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ACAP protein

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https://www.readbyqxmd.com/read/27330119/acap3-regulates-neurite-outgrowth-through-its-gap-activity-specific-to-arf6%C3%A2-in-mouse-hippocampal-neurons
#1
Yuki Miura, Tsunaki Hongu, Yohei Yamauchi, Yuji Funakoshi, Naohiro Katagiri, Norihiko Ohbayashi, Yasunori Kanaho
ACAP3 (ArfGAP with coiled-coil, ankyrin repeat and pleckstrin homology domains 3) belongs to the ACAP family of GAPs (GTPase-activating proteins) for the small GTPase Arf (ADP-ribosylation factor). However, its specificity to Arf isoforms and physiological functions remain unclear. In the present study, we demonstrate that ACAP3 plays an important role in neurite outgrowth of mouse hippocampal neurons through its GAP activity specific to Arf6. In primary cultured mouse hippocampal neurons, knockdown of ACAP3 abrogated neurite outgrowth, which was rescued by ectopically expressed wild-type ACAP3, but not by its GAP activity-deficient mutant...
September 1, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27315435/roundup-%C3%A2-in-zebrafish-effects-on-oxidative-status-and-gene-expression
#2
Robson Rabelo Velasques, Juliana Zomer Sandrini, Carlos Eduardo da Rosa
The objective of the present study was to determine the effects of Roundup on oxidative status in adult Danio rerio liver and gills. Reactive oxygen species (ROS) and antioxidant capacity (ACAP) were measured in fish after exposure to Roundup (5 and 10 mg/L) for 24, 48, 72, and 96 h. Furthermore, gene expression related to antioxidant response was evaluated after 24 and 96 h. In gills, an increase in ACAP was observed after 96 h in the highest concentration. In the liver, a reduction in ROS and ACAP was observed after 24 h, whereas an increase in ACAP was observed after 48 h in the highest concentration...
October 2016: Zebrafish
https://www.readbyqxmd.com/read/26831792/-in-vitro-selected-macrocyclic-peptides-tools-for-regulating-the-conformational-freedom-of-transmembrane-proteins
#3
Christopher John Hipolito, Kosuke Nishio, Hiroaki Suga
Membrane proteins allow a cell to communicate with its environment by relaying a signal or transporting a molecule through the cell membrane. Elucidation of the three-dimensional structure of a membrane protein provides a greater understanding of its function and mechanisms. Ultimately, this knowledge will enlighten researchers on how these proteins can be regulated to elicit a desired cellular response, which could lead to novel therapeutic medicine. Unfortunately, the determination of the high-resolution crystal structures of transmembrane proteins remains a challenge due to their poor solubility and high conformational flexibility...
2016: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/26725004/effects-of-cryotherapy-combined-with-therapeutic-ultrasound-on-oxidative-stress-and-tissue-damage-after-musculoskeletal-contusion-in-rats
#4
C N Martins, M B Moraes, M Hauck, L F Guerreiro, D D Rossato, A S Varela, C E da Rosa, L U Signori
OBJECTIVE: To investigate the combined effects of cryotherapy and pulsed ultrasound therapy (PUT) on oxidative stress parameters, tissue damage markers and systemic inflammation after musculoskeletal injury. DESIGN: Experimental animal study. SETTING: Research laboratory. PARTICIPANTS: Seventy male Wistar rats were divided into five groups: control, lesion, cryotherapy, PUT, and cryotherapy+PUT. INTERVENTIONS: The gastrocnemius muscle was injured by mechanical crushing...
November 29, 2015: Physiotherapy
https://www.readbyqxmd.com/read/26615366/metal-contamination-as-a-possible-etiology-of-fibropapillomatosis-in-juvenile-female-green-sea-turtles-chelonia-mydas-from-the-southern-atlantic-ocean
#5
Cinthia Carneiro da Silva, Roberta Daniele Klein, Indianara Fernanda Barcarolli, Adalto Bianchini
Environmental contaminants have been suggested as a possible cause of fibropapillomatosis (FP) in green sea turtles. In turn, a reduced concentration of serum cholesterol has been indicated as a reliable biomarker of malignancy in vertebrates, including marine turtles. In the present study, metal (Ag, Cd, Cu, Fe, Ni, Pb and Zn) concentrations, oxidative stress parameters [antioxidant capacity against peroxyl radicals (ACAP), protein carbonyls (PC), lipid peroxidation (LPO), frequency of micronucleated cells (FMC)], water content, cholesterol concentration and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) activity were analyzed in the blood/serum of juvenile (29...
