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https://www.readbyqxmd.com/read/28941037/phosphatidylinositol-4-5-bisphosphate-targets-doc2b-to-the-plasma-membrane
#1
Lirin Michaeli, Irit Gottfried, Maria Bykhovskaia, Uri Ashery
DOC2B is a high-affinity Ca(2+) sensor that translocates from the cytosol to the plasma membrane (PM) and promotes vesicle priming and fusion. However, the molecular mechanism underlying its translocation and targeting to the PM in living cells is not completely understood. DOC2B interacts in vitro with the PM components phosphatidylserine, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2 ] and target SNAREs (t-SNAREs). Here we show that PI(4,5)P2 hydrolysis at the PM of living cells abolishes DOC2B translocation, whereas manipulations of t-SNAREs and other phosphoinositides have no effect...
September 22, 2017: Traffic
https://www.readbyqxmd.com/read/28940105/experimental-and-theoretical-studies-of-emodin-interacting-with-a-lipid-bilayer-of-dmpc
#2
REVIEW
Antonio R da Cunha, Evandro L Duarte, Hubert Stassen, M Teresa Lamy, Kaline Coutinho
Emodin is one of the most abundant anthraquinone derivatives found in nature. It is the active principle of some traditional herbal medicines with known biological activities. In this work, we combined experimental and theoretical studies to reveal information about location, orientation, interaction and perturbing effects of Emodin on lipid bilayers, where we have taken into account the neutral form of the Emodin (EMH) and its anionic/deprotonated form (EM(-)). Using both UV/Visible spectrophotometric techniques and molecular dynamics (MD) simulations, we showed that both EMH and EM(-) are located in a lipid membrane...
September 22, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28939906/pore-formation-in-lipid-membrane-i-continuous-reversible-trajectory-from-intact-bilayer-through-hydrophobic-defect-to-transversal-pore
#3
Sergey A Akimov, Pavel E Volynsky, Timur R Galimzyanov, Peter I Kuzmin, Konstantin V Pavlov, Oleg V Batishchev
Lipid membranes serve as effective barriers allowing cells to maintain internal composition differing from that of extracellular medium. Membrane permeation, both natural and artificial, can take place via appearance of transversal pores. The rearrangements of lipids leading to pore formation in the intact membrane are not yet understood in details. We applied continuum elasticity theory to obtain continuous trajectory of pore formation and closure, and analyzed molecular dynamics trajectories of pre-formed pore reseal...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28939853/rsv-glycoprotein-and-genomic-rna-dynamics-reveal-filament-assembly-prior-to-the-plasma-membrane
#4
Daryll Vanover, Daisy V Smith, Emmeline L Blanchard, Eric Alonas, Jonathan L Kirschman, Aaron W Lifland, Chiara Zurla, Philip J Santangelo
The human respiratory syncytial virus G protein plays an important role in the entry and assembly of filamentous virions. Here, we report the use of fluorescently labeled soybean agglutinin to selectively label the respiratory syncytial virus G protein in living cells without disrupting respiratory syncytial virus infectivity or filament formation and allowing for interrogations of respiratory syncytial virus virion assembly. Using this approach, we discovered that plasma membrane-bound respiratory syncytial virus G rapidly recycles from the membrane via clathrin-mediated endocytosis...
September 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28939366/membrane-induced-organization-and-dynamics-of-the-n-terminal-domain-of-chemokine-receptor-cxcr1-insights-from-atomistic-simulations
#5
Shalmali Kharche, Manali Joshi, Durba Sengupta, Amitabha Chattopadhyay
The CXC chemokine receptor 1 (CXCR1) is an important member of the G protein-coupled receptor (GPCR) family in which the extracellular N-terminal domain has been implicated in ligand binding and selectivity. The structure of this domain has not yet been elucidated due to its inherent dynamics, but experimental evidence points toward membrane-dependent organization and dynamics. To gain molecular insight into the interaction of the N-terminal domain with the membrane bilayer, we performed a series of microsecond time scale atomistic simulations of the N-terminal domain of CXCR1 in the presence and absence of POPC bilayers...
September 19, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28938568/senp3-grants-tight-junction-integrity-and-cytoskeleton-architecture-in-mouse-sertoli-cells
#6
Di Wu, Chun-Jie Huang, Faheem Ahmed Khan, Xiao-Fei Jiao, Xiao-Ming Liu, Nuruliarizki Shinta Pandupuspitasari, Rahim Dad Brohi, Li-Jun Huo
Germ cells develop in a sophisticated immune privileged microenvironment provided by specialized junctions contiguous the basement membrane of the adjacent Sertoli cells that constituted the blood-testis barrier (BTB) in seminiferous epithelium of testis in mammals. Deciphering the molecular regulatory machinery of BTB activity is central to improve male fertility and the role of post-translational modification including SUMOylation pathway is one of the key factors. Herein, we unveiled the mystery of the SUMO-2/3 specific protease SENP3 (Sentrin-specific protease 3) in BTB dynamics regulation...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28937994/modeling-of-annexin-a2-membrane-interactions-by-molecular-dynamics-simulations
#7
Davit Hakobyan, Volker Gerke, Andreas Heuer
The annexins are a family of Ca2+-regulated phospholipid binding proteins that are involved in membrane domain organization and membrane trafficking. Although they are widely studied and crystal structures are available for several soluble annexins their mode of membrane association has never been studied at the molecular level. Here we obtained molecular information on the annexin-membrane interaction that could serve as paradigm for the peripheral membrane association of cytosolic proteins by Molecular Dynamics simulations...
