[Effects of mesenchymal stem cells on angiogenesis of cervical cancer HeLa cancer cell line HeLa in vivo].

OBJECTIVE: To explore the effects of bone marrow-derived mesenchymal stem cell (MSC) on angiogenesis of cervical cancer cell.

METHODS: MSCs from umbilical cord were mixed with cervical cancer cell line HeLa cells and subcutaneously plated in armpit area of nude mice to examine the in vivo tumor growth. Micro-vessel density (MVD) in tumor tissue was examined by CD34 immunohistochemical staining. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR) were employed to detect the expression of proangiogenesis factors.

RESULTS: MSC could effectively promote the cervical cancer growth in vivo as compared with controls (P<0.05). The immunohistochemical results of CD34 staining showed that MVD of cervical cancer was higher in MSC group than that in control group (P<0.05). And there was a higher expression of vascular endothelial growth factor (VEGF) in MSC than controls.

CONCLUSION: MSC promotes the in vivo angiogenesis of cervical cancer cell line by VEGF production.