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https://www.readbyqxmd.com/read/26194794/increased-efficiency-in-identifying-mixed-pollen-samples-by-meta-barcoding-with-a-dual-indexing-approach
#1
Wiebke Sickel, Markus J Ankenbrand, Gudrun Grimmer, Andrea Holzschuh, Stephan Härtel, Jonathan Lanzen, Ingolf Steffan-Dewenter, Alexander Keller
BACKGROUND: Meta-barcoding of mixed pollen samples constitutes a suitable alternative to conventional pollen identification via light microscopy. Current approaches however have limitations in practicability due to low sample throughput and/or inefficient processing methods, e.g. separate steps for amplification and sample indexing. RESULTS: We thus developed a new primer-adapter design for high throughput sequencing with the Illumina technology that remedies these issues...
2015: BMC Ecology
https://www.readbyqxmd.com/read/25670709/hemophagocytic-syndrome-in-the-setting-of-aids-and-disseminated-histoplasmosis-case-report-and-a-review-of-literature
#2
REVIEW
Anup Subedee, Nicholas Van Sickels
Hemophagocytic lymphohistiocytosis (HLH) is traditionally regarded as a rapidly progressive and often fatal illness. In patients with AIDS, HLH usually occurs secondary to opportunistic infections. Although popular guidelines exist for the diagnosis and management of HLH in general, no formal study has evaluated their applicability among adult patients who develop HLH in the setting of AIDS and opportunistic infections. The study reports on a case of HLH in a patient with AIDS and disseminated histoplasmosis...
September 2015: Journal of the International Association of Providers of AIDS Care
https://www.readbyqxmd.com/read/19666363/-treatment-of-sickle-cell-pain
#3
A A Diakite, Y Coulibaly, F Dicko-Traore, B Traore, B Togo, A Dembele, M Sylla, T Sidibe, M M Keita
OBJECTIVES: Evaluate the efficiency of the treatment of pain by analgesic according to World Health Organization (WHO). PATIENTS AND METHODS: We made a descriptive and analytic study in majors sickellers in pain crisis admitted in the service of paediatric of the hospital Gabriel Touré Bamako from January 2005 to January 2006. The efficiency of the treatment by painkiller was evaluated at second hour and at the 24 hour. RESULTS: Our study concerned 70 children with major of sickle cell of both sexes and aged from 6 to 15 years in pain crisis...
2009: Le Mali Médical
https://www.readbyqxmd.com/read/19250893/nanoscaled-periodic-surface-structures-of-medical-stainless-steel-and-their-effect-on-osteoblast-cells
#4
Patrick Elter, Franka Sickel, Andrea Ewald
Nanoscaled lamellar surface structures have been prepared on medical stainless steel AISI 316LVM surfaces by chemical etching of the decomposed phases. The effect of this structure on osteoblastic cells has been investigated. Long filopodia were developed by the cells perpendicular to the lamellar structure while almost no or only short filopodia were formed parallel to the lamellae. These results are explained in terms of a topographical influence of the nanostructure. During the growth process of the filopodia a nearly flat surface was recognized parallel to the lamellae while a topographical change was sensed perpendicular to the structure, which was preferred by the cells...
June 2009: Acta Biomaterialia
https://www.readbyqxmd.com/read/16487796/using-prealbumin-as-an-inflammatory-marker-for-patients-with-deep-space-infections-of-odontogenic-origin
#5
Larry L Cunningham, Matthew J Madsen, Joseph E Van Sickels
BACKGROUND: The general health of the host may contribute to the severity of odontogenic infections. An example is the link between protein malnutrition and host defense mechanisms, most notably cell-mediated and mucosal immune responses. The plasma concentration of prealbumin, a protein synthesized in the liver with a half-life of 1.8 days, is an indicator of malnutrition. MATERIALS AND METHODS: We retrospectively reviewed the medical records of all patients who were admitted to our hospital with a diagnosis of odontogenic infection in 2002 and 2003...
March 2006: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/15838665/a-ni-2-sensitive-component-of-the-erg-b-wave-from-the-isolated-bovine-retina-is-related-to-e-type-voltage-gated-ca-2-channels
#6
Matthias Lüke, Margit Henry, Thea Lingohr, Mehran Maghsoodian, Jürgen Hescheler, Marco Weiergräber, Werner Sickel, Toni Schneider
BACKGROUND: Voltage-dependent Ca(2+) channels trigger and control important cellular processes like neurotransmitter release and secretion, long-term potentiation, and gene expression in excitable cells. During retinal signal perception and processing, presynaptic Ca(2+) channels facilitate neurotransmitter release in photoreceptors and bipolar neurons, at nonspiking synapses which generate graded potentials. METHODS: The nature of voltage-gated Ca(2+) channels involved in retinal signal transduction is investigated in the present report by recording the electroretinogram (ERG) from the isolated and perfused bovine retina...
