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https://www.readbyqxmd.com/read/28103795/lineage-specific-duplication-of-amphioxus-retinoic-acid-degrading-enzymes-cyp26-resulted-in-sub-functionalization-of-patterning-and-homeostatic-roles
#1
João E Carvalho, Maria Theodosiou, Jie Chen, Pascale Chevret, Susana Alvarez, Angel R De Lera, Vincent Laudet, Jenifer C Croce, Michael Schubert
BACKGROUND: During embryogenesis, tight regulation of retinoic acid (RA) availability is fundamental for normal development. In parallel to RA synthesis, a negative feedback loop controlled by RA catabolizing enzymes of the cytochrome P450 subfamily 26 (CYP26) is crucial. In vertebrates, the functions of the three CYP26 enzymes (CYP26A1, CYP26B1, and CYP26C1) have been well characterized. By contrast, outside vertebrates, little is known about CYP26 complements and their biological roles...
January 19, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28069432/microrna-expression-changes-after-lipopolysaccharide-treatment-in-gills-of-amphioxus-branchiostoma-belcheri
#2
Xin Liao, Liu Yang, Qilin Zhang, Junyuan Chen
Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS). We determined the time point for the peak of immune response in amphioxus after LPS challenge by evaluating the expression of Branchiostoma belcheri toll-like receptor 1, NF-κb (c-Rel), and big defensin which react with pathogen-associated molecular patterns(PAMPs)...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28069430/functional-characterization-of-avidins-in-amphioxus-branchiostoma-japonicum-evidence-for-a-dual-role-in-biotin-binding-and-immune-response
#3
Xiaomin Guo, Jiajing Xin, Peng Wang, Xiaoyuan Du, Guangdong Ji, Zhan Gao, Shicui Zhang
Avidin is well known for its high affinity to biotin and has been found in many egg-laying vertebrate species. However, little is known about avidin in invertebrate species to date. Here we clearly showed the presence of two avidin genes, Bjavidin1 and Bjavidin2, in the amphioxus Branchiostoma japonicum, the first ones in non-vertebrate animals. We also showed that the expression of both Bjavidin1 and Bjavidin2 were inducible by progesterone, LTA and LPS. Moreover, we demonstrated for the first time that in addition to biotin-binding, the recombinant proteins rBjAVIDIN1 and rBjAVIDIN2 were not only able to interact with Gram-positive and negative bacteria as well as their conserved surface components LTA and LPS but also to enhance phagocytosis of bacteria by macrophages, suggesting that BjAVIDIN1 and BjAVIDIN2 both function as pattern recognition receptors and opsonins...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28062302/calcitonin-typical-suppression-of-osteoclastic-activity-by-amphioxus-calcitonin-superfamily-peptides-and-insights-into-the-evolutionary-conservation-and-diversity-of-their-structures
#4
Toshio Sekiguchi, Akira Shiraishi, Honoo Satake, Kenji Kuwasako, Hiroki Takahashi, Masayuki Sato, Makoto Urata, Shuichi Wada, Masato Endo, Takahiro Ikari, Atsuhiko Hattori, Ajai K Srivastav, Nobuo Suzuki
Calcitonin (CT) is a hormone that decreases serum calcium level by suppressing osteoclastic activity in the vertebrate bone. In vertebrates, the structure-function relationship of CTs has been studied extensively. We recently identified three CT superfamily peptides, Bf-CTFP1 to 3, and clarified the molecular and functional characteristics of their receptor and receptor activity-modifying protein in amphioxus, Branchiostoma floridae. However, the CT activity of Bf-CTFPs has yet to be investigated. In the present study, a functional analysis of Bf-CTFPs was performed using goldfish scales having both osteoclasts and osteoblasts...
January 3, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27917830/structural-analysis-of-the-bright-monomeric-yellow-green-fluorescent-protein-mneongreen-obtained-by-directed-evolution
#5
Damien Clavel, Guillaume Gotthard, David von Stetten, Daniele De Sanctis, Hélène Pasquier, Gerard G Lambert, Nathan C Shaner, Antoine Royant
Until recently, genes coding for homologues of the autofluorescent protein GFP had only been identified in marine organisms from the phyla Cnidaria and Arthropoda. New fluorescent-protein genes have now been found in the phylum Chordata, coding for particularly bright oligomeric fluorescent proteins such as the tetrameric yellow fluorescent protein lanYFP from Branchiostoma lanceolatum. A successful monomerization attempt led to the development of the bright yellow-green fluorescent protein mNeonGreen. The structures of lanYFP and mNeonGreen have been determined and compared in order to rationalize the directed evolution process leading from a bright, tetrameric to a still bright, monomeric fluorescent protein...
