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https://www.readbyqxmd.com/read/28343311/mechanistic-insight-of-platelet-apoptosis-leading-to-non-surgical-bleeding-among-heart-failure-patients-supported-by-continuous-flow-left-ventricular-assist-devices
#1
Nandan K Mondal, Tielou Li, Zengsheng Chen, Hegang H Chen, Erik N Sorensen, Si M Pham, Michael A Sobieski, Steven C Koenig, Mark S Slaughter, Bartley P Griffith, Zhongjun J Wu
Non-surgical bleeding (NSB) is the most common clinical complication in heart failure (HF) patients supported by continuous-flow left ventricular assist devices (CF-LVADs). In this study, oxidative stress and alteration of signal pathways leading to platelet apoptosis were investigated. Thirty-one HF patients supported by CF-LVADs were divided into bleeder (n = 12) and non-bleeder (n = 19) groups. Multiple blood samples were collected at pre-implant (baseline) and weekly up to 1-month post-implant. A single blood sample was collected from healthy subjects (reference)...
March 25, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28342833/a-high-density-cho-s-transient-transfection-system-comparison-of-expicho-and-expi293
#2
Nina K Jain, Susan Barkowski-Clark, Richard Altman, Krista Johnson, Fang Sun, Jonathan Zmuda, Chao Yan Liu, Adriana Kita, Ryan Schulz, Alyssa Neill, Robert Ballinger, Rekha Patel, Jian Liu, Alinafe Mpanda, Brian Huta, Henry Chiou, Walter Voegtli, Tadas Panavas
Chinese Hamster Ovary (CHO) cells are the principal mammalian host used for stable cell line generation and biotherapeutic protein production. Until recently, production of milligrams to grams of protein in CHO transient systems was challenging. As such, Human Embryonic Kidney (HEK293) cells are the most common mammalian cell type used for transient transfection. The post-translational modifications (PTMs) of a protein are dictated in part by the cell line used for expression, and changes in PTMs have been shown to affect both the activity and biophysical properties of proteins...
March 22, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28342668/the-herd-effects-of-infant-pcv7-pcv13-sequential-implementation-on-adult-invasive-pneumococcal-disease-six-years-post-implementation-a-nationwide-study-in-israel
#3
Gili Regev-Yochay, Michal Katzir, Jacob Strahilevitz, Galia Rahav, Talya Finn, Dan Miron, Yasmin Maor, Bibiana Chazan, Yehudith Schindler, Ron Dagan
BACKGROUND: Introduction of pneumococcal conjugate vaccine (PCV) has nearly eliminated vaccine-type (VT) invasive pneumococcal disease (IPD) in children, yet the reported resulting reduction of adult IPD is variable. We present the indirect impact of sequential PCV7/PCV13 implementation in Israel on adult IPD. METHODS: An ongoing nationwide active surveillance was initiated on July 2009 when PCV7 was implemented (with Catch-up). PCV7 was gradually replaced by PCV13 since November 2010...
March 22, 2017: Vaccine
https://www.readbyqxmd.com/read/28340069/the-association-of-post-traumatic-and-postmigration-stress-with-pain-and-other-somatic-symptoms-an-explorative-analysis-in-traumatized-refugees-and-asylum-seekers
#4
Naser Morina, Alexa Kuenburg, Ulrich Schnyder, Richard A Bryant, Angela Nickerson, Matthis Schick
Objective.:  Post-traumatic stress disorder (PTSD) and somatic symptoms, such as pain, are frequently seen in refugees. Their relationship is poorly understood, and the treatment of these comorbid conditions can be very challenging. The current cross-sectional study examined pain and other somatic symptoms and their relationship with trauma history, PTSD symptom clusters, and current living difficulties among treatment-seeking refugees. Methods.:  One hundred thirty-four treatment-seeking traumatized refugees (78% male, mean age = 42 years) were assessed regarding lifetime traumatic experiences, symptoms of post-traumatic stress, overall pain and somatic symptoms, and postmigration living difficulties...
