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Journal of Applied Microbiology

Ana Carolina do Prado, Hans Garcia Garces, Eduardo Bagagli, Vera Lucia Mores Rall, Alessandra Furlanetto, Ary Fernandes Junior, Fabiana Barcelos Furtado
AIMS: The study was focused on the evaluation of antimicrobial activity in vitro of the essential oil of leaves from S. molle against bacteria and fungi of clinical importance in the search for the discovery of new active compounds. METHODS AND RESULTS: The chemical composition of the S. molle essential oil was determined by gas chromatography/mass spectrometry and its antimicrobial effect was verified by broth microdilution method. The major compounds found were β-pinene (25...
November 15, 2018: Journal of Applied Microbiology
Anne Q Reuwer, Wouter van den Bijllaardt, Jean-Luc Murk, Anton G M Buiting, Jaco J Verweij
AIMS: Evaluation of 16S PCR additional to standard culture to improve the pathogen detection rate in clinical specimens. METHODS AND RESULTS: Microbiological culture and direct 16S PCR was performed on specimens from suspected prosthetic joint infection patients (cohort-1) and on tissues and fluids from other normally sterile body sites (cohort-2). Based on clinical and microbiological data the detection rate for both methods was assessed, assuming no superiority of either 16S PCR or culture...
November 15, 2018: Journal of Applied Microbiology
Fatma Ibrahim Sonbol, Tarek El-Said El-Banna, Ahmed Ahmed Abd El-Aziz, Engy El-Ekhnawy
AIM: Analysing the effect of step-wise exposure of Escherichia coli isolates to sub lethal concentrations of triclosan (TCS) that is widely used as antiseptic, preservative and disinfectant. METHODS AND RESULTS: The analysis included the changes in the tolerance to the biocide itself and the cross-resistance to clinically important antibiotics. The involvement of efflux mechanism was studied as well as the possible implication of modifications in cytoplasmic membrane properties including integrity, permeability, potential and depolarization in the resistance mechanisms...
November 15, 2018: Journal of Applied Microbiology
Wei Dong, Peter Setlow
AIMS: To investigate effects of fluoride ion (F- ) on, and kinetics of its movement into and out of, spores and growing cells of Bacillus species. METHODS AND RESULTS: Effects of F- on Bacillus cell growth, spore germination and outgrowth and heat resistance were investigated, as well as F- movement into and out of spores using 19 F NMR. F- inhibited Bacillus subtilis spore germination and outgrowth, and YhdU, now named FluC, was crucial to prevent F- accumulation in growing cells and to minimize F- inhibition of spore germination...
November 14, 2018: Journal of Applied Microbiology
Laurent Simon Tiemele Amoikon, Francine Moussan Aké, Théodore N'Dédé Djéni, Cécile Grondin, Serge Casaregola, Koffi Marcellin Djè
AIMS: To investigate the genotypic diversity and enzymatic activity of yeast flora isolated from spontaneous fermenting saps of warious palm trees (Borassus aethiopum, Raphia hookeri, Elaeis guineensis) tapped for palm wines. METHODS AND RESULTS: PCR-RFLP of ITS-5.8S rDNA combined to 26S rRNA gene and/or the partial ACT1 gene sequencing were applied for yeast characterization, and their enzymatic profiles assessed by using API ZYM kits. Thirteen genera and 23 species were identified, with the highest diversity (14 species) in raffia wine...
November 12, 2018: Journal of Applied Microbiology
Maria Alexandra Cucu, Giovanna Gilardi, Massimo Pugliese, Slavica Matic, Ulrich Gisi, Maria Lodovica Gullino, Angelo Garibaldi
AIMS: The response of rhizosphere and bulk soil indigenous microbial communities focusing on nitrifiers was evaluated after the application of different biological control agents (BCAs) (Bacillus, Trichoderma, Pseudomonas) and compost in controlling lettuce Fusarium wilt. METHODS AND RESULTS: Experiments were conducted "in situ" over two lettuce cropping seasons. Total fungal, bacterial and archaeal populations and the nitrifiers were analysed using quantitative polymerase chain reaction (qPCR) method...
November 11, 2018: Journal of Applied Microbiology
Niloufar Roshan, Thomas V Riley, Daniel R Knight, James H Steer, Katherine A Hammer
AIMS: To investigate the mechanisms of action of natural products with bactericidal (cinnamon root powder, peppermint oil, trans-cinnamaldehyde, menthol and zingerone) or bacteriostatic (fresh garlic bulb extract, garlic clove powder, Leptospermum honey and allicin) activity against two C. difficile strains. METHODS AND RESULTS: Bactericidal products significantly reduced intracellular ATP after 1 h (P ≤ 0.01), quantified using the BacTiter-Glo reagent, and damaged the cell membrane, shown by the leakage of both 260-nm absorbing materials and protein, and the uptake of propidium iodide...
