Vinod Kumar Reddy Lekkala, Soo-Yeon Kang, Jiafeng Liu, Sunil Shrestha, Prabha Acharya, Pranav Joshi, Mona Zolfaghar, Minseong Lee, Manav Goud Vanga, Paarth Jamdagneya, Sohan Pagnis, Arham Kundi, Samarth Kabbur, Ung Tae Kim, Yong Yang, Moo-Yeal Lee
Static three-dimensional (3D) cell culture has been demonstrated in ultralow attachment well plates, hanging droplet plates, and microtiter well plates with hydrogels or magnetic nanoparticles. Although it is simple, reproducible, and relatively inexpensive, thus potentially used for high-throughput screening, statically cultured 3D cells often suffer from a necrotic core due to limited nutrient and oxygen diffusion and waste removal and have a limited in vivo -like tissue structure. Here, we overcome these challenges by developing a pillar/perfusion plate platform and demonstrating high-throughput, dynamic 3D cell culture...
May 2, 2024: ACS Biomaterials Science & Engineering