September 2005: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/13905130/-the-electroretinogram-in-man-in-the-absence-of-ganglionic-cells-and-in-partial-lesions-of-the-bipolar-cells
#7
W HASCHKE, W SICKEL
No abstract text is available yet for this article.
1962: Acta Ophthalmologica. Supplementum
https://www.readbyqxmd.com/read/13844489/a-comparative-study-of-renal-concentrating-ability-in-children-with-sickel-cell-anemia-and-in-normal-children
#8
C F WHITTEN, A A YOUNES
No abstract text is available yet for this article.
March 1960: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/13073083/sickel-cell-disease-in-pregnancy
#9
H G WINSTON, L MASTROIANNI
No abstract text is available yet for this article.
July 1953: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/11986952/expression-of-a-mutated-form-of-the-p85alpha-regulatory-subunit-of-phosphatidylinositol-3-kinase-in-a-hodgkin-s-lymphoma-derived-cell-line-co
#10
M Jücker, K Südel, S Horn, M Sickel, W Wegner, W Fiedler, R A Feldman
Phosphatidylinositol (PI) 3-kinase plays an important role in a variety of biological processes, including proliferation and apoptosis. PI3-kinase is a heterodimer consisting of an 85 kDa adapter protein (p85) containing one SH3 domain and two SH2 domains and a 110 kDa catalytic subunit (p110). Recently an oncogenic form of p85 named p65-PI3K lacking the C-terminal SH2 domain has been cloned from an irradiation-induced murine thymic lymphoma and transgenic mice expressing p65-PI3K in T lymphocytes develop a lymphoproliferative disorder...
May 2002: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/11324982/effects-of-protein-tyrosine-kinase-inhibitor-genistein-on-retinal-function-in-superfused-vertebrate-retina
#11
C Lüke, R Krott, M Lüke, J Lebek, P Walter, R Brunner, W Sickel
The aim of this study was to evaluate safe concentrations of genistein for a potential intraocular application using the isolated retina technique on bovine retina preparations. Bovine retinas were isolated and perfused with an oxygen pre-equilibrated standard solution. The electroretinogram (ERG) was recorded as a transretinal potential using silver/silver-chloride electrodes. After recording of stable ERG amplitudes, genistein was added to the solution in different concentrations. The percentage of b-wave reduction under the drug was calculated...
April 2001: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/11152194/squamous-cell-carcinoma-in-a-maxillary-odontogenic-keratocyst
#12
G J Makowski, S McGuff, J E Van Sickels
No abstract text is available yet for this article.
January 2001: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/10792577/calbindin-d28k-immunoreactivity-is-a-marker-for-a-subdivision-of-the-sexually-dimorphic-nucleus-of-the-preoptic-area-of-the-rat-developmental-profile-and-gonadal-steroid-modulation
#13
COMPARATIVE STUDY
M J Sickel, M M McCarthy
Calbindin-D28k (calbindin) is a 28 kilodalton calcium binding protein which potentially plays a role in neuroprotection. We report here the normal development and gonadal steroid modulation of a sexually dimorphic group of calbindin immunoreactive cells within the sexually dimorphic nucleus of the preoptic area (SDN) which we call the calbindin-immunoreactive SDN or CALB-SDN. Beginning on PN2, a faintly immunoreactive CALB-SDN is present, however, the volume is not sexually dimorphic. On PN4, the staining of the CALB-SDN appears more robust but the volume is still not sexually dimorphic...
May 2000: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/10645971/developmental-sex-differences-in-calbindin-d-28k-and-calretinin-immunoreactivity-in-the-neonatal-rat-hypothalamus
#14
D H Brager, M J Sickel, M M McCarthy
The proteins calbindin-D(28K) and calretinin buffer intracellular calcium and are speculated to be involved in the integration of neuronal signaling. Using Western blot analysis, we compared the levels of calbindin-D(28K) and calretinin in the developing male and female rat hypothalamus on postnatal days (PN) 0, PN2, PN4, PN6, PN8, and PN10. Analysis of variance (ANOVA) of mean calbindin levels indicated a significant effect of sex (p </=.001) and age (p </=. 0001) and a significant interaction (p </=...