November 9, 2018: Journal of Applied Microbiology
Wafa Talbi, Tesnime Ghazouani, Daniela Braconi, Rim Ben Abdallah, Faten Raboudi, Annalisa Santucci, Sami Fattouch
AIM: To clarify the effects of selenium, parameters related to oxidative issues, as well as the antioxidant response were investigated on an autochthonous wine yeast strain. METHODS AND RESULTS: Antioxidant enzyme activity, Gel electrophoresis, Western Blot and MDA level were used to investigate the effects of different concentration of selenium in wine yeast. We found that selenium is able to affect the enzymatic activities of catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD)...
November 8, 2018: Journal of Applied Microbiology
Xue Shen, Houru Liu, Gen Li, Xuming Deng, Jianfeng Wang
AIMS: To determine the anti-virulence properties of silibinin against suilysin (SLY), a virulence factor of Streptococcus suis serotype 2 (SS2) that plays an important role in the pathogenesis of S. suis infection and its protective effect against SS2 infection in a mouse model. METHODS AND RESULTS: Susceptibility testing, hemolysis assay and Western blot assays were employed to evaluate the performance of silibinin on SLY pore-forming activity. Cytotoxicity assays and mouse infection tests were also performed to determine the efficacy of silibinin against SS2 infection...
November 8, 2018: Journal of Applied Microbiology
Karin A Nyberg, Gunnar M Andersson, Josefine Elving
AIM: To investigate the survival of E. coli O157:H7 and Salmonella Typhimurium in cowpats on pasture in a temperate Nordic climate. METHODS AND RESULTS: The study consists of two parts, the first part using artificially created cowpats inoculated with E. coli O157:H7 and Salmonella Typhimurium and the second part using cowpats from empty pastures on which cattle herds positive for E. coli O157:H7 had grazed six month previously. Artificial cowpats were created, placed in an outdoor field station in June, August and October, and sampled over one year...
November 5, 2018: Journal of Applied Microbiology
Dorothee Janßen, Matthias A Ehrmann, Rudi F Vogel
In a previous study we used a 5-day fermenting sausage model to characterize assertiveness of Lactobacillus curvatus and L. sakei starter strains towards employ autochthonous contaminants. In this work we probed those findings and their transferability to real sausage fermentation including the drying process in an industrial ring trial experiment METHODS AND RESULTS: Raw fermented sausages ("salami") were produced with 3 L. curvatus and 4 L. sakei strains as starter cultures in cooperation with three manufacturers from Germany...
November 1, 2018: Journal of Applied Microbiology
Ross C Beier, Roger B Harvey, Toni L Poole, Michael E Hume, Tawni L Crippen, Linda D Highfield, Walid Q Alali, Kathleen Andrews, Robin C Anderson, David J Nisbet
AIMS: Investigate the interactions of organic acids (OAs), acetic, butyric, citric, formic, lactic and propionic acid against 50 Gram-positive VRE strains to determine whether pH, undissociated or dissociated acid forms correlate with bacterial inhibition. METHODS AND RESULTS: Concentrations of undissociated and dissociated OAs at the molar MICs (MICM s) of the VRE were calculated using the Henderson-Hasselbalch equation. The pH at the MICM s of all VRE strains against acetic, butyric, formic and propionic acids was similar, 4...
November 1, 2018: Journal of Applied Microbiology
Siran Wang, Zhihao Dong, Junfeng Li, Lei Chen, Tao Shao
AIMS: To examine the characteristics of three isolated Pediococcus acidilactici strains (LTG7, LOG9 and LH9) and evaluate their effects on silage quality, nutritive value and in vitro ruminal digestibility in a variety of forages. METHODS AND RESULTS: One commercial inoculant Lactobacillus plantarum MTD-1 (G) and three isolated lactic acid bacteria (LAB) strains were measured by morphological, physiological and biochemical tests. All the LAB strains were added to Italian ryegrass (Lolium multiflorum Lam...
