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https://www.readbyqxmd.com/read/29789443/low-intensity-pulsed-ultrasound-stimulation-facilitates-in-vitro-osteogenic-differentiation-of-human-adipose-derived-stem-cells-via-up-regulation-of-heat-shock-protein-hsp-70-hsp90-and-bone-morphogenetic-protein-bmp-signaling-pathway
#1
Zhonglei Zhang, Yalin Ma, Shaowen Guo, Yi He, Gang Bai, Wenjun Zhang
Low-intensity pulsed ultrasound (LIPUS) has positive effects on osteogenic differentiation. However, the effect of LIPUS on osteogenic differentiation of human adipose-derived stem cells (hASCs) is unclear. In the present study, we investigated whether LIPUS could promote the proliferation and osteogenic differentiation of hASCs. hASCs were isolated and osteogenically induced with LIPUS stimulation at 20 and 30 mW cm-2 for 30 min day-1 Cell proliferation and osteogenic differentiation potential of hASCs were respectively analyzed by cell counting kit-8 assay, Alizarin Red S staining, real-time polymerase chain reaction, and Western blotting...
May 29, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29784586/ultrasound-mediated-gene-delivery-enhances-tendon-allograft-integration-in-mini-pig-ligament-reconstruction
#2
Maxim Bez, Thomas J Kremen, Wafa Tawackoli, Pablo Avalos, Dmitriy Sheyn, Galina Shapiro, Joseph C Giaconi, Shiran Ben David, Jess G Snedeker, Zulma Gazit, Katherine W Ferrara, Dan Gazit, Gadi Pelled
Ligament injuries occur frequently, substantially hindering routine daily activities and sports participation in patients. Surgical reconstruction using autogenous or allogeneic tissues is the gold standard treatment for ligament injuries. Although surgeons routinely perform ligament reconstructions, the integrity of these reconstructions largely depends on adequate biological healing of the interface between the ligament graft and the bone. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would lead to significantly improved ligament graft integration...
April 26, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29767257/bmp%C3%A2-6-protects-retinal-pigment-epithelial-cells-from-oxidative-stress%C3%A2-induced-injury-by-inhibiting-the-mapk-signaling-pathways
#3
Li Chen, Ming Liu, Yan Luan, Yingfei Liu, Zhichao Zhang, Bo Ma, Xuan Liu, Yong Liu
Worldwide, neovascular age‑related macular degeneration (nAMD) is one of the most common causes of blindness in the elderly. In particular, degeneration of retinal pigment epithelial (RPE) cells represents the main pathological process in the development of nAMD, and oxidative stress serves a major role. The present study aimed to investigate the association between bone morphogenetic protein 6 (BMP‑6) and nAMD. BMP‑6 concentration was significantly reduced in patients with wet nAMD compared with in the control group...
May 14, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29573911/limonene-promotes-osteoblast-differentiation-and-2-deoxy-d-glucose-uptake-through-p38mapk-and-akt-signaling-pathways-in-c2c12-skeletal-muscle-cells
#4
Ilavenil Soundharrajan, Da Hye Kim, Srigopalram Srisesharam, Palaniselvam Kuppusamy, Ravikumar Sivanesan, Ki Choon Choi
BACKGROUND: Limonene is a cyclic monoterpene (CTL) found in citrus fruits and many plant kingdoms. It has attracted attention as potential molecule due to its diverse biological activities. However, molecular mechanism involved in the osteogenic induction of CTL in C2C12 skeletal muscle cells remain unclear. PURPOSE: Skeletal development maintains the bone homeostasis through bone remodeling process. It coordinated between the osteoblast and osteoblast process. Osteoporosis is one of the most common bone diseases caused by a systemic reduction in bone mass...
March 12, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29545164/interaction-between-orexin-a-and-bone-morphogenetic-protein-system-on-progesterone-biosynthesis-by-rat-granulosa-cells
#5
Shiho Fujita, Toru Hasegawa, Yuki Nishiyama, Satoshi Fujisawa, Yasuhiro Nakano, Takahiro Nada, Nahoko Iwata, Yasuhiko Kamada, Hisashi Masuyama, Fumio Otsuka
The involvement of orexins in reproductive function has been gradually uncovered. However, the functional role of orexins in ovarian steroidogenesis remains unclear. In the present study, we investigated the effects of orexin A on ovarian steroidogenesis by using rat primary granulosa cells that express both OX1 and OX2 receptors for orexins. Treatment with orexin A enhanced progesterone, but not estradiol, biosynthesis induced by FSH, whereas it did not affect basal levels of progesterone or estradiol. In accordance with the effects on steroidogenesis, orexin A increased the mRNA levels of progesterogenic enzymes, including StAR, P450scc and 3βHSD, but not P450arom, and cellular cAMP synthesis induced by FSH...
