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Journal of Applied Microbiology

Hongyi Qin, Cheng Han, Zhiwei Jin, Ling Wu, Huan Deng, Guangwei Zhu, Wenhui Zhong
AIMS: The aim of this study was to explore the vertical distribution traits of anaerobic ammonium-oxidizing (anammox) bacterial relative abundance and community composition along the oxic/anoxic sediment profiles in a shallow lake. METHODS AND RESULTS: The Illumina Miseq-based sequencing and quantitative polymerase chain reactions were utilized to analyze relative abundance of anammox hydrazine synthase (hzsβ) gene in comparison with bacterial 16S rRNA genes, anammox bacterial relative abundance (the number of anammox sequences divided by total number of sequences), community composition and diversity in sediments...
March 15, 2018: Journal of Applied Microbiology
Archer Ann Catherine, Nawneet K Kurrey, Prakash M Halami
AIM: This study aimed at characterizing the adhesion and immune-stimulatory properties of native probiotic Lactobacillus fermentum (MCC2759 and MCC2760) and Lactobacillus delbrueckii MCC2775. METHODS AND RESULTS: Adhesion of the strains were assessed in Caco-2 and HT-29 cell lines. Expression of adhesion and immune markers were evaluated in Caco-2 cells by real-time qPCR. The cultures displayed >80% of adhesion to both cell lines and also induced the expression of mucin binding protein (mub) gene in presence of mucin, bile and pancreatin...
March 14, 2018: Journal of Applied Microbiology
A Deethae, Y Peerapornpisal, J Pekkoh, P Sangthong, Y Tragoolpua
AIMS: To determine the antiviral activities of Spirogyra spp. algal extracts against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). METHODS AND RESULTS: Spirogyra spp. was extracted using water, ethanol and methanol. Aqueous extract of Spirogyra spp. had the lowest toxicity on Vero cells with the 50% cytotoxicity concentration (CC50 ) of 4363·30 μg ml-1 . As for potent inhibitory effect, the ethanolic extract presented the highest inhibition of viral infection on HSV-1 in the treatment during viral attachment on Vero cells with 50% inhibitory concentration (IC50 ) and selective index (SI) values of 164·20 and 2·17 μg ml-1 ...
March 13, 2018: Journal of Applied Microbiology
K Soumya, K Narasimha Murthy, G L Sreelatha, T Sharmila
AIM: This research aims to characterize the pigment produced by isolated fungi and to evaluate its anti-cancer activities. METHODS AND RESULTS: Pigment producing fungi was isolated and identified as Fusarium chlamydosporum. The pigment was extracted with chloroform, purified by PTLC and characterized by FT-IR, ESIMS, LCMS and NMR (1 HNMR,13 C NMR) spectral analysis, which revealed the pigment to be "long chain hydrocarbons with poly unsaturated groups" (m/z 702)...
March 12, 2018: Journal of Applied Microbiology
Sara H Mohamed, Mahmoud S M Mohamed, Mary S Khalil, Magda Azmy, Mona I Mabrouk
AIM: This study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae METHODS AND RESULTS: Effect of seven EOs and three enzymes was tested on formation and eradication of Klebsiella pneumoniae biofilm. Peppermint oil showed robust biofilm inhibitory effect, causing inhibition ranged from 69.2-98.2% at 5 μl ml-1 . Thyme oil was found to have the best biofilm-eradication ability, causing eradication ranged from 80...
March 8, 2018: Journal of Applied Microbiology
Karina Griffin, Philip Brown, Cherie Gambley
AIMS: There are inconsistencies with in vitro copper-tolerance screening methodology for Pseudomonas syringae pv. tomato in the current literature, particularly in relation to the appropriate medium to use, copper-tolerance thresholds and reporting medium pH and/or pH adjustment steps. This study investigates the effect of medium and pH on copper-tolerance results, including the potential use of 2-(N-morpholino)ethanesulfonic acid (MES) buffer to stabilise media pH. METHODS AND RESULTS: Copper-tolerance methodology was investigated through in vitro and in vivo testing of P...
March 7, 2018: Journal of Applied Microbiology
Preeti Verma, Vijay Veer Saharan, Surendra Nimesh, Arvind Pratap Singh
AIMS: The present study was designed to assess the phenotypic traits and virulence determinants of vegetables/fruits-origin E. coli and Salmonella strains. METHODS AND RESULTS: A total of 520 fresh vegetables/fruits samples were analyzed for the presence of E. coli, including Shiga-toxin producing E. coli (STEC), and Salmonella. The vegetables/fruits-origin E. coli and Salmonella strains were further assessed for antimicrobial resistance, biofilm formation, extracellular matrix production, and in-vitro invasion/intracellular survivability assays...
