Erica N Montano, Moumita Bose, Lihong Huo, Gantsetseg Tumurkhuu, Gabriela De Los Santos, Brianna Simental, Aleksandr B Stotland, Janet Wei, C Noel Bairey Merz, Jo Suda, Gislaine Martins, Sarfaraz Lalani, Kate Lawrenson, Yizhou Wang, Sarah Parker, Swamy Venuturupalli, Mariko Ishimori, Daniel J Wallace, Caroline A Jefferies
OBJECTIVE: Investigate the hypothesis that interferon (IFN) stimulated gene (ISG) expression in systemic lupus erythematosus (SLE) monocytes is linked to changes in metabolic reprogramming and epigenetic regulation of ISG expression. METHODS: Monocytes from healthy volunteers and SLE patients at baseline or following IFNα treatment were analyzed by extracellular flux analysis, proteomics, metabolomics, chromatin immunoprecipitation and gene expression. The histone demethylases KDM6A/B were inhibited using GSK-J4...
October 6, 2023: Arthritis & Rheumatology