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https://www.readbyqxmd.com/read/28239975/preparing-to-read-the-ubiquitin-code-top-down-analysis-of-unanchored-ubiquitin-tetramers
#1
Amanda E Lee, Lucia Geis-Asteggiante, Emma K Dixon, Meredith Miller, Yan Wang, David Fushman, Catherine Fenselau
The characterization of polyubiquitin chains has been an analytical challenge for several decades. It has been shown that anchored and unanchored polyubiquitin chains with different isopeptide linkages and lengths exhibit a wide range of profoundly different cellular functions. However, structure function studies have been hindered by the difficulty of characterizing these complex chain structures. This report presents a broadly applicable workflow to characterize ubiquitin tetramers without the need for genetic mutations or reiterative immunoprecipitations...
August 2016: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28239881/how-chronic-disease-affects-children-s-views-on-being-ill-and-healthy-a-comparative-study
#2
Lígia Lima, Vanessa Silva, Marina S Lemos
BACKGROUND: Understanding children's views of illness and health is vital to a child-centred approach in patient education and care. Paediatric healthcare providers should incorporate children's views of health and illness to enable children and their families to participate in self-management plans and achieve better health outcomes. However, there is limited scientific knowledge regarding the views of children with specific diseases. METHOD: This comparative study investigated whether and in what ways the experience of having different chronic diseases influenced children's views of being ill and healthy...
February 26, 2017: Scandinavian Journal of Caring Sciences
https://www.readbyqxmd.com/read/28239880/long-term-use-of-oral-nucleos-t-ide-analogues-for-chronic-hepatitis-b-does-not-increase-cancer-risk-a-cohort-study-of-44-494-subjects
#3
G L-H Wong, Y-K Tse, T C-F Yip, H L-Y Chan, K K-F Tsoi, V W-S Wong
BACKGROUND: Patients with chronic hepatitis B (CHB) need long-term antiviral treatment with nucleos(t)ide analogues (NA). Animal studies suggest that some NA may increase cancer risk, but human data are lacking. AIM: To investigate cancer risks in patients with or without NA treatment. METHODS: We conducted a territory-wide cohort study using the database from Hospital Authority in Hong Kong. The diagnosis of CHB and various malignancies was based on the International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) diagnosis codes between 2000 and 2012...
February 27, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28239824/long-non-coding-rna-cche1-overexpression-predicts-a-poor-prognosis-for-cervical-cancer
#4
Y Chen, C-X Wang, X-X Sun, C Wang, T-F Liu, D-J Wang
OBJECTIVE: Our study is intended to explore the correlation of long non-coding RNA CCHE1 (CCHE1) and clinicopathological factors of cervical cancer patients, and evaluate the impact of CCHE1 on the prognosis of cervical cancer. PATIENTS AND METHODS: The CCHE1 expression in cervical cancer tissues was examined by quantitative Real-time PCR (qRT-PCR) and its correlation with clinicopathological features was also analyzed. A Kaplan-Meier survival curve was generated following a log-rank test...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239821/analysis-of-circulating-long-non-coding-rna-uca1-as-potential-biomarkers-for-diagnosis-and-prognosis-of-osteosarcoma
#5
J-J Wen, Y-D Ma, G-S Yang, G-M Wang
OBJECTIVE: The aim of this investigation was to examine the potential usefulness of long non-coding RNA UCA1 (UCA1) as a biomarker for diagnosis and prognosis in osteosarcoma. PATIENTS AND METHODS: The expression level of UCA1 was determined using TaqMan real-time PCR in human osteosarcoma tissues and patients' sera. Next, we investigated to clarify the relationship of UCA1 with clinicopathological features. The receiver operating characteristic (ROC) curve was performed to estimate the diagnostic value of UCA1...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239820/long-non-coding-rna-neat1-acts-as-oncogene-in-nsclc-by-regulating-the-wnt-signaling-pathway
#6
S-J Sun, Q Lin, J-X Ma, W-W Shi, B Yang, F Li
