Md Badrul Alam, Marufa Naznin, Syful Islam, Fanar Hamad Alshammari, Hee-Jeong Choi, Bo-Rim Song, Sunghwan Kim, Sang-Han Lee
The secondary metabolites profiling of Nymphaea nouchali stem (NNSE) extract was carried out using a high-resolution mass spectroscopic technique. The antioxidant effects of NNSE, as well as the underlying mechanisms, were also investigated in tert-butyl hydroperoxide ( t -BHP)-stimulated oxidative stress in RAW264.7 cells. Tandem mass spectroscopy with (-) negative mode tentatively revealed the presence of 54 secondary metabolites in NNSE. Among them, phenolic acids and flavonoids were predominant. Phenolic acids (brevifolincarboxylic acid, p -coumaroyltartaric acid, niazinin B, lalioside, 3-feruloylquinic acid, and gallic acid- O -rutinoside), flavonoids (elephantorrhizol, apigenin-6-C-galactoside 8-C-arabinoside, and vicenin-2), sialic acid (2-deoxy-2,3-dehydro-N-acetylneuraminic acid), and terpenoid (α-γ-onoceradienedione) were identified in NNSE for the first time...
May 3, 2021: Antioxidants (Basel, Switzerland)