January 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/26037328/biochemical-responses-over-time-in-common-carp-cyprinus-carpio-teleostei-cyprinidae-during-fed-supplementation-with-%C3%AE-lipoic-acid
#6
Alain D Enamorado, Atila C Martins, Juliana A Flores, Marcelo Borges Tesser, Sergiane S Caldas, Ednei G Primel, José Maria Monserrat
The current study aimed to evaluate the influence of lipoic acid (LA) supplementation (439.84±6.71 mg LA/kg feed) on antioxidants responses throughout the time in intestine, liver and muscle of juvenile common carp Cyprinus carpio. Two experimental groups were fed during four weeks with a diet with or without LA. Glutathione-S-transferase (GST) activity, glutathione (GSH) content, antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (TBARS) were evaluated in these organs. Also, a technique to measure protein disulfide bonds and sulfhydryl groups was optimized for intestine samples...
October 2015: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/25988331/sorting-of-clathrin-independent-cargo-proteins-depends-on-rab35-delivered-by-clathrin-mediated-endocytosis
#7
Dipannita Dutta, Julie G Donaldson
Clathrin-mediated endocytosis (CME) and clathrin-independent endocytosis (CIE) co-exist in most cells but little is known about their communication and coordination. Here we show that when CME was inhibited, endocytosis by CIE continued but endosomal trafficking of CIE cargo proteins was altered. CIE cargo proteins that normally traffic directly into Arf6-associated tubules after internalization and avoid degradation (CD44, CD98 and CD147) now trafficked to lysosomes and were degraded. The endosomal tubules were also absent and Arf6-GTP levels were elevated...
September 2015: Traffic
https://www.readbyqxmd.com/read/25189617/defective-lysosome-maturation-and-legionella-pneumophila-replication-in-dictyostelium-cells-mutant-for-the-arf-gap-acap-a
#8
Nathalie Baïlo, Pierre Cosson, Steve J Charette, Valérie E Paquet, Patricia Doublet, François Letourneur
Dictyostelium discoideum ACAP-A is an Arf GTPase-activating protein (GAP) involved in cytokinesis, cell migration and actin cytoskeleton dynamics. In mammalian cells, ACAP family members regulate endocytic protein trafficking. Here, we explored the function of ACAP-A in the endocytic pathway of D. discoideum. In the absence of ACAP-A, the efficiency of fusion between post-lysosomes and the plasma membrane was reduced, resulting in the accumulation of post-lysosomes. Moreover, internalized fluid-phase markers showed extended intracellular transit times, and the transfer kinetics of phagocyted particles from lysosomes to post-lysosomes was reduced...
November 1, 2014: Journal of Cell Science
https://www.readbyqxmd.com/read/24996530/responses-of-biomarkers-in-wild-freshwater-mussels-chronically-exposed-to-complex-contaminant-mixtures
#9
Anderson Abel de S Machado, Chris M Wood, Adalto Bianchini, Patricia L Gillis
Subcellular biochemical biomarkers are valuable early warning indicators of environmental contaminant effects. Thus, the present study evaluated several biomarkers and the relationships among them in wild freshwater mussels (Lasmigona costata) from a gradient of metal exposure and differential levels of other urban-related influences in the Grand River (ON, Canada). The biomarkers examined are related to metal exposure [gill ion and metal concentrations (Na, K, Ca, Mg, Cd, Cu, Ni, Pb and Zn)], oxidative status [reactive oxygen species (ROS), catalase (CAT), superoxide dismutase (SOD), antioxidant capacity (ACAP)], sulfhydryl (SH) metabolism [glutathione (GSH), protein sulfhydryl groups (SH protein), glutathione S-transferase (GST), glutathione reductase (GR)], and lipid peroxidation...
September 2014: Ecotoxicology
https://www.readbyqxmd.com/read/24580823/evidence-of-oxidative-stress-in-wild-freshwater-mussels-lasmigona-costata-exposed-to-urban-derived-contaminants
#10
Patricia L Gillis, Sarah K Higgins, Marianna B Jorge
The physiological effect of complex mixtures of anthropogenic contaminants on aquatic organisms is not well understood. This study employed a suite of sub-cellular biomarkers and general health measurements to assess the effect of urban-derived contaminants on wild freshwater mussels. Adult Lasmigona costata were collected from four sites in the Grand River (ON, Canada) that receive incremental amounts of municipal wastewater effluents and road runoff. Biomarkers of metal exposure, oxidative stress, and general health were examined in the gills of wild mussels...