March 6, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28339489/human-subtilisin-kexin-isozyme-1-ski-1-site-1-protease-s1p-regulates-cytoplasmic-lipid-droplet-abundance-a-potential-target-for-indirect-acting-anti-dengue-virus-agents
#5
Anastasia Hyrina, Fanrui Meng, Steven J McArthur, Sharlene Eivemark, Ivan R Nabi, François Jean
Viral hijacking and manipulation of host-cell biosynthetic pathways by human enveloped viruses are shared molecular events essential for the viral lifecycle. For Flaviviridae members such as hepatitis C virus and dengue virus (DENV), one of the key subsets of cellular pathways that undergo manipulation is the lipid metabolic pathways, underlining the importance of cellular lipids and, in particular, lipid droplets (LDs) in viral infection. Here, we hypothesize that targeting cellular enzymes that act as key regulators of lipid homeostasis and LD formation could represent a powerful approach to developing a novel class of broad-spectrum antivirals against infection associated with all DENV serotypes (1-4) circulating around the world...
2017: PloS One
https://www.readbyqxmd.com/read/28339457/targeted-aav5-smad7-gene-therapy-inhibits-corneal-scarring-in-vivo
#6
Suneel Gupta, Jason T Rodier, Ajay Sharma, Elizabeth A Giuliano, Prashant R Sinha, Nathan P Hesemann, Arkasubhra Ghosh, Rajiv R Mohan
Corneal scarring is due to aberrant activity of the transforming growth factor β (TGFβ) signaling pathway following traumatic, mechanical, infectious, or surgical injury. Altered TGFβ signaling cascade leads to downstream Smad (Suppressor of mothers against decapentaplegic) protein-mediated signaling events that regulate expression of extracellular matrix and myogenic proteins. These events lead to transdifferentiation of keratocytes into myofibroblasts through fibroblasts and often results in permanent corneal scarring...
2017: PloS One
https://www.readbyqxmd.com/read/28339416/improving-vocational-outcomes-in-first-episode-psychosis-by-addressing-cognitive-impairments-using-cognitive-adaptation-training
#7
Kelly A Allott, Eoin Killackey, Pamela Sun, Warrick J Brewer, Dawn I Velligan
BACKGROUND: Cognitive Adaptation Training (CAT) uses compensatory strategies and environmental supports to support cognitive impairments and improve functioning. CAT may be useful for addressing vocational recovery in first-episode psychosis (FEP) because cognitive impairments are common and vocational recovery is a key goal of young people with FEP. OBJECTIVE: To describe clinical observations and practice experience when delivering CAT with FEP clients and explore potential benefits via objective outcome measures for improving vocational outcomes...
March 23, 2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/28339077/paeoniflorin-attenuates-the-neuroinflammatory-response-in-a-rat-model-of-chronic-constriction-injury
#8
Jianyu Zhou, Jingxia Wang, Wei Li, Chenglong Wang, Li Wu, Jianjun Zhang
Neuropathic pain remains the most frequent cause of suffering and disability worldwide. Paeoniflorin (PF), a water‑soluble monoterpene glycoside extracted from the roots of Paeonia lactiflora Pall, has a wide range of pharmacological functions. Although the neuroprotective effect of PF has been reported in animal models of neuropathology, no systematic investigation has reported on the analgesic properties of PF in neuropathic pain. The aim of the present study was to investigate whether PF can alleviate neuropathic pain and to examine its possible mechanism...
March 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28338601/mycotoxin-biotransformation-by-native-and-commercial-enzymes-present-and-future-perspectives
#9
REVIEW
Martina Loi, Francesca Fanelli, Vania C Liuzzi, Antonio F Logrieco, Giuseppina Mulè
Worldwide mycotoxins contamination has a significant impact on animal and human health, and leads to economic losses accounted for billions of dollars annually. Since the application of pre- and post- harvest strategies, including chemical or physical removal, are not sufficiently effective, biological transformation is considered the most promising yet challenging approach to reduce mycotoxins accumulation. Although several microorganisms were reported to degrade mycotoxins, only a few enzymes have been identified, purified and characterized for this activity...
March 24, 2017: Toxins
https://www.readbyqxmd.com/read/28338566/midazolam-as-an-active-placebo-in-three-fentanyl-validated-nociceptive-pain-models
#10
Julian Prosenz, Burkhard Gustorff
The use of inactive placebos in early translational trials of potentially analgesic compounds is discouraged due to the side-effect profiles of centrally acting analgesics. Therefore, benzodiazepines are used, although their use has not been validated in this context. Whether benzodiazepines confound the results of acute pain tests is unknown. Midazolam (0.06 mg/kg) as an active placebo was investigated in three nociceptive models that included contact heat, electrical pain, and pressure pain thresholds in 24 healthy volunteers...