December 1, 2016: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/27418528/lanfp10-a-first-functional-fluorescent-protein-whose-chromophore-contains-the-elusive-mutation-g67a
#6
Abigail Roldán-Salgado, Celidee Sánchez-Barreto, Paul Gaytán
Since Green Fluorescent Protein (GFP) was first successfully expressed in heterologous systems in 1994, many genes encoding other natural autofluorescent proteins (AFPs) have been cloned and subsequently modified by protein engineering to improve their physicochemical properties. Throughout this twenty-two-year period, glycine 67 (Gly67) has been regarded as the only amino acid in the entire protein family that is essential for the formation of the different reported chromophores. In this work, we demonstrate that a synthetic gene encoding LanFP10-A, a natural protein encoded in the genome of the lancelet Branchiostoma floridae containing the G67A mutation, produces a heterologous, functional yellow fluorescent protein when expressed in E...
November 5, 2016: Gene
https://www.readbyqxmd.com/read/27412606/conserved-noncoding-elements-in-the-most-distant-genera-of-cephalochordates-the-goldilocks-principle
#7
Jia-Xing Yue, Iryna Kozmikova, Hiroki Ono, Carlos W Nossa, Zbynek Kozmik, Nicholas H Putnam, Jr-Kai Yu, Linda Z Holland
Cephalochordates, the sister group of vertebrates + tunicates, are evolving particularly slowly. Therefore, genome comparisons between two congeners of Branchiostoma revealed so many conserved noncoding elements (CNEs), that it was not clear how many are functional regulatory elements. To more effectively identify CNEs with potential regulatory functions, we compared noncoding sequences of genomes of the most phylogenetically distant cephalochordate genera, Asymmetron and Branchiostoma, which diverged approximately 120-160 million years ago...
2016: Genome Biology and Evolution
https://www.readbyqxmd.com/read/27311567/the-evolution-of-genes-encoding-for-green-fluorescent-proteins-insights-from-cephalochordates-amphioxus
#8
Jia-Xing Yue, Nicholas D Holland, Linda Z Holland, Dimitri D Deheyn
Green Fluorescent Protein (GFP) was originally found in cnidarians, and later in copepods and cephalochordates (amphioxus) (Branchiostoma spp). Here, we looked for GFP-encoding genes in Asymmetron, an early-diverged cephalochordate lineage, and found two such genes closely related to some of the Branchiostoma GFPs. Dim fluorescence was found throughout the body in adults of Asymmetron lucayanum, and, as in Branchiostoma floridae, was especially intense in the ripe ovaries. Spectra of the fluorescence were similar between Asymmetron and Branchiostoma...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27265651/expression-analysis-of-eight-amphioxus-genes-involved-in-the-wnt-%C3%AE-catenin-signaling-pathway
#9
Jing Wang, Guang Li, Guang-Hui Qian, Jun-Hao Hua, Yi-Quan Wang
The Wnt/β-catenin signaling pathway plays a crucial role in the embryonic development of metazoans. Although the pathway has been studied extensively in many model animals, its function in amphioxus, the most primitive chordate, remains largely uncharacterized. To obtain basic data for functional analysis, we identified and isolated seven genes (Lrp5/6, Dvl, APC, CkIα, CkIδ, Gsk3β, and Gro) of the Wnt/β-catenin signaling pathway from the amphioxus (Branchiostoma floridae) genome. Phylogenetic analysis revealed that amphioxus had fewer members of each gene family than that found in vertebrates...
May 18, 2016: Dong Wu Xue Yan Jiu, Zoological Research
https://www.readbyqxmd.com/read/26940763/tcf-lef-regulates-the-gsx-parahox-gene-in-central-nervous-system-development-in-chordates
#10
Myles G Garstang, Peter W Osborne, David E K Ferrier
BACKGROUND: The ParaHox genes play an integral role in the anterior-posterior (A-P) patterning of the nervous system and gut of most animals. The ParaHox cluster is an ideal system in which to study the evolution and regulation of developmental genes and gene clusters, as it displays similar regulatory phenomena to its sister cluster, the Hox cluster, but offers a much simpler system with only three genes. RESULTS: Using Ciona intestinalis transgenics, we isolated a regulatory element upstream of Branchiostoma floridae Gsx that drives expression within the central nervous system of Ciona embryos...