April 2014: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/23721848/biomarkers-of-waterborne-copper-exposure-in-the-guppy-poecilia-vivipara-acclimated-to-salt-water
#11
Anderson Abel de Souza Machado, Mariana Leivas Müller Hoff, Roberta Daniele Klein, Janaina Goulart Cardozo, Marina Mussoi Giacomin, Grasiela Lopes Leães Pinho, Adalto Bianchini
The responses of a large suite of biochemical and genetic parameters were evaluated in tissues (liver, gills, muscle and erythrocytes) of the estuarine guppy Poecilia vivipara exposed to waterborne copper in salt water (salinity 24 ppt). Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, and glutathione S-transferase), metallothionein-like protein concentration, reactive oxygen species (ROS) content, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO) were evaluated in liver, gills, and muscle...
August 15, 2013: Aquatic Toxicology
https://www.readbyqxmd.com/read/23264736/dictyostelium-acap-a-is-an-arfgap-involved-in-cytokinesis-cell-migration-and-actin-cytoskeleton-dynamics
#12
Marco Dias, Cédric Blanc, Nelcy Thazar-Poulot, Sabrina Ben Larbi, Pierre Cosson, François Letourneur
ACAPs and ASAPs are Arf-GTPase-activating proteins with BAR, PH, GAP and ankyrin repeat domains and are known to regulate vesicular traffic and actin cytoskeleton dynamics in mammalian cells. The amoeba Dictyostelium has only two proteins with this domain organization, instead of the six in human, enabling a more precise functional analysis. Genetic invalidation of acapA resulted in multinucleated cells with cytokinesis defects. Mutant acapA(-) cells were hardly motile and their multicellular development was significantly delayed...
February 1, 2013: Journal of Cell Science
https://www.readbyqxmd.com/read/20181831/h3-acidic-calponin-an-actin-binding-protein-that-controls-extracellular-signal-regulated-kinase-1-2-activity-in-nonmuscle-cells
#13
Sarah Appel, Philip G Allen, Susanne Vetterkind, Jian-Ping Jin, Kathleen G Morgan
Migration of fibroblasts is important in wound healing. Here, we demonstrate a role and a mechanism for h3/acidic calponin (aCaP, CNN3) in REF52.2 cell motility, a fibroblast line rich in actin filaments. We show that the actin-binding protein h3/acidic calponin associates with stress fibers in the absence of stimulation but is targeted to the cell cortex and podosome-like structures after stimulation with a phorbol ester, phorbol-12,13-dibutyrate (PDBu). By coimmunoprecipitation and colocalization, we show that extracellular signal-regulated kinase (ERK)1/2 and protein kinase C (PKC)alpha constitutively associate with h3/acidic calponin and are cotargeted with h3/acidic calponin in the presence of PDBu...
April 15, 2010: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/20062541/acap-a-b-are-arfgap-homologs-in-dictyostelium-involved-in-sporulation-but-not-in-chemotaxis
#14
Pei-Wen Chen, Paul A Randazzo, Carole A Parent
Arfs and Arf GTPase-activating proteins (ArfGAPs) are regulators of membrane trafficking and actin dynamics in mammalian cells. In this study, we identified a primordial Arf, ArfA, and two ArfGAPs (ACAP-A/B) containing BAR, PH, ArfGAP and Ankyrin repeat domains in the eukaryote Dictyostelium discoideum. In vitro, ArfA has similar nucleotide binding properties as mammalian Arfs and, with GTP bound, is a substrate for ACAP-A and B. We also investigated the physiological functions of ACAP-A/B by characterizing cells lacking both ACAP-A and B...
January 7, 2010: PloS One
https://www.readbyqxmd.com/read/19647779/effect-of-silymarin-on-biochemical-parameters-of-oxidative-stress-in-aged-and-young-rat-brain
#15
F Galhardi, K Mesquita, J M Monserrat, D M Barros
Silymarin (SM), the active complex of milk thistle, is a lipophilic fruit extract and is composed of several isomer flavonolignans. Flavonoids are antioxidants found molecules capable of intercepting reactive oxygen species (ROS). The oxidative stress (OS) is caused by imbalance between antioxidant defenses and production of ROS causing oxidative damage to macromolecules. Brain is susceptible to oxidative stress and it is associated with age-related brain dysfunction. This study evaluated the effect of SM on biochemical parameters that evaluate OS in aged and young rat brain...