November 1, 2018: Journal of Applied Microbiology
M Ganuza, N Pastor, M Boccolini, J Erazo, S Palacios, C Oddino, M M Reynoso, M Rovera, A M Torres
AIM: to investigate the impact of inoculating peanut seeds with the biocontrol agent Trichoderma harzianum ITEM 3636 on the structure of bacterial and fungal communities from agricultural soils. METHODS AND RESULTS: PCR-Denaturing gradient gel electrophoresis (PCR-DGGE) and next-generation sequencing (NGS) of amplicons (or marker gene amplification metagenomics) were performed to investigate potential changes in the structure of microbial communities from fields located in a peanut-producing area in the province of Córdoba, Argentina...
November 1, 2018: Journal of Applied Microbiology
C Vipin, M Mujeeburahiman, A B Arun, P Ashwini, Suryavanshi V Mangesh, P D Rekha
AIM: The aim of this study was to investigate the natural variation in the antibiotic sensitivity, biofilm formation and virulence among Pseudomonas aeruginosa isolated from the catheter-associated urinary tract infection (CAUTI) from a single centre. METHODS AND RESULTS: P. aeruginosa strains were isolated from the patients with CAUTI after obtaining informed consent. These isolates were identified by routine biochemical methods and 16S rRNA gene sequencing. Antibiotic sensitivity and virulence factors were measured using standard protocols...
October 29, 2018: Journal of Applied Microbiology
So-Yeon Jeong, Tae Gwan Kim
AIMS: Microbial consortia can be more efficient at biological processes than single isolates. The purposes of this study were to design and evaluate a synthetic microbial consortium containing the methanotroph Methylocystis sp. M6 and the helper Hyphomicrobium sp. NM3, and develop a novel methanotrophic process for this consortium utilizing a dialysis membrane. METHODS AND RESULTS: Hyphomicrobium increased the methane-oxidation rate (MOR), biomass, and stability at a dilution rate of 0·067·d-1 in fed-batch co-culture...
October 26, 2018: Journal of Applied Microbiology
Wenshu Lin, Dan Yuan, Zhirui Deng, Bing Niu, Qin Chen
OBJECTIVES: In our previous research, we have developed a new combination disinfectant, glutaraldehyde-didecyldimethylammonium bromide (GD). It was verified that GD had a strong effect on both Escherichia coli and Staphylococcus aureus. In this work, Candida albicans (C. albicans) was selected as an object, and it could be killed by GD. We aimed to investigate the cellular and molecular mechanism of GD effecting on C. albicans. METHODS AND RESULTS: The results of sterilization experiment indicated that GD was effective on C...
October 26, 2018: Journal of Applied Microbiology
Anou M Somboro, John Osei Sekyere, Daniel G Amoako, Hezekiel M Kumalo, René Khan, Linda A Bester, Sabiha Y Essack
AIMS: We hypothesised and confirmed that tannic acid (TA) reverses carbapenem resistance by inhibiting carbapenemases in class A and B carbapenemase-producing Enterobacteriaceae. METHODS AND RESULTS: MICs of carbapenems in the presence and absence of TA and other efflux pump inhibitors, TA-carbapenemases inhibition assays and computational studies showed that TA had the greatest effect on metallo-β-lactamases (MBLs) followed by class A serine-β-lactamases (SBLs)...
October 26, 2018: Journal of Applied Microbiology
Guillaume Larrivière-Gauthier, Alexandre Thibodeau, Ann Letellier, Étienne Yergeau, Philippe Fravalo
AIM: Observe the transfer of the digestive microbiota from sow to piglet, describe the impact of the sow's Salmonella shedding on this transfer and identify transferred populations that could be associated with the future Salmonella status of the piglets. METHODS AND RESULTS: Salmonella shedding status of 19 sows was determined at the beginning and end of gestation. Four piglets were randomly selected from each sow. Using MiSeq, the microbiotas of the sows at the end of gestation and of their piglets 1 day before weaning were described...
October 26, 2018: Journal of Applied Microbiology
Hoa Thi Nguyen, Soonok Kim, Nan Hee Yu, Ae Ran Park, Hyeokjun Yoon, Chang-Hwan Bae, Joo Hong Yeo, In Seon Kim, Jin-Cheol Kim
AIMS: To evaluate the antimicrobial activities of an active compound isolated from the culture broth of Amphirosellinia nigrospora JS-1675 against various plant pathogenic bacteria and fungi. METHODS AND RESULTS: While screening for bioactive secondary metabolites from endophytic fungi, we found that Amphirosellinia nigrospora JS-1675 showed strong in vitro antibacterial activity against Ralstonia solanacearum. One compound (1) was isolated and identified as (4S, 5S, 6S)-5, 6-epoxy-4-hydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one...
October 25, 2018: Journal of Applied Microbiology
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