2016: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/26821753/structure-of-a-variable-lymphocyte-receptor-like-protein-from-the-amphioxus-branchiostoma-floridae
#11
Dong-Dong Cao, Xin Liao, Wang Cheng, Yong-Liang Jiang, Wen-Jie Wang, Qiong Li, Jun-Yuan Chen, Yuxing Chen, Cong-Zhao Zhou
Discovery of variable lymphocyte receptors (VLRs) in agnathans (jawless fish) has brought the origin of adaptive immunity system (AIS) forward to 500 million years ago accompanying with the emergence of vertebrates. Previous findings indicated that amphioxus, a representative model organism of chordate, also possesses some homologs of the basic components of TCR/BCR-based AIS, but it remains unknown if there exist any components of VLR-based AIS in amphioxus. Bioinformatics analyses revealed the amphioxus Branchiostoma floridae encodes a group of putative VLR-like proteins...
January 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/26796816/alpha-2-macroglobulin-is-a-maternally-derived-immune-factor-in-amphioxus-embryos-new-evidence-for-defense-roles-of-maternal-immune-components-in-invertebrate-chordate
#12
Anjalika Pathirana, Mingyue Diao, Shibo Huang, Lingling Zuo, Yujun Liang
In fish, a series of maternal derived immune components have been identified in their eggs or embryos at very early stages, which are proposed to provide protections to themselves against pathogenic attacks from hostile environment. The phenomenon of maternal immunity has been also recorded in several invertebrate species, however, so far, very limited information about the maternal immune molecules are available. In this study, it was demonstrated maternal alpha2 macroglobulin (A2m) protein, an important innate immune factor, exists in the fertilized eggs of amphioxus Branchiostoma japonicum, an invertebrate chordate...
March 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26747639/identification-and-characterization-of-an-apoptosis-stimulating-protein-of-p53-aspp-gene-from-branchiostoma-belcheri-insights-into-evolution-of-aspp-gene-family
#13
Xiaojun Song, Juan Du, Wei Zhu, Ping Jin, Fei Ma
The ASPP (apoptosis-stimulating protein of p53) protein family plays very key roles in apoptosis regulation, in both p53-dependent and p53-independent pathways. However, the ASPP homologous gene has not been identified in amphioxus to date. Here, we identified and characterized an ASPP gene from Branchiostoma belcheri (designed as AmphiASPP) and extensively studied its evolution and roles involved in innate immunity. The results showed that the amphioxus genome has an ASPP homolog gene with an ORF of 3285 bp, encoding 1094 amino acids which contains ANK repeats and SK3 domain...
February 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26680226/identification-of-a-novel-antimicrobial-peptide-from-amphioxus-branchiostoma-japonicum-by-in-silico-and-functional-analyses
#14
Haohan Liu, Miaomiao Lei, Xiaoyuan Du, Pengfei Cui, Shicui Zhang
The emergence of multi-drug resistant (MDR) microbes leads to urgent demands for novel antibiotics exploration. We demonstrated a cDNA from amphioxus Branchiostoma japonicum, designated Bjamp1, encoded a protein with features typical of antimicrobial peptides (AMPs), which is not homologous to any AMPs currently discovered. It was found that Bjamp1 was expressed in distinct tissues, and its expression was remarkably up-regulated following challenge with LPS and LTA. Moreover, the synthesized putative mature AMP, mBjAMP1, underwent a coil-to-helix transition in the presence of TFE or SDS, agreeing well with the expectation that BjAMP1 was a potential AMP...
December 18, 2015: Scientific Reports
https://www.readbyqxmd.com/read/26644465/evidence-for-conservation-of-the-calcitonin-superfamily-and-activity-regulating-mechanisms-in-the-basal-chordate-branchiostoma-floridae-insights-into-the-molecular-and-functional-evolution-in-chordates
#15
Toshio Sekiguchi, Kenji Kuwasako, Michio Ogasawara, Hiroki Takahashi, Shin Matsubara, Tomohiro Osugi, Ikunobu Muramatsu, Yuichi Sasayama, Nobuo Suzuki, Honoo Satake
The calcitonin (CT)/CT gene-related peptide (CGRP) family is conserved in vertebrates. The activities of this peptide family are regulated by a combination of two receptors, namely the calcitonin receptor (CTR) and the CTR-like receptor (CLR), and three receptor activity-modifying proteins (RAMPs). Furthermore, RAMPs act as escort proteins by translocating CLR to the cell membrane. Recently, CT/CGRP family peptides have been identified or inferred in several invertebrates. However, the molecular characteristics and relevant functions of the CTR/CLR and RAMPs in invertebrates remain unclear...