October 2009: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/19363154/phosphoinositide-dependent-regulation-of-van3-arf-gap-localization-and-activity-essential-for-vascular-tissue-continuity-in-plants
#16
Satoshi Naramoto, Shinichiro Sawa, Koji Koizumi, Tomohiro Uemura, Takashi Ueda, Jírí Friml, Akihiko Nakano, Hiroo Fukuda
ACAP-type ARF GTPase activating proteins (ARF-GAPs) regulate multiple cellular processes, including endocytosis, secretion, phagocytosis, cell adhesion and cell migration. However, the regulation of ACAP functions by other cellular proteins is poorly understood. We have reported previously that a plant ACAP, VAN3, plays a pivotal role in plant venation continuity. Here, we report on newly identified VAN3 regulators: the CVP2 (cotyledon vascular pattern 2) 5 PTase, which is considered to degrade IP(3) and also to produce PtdIns(4)P from PtdIns(4,5)P(2); and a PH domain-containing protein, VAB (VAN3 binding protein)...
May 2009: Development
https://www.readbyqxmd.com/read/18849642/protease-activation-pancreatic-leakage-and-inflammation-in-acute-pancreatitis-differences-between-mild-and-severe-cases-and-changes-over-the-first-three-days
#17
S Regnér, J Manjer, S Appelros, C Hjalmarsson, J Sadic, A Borgström
BACKGROUND/AIMS: The pathophysiology of acute pancreatitis (AP) may be studied using markers of protease activation (active carboxypeptidase B (aCAP), the activation peptide of carboxypeptidase B (CAPAP)), leakage of pancreatic enzymes (trypsinogen-2, procarboxypeptidase B (proCAP), amylase), and inflammation (monocyte chemoattractant protein-1 (MCP-1), CRP). METHODS: This prospective study included 140 cases of AP. Mild (n = 124) and severe (n = 16) cases were compared with respect to serum levels of trypsinogen-2, proCAP, amylase, aCAP, CAPAP (serum/urine), MCP-1 (serum/urine) and CRP on days 1, 2 and 3 from onset of symptoms...
2008: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/18235216/monocyte-chemoattractant-protein-1-active-carboxypeptidase-b-and-capap-at-hospital-admission-are-predictive-markers-for-severe-acute-pancreatitis
#18
COMPARATIVE STUDY
S Regnér, S Appelros, C Hjalmarsson, J Manjer, J Sadic, A Borgstrom
BACKGROUND: CAPAP, the activation peptide of procarboxypeptidase B, is a predictor of severe acute pancreatitis (AP). Active carboxypeptidase (aCAP) may be a better predictor, as its turnover is slower. Monocyte chemotactic protein-1 (MCP-1) is an early inflammatory marker and increases before complications in severe AP. We conducted a cohort study to evaluate these markers as predictors for severe AP. METHOD: 140 patients with AP were included, retrospectively grouped as severe or mild by the Atlanta classification...
2008: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/16280360/an-effector-domain-mutant-of-arf6-implicates-phospholipase-d-in-endosomal-membrane-recycling
#19
Olivera A Jovanovic, Fraser D Brown, Julie G Donaldson
In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additionally, unlike wild-type Arf6, neither Arf6 mutant could generate protrusions or recruit the Arf6 GTPase activating protein (GAP) ACAP1 onto the endosome in the presence of aluminum fluoride. Remarkably, all of these phenotypes, including accumulated tubular endosomes, blocked recycling, and failure to make protrusions and recruit ACAP effectively, could be recreated in either untransfected cells or cells expressing wild-type Arf6 by treatment with 1-butanol to inhibit the formation of phosphatidic acid (PA), the product of PLD...
January 2006: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/16110251/active-carboxypeptidase-b-is-present-in-free-form-in-serum-from-patients-with-acute-pancreatitis
#20
Anders Borgström, Sara Regnér
BACKGROUND: Active carboxypeptidase B (aCAP) is the main product of activation of procarboxypeptidase. The other product derived from procarboxypeptidase B (proCAP), the activation peptide carboxypeptidase B (CAPAP), has been shown to be a marker for severity in acute pancreatitis. There have been relatively few studies dealing with the role of this enzyme in acute pancreatitis. METHODS: A double-antibody ELISA for immunoreactive aCAP (ir-aCAP) was developed, and ir-aCAP was determined in the serum of patients with acute pancreatitis and characterized using gel filtration...
2005: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
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