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https://www.readbyqxmd.com/read/29753148/sex-related-differences-in-the-associations-between-maternal-dioxin-like-compounds-and-reproductive-and-steroid-hormones-in-cord-blood-the-hokkaido-study
#1
Chihiro Miyashita, Atsuko Araki, Takahiko Mitsui, Sachiko Itoh, Houman Goudarzi, Seiko Sasaki, Jumboku Kajiwara, Tsuguhide Hori, Kazutoshi Cho, Kimihiko Moriya, Nobuo Shinohara, Katsuya Nonomura, Reiko Kishi
BACKGROUND: Prenatal exposure to dioxin-like compounds (DLCs) irreversibly affects fetal reproductive and steroid hormone synthesis. OBJECTIVE: This study aimed to assess the relationships between maternal DLCs and cord blood reproductive and steroid hormones. METHODS: Participants in this study were pregnant women who enrolled in the Sapporo Cohort of the Hokkaido Study between 2002 and 2005. We quantified 29 DLCs during the 2nd and 3rd trimesters in maternal blood...
May 9, 2018: Environment International
https://www.readbyqxmd.com/read/29740335/amniotic-fluid-insl3-measured-during-the-critical-time-window-in-human-pregnancy-relates-to-cryptorchidism-hypospadias-and-phthalate-load-a-large-case-control-study
#2
Ravinder Anand-Ivell, Arieh Cohen, Bent Nørgaard-Pedersen, Bo A G Jönsson, Jens-Peter Bonde, David M Hougaard, Christian H Lindh, Gunnar Toft, Morten S Lindhard, Richard Ivell
The period of the first to second trimester transition in human pregnancy represents a sensitive window for fetal organogenesis, particularly in regard to the development of the male reproductive system. This is a time of relative analytical inaccessibility. We have used a large national biobank of amniotic fluid samples collected at routine amniocentesis to determine the impacts of exogenous endocrine disruptor load on specific fetal biomarkers at this critical time. While adrenal and testicular steroids are highly correlated, they are also mostly positively influenced by increasing phthalate load, represented by the metabolites 7cx-MMeHP and 5cx-MEPP, by perfluorooctane sulfonate (PFOS) exposure, and by smoking, suggesting an adrenal stress response...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29674245/effects-of-multiple-life-stage-exposure-to-the-fungicide-prochloraz-in-xenopus-laevis-manifestations-of-antiandrogenic-and-other-modes-of-toxicity
#3
Jonathan T Haselman, Patricia A Kosian, Joseph J Korte, Allen W Olmstead, Sigmund J Degitz
The Larval Amphibian Growth and Development Assay (LAGDA) is an internationally harmonized testing guideline for evaluating effects of chronic chemical exposure in amphibians. In order to evaluate the effects of chronic exposure to an antiandrogenic chemical in an amphibian model, prochloraz was tested using a variation of the LAGDA design. Exposure was initiated with <1d post-fertilization embryos at nominal concentrations of 0, 6.7, 20, 60 and 180 μg/L (0, 18, 53, 159, 478 nM) and continued in flow-through conditions until two months following the median time that controls completed metamorphosis...
April 3, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29627606/gestational-exposure-to-ziram-disrupts-rat-fetal-leydig-cell-development
#4
Jianpeng Liu, Yiyan Wang, Yinghui Fang, Chaobo Ni, Leikai Ma, Wenwen Zheng, Suhao Bao, Xiaoheng Li, Qingquan Lian, Ren-Shan Ge
Ziram is an endocrine disruptor and may cause birth abnormality of the male reproductive system. However, the effects of ziram on fetal Leydig cell (FLC) development are still unknown. The objective of the present study was to determine the endocrine-disrupting effect of ziram on rat FLC development after gestational exposure. Pregnant Sprague Dawley dams were randomly divided into 5 groups and were gavaged with 0 (corn oil, the control), 1, 2, 4, or 8 mg/kg ziram from gestational day 12 (GD12) to GD21. FLC development was evaluated by measuring serum testosterone, FLC number and distribution, and the expression levels of Leydig and Sertoli cell genes...
March 21, 2018: Chemosphere
https://www.readbyqxmd.com/read/29627429/gestational-exposure-to-tetrabutyltin-blocks-rat-fetal-leydig-cell-development
#5
Yaoyao Dong, Yu Zhao, Qiqi Zhu, Zhe Wang, Yuanyuan Shan, Benson T Akingbemi, Ruijie Chen, Lihe Zhu, Ren-Shan Ge
Tetrabutyltin is a stable organotin and may exhibit endocrine disrupting properties. Herein, we investigated effects of tetrabutyltin on the development of rat fetal Leydig cells, which support differentiation of the male reproductive tract in late gestation. Female pregnant Sprague Dawley rats were gavaged with tetrabutyltin (0, 100, 200, and 500 mg/kg) from gestational day (GD) 12 to GD 21. Tetrabutyltin dose-dependently decreased testicular testosterone levels (0.756 ± 0.208 and 0.813 ± 0.277 ng/testis at the 200 and 500 mg/kg doses, respectively) compared to control (1...