January 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26613046/on-the-origin-of-vertebrate-somites
#16
Takayuki Onai, Toshihiro Aramaki, Hidehiko Inomata, Tamami Hirai, Shigeru Kuratani
INTRODUCTION: Somites, blocks of mesoderm tissue located on either side of the neural tube in the developing vertebrate embryo, are derived from mesenchymal cells in the presomitic mesoderm (PSM) and are a defining characteristic of vertebrates. In vertebrates, the somite segmental boundary is determined by Notch signalling and the antagonistic relationship of the downstream targets of Notch, Lfng, and Delta1 in the anterior PSM. The presence of somites in the basal chordate amphioxus (Branchiostoma floridae) indicates that the last common ancestor of chordates also had somites...
2015: Zoological Letters
https://www.readbyqxmd.com/read/26573836/characterization-of-amphioxus-ifn-regulatory-factor-family-reveals-an-archaic-signaling-framework-for-innate-immune-response
#17
Shaochun Yuan, Tingting Zheng, Peiyi Li, Rirong Yang, Jie Ruan, Shengfeng Huang, Zhenxin Wu, Anlong Xu
The IFN regulatory factor (IRF) family encodes transcription factors that play important roles in immune defense, stress response, reproduction, development, and carcinogenesis. Although the origin of the IRF family has been dated back to multicellular organisms, invertebrate IRFs differ from vertebrate IRFs in genomic structure and gene synteny, and little is known about their functions. Through comparison of multiple amphioxus genomes, in this study we suggested that amphioxus contains nine IRF members, whose orthologs are supposed to be shared among three amphioxus species...
December 15, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/26479246/activity-augmentation-of-amphioxus-peptidoglycan-recognition-protein-bbtpgrp3-via-fusion-with-a-chitin-binding-domain
#18
Wen-Jie Wang, Wang Cheng, Ming Luo, Qingyu Yan, Hong-Mei Yu, Qiong Li, Dong-Dong Cao, Shengfeng Huang, Anlong Xu, Roy A Mariuzza, Yuxing Chen, Cong-Zhao Zhou
Peptidoglycan recognition proteins (PGRPs), which have been identified in most animals, are pattern recognition molecules that involve antimicrobial defense. Resulting from extraordinary expansion of innate immune genes, the amphioxus encodes many PGRPs of diverse functions. For instance, three isoforms of PGRP encoded by Branchiostoma belcheri tsingtauense, termed BbtPGRP1~3, are fused with a chitin binding domain (CBD) at the N-terminus. Here we report the 2.7 Å crystal structure of BbtPGRP3, revealing an overall structure of an N-terminal hevein-like CBD followed by a catalytic PGRP domain...
2015: PloS One
https://www.readbyqxmd.com/read/26442622/evolutionary-conservation-of-molecular-structure-and-antiviral-function-of-a-viral-receptor-lgp2-in-amphioxus-branchiostoma-japonicum
#19
Shousheng Liu, Yuanyuan Liu, Shuangshuang Yang, Youhua Huang, Qiwei Qin, Shicui Zhang
RIG-I-like (where RIG-I is retinoic acid inducible gene I) receptor LGP2 (where LGP2 is laboratory of genetics and physiology) is an important intracellular receptor that recognizes viral RNAs in innate immunity, but its origin and evolution remains unknown. Here we clearly demonstrate the presence of a RIG-I-like receptor, BjLGP2, in the basal chordate amphioxus. It is predominantly expressed in the hepatic caecum and hindgut, and is upregulated following challenge with poly(I:C). BjLGP2 is distributed in the cytoplasm of both grouper spleen and flounder gill (FG) cells, and the recombinant BjLGP2 interacts with poly(I:C)...
December 2015: European Journal of Immunology
https://www.readbyqxmd.com/read/26419477/molecular-signatures-that-are-distinctive-characteristics-of-the-vertebrates-and-chordates-and-supporting-a-grouping-of-vertebrates-with-the-tunicates
#20
Radhey S Gupta
Members of the phylum Chordata and the subphylum Vertebrata are presently distinguished solely on the basis of morphological characteristics. The relationship of the vertebrates to the two non-vertebrate chordate subphyla is also a subject of debate. Analyses of protein sequences have identified multiple conserved signature indels (CSIs) that are specific for Chordata or for Vertebrata. Five CSIs in 4 important proteins are specific for the Vertebrata, whereas two other CSIs are uniquely found in all sequenced chordate species including Ciona intestinalis and Oikapleura dioica (Tunicates) as well as Branchiostoma floridae (Cephalochordates)...
January 2016: Molecular Phylogenetics and Evolution
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