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https://www.readbyqxmd.com/read/27882147/molecular-profiling-of-a-simple-rat-model-of-open-tibial-fractures-with-hematoma-and-periosteum-disruption
#1
Jose Rafael Villafan-Bernal, Lorenzo Franco-De La Torre, Ana Soledad Sandoval-Rodriguez, Juan Armendariz-Borunda, Juan Luis Alcala-Zermeno, Jose Alfonso Cruz-Ramos, Gabriela Lopez-Armas, Blanca Estela Ramirez-Bastidas, Gracia Viviana González-Enríquez, Emerson Armando Collazo-Guzman, Raigam Jafet Martinez-Portilla, Sergio Sánchez-Enríquez
Bone fractures are a worldwide public health concern. Therefore, improving understanding of the bone healing process at a molecular level, which could lead to the discovery of potential therapeutic targets, is important. In the present study, a model of open tibial fractures with hematoma disruption, periosteal rupture and internal fixation in 6-month-old male Wistar rats was established, in order to identify expression patterns of key genes and their protein products throughout the bone healing process. A tibial shaft fracture was produced using the three-point bending technique, the hematoma was drained through a 4-mm incision on the medial aspect of the tibia and the fracture stabilized by inserting a needle into the medullary canal...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27864295/endothelial-cells-produce-bone-morphogenetic-protein-6-required-for-iron-homeostasis-in-mice
#2
Susanna Canali, Kimberly B Zumbrennen-Bullough, Amanda B Core, Chia-Yu Wang, Manfred Nairz, Richard Bouley, Filip K Swirski, Jodie L Babitt
Bone morphogenetic protein (BMP)6 signaling in hepatocytes is a central transcriptional regulator of the iron hormone hepcidin to control systemic iron balance. How iron levels are sensed to regulate hepcidin production is not known, but local induction of liver BMP6 expression by iron is proposed to have a critical role. To identify the cellular source of BMP6 responsible for hepcidin and iron homeostasis regulation, we generated mice with tissue specific ablation of Bmp6 in different liver cell populations and evaluated their iron phenotype...
November 18, 2016: Blood
https://www.readbyqxmd.com/read/27842921/suppression-of-osteoblast-related-genes-during-osteogenic-differentiation-of-adipose-tissue-derived-stromal-cells
#3
Yahya Açil, Amir Alexander Ghoniem, Aydin Gülses, Tobias Kisch, Felix Stang, Jörg Wiltfang, Matthias Gierloff
Recent studies indicated a lower osteogenic differentiation potential of adipose tissue-derived stromal cells (ASCs) compared to bone marrow derived mesenchymal stromal cells. The aim of this study was to evaluate the effects of potent combinations of highly osteogenic bone morphogenetic proteins (BMPs) in order to enhance the osteogenic differentiation potential of ASCs. Human ASCs were cultured for 10 days in the presence of osteogenic medium consisting of dexamethasone, ß-glycerophosphate and ascorbat-2-phosphate (OM) supplemented with BMP-2, BMP-6, BMP-9+IGF-2 and BMP-2,-6,-9 (day 1+2: 50 ng/ml, days 3-6: 100 ng/ml, days 7-10: 200 ng/ml)...
October 18, 2016: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/27056281/bmp-9-dependent-pathways-required-for-the-chondrogenic-differentiation-of-pluripotent-stem-cells
#4
Alan Cheng, Amber Renee Gustafson, Christine Elizabeth Schaner Tooley, Mei Zhang
Current cartilage repair therapies focus on the delivery of chondrocytes differentiated from mesenchymal stem cells, and thus understanding the factors that promote chondrogenesis may lead to improved therapies. Several bone morphogenetic proteins (BMPs) have been implicated in chondrogenic differentiation and/or chondrocyte function. Although the signaling pathways downstream of BMPs have been studied in other systems, their role in chondrogenesis is less well characterized. Here, we investigated the effects of BMP-9 in chondroprogenitor cells...
December 2016: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/26802359/new-ligand-binding-function-of-human-cerberus-and-role-of-proteolytic-processing-in-regulating-ligand-receptor-interactions-and-antagonist-activity
#5
Senem Aykul, Erik Martinez-Hackert
Cerberus is a key regulator of vertebrate embryogenesis. Its biological function has been studied extensively in frog and mouse embryos. Its ability to bind and antagonize the transforming growth factor-β (TGF-β) family ligand Nodal is well established. Strikingly, the molecular function of Cerberus remains poorly understood. The underlying reason is that Cerberus is a complex, multifunctional protein: It binds and inhibits multiple TGF-β family ligands, it may bind and inhibit some Wnt family members, and two different forms with distinct activities have been described...