April 5, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29615365/detection-of-insulin-like-growth-factor-receptor-1-in-the-human-cremaster-muscle-and-its-role-in-the-etiology-of-the-undescended-testis
#6
Mustafa Yaşar Özdamar, Sevinç Şahin, Kürşad Zengin, Selda Seçkin, Mesut Gürdal
PURPOSE: Previous studies of undescended testis (UT) has focused on insulin-like hormone 3 (INSL3), the genitofemoral nerve, and androgens in the testicular descent. Leydig cells, which are under the control of insulin-like growth factor-1 (IGF1), produce both androgens and INSL3. We aimed to investigate whether insulin-like growth factor receptor-1(IGFR1) exists in the cremaster muscle (CM) complex and is associated with normally descended testis as well as UT cases in humans. METHODS: We studied 30 CM from 15 patients who comprised the UT group (UTG), and 15 patients with unilateral testicular torsion (Control group; CG)...
March 31, 2018: Asian Journal of Surgery
https://www.readbyqxmd.com/read/29597124/plasma-igf-i-insl3-testosterone-inhibin-concentrations-and-scrotal-circumferences-surrounding-puberty-in-japanese-black-beef-bulls-with-normal-and-abnormal-semen
#7
W W P N Weerakoon, M Sakase, N Kawate, M A Hannan, N Kohama, H Tamada
The relationships between semen abnormalities and peripheral concentrations of testicular and metabolic hormones in beef bulls are unclear. Here we compared plasma insulin-like growth factor I (IGF-I), insulin-like peptide 3 (INSL3), testosterone, inhibin concentrations, and scrotal circumferences surrounding puberty in Japanese Black beef bulls (n = 66) with normal or abnormal semen. We collected blood samples and measured scrotal circumferences monthly from 4 to 24 months of age. Semen was collected weekly from 12 months until at least 18 months of age...
March 15, 2018: Theriogenology
https://www.readbyqxmd.com/read/29575065/evidence-for-the-role-of-insl3-on-sperm-production-in-boars-by-passive-immunisation
#8
I Minagawa, Y Murata, K Terada, M Shibata, E Y Park, H Sasada, T Kohsaka
Insulin-like factor 3 (INSL3), previously called relaxin-like factor, is essential for foetal testis descent and has been implicated in sperm production in adult males. This study investigated the role of INSL3 in sperm production by examining the effect of neutralising INSL3 by passive immunisation on testicular function and sperm output in boars. Six male Duroc boars were randomly assigned to passive immunisation and control groups (n = 3 each). The immunisation group was intravenously injected with an IgG fraction of anti-INSL3 antibody developed against the B domain of INSL3 at 2-week intervals from 21-40 weeks of age...
March 25, 2018: Andrologia
https://www.readbyqxmd.com/read/29476039/estrogen-induced-inhibition-of-spermatogenesis-in-zebrafish-is-largely-reversed-by-androgen
#9
Luiz Henrique de Castro Assis, Rafael Henrique de Nóbrega, Nuria Esther Gómez-González, Jan Bogerd, Rüdiger Winfried Schulz
The hormonal regulation of spermatogenesis involves both gonadotropins and steroid hormones. Long-term in vivo exposure of adult zebrafish to estrogen impaired spermatogenesis associated with an androgen insufficiency, possibly induced by inhibiting gonadotropin release. Using this experimental model, we investigated if androgen treatment could enhance spermatogenesis, while maintaining the inhibition of gonadotropin release through continued estrogen exposure. Moreover, we also exposed animals to androgen alone, in order to examine androgen effects in the absence of estrogen-induced gonadotropin inhibition...
May 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29452406/negative-association-between-sclerostin-and-insl3-in-isolated-human-osteocytes-and-in-klinefelter-syndrome-new-hints-for-testis-bone-crosstalk
#10
Andrea Di Nisio, Luca De Toni, Maria Santa Rocca, Marco Ghezzi, Riccardo Selice, Giuseppe Taglialavoro, Alberto Ferlin, Carlo Foresta
Context/Objective: The regulation of bone mass by the testis is a well-recognized mechanism, but the role of Leydig-specific marker insuline-like 3 peptide (INSL3) on the most abundant bone cell population, osteocytes, is unknown. In this study we aimed to investigate the relationship between INSL3 and Sclerostin, an osteocyte-specific protein that negatively regulates bone formation. Design/Setting: Serum sclerostin and INSL3 levels were evaluated in Klinefelter Syndrome (KS) and healthy controls...