March 23, 2017: Pain
https://www.readbyqxmd.com/read/28338320/inhibitors-of-protein-methyltransferases-and-demethylases
#11
H Ümit Kaniskan, Michael L Martini, Jian Jin
Post-translational modifications of histones by protein methyltransferases (PMTs) and histone demethylases (KDMs) play an important role in the regulation of gene expression and transcription and are implicated in cancer and many other diseases. Many of these enzymes also target various nonhistone proteins impacting numerous crucial biological pathways. Given their key biological functions and implications in human diseases, there has been a growing interest in assessing these enzymes as potential therapeutic targets...
March 24, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28337794/muramyl-dipeptide-a-shared-structural-motif-of-peptidoglycans-is-a-novel-inducer-of-bone-formation-through-induction-of-runx2
#12
Ok-Jin Park, Jiseon Kim, Jihyun Yang, Cheol-Heui Yun, Seung Hyun Han
Peptidoglycan fragments released from gut microbiota can be delivered to the bone marrow and affect bone metabolism. Here, we investigated the regulation of bone metabolism by muramyl dipeptide (MDP), which is a shared structural unit of peptidoglycans. Increased bone and mineral density by enhanced bone formation were observed in mice administered with MDP. Remarkably, pre-treatment or post-treatment with MDP alleviated bone loss in RANKL-induced osteoporosis mouse models. MDP directly augmented osteoblast differentiation and bone-forming gene expression by Runx2 activation...
March 24, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28335690/promoting-physical-activity-after-stroke-via-self-management-a-feasibility-study
#13
Elisabeth Preston, Catherine M Dean, Louise Ada, Rosalyn Stanton, Sandy Brauer, Suzanne Kuys, Gordon Waddington
BACKGROUND: Many people with mild disability after stroke are physically inactive despite the risk of recurrent stroke. A self-management program may be one strategy to increase physical activity in stroke survivors. OBJECTIVES: To investigate the feasibility of a self-management program, and determine whether self-management can increase daily physical activity levels and self-efficacy for exercise, decrease cardiovascular risk, and improve walking ability, participation, and quality of life in people with mild disability after stroke...
March 23, 2017: Topics in Stroke Rehabilitation
https://www.readbyqxmd.com/read/28335684/bone-marrow-derived-endothelial-progenitor-cell-treatment-in-a-model-of-lateral-fluid-percussion-injury-in-rats-evaluation-of-acute-and-subacute-outcome-measures
#14
Eugene Park, Katya J Park, Elaine Liu, Rongcai Jiang, Jian-Ning Zhang, Andrew J Baker
Traumatic brain injury (TBI) continues to be a serious healthcare issue while therapies to treat TBI remain elusive. Promising results from the use of endothelial progenitor cells (EPCs) in numerous disease states highlight the pleiotropic capacity of this cell type. We have previously demonstrated that EPC conditioned media reduces axonal degeneration following in vitro OGD insult and concurrently improves white matter and microvascular outcome in vivo following midline fluid percussion injury. In the current study we evaluated the effectiveness of allogeneic endothelial cells derived from rat bone marrow on microvascular recovery and neuronal sparing following lateral fluid percussion injury...
March 23, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28335219/post-plasma-siox-coatings-of-metal-and-metal-oxide-nanoparticles-for-enhanced-thermal-stability-and-tunable-photoactivity-applications
#15
Patrick Post, Nicolas Jidenko, Alfred P Weber, Jean-Pascal Borra
The plasma-based aerosol process developed for the direct coating of particles in gases with silicon oxide in a continuous chemical vapor deposition (CVD) process is presented. It is shown that non-thermal plasma filaments induced in a dielectric barrier discharge (DBD) at atmospheric pressure trigger post-DBD gas phase reactions. DBD operating conditions are first scanned to produce ozone and dinitrogen pentoxide. In the selected conditions, these plasma species react with gaseous tetraethyl orthosilicate (TEOS) precursor downstream of the DBD...