March 12, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29544628/interaction-of-melatonin-and-bmp-6-in-ovarian-steroidogenesis
#6
Fumio Otsuka
The bone morphogenetic protein (BMP) system in the ovary plays a physiological role as a luteinization inhibitor in growing follicles. BMP-6 secreted from oocytes and granulosa cells can exert an inhibitory effect on follicle-stimulating hormone (FSH) actions by suppressing adenylate cyclase activity downstream of the FSH receptor. The inhibition of FSH-induced progesterone production by BMP-6 is impaired by melatonin treatment in granulosa cells. Intracellular Smad signaling induced by BMP-6 is suppressed by melatonin, suggesting that melatonin has a regulatory role in BMP receptor signaling in granulosa cells...
2018: Vitamins and Hormones
https://www.readbyqxmd.com/read/29421350/long-term-evaluation-of-osteoarthritis-sheep-knee-treated-with-tgf-%C3%AE-3-and-bmp-6-induced-multipotent-stem-cells
#7
C C Ude, B S Shamsul, M H Ng, H C Chen, Htwe Ohnmar, S N Amaramalar, A R Rizal, A Johan, M Y Norhamdan, M Azizi, B S Aminuddin, B H I Ruszymah
BACKGROUND: Hyaline articular cartilage, which protects the bones of diarthrodial joints from forces associated with load bearing, frictions, and impacts has very limited capacities for self-repair. Over the years, the trend of treatments has shifted to regenerations and researchers have been on the quest for a lasting regeneration. We evaluated the treatment of osteoarthritis by chondrogenically induced ADSCs and BMSCs for a long time functional recovery. METHODS: Osteoarthritis was induced at the right knee of sheep by complete resection of ACL and medial meniscus...
April 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29399103/bone-mesenchymal-stem-cells-co-expressing-vegf-and-bmp-6-genes-to-combat-avascular-necrosis-of-the-femoral-head
#8
Hongxing Liao, Zhixiong Zhong, Zhanliang Liu, Liangping Li, Zemin Ling, Xuenong Zou
The aim of the present study was to investigate the potential of bone mesenchymal stem cells (BMSCs) treated with a combination of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-6 (BMP-6) genes for the treatment of avascular necrosis of the femoral head (ANFH). Rat BMSCs were isolated and purified using a density gradient centrifugation method. The purity and characteristics of the BMSCs were detected by cell surface antigens identification using flow cytometry. The experimental groups were administered with one of the following adeno-associated virus (AAV) vector constructs: AAV-green fluorescent protein (AAV-GFP), AAV-BMP-6, AAV-VEGF or AAV-VEGF-BMP-6...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29373940/zein-nanoparticle-as-a-novel-bmp6-derived-peptide-carrier-for-enhanced-osteogenic-differentiation-of-c2c12-cells
#9
Mahvash Hadavi, Sadegh Hasannia, Shahab Faghihi, Farhad Mashayekhi, Homayoun Homazadeh, Seyed Behrooz Mostofi
Zein nanoparticles as a carrier system for BMP6-derived peptide were prepared by liquid-liquid phase separation procedure and characterized with SEM, DLS, FTIR and thermogravimetric methods. After peptide encapsulation, nanoparticle size increased from 236.3 ± 92.2 nm to 379.4 ± 116.8 nm. The encapsulation efficiency of peptide was 72.6% and the release of peptide from Zein nanoparticles was partly sustained in trypsin containing phosphate buffered saline (pH 7.4) for up to 14 days. Peptide-loaded nanoparticles showed similar cell viability compared with blank ones...