OBJECTIVE: The present study aimed to explore the role of long non-coding RNA NEAT1 (NEAT1) in mediating non-small cell lung cancer (NSCLC) cell migration and invasion, as well as the underlying regulatory mechanisms. PATIENTS AND METHODS: The NEAT1 expression in NSCLC tissues and cell lines was measured using reverse transcription‑quantitative polymerase chain reaction (RT-qPCR). The relationships between NEAT1 expression and clinicopathological parameters were examined by chi-square test...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239816/long-non-coding-rna-carlo-5-upregulation-associates-with-poor-prognosis-in-patients-suffering-gastric-cancer
#7
J-N Liu, Y-M Shangguan
OBJECTIVE: Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development. The present study aims to explore the clinical significance of long noncoding RNA CARLo-5 (CARLo-5) in gastric cancer. PATIENTS AND METHODS: CARLo-5 expression in normal gastric tissue, gastric cancer were quantified by Quantitative reverse transcription PCR. The relationship between CARLo-5 expression and the clinical features of gastric cancer was assessed...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239676/intact-ventral-striatal-prediction-error-signaling-in-medicated-schizophrenia-patients
#8
Adam J Culbreth, Andrew Westbrook, Ziye Xu, Deanna M Barch, James A Waltz
BACKGROUND: Midbrain dopaminergic neurons code a computational quantity, reward prediction error (RPE), which has been causally related to learning. Recently, this insight has been leveraged to link phenomenological and biological levels of understanding in psychiatric disorders, such as schizophrenia. However, results have been mixed, possibly due to small sample sizes. Here we present results from two studies with relatively large Ns to assess VS RPE in schizophrenia. METHODS: In the current study we analyzed data from two independent studies, involving a total of 87 chronic medicated schizophrenia patients and 61 controls...
September 2016: Biological Psychiatry: Cognitive Neuroscience and Neuroimaging
https://www.readbyqxmd.com/read/28239625/discrepancies-in-communication-versus-documentation-of-weight-management-benchmarks-analysis-of-recorded-visits-with-latino-children-and-associated-health-record-documentation
#9
Christy B Turer, Sarah E Barlow, Sergio Montaño, Glenn Flores
To examine gaps in communication versus documentation of weight-management clinical practices, communication was recorded during primary care visits with 6- to 12-year-old overweight/obese Latino children. Communication/documentation content was coded by 3 reviewers using communication transcripts and health-record documentation. Discrepancies in communication/documentation content codes were resolved through consensus. Bivariate/multivariable analyses examined factors associated with discrepancies in benchmark communication/documentation...
2017: Global Pediatric Health
https://www.readbyqxmd.com/read/28239554/the-development-and-characterization-of-a-novel-yet-simple-3d-printed-tool-to-facilitate-phantom-imaging-of-photoacoustic-contrast-agents
#10
Santiago J Arconada-Alvarez, Jeanne E Lemaster, Junxin Wang, Jesse V Jokerst
We report a new approach to preparing phantoms using 3D printing. This device supports plastic tubing containing the contrast agent and is immersed in a solution with absorption or scattering properties that mimic tissue. Up to 12 tubing samples could be placed in the device with sample-to-sample spacing as low as 0.3 mm and at a constant distance from the transducer (±0.16 mm), which is critical in validating photoacoustic contrast agents. We also studied different types of tubing and found that tubing with a larger outside diameter has more inherent signal...
March 2017: Photoacoustics
https://www.readbyqxmd.com/read/28239514/a-fragmented-adeno-associated-viral-dual-vector-strategy-for-treatment-of-diseases-caused-by-mutations-in-large-genes-leads-to-expression-of-hybrid-transcripts
#11
Michelle E McClements, Peter Charbel Issa, Véronique Blouin, Robert E MacLaren
OBJECTIVE: Dual vector AAV systems are being utilised to enable gene therapy for disorders in which the disease gene is too large to fit into a single capsid. Fragmented adeno-associated viral (fAAV) vectors containing single inverted terminal repeat truncated transgenes have been considered as one such gene replacement strategy. Here we aim to add to the current understanding of the molecular mechanisms employed by fAAV dual vector systems. METHODS: Oversized (>8kb) transgene constructs containing ABCA4 coding sequence were packaged as truncated fragments <5kb in size into various AAV serotypes...