2017: PloS One
https://www.readbyqxmd.com/read/28936467/peroxisome-motility-measurement-and-quantification-assay
#8
Jeremy Metz, Inês G Castro, Michael Schrader
Organelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ROS homeostasis. They distribute uniformly in mammalian cells and move along microtubules via kinesin and dynein motors. Their metabolic cooperation with mitochondria and the endoplasmic reticulum (ER) is essential for the β-oxidation of fatty acids and the synthesis of myelin lipids and polyunsaturated fatty acids...
September 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28934328/the-human-vgf-derived-bioactive-peptide-tlqp-21-binds-heat-shock-71-kda-protein-8-hspa8-on-the-surface-of-sh-sy5y-cells
#9
Shamim Akhter, Sandipan Chakraborty, Daniela Moutinho, Elia Álvarez-Coiradas, Isaac Rosa, Juan Viñuela, Eduardo Domínguez, Angel García, Jesús R Requena
VGF (non-acronymic)is a secreted chromogranin/secretogranin that gives rise to a number of bioactive peptides by a complex proteolysis mechanism. VGF-derived peptides exert an extensive array of biological effects in energy metabolism, mood regulation, pain, gastric secretion function, reproduction and, perhaps, cancer. It is therefore surprising that very little is known about receptors and binding partners of VGF-derived peptides and their downstream molecular mechanisms of action. Here, using affinity chromatography and mass spectrometry-based protein identification, we have identified the heat shock cognate 71 kDa protein A8 (HSPA8)as a binding partner of human TLQP-21 on the surface of human neuroblastomaSH-SY5Y cells...
2017: PloS One
https://www.readbyqxmd.com/read/28933287/modulation-of-abc-transporters-by-nuclear-receptors-physiological-pathological-and-pharmacological-aspects
#10
Juan Pablo Rigalli, Guillermo Nicolás Tocchetti, Johanna Weiss
ABC transporters are membrane proteins mediating the efflux of endo- and xenobiotics. Transporter expression is not static but instead is subject to a dynamic modulation aiming at responding to changes in the internal environment and thus at maintaining homeostatic conditions. Nuclear receptors are ligand modulated transcription factors that get activated upon changes in the intracellular concentrations of the respective agonists and bind to response elements within the promoter of ABC transporters, thus modulating their expression and, consequently, their activity...
September 20, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28931899/energetics-and-mechanism-of-anion-permeation-across-formate-nitrite-transporters
#11
Kalina Atkovska, Jochen S Hub
Formate-nitrite transporters (FNTs) facilitate the translocation of monovalent polyatomic anions, such as formate and nitrite, across biological membranes. FNTs are widely distributed among pathogenic bacteria and eukaryotic parasites, but they lack human homologues, making them attractive drug targets. The mechanisms and energetics involved in anion permeation across the FNTs have remained largely unclear. Both, channel and transporter mode of function have been proposed, with strong indication of proton coupling to the permeation process...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931892/new-views-on-phototransduction-from-atomic-force-microscopy-and-single-molecule-force-spectroscopy-on-native-rods
#12
Sourav Maity, Nina Ilieva, Alessandro Laio, Vincent Torre, Monica Mazzolini
By combining atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS), we analyzed membrane proteins of the rod outer segments (OS). With this combined approach we were able to study the membrane proteins in their natural environment. In the plasma membrane we identified native cyclic nucleotide-gated (CNG) channels which are organized in single file strings. We also identified rhodopsin located both in the discs and in the plasma membrane. SMFS reveals strikingly different mechanical properties of rhodopsin unfolding in the two environments...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931032/a-microtubule-based-minimal-model-for-spontaneous-and-persistent-spherical-cell-polarity
#13
Panayiotis Foteinopoulos, Bela M Mulder
We propose a minimal model for the spontaneous and persistent generation of polarity in a spherical cell based on dynamic microtubules and a single mobile molecular component. This component, dubbed the polarity factor, binds to microtubules nucleated from a centrosome located in the center of the cell, is subsequently delivered to the cell membrane, where it diffuses until it unbinds. The only feedback mechanism we impose is that the residence time of the microtubules at the membrane increases with the local density of the polarity factor...
