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Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed gamma-glutamylmethylamide synthetase and dried yeast cells.
Bioscience, Biotechnology, and Biochemistry 2009 December
Gamma-glutamylmethylamide synthetase and dried baker's yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mM theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD(+).
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