March 7, 2018: Journal of Applied Microbiology
Jan Van Impe, Cindy Smet, Brijesh Tiwari, Ralf Greiner, Shikha Ojha, Višnja Stulić, Tomislava Vukušić, Anet Režek Jambrak
Despite of the constant development of novel thermal and non-thermal technologies, knowledge on the mechanisms of microbial inactivation is still very limited. Technologies such as high pressure, ultraviolet light, pulsed light, ozone, power ultrasound and cold plasma (advanced oxidation processes) have shown promising results for inactivation of microorganisms. The efficacy of inactivation is greatly enhanced by combination of conventional (thermal) with non-thermal, or non-thermal with another non-thermal technique...
March 4, 2018: Journal of Applied Microbiology
Catherine Feuillolay, Laila Haddioui, Marc Verelst, Aurelie Furiga, Loic Marchin, Christine Roques
AIMS: To investigate the potent control of microbial surface contamination of an innovative process which consists in incorporating metal oxide microspheres homogeneously into materials. METHODS AND RESULTS: Spherical microspheres containing zinc and magnesium oxides synthesized via a one-step manufacturing process (Pyrolyse Pulvérisée® ) and incorporated into different plastic matrices were evaluated for their antimicrobial activity according to JIS Z 2801 standard...
March 4, 2018: Journal of Applied Microbiology
John Mills, Kylie Marree Horváth, Angela Denise Reynolds, Gale Brightwell
AIMS: To investigate the transmission route of Carnobacterium from the farm environment to the meat-manufacturing plant and potential risk for meat spoilage. METHODS AND RESULTS: A sheep farm level survey of Carnobacterium, consisting of 150 environmental and animal (no 100) associated samples, was carried out on two farms. A further 20 lamb carcass samples were taken from an abattoir servicing one of the farms. The majority of C. maltaromaticum isolates were associated with fleece followed by hard sheep contact surfaces, rectal-anal-mucosal swabs and carcasses...
March 1, 2018: Journal of Applied Microbiology
A Novinscak, M Filion
AIM: This study was performed to identify bacterial isolates capable of enhancing total lipid and stearidonic acid (SDA) yields in Buglossoides arvensis. METHODS AND RESULTS: Pot experiments were conducted to screen the effects of 40 bacterial isolates on different B. arvensis growth parameters. Five isolates increased total lipid and SDA yields by at least 20%. These isolates were tested in a second pot experiment and in field trials. The second pot experiment confirmed that all isolates significantly increased total lipid and SDA yields over controls...
March 1, 2018: Journal of Applied Microbiology
Ioanna P Chatziprodromidou, Maria Bellou, George Vantarakis, Apostolos Vantarakis
AIMS: Α systematic review to investigate fresh produce-borne viral outbreaks, tο record the outbreak distribution worldwide and to analyze the implication of different types of fresh produce and viral types as well. METHODS AND RESULTS: Four databases (PubMed/Medline, Scopus, Eurosurveillance Journal and Spingerlink electronic journal) and a global electronic reporting system (ProMED) were searched up to 2016. One hundred and fifty two viral outbreaks linked to fresh produce consumption were identified...
February 27, 2018: Journal of Applied Microbiology
Hsin-Bai Yin, Xiangwu Nou, Ganyu Gu, Jitendra Patel
AIMS: The effect of reclaimed wastewater (RCW) and roof-harvest rainwater (RHW) on microbiological quality of irrigated spinach was investigated. METHODS AND RESULTS: Spinach grown in the controlled environment chamber was irrigated by RCW, RHW, or creek water (CW; control water) for four weeks, and then six replicate spinach samples from each treatment were collected weekly at 0 h and 24 h post-irrigation. Spinach samples were analyzed for populations of fecal bacterial indicators and pathogens...
February 25, 2018: Journal of Applied Microbiology
Amine Belmadani, Abdelhabib Semlali, Mahmoud Rouabhia
AIMS: This study aimed to investigate the effect of synthetic antimicrobial peptide dermaseptin-S1 (DS1) (ALWKTMLKKLGTMALHAGKAALGAAADTISQGTQ) on the growth of C. albicans, its transition from blastospore to hyphae, and its biofilm formation. We also analyzed the expression of different genes (HWP1 and SAPs) involved in C. albicans virulence. METHODS AND RESULTS: Using cell count we showed that in addition to decreasing C. albicans growth, peptide DS1 inhibited its transition from blastospore to hyphal form...
