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https://www.readbyqxmd.com/read/27242439/low-dose-endothelial-monocyte-activating-polypeptide-ii-induced-autophagy-by-down-regulating-mir-20a-in-u-87-and-u-251-glioma-cells
#1
Jiajia Chen, Libo Liu, Yunhui Liu, Xiaobai Liu, Chengbin Qu, Fanjie Meng, Jun Ma, Yang Lin, Yixue Xue
Preliminary studies have shown that endothelial-monocyte-activating polypeptide-II (EMAP-II) induces autophagy and inhibits the viability of glioma cells via an unknown molecular mechanism. This study explored the possible mechanisms associated with EMAP-II-induced autophagy in glioma cells by regulation of the expression of microRNA-20a (miR-20a). EMAP-II effectively inhibited the viability, migration and invasion of human U-87 and U-251 glioma cells. EMAP-II also up-regulated the expression level of autophagy biomarker microtubule-associated protein one light chain 3 (LC3)-II/I, autophagy related gene ATG7 and ATG5, but down-regulated autophagy substrate P62/SQSTM1 protein expression...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27089716/-serum-level-of-endothelial-monocyte-activating-polypeptide-ii-in-childhood-onset-type-1-diabetic-patients-and-obese-adolescents
#2
L A Mogylnytska
The atherosclerotic process begins in adolescence, and its progression is determined by the same risk factors as in adults. Endothelial monocyte activating polypeptide-II (EMAP-II) is a multifunctional cytokine with proinflammatory and antiangiogenetic activity that may play a pathogenic role in the development of endothelial dysfunction and atherosclerosis. The aim of our study was to determine the serum level of EMAP-II in childhood-onset type 1 diabetic patients and obese adolescents. We found increased of serum level of EMAP-II in childhood-onset type 1 diabetic patients and in patients with obesity that do not suffer from diabetes...
July 2015: Likars'ka Sprava
https://www.readbyqxmd.com/read/26648842/autophagy-induction-by-endothelial-monocyte-activating-polypeptide-ii-contributes-to-the-inhibition-of-malignant-biological-behaviors-by-the-combination-of-emap-ii-with-rapamycin-in-human-glioblastoma
#3
Jun Ma, Fanjie Meng, Shuai Li, Libo Liu, Lini Zhao, Yunhui Liu, Yi Hu, Zhen Li, Yilong Yao, Zhuo Xi, Hao Teng, Yixue Xue
This study aims to investigate the effect of endothelial-monocyte activating polypeptide II (EMAP II) on human glioblastoma (GBM) cells and glioblastoma stem cells (GSCs) as well as its possible mechanisms. In this study, EMAP II inhibited the cell viability and decreased the mitochondrial membrane potential in human GBM cells and GSCs, and autophagy inhibitor 3-methyl adenine (3-MA) blocked these effects. Autophagic vacuoles were formed in these cells after EMAP II treatment and this phenomenon was blocked by 3-MA...
2015: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/26592408/protein-protein-docking-using-emap-in-charmm-and-support-vector-machine-application-to-ab-ag-complexes
#4
Jon D Wright, Karen Sargsyan, Xiongwu Wu, Bernard R Brooks, Carmay Lim
In this work, we have (i) evaluated the ability of the EMAP method implemented in the CHARMM program to generate the correct conformation of Ab/Ag complex structures and (ii) developed a support vector machine (SVM) classifier to detect native conformations among the thousands of refined Ab/Ag configurations using the individual components of the binding free energy based on a thermodynamic cycle as input features in training the SVM. Tests on 24 Ab/Ag complexes from the protein-protein docking benchmark version 3...
September 10, 2013: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/26521255/low-dose-endothelial-monocyte-activating-polypeptide-ii-increases-blood-tumor-barrier-permeability-by-activating-the-rhoa-rock-pi3k-signaling-pathway
#5
Zhen Li, Xiao-Bai Liu, Yun-Hui Liu, Yi-Xue Xue, Jing Liu, Hao Teng, Zhuo Xi, Yi-Long Yao
Previous studies have demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) can increase blood-tumor barrier (BTB) permeability via both paracellular and transcellular pathways. In addition, we revealed that the RhoA/Rho kinase (ROCK) signaling pathway is involved in EMAP-II-induced BTB opening. This study further investigated the exact mechanisms by which the RhoA/ROCK signaling pathway affects EMAP-II-induced BTB hyperpermeability. In an in vitro BTB model, low-dose EMAP-II significantly activated phosphatidylinositol-3-kinase (PI3K) in rat brain microvascular endothelial cells (RBMECs) at 0...