February 13, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/26781228/thymic-carcinoma-with-cartilage-formation-in-a-dog
#6
J K Chambers, Y Yoshino, R Fujiwara, R Nishimura, H Nakayama, K Uchida
An 11-year-old female Chihuahua exhibited respiratory distress and a computed tomography scan showed a large mass in the anterior thoracic cavity. During surgery, it was found that the mass was strongly adherent to surrounding tissue. A histopathological examination of a biopsy sample from the mass revealed proliferation of atypical epithelial cells and cartilage formation admixed with mature lymphocytes. Immunohistochemically, the tumour cells, as well as the normal canine thymic epithelial cells, were positive for pan-cytokeratin (CK), CK5/6, CK19, p63 and bone morphogenetic protein (BMP) 6...
January 2016: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/26779985/bmp-6-modulates-somatostatin-effects-on-luteinizing-hormone-production-by-gonadrotrope-cells
#7
Kishio Toma, Fumio Otsuka, Kohei Oguni, Tomohiro Terasaka, Motoshi Komatsubara, Naoko Tsukamoto-Yamauchi, Kenichi Inagaki, Hirofumi Makino
The effects of somatostatin analogs and roles of BMP-6 in the regulation of luteinizing hormone (LH) secretion were investigated using mouse gonadotrope LβT2 cells. LH mRNA expression and LH secretion induced by GnRH were suppressed by treatments with somatostatin analogs, including octreotide and pasireotide, in LβT2 cells. Of note, the inhibitory effects of somatostatin analogs on LH secretion were enhanced by the action of BMP-6. BMP-6 increased the expression levels of somatostatin receptor (SSTR)5, suggesting that BMP-6 upregulates SSTR activity that leads to reduction of GnRH-induced LH secretion...
February 2016: Peptides
https://www.readbyqxmd.com/read/26751737/bmp-6-inhibits-the-metastasis-of-mda-mb-231-breast-cancer-cells-by-regulating-mmp-1-expression
#8
Fen Hu, Yunfeng Zhang, Mi Li, Lina Zhao, Jing Chen, Shuang Yang, Xiujun Zhang
Bone morphogenetic protein-6 (BMP-6) is a multifunctional molecule with distinct abilities in embryogenesis and organogenesis. In the present study, our results showed that the rate of BMP-6-negative expression was 30.56% in breast cancer tissues, but was 9.58% in normal tissues by immunohistochemical staining. This implied that BMP-6 expression is absent in breast cancer tissues and may suppress breast cancer metastasis. In addition, stable overexpression of BMP-6 in MDA-MB‑231 cells was established to analyze the metastatic ability...
March 2016: Oncology Reports
https://www.readbyqxmd.com/read/26728496/the-combined-mechanism-of-bone-morphogenetic-protein-and-calcium-phosphate-induced-skeletal-tissue-formation-by-human-periosteum-derived-cells
#9
J Bolander, W Ji, L Geris, V Bloemen, Y C Chai, J Schrooten, F P Luyten
When combining osteogenic progenitor cells such as human periosteum derived cells (hPDCs) with osteoconductive biomaterials like calcium phosphate (CaP)-scaffolds, in vivo bone formation can be achieved. This process is dependent on the early activation of Bone morphogenetic protein (BMP)-signalling. However, the bone forming process is slow and routinely only a limited amount of bone and bone marrow is formed. Therefore, we hypothesised that a robust clinically relevant outcome could be achieved by adding more physiological levels of potent BMP-ligands to these cell- and CaP-based constructs...
January 5, 2016: European Cells & Materials
https://www.readbyqxmd.com/read/26700847/accelerated-chondrogenic-differentiation-of-human-perivascular-stem-cells-with-nell-1
#10
Chen-Shuang Li, Xinli Zhang, Bruno Péault, Jie Jiang, Kang Ting, Chia Soo, Yan-Heng Zhou
Osteoarthritis is the leading cause of disability in the US. Consequently, there is a pressing need for restoring the structural and functional properties of diseased articular cartilage. Yet the search for the right combination of proper target cells and growth factors for cartilage regeneration remains challenging. In this study, we first tested the intrinsic chondrogenic differentiation ability of human perivascular stem cells (hPSCs), a novel source of mesenchymal stem cells (MSCs) isolated by fluorescence-activated cell sorting (FACS) from human adipose tissue...
