-on-microbiological-decontamination-of-fresh-produce-with-nonthermal-plasma
#16
REVIEW
C Pignata, D D'Angelo, E Fea, G Gilli
Food safety is a critical public health issue for consumers and the food industry because microbiological contamination of food causes considerable social and economic burdens on health care. Most foodborne illness comes from animal production, but as of the mid-1990s in the United States and more recently in the European Union, the contribution of fresh produce to foodborne outbreaks has rapidly increased. Recent studies have suggested that sterilisation with nonthermal plasma could be a viable alternative to the traditional methods for the decontamination of heat-sensitive materials or food because this technique proves capable of eliminating microorganisms on surfaces without altering the substrate...
February 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28160348/phoma-crystallifera-with-phytotoxic-effects-and-pathogenic-potential-against-field-bindweed-convolvulus-arvensis-l-in-iran
#17
Parisa Razaghi, Doustmorad Zafari
AIMS: To identify a potential pathogenic isolate of fungus on Convolvulus arvensis and to determine its phytotoxic activity, which revealed the presence of toxic metabolites responsible for the toxicity against the target weed. METHODS AND RESULTS: A high virulent isolate of the fungus, Phoma crystallifera was isolated from symptomatic field bindweed in the west of Iran and was screened for the production of phytotoxins, which promoted necrosis on the detached leaves and seedlings of field bindweed in the bioassays...
February 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28150900/discovery-of-antimicrobial-and-antitubercular-molecules-from-fusarium-solani-an-endophyte-of-glycyrrhiza-glabra
#18
Aiyatullah Shah, Muzafar Ahmad Rather, Parvaiz H Qazi, Mushtaq A Aga, Saleem Mushtaq, Aabid Manzoor Shah, Aehtesham Hussain, Shoib A Baba, Zahoor Ahmad
AIM: Glycyrrhiza glabra is a high value medicinal plant thriving in biodiversity rich Kashmir Himalaya. The present study was designed to explore the fungal endophytes from G glabra as a source of bioactive molecules. METHODS AND RESULTS: The extracts prepared from the isolated endophytes were evaluated for antimicrobial activities using broth micro-dilution assay. The endophytic strain coded as A2 exhibiting promising antibacterial as well as antituberculosis activity was identified as Fusarium solani by ITS-5...
February 2, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28150898/biofilm-formation-invasiveness-and-colicinogeny-in-locus-of-enterocyte-and-effacement-negative-o113-h21-shigatoxigenic-escherichia-coli
#19
Luís Fernando Dos Santos, Franciele Tafarello Biscola, Evanilde Maria Gonçalves, Beatriz Ernestina Cabílio Guth
AIMS: Although Shiga toxins (Stx) are well established virulence traits of O113:H21 Shigatoxigenic Escherichia coli (STEC) strains, a shortage in the knowledge of other virulence properties that may contribute to pathogenesis may exist in this serotype. This study investigated biofilm, invasiveness and colicinogeny capabilities in O113:H21 STEC isolated in Brazil, mostly from animal reservoirs. A search for genes which were reported to participate in the process of biofilm formation was also performed...
February 2, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28135780/thyme-essential-oil-as-multiple-mechanisms-biofungicide-causing-sensitivity-of-mycosphaerella-graminicola
#20
Maissa Ben Jabeur, Lamia Somai Jemmali, Walid Hamada
AIMS: To understand the mode of action of thyme essential oil as an alternative biofungicide. METHODS AND RESULTS: The chemical composition of thyme essential oil isolated by hydrodistillation from the aerial parts of Thymus vulgaris was analyzed. The main constituents of thyme essential oil were thymol (76.96%), ρ-cymene (9.89%), γ-terpinene (1.92%) and caryophyllene oxide (1.69%). The antifungal activity of the oil and its pure major component (thymol) was assessed by the in vitro assay against Mycosphaerella graminicola...
January 30, 2017: Journal of Applied Microbiology
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