June 2016: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/26495731/-endothelial-monocyteactivating-factor-ii-cancels-oxidative-stress-constitutive-nos-uncoupling-and-induced-violations-of-cardiac-hemodynamics-in-hypertension-part-ii
#6
N A Dorofeyeva, A V Kotsuruba, L A Mogilnitskaya, A E Malyna, A I Kornelyuk, V F Sagach
The purpose of this study was to investigate the effect of EMAP II on free radical state of the heart and blood vessels, to restore cNOS coupling and cardiac hemodynamics in spontaneously hypertensive rats. It was found that, due to the combined inhibition of oxidative and nitrosative stress, EMAP I quickly restores impaired in hypertension constitutive de novo synthesis of NO by restoring cNOS coupling. Restoration by EMAP II of constitutive de novo synthesis NO abolished cardiac and endothelial dysfunction in spontaneously hypertensive rats...
2015: Fiziolohichnyĭ Zhurnal
https://www.readbyqxmd.com/read/26358039/low-dose-endothelial-monocyte-activating-polypeptide-ii-induces-blood-tumor-barrier-opening-via-the-camp-pka-rac1-pathway
#7
Zhen Li, Xiao-bai Liu, Yun-hui Liu, Yi-xue Xue, Jing Liu, Hao Teng, Zhuo Xi, Yi-long Yao
Previous studies have demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) induces blood-tumor barrier (BTB) hyperpermeability via both paracellular and transcellular pathways. In a recent study, we revealed that cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)-dependent signaling pathway is involved in EMAP-II-induced BTB hyperpermeability. This study further investigated the exact mechanisms through which the cAMP/PKA-dependent signaling pathway affects EMAP-II-induced BTB hyperpermeability...
February 2016: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/26142824/endothelial-monocyte-activating-polypeptide-ii-in-children-and-adolescents-with-type-1-diabetes-mellitus-relation-to-micro-vascular-complications
#8
Amira A M Adly, Eman A Ismail, Lamis M Tawfik, Fatma S E Ebeid, Asmaa A S Hassan
OBJECTIVES: Endothelial monocyte-activating polypeptide II (EMAP II) is a multifunctional polypeptide with proinflammatory and antiangiogenic activity. Hyperglycemia and dyslipidemia appears to be significant factors contributing to increased EMAP-II levels. We determined serum EMAP II in children and adolescents with type 1 diabetes as a potential marker for micro-vascular complications and assessed its relation to inflammation and glycemic control. METHODS: Eighty children and adolescents with type 1 diabetes were divided into 2 groups according to the presence of micro-vascular complications and compared with 40 healthy controls...
December 2015: Cytokine
https://www.readbyqxmd.com/read/26087743/roles-of-serine-threonine-phosphatases-in-low-dose-endothelial-monocyte-activating-polypeptide-ii-induced-opening-of-blood-tumor-barrier
#9
Zhen Li, Xiao-Bai Liu, Yun-hui Liu, Yi-xue Xue, Ping Wang, Li-bo Liu, Jing Liu, Yi-long Yao, Jun Ma
Previous studies have demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) induces blood-tumor barrier (BTB) opening via RhoA/Rho kinase/PKC-α/β signaling pathway. In a recent study, we revealed that low-dose EMAP-II induced significant increases in expression levels of serine/threonine (Ser/Thr) phosphatase (PP)1 and 2A in rat brain microvascular endothelial cells (RBMECs) of BTB model. In addition, PKC-ζ/PP2A signaling pathway is involved in EMAP-II-induced BTB hyperpermeability...
September 2015: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/26044663/functions-for-the-camp-epac-rap1-signaling-pathway-in-low-dose-endothelial-monocyte-activating-polypeptide-ii-induced-opening-of-blood-tumor-barrier
#10
Zhen Li, Xiao-Bai Liu, Yun-Hui Liu, Yi-Xue Xue, Ping Wang, Li-Bo Liu, Yi-Long Yao, Jun Ma
Previous studies have demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) induces blood-tumor barrier (BTB) hyperpermeability via both paracellular and transcellular pathways. In a recent study, we revealed that cAMP/PKA-dependent and cAMP/PKA-independent signaling pathways are both involved in EMAP-II-induced BTB hyperpermeability. The present study further investigated the exact mechanisms through which the cAMP/PKA-independent signaling pathway affects EMAP-II-induced BTB hyperpermeability...