May 13, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334904/genome-wide-mapping-of-infection-induced-sine-rnas-reveals-a-role-in-selective-mrna-export
#16
John Karijolich, Yang Zhao, Ravi Alla, Britt Glaunsinger
Short interspersed nuclear elements (SINEs) are retrotransposons evolutionarily derived from endogenous RNA Polymerase III RNAs. Though SINE elements have undergone exaptation into gene regulatory elements, how transcribed SINE RNA impacts transcriptional and post-transcriptional regulation is largely unknown. This is partly due to a lack of information regarding which of the loci have transcriptional potential. Here, we present an approach (short interspersed nuclear element sequencing, SINE-seq), which selectively profiles RNA Polymerase III-derived SINE RNA, thereby identifying transcriptionally active SINE loci...
March 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28334889/the-primary-transcriptome-of-neisseria-meningitidis-and-its-interaction-with-the-rna-chaperone-hfq
#17
Nadja Heidrich, Saskia Bauriedl, Lars Barquist, Lei Li, Christoph Schoen, Jörg Vogel
Neisseria meningitidis is a human commensal that can also cause life-threatening meningitis and septicemia. Despite growing evidence for RNA-based regulation in meningococci, their transcriptome structure and output of regulatory small RNAs (sRNAs) are incompletely understood. Using dRNA-seq, we have mapped at single-nucleotide resolution the primary transcriptome of N. meningitidis strain 8013. Annotation of 1625 transcriptional start sites defines transcription units for most protein-coding genes but also reveals a paucity of classical σ70-type promoters, suggesting the existence of activators that compensate for the lack of -35 consensus sequences in N...
March 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28334666/bio-refractory-dissolved-organic-matter-and-colorants-in-cassava-distillery-wastewater-characterization-coagulation-treatment-and-mechanisms
#18
Ming Zhang, Zhou Wang, Penghui Li, Hua Zhang, Li Xie
An important portion of organic matter and colorants still remain in the biologically treated distillery wastewater, leaving the dark brown and odorous downstream with the heavy loading of chemical oxygen demand and the potential of forming disinfection byproducts. However, those bio-recalcitrant colorants have not been clearly recognized. The current study investigated the features of the bio-refractory organic matter and colorants in a typical distillery effluent, cassava distillery wastewater; special attention was paid to their change and behaviors in the coagulation treatment following the bio-processes...
March 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28334014/the-quaternary-lidocaine-derivative-qx-314-in-combination-with-bupivacaine-for-long-lasting-nerve-block-efficacy-toxicity-and-the-optimal-formulation-in-rats
#19
Qinqin Yin, Jun Li, Qingshan Zheng, Xiaolin Yang, Rong Lv, Longxiang Ma, Jin Liu, Tao Zhu, Wensheng Zhang
OBJECTIVE: The quaternary lidocaine derivative (QX-314) in combination with bupivacaine can produce long-lasting nerve blocks in vivo, indicating potential clinical application. The aim of the study was to investigate the efficacy, safety, and the optimal formulation of this combination. METHODS: QX-314 and bupivacaine at different concentration ratios were injected in the vicinity of the sciatic nerve in rats; bupivacaine and saline served as controls (n = 6~10)...
2017: PloS One
https://www.readbyqxmd.com/read/28333288/exposure-efficacy-relationships-for-vedolizumab-induction-therapy-in-patients-with-ulcerative-colitis-or-crohn-s-disease
#20
Maria Rosario, Jonathan L French, Nathanael L Dirks, Serap Sankoh, Asit Parikh, Huyuan Yang, Silvio Danese, Jean-Frédéric Colombel, Michael Smyth, William J Sandborn, Brian G Feagan, Walter Reinisch, Bruce E Sands, Miguel Sans, Irving Fox
Background and Aims: A positive relationship between vedolizumab trough serum concentrations and clinical outcomes in patients with ulcerative colitis (UC) or Crohn's disease (CD) has been reported. Here we further explore exposure-efficacy relationships for vedolizumab induction therapy in post hoc analyses of GEMINI study data. Methods: Vedolizumab trough concentrations at Week 6 or 10 were grouped in quartiles and clinical outcome rates calculated. Exposure-efficacy relationships at Week 6 and potential baseline covariate effects were explored using logistic regression and individual predicted cumulative average concentration through Week 6 (Caverage) as exposure measure...
February 17, 2017: Journal of Crohn's & Colitis
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