February 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/26676858/combined-delivery-of-pdgf-bb-and-bmp-6-for-enhanced-osteoblastic-differentiation
#11
T Tolga Demirtaş, Eda Göz, Ayşe Karakeçili, Menemşe Gümüşderelioğlu
Natural microenvironment during bone tissue regeneration involves integration of multiple biological growth factors which regulate mitogenic activities and differentiation to induce bone repair. Among them platelet derived growth factor (PDGF-BB) and bone morphogenic protein-6 (BMP-6) are known to play a prominent role. The aim of this study was to investigate the benefits of combined delivery of PDGF-BB and BMP-6 on proliferation and osteoblastic differentiation of MC3T3-E1 preosteoblastic cells. PDGF-BB and BMP-6 were loaded in gelatin and poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) particles, respectively...
January 2016: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/26660854/bone-morphogenetic-protein-6-bmp-6-stimulates-the-antrum-formation-by-the-regulation-of-its-signalling-pathway-in-caprine-pre-antral-follicles-cultured-in-vitro
#12
V R Araújo, G M Silva, A B G Duarte, D M Magalhães-Padilha, A P Almeida, F O Lunardi, M K B Serafim, A A A Moura, C C Campello, A P R Rodrigues, J R Figueiredo
BMP-6 has been found to be important to ovarian cells and oocyte, as well as to uterus. Thus, this study investigated the effect of bone morphogenetic protein (BMP-6) and recombinant follicle-stimulating hormone (rFSH) alone or in combination on the in vitro culture (IVC) of isolated caprine secondary follicles (Experiment 1) and the mRNA levels for BMP receptors/Smad signalling pathway (BMPR1A, BMPR2, SMAD1, SMAD4, SMAD5, SMAD6, SMAD7 and SMAD8) in vivo and in vitro using BMP-6 (Experiment 2). Secondary follicles were cultured in αMEM(+) alone (control medium) or supplemented with BMP-6 at 1 or 10 ng/ml and rFSH alone or the combination of both BMP-6 concentrations and rFSH...
February 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/26636769/upregulation-of-bone-morphogenetic-protein-2-synthesis-and-consequent-collagen-ii-expression-in-leptin-stimulated-human-chondrocytes
#13
Shun-Fu Chang, Rong-Ze Hsieh, Kuo-Chin Huang, Cheng Allen Chang, Fang-Yao Chiu, Hsing-Chun Kuo, Cheng-Nan Chen, Yu-Ping Su
Bone morphogenetic proteins (BMPs) play positive roles in cartilage development, but they can barely be detected in healthy articular cartilage. However, recent evidence has indicated that BMPs could be detected in osteoarthritic and damaged cartilage and their precise roles have not been well defined. Extremely high amounts of leptin have been reported in obese individuals, which can be associated with osteoarthritis (OA) development. The aim of this study was to investigate whether BMPs could be induced in human primary chondrocytes during leptin-stimulated OA development and the underlying mechanism...
2015: PloS One
https://www.readbyqxmd.com/read/26475719/poly-lactic-co-glycolide-polymer-constructs-cross-linked-with-human-bmp-6-and-vegf-protein-significantly-enhance-rat-mandible-defect-repair
#14
Anusuya Das, Brian A Fishero, J Jared Christophel, Ching-Ju Li, Nikita Kohli, Yong Lin, Abhijit S Dighe, Quanjun Cui
We have previously shown that the combined delivery of mesenchymal stem cells (MSCs), vascular endothelial growth factor (VEGF) and bone morphogenetic protein 6 (BMP-6) induces significantly more bone formation than that induced by the delivery of any single factor or a combination of any two factors. We now determine whether the exogenous addition of VEGF and BMP-6 is sufficient for bone healing when MSCs are not provided. Poly(lactic-co-glycolic acid) (PLAGA) microsphere-based three-dimensional scaffolds (P) were fabricated by thermal sintering of PLAGA microspheres...
April 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/26166168/characterization-of-transforming-growth-factor-beta-superfamily-growth-factors-transcriptional-factors-and-lipopolysaccharide-in-bovine-cystic-ovarian-follicles
#15
I M Polat, E Alçiğir, M Pekcan, S A Vural, E Özenç, H E Canatan, Ş Küplülü, G E Dal, M O Yazlik, C Baklaci, M R Vural
The process of transformation of growing bovine follicles into cysts is still a mystery. Local expression of proteins or factors, including transforming growth factor β, growth factors, and transcription factors, plays a central role in mammals. Therefore, in abattoir-derived cystic ovarian follicles and follicular fluid, the role of some transforming growth factor β superfamily proteins, insulinlike growth factor-1 (IGF-1) and GATA-4 and GATA-6, were investigated. The relationship between intrafollicular lipopolysaccharide (LPS) and etiopathogenesis of ovarian cysts was also assessed...
