Comparative, Histological and Histomorphometric Analysis of Three Anorganic Bovine Xenogenous Bone Substitutes: Bio-Oss, Bone-Fill and Gen-Ox Anorganic.

INTRODUCTION: Anorganic bovine xenogenous grafts show the best performance as bone substitutes in implantodontics. Bio-Oss is the world's most widely used and investigated anorganic bone substitute. This article compares two anorganic bovine bone substitutes (Bone-Fill and Gen-Ox anorganic) with Bio-Oss.

MATERIALS AND METHODS: Eight New Zealand rabbits were implanted with 4 titanium cylinders randomly filled with Bio-Oss, Bone-Fill, Gen-Ox anorganic or a blood clot. Four animals were sacrificed after 8 weeks; 12 weeks later, the remaining four were sacrificed. The contents of the cylinders were removed, cut and stained with HE before they were evaluated with an optical microscope. The samples were submitted to histomorphometry for analysis.

RESULTS: The bone formation with Bio-Oss at 8 weeks was 8.43 mm(2); at 12 weeks, it was 9.32 mm(2). The bone formation with Bone-Fill at 8 weeks was 7.24 mm(2); at 12 weeks, it was 9.01 mm(2). The bone formation with Gen-Ox anorganic at 8 weeks was 2.78 mm(2); at 12 weeks, it was 3.02 mm(2). The bone formation with the blood clot at 8 weeks was 0.65 mm(2); at 12 weeks, it was 0.63 mm(2).

CONCLUSION: Following this model, Bone-Fill was comparable to Bio-Oss and superior to Gen-Ox and blood clot.