November 14, 2016: Journal of Genetic Syndrome & Gene Therapy
https://www.readbyqxmd.com/read/28239512/exploring-the-continuum-of-vaccine-hesitancy-between-african-american-and-white-adults-results-of-a-qualitative-study
#12
Sandra Quinn, Amelia Jamison, Donald Musa, Karen Hilyard, Vicki Freimuth
Vaccine delay and refusal present very real threats to public health. Since even a slight reduction in vaccination rates could produce major consequences as herd immunity is eroded, it is imperative to understand the factors that contribute to decision-making about vaccines. Recent scholarship on the concept of "vaccine hesitancy" emphasizes that vaccine behaviors and beliefs tend to fall along a continuum from refusal to acceptance. Most research on hesitancy has focused on parental decision-making about childhood vaccines, but could be extended to explore decision-making related to adult immunization against seasonal influenza...
December 29, 2016: PLoS Currents
https://www.readbyqxmd.com/read/28239485/protocol-for-the-evaluation-of-a-digital-storytelling-approach-to-address-stigma-and-improve-readiness-to-seek-services-among-veterans
#13
Brian E Bunnell, Tatiana M Davidson, Jessica L Hamblen, Danna L Cook, Anouk L Grubaugh, Brian E Lozano, Peter W Tuerk, Kenneth J Ruggiero
BACKGROUND: Research suggests that at least 10% of veterans returning from Iraq and Afghanistan meet criteria for posttraumatic stress disorder (PTSD) related to their military experiences. National dissemination initiatives have increased veterans' access to best-practice interventions. However, treatment-seeking remains low among veterans with PTSD, often due to perceived stigma and other associated barriers. The National Center for PTSD recently developed and launched AboutFace, a digital storytelling (DST) resource designed to help veterans recognize PTSD and motivate them to seek evidence-based treatment...
2017: Pilot and Feasibility Studies
https://www.readbyqxmd.com/read/28239478/genomic-analysis-of-a-streptococcus-pyogenes-strain-causing-endocarditis-in-a-child
#14
M Beye, K El Karkouri, N Labas, D Raoult, P-E Fournier
We sequenced the genome of Streptococcus pyogenes strain G773 that caused an infective endocarditis in a 4-year-old boy suffering from acute endocarditis. The 1.9-Mb genome exhibited a specific combination of virulence factors including a complete integrative and conjugative element, sp2905, previously described as incomplete in S. pyogenes, and five bacteriocin-coding genes. However, strain G773 lacked a CRISPR-Cas system.