September 2015: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/26040042/-endothelial-monocyte-activating-polypeptide-ii-properties-functions-and-pathogenetic-significance
#11
L A Mogylnytska
Endothelial dysfunction is implicated in the pathogenesis of diabetes and atherosclerosis. Endothelial monocyte-activating polypeptide-II (EMAP-II) is a multifunctional polypeptide with proinflammatory and antiangiogenic activity. EMAP-II induces procoagulant activity on the surface of endothelial cells, increases expression of E- and P-selectins and tumor necrosis factor-1, directs migration of monocytes and neutrophils, induces apoptosis in endothelial cells. The mechanisms of effects on endothelial cells, inflammatory action, anti-tumor properties, pathogenic role in diseases of the central nervous system involved in the development of the lungs during embryogenesis and pathogenic role in diseases of the lungs, in the development of cardiovascular disease...
2015: Fiziolohichnyĭ Zhurnal
https://www.readbyqxmd.com/read/25724651/the-n-terminus-of-pro-endothelial-monocyte-activating-polypeptide-ii-emap-ii-regulates-its-binding-with-the-c-terminus-arginyl-trna-synthetase-and-neurofilament-light-protein
#12
Haiming Xu, Nikolay L Malinin, Niranjan Awasthi, Roderich E Schwarz, Margaret A Schwarz
Pro-endothelial monocyte-activating polypeptide II (EMAP II), one component of the multi-aminoacyl tRNA synthetase complex, plays multiple roles in physiological and pathological processes of protein translation, signal transduction, immunity, lung development, and tumor growth. Recent studies have determined that pro-EMAP II has an essential role in maintaining axon integrity in central and peripheral neural systems where deletion of the C terminus of pro-EMAP II has been reported in a consanguineous Israeli Bedouin kindred suffering from Pelizaeus-Merzbacher-like disease...
April 10, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25600803/endothelial-monocyte-activating-polypeptide-ii-induces-rat-c6-glioma-cell-apoptosis-via-the-mitochondrial-pathway
#13
Li-Bo Liu, Hui Xie, Yi-Xue Xue, Yun-Hui Liu, Zhen Li, Ping Wang
The present study was performed to examine whether Endothelial-monocyte-activating polypeptide II (EMAP II) could inhibit glioma growth by inducing rat brain glioma C6 cells apoptosis. The results revealed that the EMAP II decreased cell viability of rat C6 glioma cells in a time-dependent manner. Apoptotic proportion was increased gradually after EMAP II. EMAP II induced the decrease in mitochondrial membrane potential and the release of cytochrome c into the cytosol, followed by activation of caspase-9 and caspase-3...
February 20, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25592443/low-dose-endothelial-monocyte-activating-polypeptide-ii-increases-permeability-of-blood-tumor-barrier-via-a-pkc-%C3%AE-pp2a-dependent-signaling-mechanism
#14
Zhen Li, Yun-Hui Liu, Xiao-Bai Liu, Yi-Xue Xue, Ping Wang, Li-Bo Liu
Our previous study demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) induces blood-tumor barrier (BTB) opening via the RhoA/Rho kinase/protein kinase C (PKC)-α/β signaling pathway and that PKC-ζ is involved in this process via other mechanisms. In the present study, using an in vitro BTB model, we detected the exact signaling mechanisms by which PKC-ζ activation affects EMAP-II-induced BTB hyperpermeability. Our results showed that three types of serine/threonine (Ser/Thr) protein phosphatases (PPs), namely PP1, PP2A, and PP2B, were expressed by rat brain microvascular endothelial cells (RBMECs)...
February 15, 2015: Experimental Cell Research
https://www.readbyqxmd.com/read/25456857/blockade-of-emap-ii-protects-cardiac-function-after-chronic-myocardial-infarction-by-inducing-angiogenesis
#15
Chujun Yuan, Lin Yan, Pallavi Solanki, Stephen F Vatner, Dorothy E Vatner, Margaret A Schwarz
Promoting angiogenesis is a key therapeutic target for protection from chronic ischemic cardiac injury. Endothelial-Monocyte-Activating-Polypeptide-II (EMAP II) protein, a tumor-derived cytokine having anti-angiogenic properties in cancer, is markedly elevated following myocardial ischemia. We examined whether neutralization of EMAP II induces angiogenesis and has beneficial effects on myocardial function and structure after chronic myocardial infarction (MI). EMAP II antibody (EMAP II AB), vehicle, or non-specific IgG (IgG) was injected ip at 30 min and 3, 6, and 9 days after permanent coronary artery occlusion in mice...