January 26, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29323717/chondrogenic-differentiation-of-human-scalp-adipose-derived-stem-cells-in-polycaprolactone-scafold-and-using-freeze-thaw-freeze-method
#10
Rana Moradian Tehrani, Hamed Mirzaie, Javad Verdi, Amirhossein Sahebkar, Mahdi Noureddini, Rasoul Salehi, Behrang Alani, Mojtaba Kianmehr
Human adipose tissue has been identified as a viable alternative source for mesenchymal stem cells. SADSCs were isolated from human scalp biopsy and then were characterized by Flow cytometry. SADSCS expressed CD90, CD44, and CD105 but did not express CD45 surface marker. Growth factors were used for chondrogenesis induction. Histology and immunohistology methods and gene expression by real-time PCR 14 days after induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29311578/promoting-induced-pluripotent-stem-cell-driven-biomineralization-and-periodontal-regeneration-in-rats-with-maxillary-molar-defects-using-injectable-bmp-6-hydrogel
#11
Ke-Hung Chien, Yuh-Lih Chang, Mong-Lien Wang, Jen-Hua Chuang, Ya-Chi Yang, Ming-Cheng Tai, Chien-Ying Wang, Yung-Yang Liu, Hsin-Yang Li, Jiang-Torng Chen, Shou-Yen Kao, Hen-Li Chen, Wen-Liang Lo
Periodontal disease may cause considerable destruction of alveolar bone, periodontal ligaments (PDLs) and cementum and even lead to progressive oral dysfunction. Periodontal tissue regeneration is the ultimate goal of periodontal disease treatment to reconstruct both structures and functions. However, the regenerative efficiency is low, possibly due to the lack of a proper periodontal microenvironment. In this study, we applied an injectable and thermosensitive chitosan/gelatin/glycerol phosphate hydrogel to provide a 3D environment for transplanted stem cells and to enhance stem cell delivery and engraftment...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29129645/incretins-modulate-progesterone-biosynthesis-by-regulating-bone-morphogenetic-protein-activity-in-rat-granulosa-cells
#12
Yuki Nishiyama, Toru Hasegawa, Shiho Fujita, Nahoko Iwata, Satoko Nagao, Takeshi Hosoya, Kenichi Inagaki, Jun Wada, Fumio Otsuka
The effects of incretins on ovarian steroidogenesis have not been clarified. In this study, we investigated the effects of incretins, including GIP and GLP-1, on ovarian steroidogenesis using rat primary granulosa cells. Treatment with incretins significantly suppressed progesterone synthesis in the presence of FSH, and the effect of GIP was more potent than that of GLP-1. In contrast, incretins had no significant effect on estrogen synthesis by rat granulosa cells. In accordance with the effects of incretins on steroidogenesis, GIP and GLP-1 suppressed the expression of progesterogenic factors and enzymes, including StAR, P450scc, 3βHSD, but not P450arom, and cellular cAMP synthesis induced by FSH...
April 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28810575/increased-bone-morphogenetic-protein-6-in-follicular-fluid-and-granulosa-cells-may-correlate-with-fertilization-and-embryo-quality-in-humans
#13
Ying Liang, Qinying Cao, Xing Gao, Huilan Du
Bone morphogenetic protein-6 (BMP-6), which is a member of the transforming growth factor-β superfamily, is associated with the regulation of bone development and various physiological processes. In the present study, the expression of BMP-6 in follicular fluid and granulosa cells (GCs) from pregnant and non-pregnant patients was explored. A total of 44 pregnant patients (pregnant group) and 36 non-pregnant patients (non-pregnant group) were recruited for the present study. The expression of BMP-6 was detected using western blotting and reverse transcription-quantitative polymerase chain reaction...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28684382/a-regulatory-role-of-androgen-in-ovarian-steroidogenesis-by-rat-granulosa-cells
#14
Toru Hasegawa, Yasuhiko Kamada, Takeshi Hosoya, Shiho Fujita, Yuki Nishiyama, Nahoko Iwata, Yuji Hiramatsu, Fumio Otsuka
Excess androgen and insulin-like growth factor (IGF)-I in the ovarian follicle has been suggested to be involved in the pathophysiology of polycystic ovary syndrome (PCOS). Here we investigated the impact of androgen and IGF-I on the regulatory mechanism of ovarian steroidogenesis using rat primary granulosa cells. It was revealed that androgen treatment with dihydrotestosterone (DHT) amplified progesterone synthesis in the presence of FSH and IGF-I, whereas it had no significant effect on estrogen synthesis by rat granulosa cells...
September 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28681971/growth-plate-expression-profiling-large-and-small-breed-dogs-provide-new-insights-in-endochondral-bone-formation
#15
Michelle Teunissen, Frank M Riemers, Dik van Leenen, Marian J A Groot Koerkamp, Björn P Meij, Jacqueline Alblas, Louis C Penning, Alberto Miranda-Bedate, Marianna A Tryfonidou
The difference in the adult height of mammals, and hence in endochondral bone formation, is not yet fully understood and may serve to identify targets for bone and cartilage regeneration. In line with this hypothesis, the intra-species disparity between the adult height of Great Danes and Miniature Poodles was investigated at a transcriptional level. Microarray analysis of the growth plate of five Great Danes and five Miniature Poodles revealed 2,981 unique genes that were differentially expressed, including many genes with an unknown role in skeletal development...