February 24, 2018: Journal of Applied Microbiology
L Madueño, B M Coppotelli, S Festa, H M Alvarez, Irma S Morelli
AIM: To analyze the physiological response of Sphingobium sp. 22B to water stress. METHODS AND RESULTS: The strain was grown under excess of carbon source and then submitted to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB), and transmission electron microscopy (TEM) was studied. Genes linked with desiccation were searched in Sphingobium sp. 22B and Sphingomonas "sensu latu" genomes and their transcripts were quantified by Real-Time PCR...
February 23, 2018: Journal of Applied Microbiology
Ivelisse Irizarry, James F White
AIMS: Previous research demonstrated that applying Bacillus amyloliquefaciens to cotton seeds promotes growth, alters root architecture, and alleviates salt stress of cotton seedlings. This research was undertaken to further study the genetic responses elicited in cotton seedlings by this growth promoting bacterium. METHODS AND RESULTS: GeneChip microarrays and RT-qPCR were used to detect changes in gene expression in seedling roots inoculated with B. amyloliquefaciens...
February 23, 2018: Journal of Applied Microbiology
Ronaldo A Pereira-Junior, Carla Huarte-Bonnet, Flávia R S Paixão, Donald W Roberts, Christian Luz, Nicolás Pedrini, Éverton K K Fernandes
AIMS: The effect of nutritional supplementation of two Metarhizium species with riboflavin (Rb) during production of conidia was (a) evaluated on conidial tolerance (based on germination) to UV-B radiation and (b) on conidial expression following UV-B irradiation, of enzymes known to be active in photoreactivation, viz., photolyase (Phr), laccase (Lcc) and polyketide synthase (Pks). METHODS AND RESULTS: Metarhizium acridum (ARSEF 324) and Metarhizium robertsii (ARSEF 2575) were grown either on (a) potato dextrose agar medium (PDA), (b) PDA supplemented with 1% yeast extract (PDAY), (c) PDA supplemented with Rb (PDA+Rb), or (d) PDAY supplemented with Rb (PDAY+Rb)...
February 23, 2018: Journal of Applied Microbiology
Srinivasan Ramanathan, Kannappan Arunachalam, Sivasankar Chandran, Rathika Selvaraj, Karutha Pandian Shunmugiah, Veera Ravi Arumugam
AIMS: This study aimed to evaluate the antibiofilm potential of phytol and cefotaxime combinations (PCCs) against A. baumannii and to elucidate the molecular mechanism of their antibiofilm potential through the transcriptomic approach. METHODS AND RESULTS: Phytol and cefotaxime combination(s) (PCC(s) [160 μg ml-1 + 8 μg ml-1 for microbial type culture collection (MTCC) strain and 160 μg ml-1 + 0.5 μg ml-1 for clinical isolate] effectively inhibited the A. baumannii biofilm formation...
February 23, 2018: Journal of Applied Microbiology
Ingrid Surono, Jessica Verhoeven, Sanne Verbruggen, Koen Venema
AIM: to test the effect of microencapsulation on the survival of two probiotic strains isolated from Dadih, Indonesian fermented buffalo milk, in a dynamic, computer controlled in vitro model of the upper gastrointestinal (GI) tract (TIM-1), simulating human adults. METHODS AND RESULTS: Free or microencapsulated probiotics, L.plantarum IS-10506 or E.faecium IS-27526, resuspended in milk were studied for survival in the complete TIM-1 system (stomach + small intestine) or in the gastric compartment of TIM-1 only...
February 23, 2018: Journal of Applied Microbiology
David Huyben, Li Sun, Richard Moccia, Anders Kiessling, Johan Dicksved, Torbjörn Lundh
AIMS: The objective was to determine the effects of dietary substitution of fishmeal with live yeast and increasing water temperature on the diversity and composition of gut microbiota in rainbow trout. METHODS AND RESULTS: Fish were fed either fishmeal or yeast (Saccharomyces cerevisiae) and reared in water temperatures of either 11 °C (cold) or 18 °C (warm) for six weeks. Luminal content and mucosa were collected from the distal gut and the load, diversity and species abundance of yeast and bacteria were analysed using agar plating, MALDI-TOF and rRNA gene amplicon sequencing...
February 21, 2018: Journal of Applied Microbiology
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