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Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor.
Analyst 2017 Februrary 15
Herein a convenient strategy was developed to quantify protein SUMOylation. The amount of target proteins and SUMO proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the SUMOylation degree of the target proteins.
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