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Investigation of the Best Saccharomyces cerevisiae Growth Condition.
Electronic Physician 2017 January
INTRODUCTION: Saccharomyces cerevisiae is known as one of the useful yeasts which are utilized in baking and other industries. It can be easily cultured at an economic price. Today the introduction of safe and efficient carriers is being considered. Due to its generally round shape, and the volume that is enclosed by its membrane and cell wall, it is used to encapsulate active materials to protect them from degradation or to introduce a sustained release drug delivery system. Providing the best conditions in order to achieve the best morphological properties of Saccharomyces cerevisiae as a carrier.
METHODS: In this research, the most suitable growth condition of yeast cells which provides the best size for use as drug carriers was found by a bioreactor in a synthetic culture medium. Yeast cell reproduction and growth curves were obtained, based on pour plate colony counting data and UV/Visible sample absorption at 600 nm. Yeast cell growth patterns and growth rates were determined by Matlab mathematical software.
RESULTS: Results showed that pH=4 and dissolving oxygen (DO) 5% was the best condition for yeast cells to grow and reproduce. This condition also provided the largest size (2 × 3 μ) yeast cells.
CONCLUSION: Owing to the yeast cells' low-cost production and their structural characteristics, they could be used as potent drug carriers.
FUNDING: This work was supported by a grant from the Vice Chancellor of Research of Mashhad University of Medical Sciences.
METHODS: In this research, the most suitable growth condition of yeast cells which provides the best size for use as drug carriers was found by a bioreactor in a synthetic culture medium. Yeast cell reproduction and growth curves were obtained, based on pour plate colony counting data and UV/Visible sample absorption at 600 nm. Yeast cell growth patterns and growth rates were determined by Matlab mathematical software.
RESULTS: Results showed that pH=4 and dissolving oxygen (DO) 5% was the best condition for yeast cells to grow and reproduce. This condition also provided the largest size (2 × 3 μ) yeast cells.
CONCLUSION: Owing to the yeast cells' low-cost production and their structural characteristics, they could be used as potent drug carriers.
FUNDING: This work was supported by a grant from the Vice Chancellor of Research of Mashhad University of Medical Sciences.
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