2017: PloS One
https://www.readbyqxmd.com/read/28930630/assessing-the-structure-and-stability-of-transmembrane-oligomeric-intermediates-of-an-%C3%AE-helical-toxin
#14
Rajat Desikan, Prabal K K Maiti, K Ganapathy Ayappa
Protein membrane interactions play an important role in our understanding of diverse phenomena ranging from membrane assisted protein aggregation to oligomerization and folding. Pore forming toxins (PFTs) form the primary vehicle for infection by several strains of bacteria. These proteins expressed in a water soluble form (monomers) bind to the target membrane (protomer) to form a transmembrane pore complex through a process of oligomerization. Based on the structure of the transmembrane domains, PFTs are broadly classified into β or α toxins...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28926699/effects-of-high-pressure-on-phospholipid-bilayers
#15
Wei Ding, Michail Palaiokostas, Ganesh Shahane, Wen Wang, Mario Orsi
The response of lipid membranes to changes in external pressure is important for many biological processes, and it can also be exploited for technological applications. In this work, we employ all-atom molecular dynamics simulations to characterize the changes in the physical properties of phospholipid bilayers brought about by high pressure (1000 bar). In particular, we study how the response differs, in relation to different chain unsaturation levels, by comparing mono-unsaturated 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and bi-unsaturated dioleoyl-phosphatidylcholine (DOPC) bilayers...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28926278/planar-optical-nano-antennas-resolve-cholesterol-dependent-nanoscale-heterogeneities-in-the-plasma-membrane-of-living-cells
#16
Raju Regmi, Pamina Martina Winkler, Valentin Flauraud, Kyra Borgman, Carlo Manzo, Juergen Brugger, Herve Rigneault, Jerome Wenger, Maria F Garcia-Parajo
Optical nano-antennas can efficiently confine light into nanoscopic hotspots enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform to investigate the dynamics of individual biomolecules in living cell membranes and their partitioning into cholesterol-dependent lipid nanodomains. Here, we present optical nano-antenna arrays with accessible surface hotspots to study the characteristic diffusion dynamics of phosphoethanolamine (PE) and sphingomyelin (SM) in the plasma membrane of living cells at the nanoscale...
September 19, 2017: Nano Letters
https://www.readbyqxmd.com/read/28926182/water-dynamics-and-proton-transport-mechanisms-of-nafion%C3%A2-117-phosphotungstic-acid-composite-membrane-a-molecular-dynamics-study
#17
Saeed Akbari, Mohammad Taghi Hamed Mosavian, Ali Ahmadpour, Fatemeh Moosavi
Nafion®/phosphotungstic acid composite membrane and impact of varying concentration of heteropoly acid (HPA) on the well-known effective mechanisms of proton transport were investigated using classical and quantum hopping molecular dynamics simulation. Our simulations demonstrated that the HPA particles exhibited a favorable influence on Grotthuss mechanism in proton transportation at low hydration levels. From radial distribution function examinations, it was found that HPA particles were solvated with water and also exhibited stronger affinity toward hydronium ions...
September 19, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28924664/two-color-single-molecule-tracking-in-live-cells
#18
Siegfried Hänselmann, Dirk-Peter Herten
Measuring the kinetics of protein-protein interactions between molecules in the plasma membrane of live cells provides valuable information for understanding dynamic processes, like cellular signaling, on a molecular scale. Two-color single-molecule tracking is a fluorescence microscopy-based method to detect and quantify specific protein-protein interactions on a single-event level, providing sensitivity to heterogeneities and rare events. Fundamentally, it allows following the movement of single molecules of two different protein species in live cells with a localization precision beyond the diffraction limit of light in real time...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28923919/multiple-interactions-between-an-arf-gef-complex-and-charged-lipids-determine-activation-kinetics-on-the-membrane
#19
Deepti Karandur, Agata Nawrotek, John Kuriyan, Jacqueline Cherfils
Lipidated small GTPases and their regulators need to bind to membranes to propagate actions in the cell, but an integrated understanding of how the lipid bilayer exerts its effect has remained elusive. Here we focused on ADP ribosylation factor (Arf) GTPases, which orchestrate a variety of regulatory functions in lipid and membrane trafficking, and their activation by the guanine-nucleotide exchange factor (GEF) Brag2, which controls integrin endocytosis and cell adhesion and is impaired in cancer and developmental diseases...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28921848/interconversion-rates-between-conformational-states-as-rationale-for-the-membrane-permeability-of-cyclosporines
#20
Jagna Witek, Max Mühlbauer, Bettina G Keller, Markus Blatter, Axel Meissner, Trixie Wagner, Sereina Riniker
Cyclic peptides have regained interest as potential inhibitors of challenging targets, but have often a low bioavailability. The natural product cyclosporine A (CsA) is the textbook exception. Despite its size and polar backbone it is able to passively cross membranes. This ability is hypothesized to be due to a conformational change from the low-energy conformation in water to a "congruent" conformation that is populated both in water and inside the membrane. Here, we use a combination of NMR measurements and kinetic models based on molecular dynamics simulations to rationalize the difference in the membrane permeability of cyclosporine E (CsE) and CsA...
September 17, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
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