January 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28667462/the-evaluation-of-cartilage-differentiations-using-transforming-growth-factor-beta3-alone-and-with-combination-of-bone-morphogenetic-protein-6-on-adult-stem-cells
#16
C C Ude, H C Chen, M Y Norhamdan, B M Azizi, B S Aminuddin, B H I Ruszymah
In our quest to standardize our formula for a clinical trial, transforming growth factor-beta3 (TGF-β3) alone and in combination with bone morphogenetic protein-6 (BMP-6) were evaluated for their effectiveness in cartilage differentiation. Bone Marrow Stem Cells (BMSCs) and Adipose Derived Stem Cells (ADSCs) were induced to chondrogenic lineage using two different media. Native chondrocytes served as positive control. ADSCs and BMSCs proved multipotency by tri-lineage differentiations. ADSC has significantly higher growth kinetics compare to Chondrocyte only p ≤ 0...
September 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28601648/sequential-igf-1-and-bmp-6-releasing-chitosan-alginate-plga-hybrid-scaffolds-for-periodontal-regeneration
#17
Tuğba Duruel, Anıl Sera Çakmak, Abdullah Akman, Rahime M Nohutcu, Menemşe Gümüşderelioğlu
The goal of periodontal tissue engineering is to repair or regenerate the destructed or lost periodontium by improving functions of cells in the remaining tissue. For continuty of cell growth process, two group of growth factors, i.e. competence factors and progression factors, are needed to act together. However, the short biological half-life of these factors limits their effects on cells and their clinical efficacy. The purpose of this study is to develop different microparticles-loaded chitosan carriers/scaffolds for controlled and sequential delivery of a competence factor, insulin-like growth factor (IGF-1), and progression factor, bone morphogenetic factor-6 (BMP-6)...
November 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28515335/in-situ-bone-tissue-engineering-via-ultrasound-mediated-gene-delivery-to-endogenous-progenitor-cells-in-mini-pigs
#18
Maxim Bez, Dmitriy Sheyn, Wafa Tawackoli, Pablo Avalos, Galina Shapiro, Joseph C Giaconi, Xiaoyu Da, Shiran Ben David, Jayne Gavrity, Hani A Awad, Hyun W Bae, Eric J Ley, Thomas J Kremen, Zulma Gazit, Katherine W Ferrara, Gadi Pelled, Dan Gazit
More than 2 million bone-grafting procedures are performed each year using autografts or allografts. However, both options carry disadvantages, and there remains a clear medical need for the development of new therapies for massive bone loss and fracture nonunions. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would induce efficient bone regeneration and fracture repair. To test this hypothesis, we surgically created a critical-sized bone fracture in the tibiae of Yucatán mini-pigs, a clinically relevant large animal model...
May 17, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28489883/bmp-7-induces-apoptosis-in-human-germinal-center-b-cells-and-is-influenced-by-tgf-%C3%AE-receptor-type-i-alk5
#19
Lise K Bollum, Kanutte Huse, Morten P Oksvold, Baoyan Bai, Vera I Hilden, Lise Forfang, Sun Ok Yoon, Sébastien Wälchli, Erlend B Smeland, June H Myklebust
Selection and maturation of B cells into plasma cells producing high-affinity antibodies occur in germinal centers (GC). GCs form transiently in secondary lymphoid organs upon antigen challenge, and the GC reaction is a highly regulated process. TGF-β is a potent negative regulator, but the influence of other family members including bone morphogenetic proteins (BMPs) is less known. Studies of human peripheral blood B lymphocytes showed that BMP-6 suppressed plasmablast differentiation, whereas BMP-7 induced apoptosis...
2017: PloS One
https://www.readbyqxmd.com/read/28434979/synergistic-effects-of-bmp-2-bmp-6-or-bmp-7-with-human-plasma-fibronectin-onto-hydroxyapatite-coatings-a-comparative-study
#20
COMPARATIVE STUDY
Isabelle Brigaud, Rémy Agniel, Johanne Leroy-Dudal, Sabrina Kellouche, Arnaud Ponche, Tahar Bouceba, Natalia Mihailescu, Mihai Sopronyi, Eric Viguier, Carmen Ristoscu, Felix Sima, Ion N Mihailescu, Ana Claudia O Carreira, Mari Cleide Sogayar, Olivier Gallet, Karine Anselme
Design of new osteoinductive biomaterials to reproduce an optimized physiological environment capable of recruiting stem cells and instructing their fate towards the osteoblastic lineage has become a priority in orthopaedic surgery. This work aims at evaluating the bioactivity of BMP combined with human plasma fibronectin (FN/BMP) delivered in solution or coated onto titanium-hydroxyapatite (TiHA) surfaces. Herein, we focus on the comparison of in vitro osteogenic efficacy in mouse C2C12 pre-osteoblasts of three BMP members, namely: BMP-2, BMP-6 and BMP-7...
June 2017: Acta Biomaterialia
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