May 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28239422/differential-regulation-of-riboflavin-supply-genes-in-vibrio-cholerae
#15
Ignacio Sepúlveda Cisternas, Alexia Torres, Andrés Fuentes Flores, Víctor Antonio García Angulo
BACKGROUND: Riboflavin is the precursor of important redox cofactors such as flavin mononucleotide (FMN) and flavin adenine dinucleotide, required for several biological processes. Vibrio cholerae, a pathogenic bacterium responsible for the cholera disease, possesses the ability to biosynthesize de novo as well as to uptake riboflavin through the riboflavin biosynthetic pathway (RBP) and the RibN importer, respectively. The intra-organism relationship between riboflavin biosynthesis and uptake functions has not been studied...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28239372/identification-by-genome-mining-of-a-type-i-polyketide-gene-cluster-from-streptomyces-argillaceus-involved-in-the-biosynthesis-of-pyridine-and-piperidine-alkaloids-argimycins-p
#16
Suhui Ye, Brian Molloy, Alfredo F Braña, Daniel Zabala, Carlos Olano, Jesús Cortés, Francisco Morís, José A Salas, Carmen Méndez
Genome mining of the mithramycin producer Streptomyces argillaceus ATCC 12956 revealed 31 gene clusters for the biosynthesis of secondary metabolites, and allowed to predict the encoded products for 11 of these clusters. Cluster 18 (renamed cluster arp) corresponded to a type I polyketide gene cluster related to the previously described coelimycin P1 and streptazone gene clusters. The arp cluster consists of fourteen genes, including genes coding for putative regulatory proteins (a SARP-like transcriptional activator and a TetR-like transcriptional repressor), genes coding for structural proteins (three PKSs, one aminotransferase, two dehydrogenases, two cyclases, one imine reductase, a type II thioesterase, and a flavin reductase), and one gene coding for a hypothetical protein...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28239348/genetic-determinants-of-parkinson-s-disease-can-they-help-to-stratify-the-patients-based-on-the-underlying-molecular-defect
#17
REVIEW
Sara Redenšek, Maja Trošt, Vita Dolžan
Parkinson's disease (PD) is a sporadic progressive neurodegenerative brain disorder with a relatively strong genetic background. We have reviewed the current literature about the genetic factors that could be indicative of pathophysiological pathways of PD and their applications in everyday clinical practice. Information on novel risk genes is coming from several genome-wide association studies (GWASs) and their meta-analyses. GWASs that have been performed so far enabled the identification of 24 loci as PD risk factors...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28239300/mir-7-inhibits-progression-of-hepatocarcinoma-by-targeting-klf-4-and-promises-a-novel-diagnostic-biomarker
#18
Weizhong Wu, Sanguang Liu, Yunfei Liang, Zegao Zhou, Xueqing Liu
BACKGROUND: MicroRNAs are 22-24 nt non-coding RNAs that bind to the 3' UTR of target mRNAs, thereby inducing mRNA degradation or inhibiting mRNA translation. Due to their implication in the regulation of post-transcriptional processes, the role of miRNAs in hepatocellular carcinoma (HCC) has been extensively studied. However, the function of miR-7 in HCC remains to be demonstrated. METHODS: 50 paired HCC tissues and matched peritumor tissues from patients were collected...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28239231/genome-wide-discovery-of-dna-polymorphisms-in-mei-prunus-mume-sieb-et-zucc-an-ornamental-woody-plant-with-contrasting-tree-architecture-and-their-functional-relevance-for-weeping-trait
#19
Jie Zhang, Kai Zhao, Dan Hou, Junhuo Cai, Qixiang Zhang, Tangren Cheng, Huitang Pan, Weiru Yang
Next-generation sequencing technologies provide opportunities to ascertain the genetic basis of phenotypic differences, even in the closely related cultivars via detection of large amount of DNA polymorphisms. In this study, we performed whole-genome re-sequencing of two mei cultivars with contrasting tree architecture. 75.87 million 100 bp pair-end reads were generated, with 92 % coverage of the genome. Re-sequencing data of two former upright mei cultivars were applied for detecting DNA polymorphisms, since we were more interested in variations conferring weeping trait...
2017: Plant Molecular Biology Reporter
https://www.readbyqxmd.com/read/28239230/experimental-analysis-of-steel-beams-subjected-to-fire-enhanced-by-brillouin-scattering-based-fiber-optic-sensor-data
#20
Yi Bao, Yizheng Chen, Matthew S Hoehler, Christopher M Smith, Matthew Bundy, Genda Chen
This paper presents high temperature measurements using a Brillouin scattering-based fiber optic sensor and the application of the measured temperatures and building code recommended material parameters into enhanced thermomechanical analysis of simply supported steel beams subjected to combined thermal and mechanical loading. The distributed temperature sensor captures detailed, nonuniform temperature distributions that are compared locally with thermocouple measurements with less than 4.7% average difference at 95% confidence level...
January 2017: Journal of Structural Engineering (New York, N.Y.)
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