October 1, 2015: Theriogenology
https://www.readbyqxmd.com/read/26081853/-delta12-prostaglandinj2-nano-capsule-up-regulates-growth-factor-expression-and-enhances-bone-regeneration-in-rats
#16
Lili Chen, Fen Wei, Weilian Sun, Peihui Ding, Xiaotao Chen, Yanmin Wu
OBJECTIVE: To investigate the effect of local delivery of delta12-prostaglandinJ2-loaded poly (lactic-co-glycolic acid) (Δ(12)-PGJ2-NC) on growth factors expression and bone formation. METHODS: Δ(12)-PGJ2-NC was prepared by the emulsion solvent diffusion method. The physical and chemical properties of the nanoparticles were evaluated by particle size analysis, transmission electron microscopy, drug-loading ratio and the in vitro release study. Then standardized transcortical defect (5...
March 2015: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/26047946/activin-a-inhibits-bmp-signaling-by-binding-acvr2a-and-acvr2b
#17
Oddrun Elise Olsen, Karin Fahl Wader, Hanne Hella, Anne Kærsgaard Mylin, Ingemar Turesson, Ingerid Nesthus, Anders Waage, Anders Sundan, Toril Holien
BACKGROUND: Activins are members of the TGF-β family of ligands that have multiple biological functions in embryonic stem cells as well as in differentiated tissue. Serum levels of activin A were found to be elevated in pathological conditions such as cachexia, osteoporosis and cancer. Signaling by activin A through canonical ALK4-ACVR2 receptor complexes activates the transcription factors SMAD2 and SMAD3. Activin A has a strong affinity to type 2 receptors, a feature that they share with some of the bone morphogenetic proteins (BMPs)...
2015: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/26037311/gene-profile-of-soluble-growth-factors-involved-in-angiogenesis-in-an-adipose-derived-stromal-cell-endothelial-cell-co-culture-3d-gel-model
#18
Xiangzhu Cun, Jing Xie, Shiyu Lin, Na Fu, Shuwen Deng, Qiang Xie, Juan Zhong, Yunfeng Lin
OBJECTIVES: The aim of this study was to investigate gene expressions of growth factors for angiogenesis, in a three-dimensional (3D) gel populated with adipose-derived stromal cells (ASCs) and endothelial cells (ECs) in co-culture. MATERIALS AND METHODS: The 3D gel, mixed with green fluorescent protein (GFP)-positive ASCs and DsRed-Express-positive ECs, 1:1 ratio, was established in vitro. The phenomenon of angiogenesis was observed using confocal microscopy. To detect gene expressions for growth factor proteins in both ASCs and ECs, transwell co-culture was used, and cell lysate samples were collected at 1, 3, 5 and 7 days...
August 2015: Cell Proliferation
https://www.readbyqxmd.com/read/26019649/effect-of-bmp-6-on-development-and-maturation-of-mouse-preantral-follicles-in-vitro
#19
Xiyan Wang, Li Su, Xiaoyan Pan, Jian Yao, Zhixin Li, Xuenan Wang, Bangsheng Xu
The aim of this study was to investigate the effect and mechanism of bone morphogenetic protein-6 (BMP-6) on the growth and maturation of mouse follicles in vitro. Preantral follicles isolated from mice were incubated with recombinant human BMP-6 (rhBMP-6) before analysis. BMP-6 expression was detected by immunofluorescence and western blot. Maturation of oocytes was observed microscopically. Estradiol (E2) and progesterone (P4) levels were measured by enzyme-linked immunosorbent assay. Expression of steroidogenesis-related genes was detected by reverse transcription quantitative polymerase chain reaction...
March 4, 2015: Biotechnology, Biotechnological Equipment
https://www.readbyqxmd.com/read/25902833/admixture-mapping-of-coronary-artery-calcification-in-african-americans-from-the-nhlbi-family-heart-study
#20
Felicia Gomez, Lihua Wang, Haley Abel, Qunyuan Zhang, Michael A Province, Ingrid B Borecki
BACKGROUND: Coronary artery calcification (CAC) is an imaging biomarker of coronary atherosclerosis. In European Americans, genome-wide association studies (GWAS) have identified several regions associated with coronary artery disease. However, few large studies have been conducted in African Americans. The largest meta-analysis of CAC in African Americans failed to identify genome-wide significant variants despite being powered to detect effects comparable to effects identified in European Americans...
2015: BMC Genetics
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