Aanchal Mongia, Fatema Tuz Zohora, Noah G Burget, Yeqiao Zhou, Diane C Saunders, Yue J Wang, Marcela Brissova, Alvin C Powers, Klaus H Kaestner, Golnaz Vahedi, Ali Naji, Gregory W Schwartz, Robert B Faryabi
Cellular composition and anatomical organization influence normal and aberrant organ functions. Emerging spatial single-cell proteomic assays such as Image Mass Cytometry (IMC) and Co-Detection by Indexing (CODEX) have facilitated the study of cellular composition and organization by enabling high-throughput measurement of cells and their localization directly in intact tissues. However, annotation of cell types and quantification of their relative localization in tissues remain challenging. To address these unmet needs for atlas-scale datasets like Human Pancreas Analysis Program (HPAP), we develop AnnoSpat (Annotator and Spatial Pattern Finder) that uses neural network and point process algorithms to automatically identify cell types and quantify cell-cell proximity relationships...
May 3, 2024: Nature Communications