February 2015: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/25416651/role-of-camp-dependent-protein-kinase-a-activity-in-low-dose-endothelial-monocyte-activating-polypeptide-ii-induced-opening-of-blood-tumor-barrier
#16
Zhen Li, Xiao-bai Liu, Yun-hui Liu, Yi-xue Xue, Ping Wang, Li-bo Liu
Our previous studies demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) can selectively increase the permeability of blood-tumor barrier (BTB). In addition, low-dose EMAP-II significantly decreases the cyclic adenosine monophosphate (cAMP) concentration and the protein kinase A (PKA) expression level in tumor tissues in the rat C6 glioma model. In this study, an in vitro BTB model was used to investigate the potential role of cAMP/PKA signaling cascade in EMAP-II-induced BTB hyperpermeability...
May 2015: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/24809179/-serum-levels-of-endothelial-monocyte-activating-polypeptide-ii-in-type-2-diabetes-mellitus
#17
L A Mohyl'nyts'ka
Endothelial dysfunction is implicated in the pathogenesis of macrovascular complications of diabetes and atherosclerosis. Endothelial monocyte-activating polypeptide-II (EMAP-II) is a multifunctional polypeptide with proinflammatory and antiangiogenic activity. The aim of this study was to investigate the serum level of EMAP-II in obese and nonobese patients with type 2 diabetes. We found an increase of serum level of EMAP-II in obese diabetic patients compared to obese subjects without diabetes. Similar alterations were observed also in non-obese diabetic patients compared to control subjects...
2014: Fiziolohichnyĭ Zhurnal
https://www.readbyqxmd.com/read/24792437/anti-neoplastic-activity-of-low-dose-endothelial-monocyte-activating-polypeptide-ii-results-from-defective-autophagy-and-g2-m-arrest-mediated-by-pi3k-akt-foxo1-axis-in-human-glioblastoma-stem-cells
#18
Jing Liu, Libo Liu, Yixue Xue, Fanjie Meng, Shuai Li, Ping Wang, Yunhui Liu
Glioblastoma multiforme (GBM) is a life-threatening brain tumor with fatal recurrence, for which glioblastoma stem cells (GSCs) are held responsible. Though endothelial-monocyte activating polypeptide-II (EMAP-II) has been confirmed as a possible antitumor agent that can induce apoptosis of endothelial cells and inhibit tumor angiogenesis, the direct cytotoxicity by EMAP-II on tumor cells and its underlying mechanism are largely unknown. In the present study, it was demonstrated that low-dose (0.05 nM) EMAP-II reduces cell viability and mitochondrial membrane potential in vitro...
June 15, 2014: Biochemical Pharmacology
https://www.readbyqxmd.com/read/24619875/vascular-mediators-in-chronic-lung-disease-of-infancy-role-of-endothelial-monocyte-activating-polypeptide-ii-emap-ii
#19
REVIEW
Charitharth Vivek Lal, Margaret A Schwarz
Bronchopulmonary dysplasia (BPD) is a chronic lung disease of prematurity. Over the years, the BPD phenotype has evolved, but despite various advances in neonatal management approaches, the reduction in the BPD burden is minimal. With the advent of surfactant, glucocorticoids, and new ventilation strategies, BPD has evolved from a disease of structural injury into a new BPD, marked by an arrest in alveolar growth in the lungs of extremely premature infants. This deficient alveolar growth has been associated with a diminution of pulmonary vasculature...
March 2014: Birth Defects Research. Part A, Clinical and Molecular Teratology
https://www.readbyqxmd.com/read/24526454/low-dose-endothelial-monocyte-activating-polypeptide-ii-increases-permeability-of-blood-tumor-barrier-by-caveolae-mediated-transcellular-pathway
#20
Zhen Li, Yun-hui Liu, Yi-xue Xue, Li-bo Liu, Ping Wang
Low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) can selectively increase blood-tumor barrier (BTB) permeability via the paracellular pathway. The role of the transcellular pathway in this process is unclear. This study was conducted to evaluate the potential involvement of the transcellular pathway in EMAP-II-induced opening of the BTB and to identify the associated mechanisms. Evans blue extravasation test was used to measure changes in BTB permeability after EMAP-II (80 ng/kg) administration in a rat model of C6 glioma...
March 2014: Journal of Molecular Neuroscience: MN
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