Michael Pycraft Hughes, Krista S P Clarke, Rashedul Hoque, Oreoluwa V Griffiths, Emily J Kruchek, Federico Bertagna, Kamalan Jeevaratnam, Rebecca Lewis, Fatima H Labeed
The foundation of dielectrophoresis (DEP) as a tool for biological investigation is the use of the Clausius-Mossotti (C-M) factor to model the observed behaviour of cells experiencing DEP across a frequency range. Nevertheless, it is also the case that at lower frequencies, the DEP spectrum deviates from predictions; there exists a rise in DEP polarisability, which varies in frequency and magnitude with different cell types and medium conductivities. In order to evaluate the origin of this effect, we have studied DEP spectra from five cell types (erythrocytes, platelets, neurons, HeLa cancer cells and monocytes) in several conditions including medium conductivity and cell treatment...
January 9, 2024: Electrophoresis