Paola Y Bertucci, A Silvina Nacht, Mariano Alló, Luciana Rocha-Viegas, Cecilia Ballaré, Daniel Soronellas, Giancarlo Castellano, Roser Zaurin, Alberto R Kornblihtt, Miguel Beato, Guillermo P Vicent, Adali Pecci
Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex...
July 2013: Nucleic Acids Research