February 15, 2000: Journal of Neurobiology
https://www.readbyqxmd.com/read/9862523/littoral-cell-angioma-of-the-spleen-an-additional-report-of-four-cases-with-emphasis-on-the-association-with-visceral-organ-cancers
#15
REVIEW
M Bisceglia, J Z Sickel, F Giangaspero, V Gomes, M Amini, M Michal
AIMS AND BACKGROUND: Littoral cell angioma (LCA) is an uncommon vascular tumor of the spleen recently described and interpreted as the tumoral counterpart of the normally present littoral cells lining the splenic sinus channels of red pulp. The diagnosis of LCA is suggested by a quite characteristic morphology and confirmed by the demonstration of a hybrid endothelial/histiocytic phenotype. METHODS: Four original and previously unreported cases of LCA are presented...
September 1998: Tumori
https://www.readbyqxmd.com/read/9856678/cd30-positive-anaplastic-large-cell-lymphoma-alcl-of-t-cell-lineage-in-a-14-month-old-infant-with-perinatally-acquired-hiv-1-infection
#16
A W Funkhouser, P J Katzman, J Z Sickel, J S Lambert
PURPOSE: CD30-positive anaplastic large cell lymphoma (ALCL) has been described in adults with HIV-1 infection but is extremely rare in HIV-1-infected infants and children. PATIENT AND METHODS: A 14-month-old girl with congenitally acquired HIV-1 infection presented with fever and a tender, erythematous, cystic mass on the right labium majorum. The mass was biopsied. Histologic examination and immunohistochemistry were performed. RESULTS: Histologic examination showed Touton-like giant cells resembling histiocytes and focally abundant neutrophils obscuring the lymphoid infiltrate...
November 1998: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/9498649/the-relationship-of-glycaemic-level-to-advanced-glycation-end-product-age-accumulation-and-retinal-pathology-in-the-spontaneous-diabetic-hamster
#17
COMPARATIVE STUDY
H P Hammes, B Wellensiek, I Klöting, E Sickel, R G Bretzel, M Brownlee
To assess the relationship between glucose and advanced glycation end products (AGE) and the relationship between AGE and retinal changes in vivo, we studied the time course of retinopathy over 12 months in trypsin digest preparations and measured glycaemia and retinal AGE in spontaneous diabetic hamsters of mild (MD) and severe (SD) phenotypes. Blood glucose levels were elevated in MD (9.44+/-0.76 mmol/l) and in SD (3 months: 24.3+/-1.4 mmol/l; 12 months: 31.7+/-0.8 mmol/l) over non-diabetic controls (NC: 7...
February 1998: Diabetologia
https://www.readbyqxmd.com/read/8982590/cytologic-features-of-deep-juvenile-xanthogranuloma
#18
R T Grenko, J Z Sickel, C S Abendroth, R E Cilley
Juvenile xanthogranuloma (JXG) is a rare lesion of the skin and deep tissues whose cytologic features have not previously been published. We report on 2 cases of JXG in patients aged 5 days and 5 yr who presented with an axillary mass and enlarged supraclavicular lymph node, respectively. Fine-needle aspiration and touch imprints made from the surgical biopsies yielded cellular specimens composed of histiocytes, multinucleated Touton-type giant cells, and variable admixtures of lymphocytes and eosinophils. One lesion was composed primarily of benign-appearing histiocytes with admixed multinucleated giant cells and a few lymphocytes...
November 1996: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/8688972/loss-of-glut2-glucose-transporter-expression-in-pancreatic-beta-cells-from-diabetic-chinese-hamsters
#19
COMPARATIVE STUDY
A Jörns, M Tiedge, E Sickel, S Lenzen
The diabetic Chinese hamster is a well-established animal model for NIDDM with a defective glucose-induced insulin secretory response. In the pancreas of nondiabetic hamsters, the GLUT2 glucose transporter was localized in the plasma membrane of insulin-positive beta cells. At variance with the rat, immunoreactivity was also detected in the cytoplasm. Other islet cell types were not GLUT2 positive. GLUT2 immunoreactivity was already significantly reduced in beta cells from mildly diabetic animals in spite of a normal insulin immunoreactivity...
June 1996: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/8609127/granular-histiocytosis-of-pelvic-lymph-nodes-following-total-hip-arthroplasty-the-presence-of-wear-debris-cytokine-production-and-immunologically-activated-macrophages
#20
D G Hicks, A R Judkins, J Z Sickel, R N Rosier, J E Puzas, R J O'Keefe
UNLABELLED: Infiltration of regional lymph nodes by macrophages has been demonstrated after total joint arthroplasty. Although lymph nodes regulate the immune response, neither cytokine production nor the degree of immunological activation of cells within these nodes after total joint arthroplasty has been investigated. Pelvic lymph nodes were obtained from five patients who had had a total of eleven arthroplasties in seven hips three to twenty years before a pelvic staging procedure for adenocarcinoma (of the prostate in four patients and of the endometrium in one)...
April 1996: Journal of Bone and Joint Surgery. American Volume
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