February 14, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29430971/insulin-like-3-expression-and-fibrosis-induction-after-intra-testicular-injection-of-magnetic-nanoparticles-in-rat-testis-and-the-ameliorative-role-of-echinacea-purpurea-extract
#11
A Awaad, M A Adly, D Hosny
The World Health Organization has approved magnetic nanoparticles (MNP) for use as a contrast agent for magnetic resonance imaging or tumor hyperthermia treatment. MNP are toxic over time after intra-testicular injection. A clear strategy to ameliorate the toxic side effects of MNP in normal tissues after medical application has not yet been developed. We used an extract of Echinacea purpurea (EP) as a natural source of antioxidant and free radical scavenging product for detoxification of MNP in testicular tissues...
February 12, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29401475/overexpression-of-ubiquitin-carboxyl-terminal-hydrolase-1-uchl1-in-boys-with-cryptorchidism
#12
Dorota Toliczenko-Bernatowicz, Ewa Matuszczak, Marzena Tylicka, Beata Szymańska, Marta Komarowska, Ewa Gorodkiewicz, Wojciech Debek, Adam Hermanowicz
BACKGROUND: The ubiquitin-proteasome system regulate p53, caspase and Bcl-2 family proteins, and is crucial for the degradation of the defective germ cells in testes. Purpose: to evaluate the concentration of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in the blood plasma of boys with cryptorchidism and if there is any correlation with patient age. METHODS: Patients-50 boys aged 1-4 years (median = 2,4y.) with unilateral cryptorchidism. Exclusion criteria were: previous human chorionic gonadotropin treatment, an abnormal karyotype, endocrine or immunological disorders or any long-term medication...
2018: PloS One
https://www.readbyqxmd.com/read/29362488/transcriptional-regulation-of-p63-on-the-apoptosis-of-male-germ-cells-and-three-stages-of-spermatogenesis-in-mice
#13
Hong Wang, Qingqing Yuan, Minghui Niu, Wenhui Zhang, Liping Wen, Hongyong Fu, Fan Zhou, Zuping He
Infertility affects 10-15% of couples worldwide, and male factors account for 50%. Spermatogenesis is precisely regulated by genetic factors, and the mutations of genes result in abnormal spermatogenesis and eventual male infertility. The aim of this study was to explore the role and transcriptional regulation of P63 in the apoptosis and mouse spermatogenesis. P63 protein was decreased in male germ cells of P63(+/-) mice compared with wild-type mice. There was no obvious difference in testis weight, sperm motility, and fecundity between P63(+/-) and wild-type mice...
January 23, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29319231/temporal-and-spatial-expression-of-insulin-like-peptide-insl5a-and-insl5b-paralog-genes-during-the-embryogenesis-of-danio-rerio
#14
Massimo Venditti, Aldo Donizetti, Marcella Fiengo, Chiara Fasano, Alessandra Santillo, Francesco Aniello, Sergio Minucci
Relaxin (RLN) and insulin (INSL)-like peptides are member of the INSL/RLN superfamily, which are encoded by seven genes in humans and can activate the G-protein coupled receptor RXFP 1-4. These peptides evolved from a common ancestor, RLN3-like gene. Two rounds of whole genome duplication (WGD) in early vertebrate evolution, together with an additional WGD in the teleost lineage, caused an expansion of RLN genes set in the genome of Danio rerio. In particular, six RLN genes are present: a single copy of rln and insl3 genes, and two paralogs for the rln3 gene (rln3a and rln3b), and the insl5 gene (insl5a and insl5b)...
January 2018: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#15
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
March 1, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29229959/insulin-like-peptide-3-expressed-in-the-silkworm-possesses-intrinsic-disulfide-bonds-and-full-biological-activity
#16
Takatsugu Miyazaki, Masaaki Ishizaki, Hideo Dohra, Sungjo Park, Andre Terzic, Tatsuya Kato, Tetsuya Kohsaka, Enoch Y Park
Insulin-like peptide 3 (INSL3) is a member of the relaxin/insulin superfamily and is expressed in testicular Leydig cells. Essential for fetal testis descent, INSL3 has been implicated in testicular and sperm function in adult males via interaction with relaxin/insulin-like family peptide receptor 2 (RXFP2). The INSL3 is typically prepared using chemical synthesis or overexpression in Escherichia coli followed by oxidative refolding and proteolysis. Here, we expressed and purified full-length porcine INSL3 (pINSL3) using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29180110/interleukin-6-inhibits-the-differentiation-of-rat-stem-leydig-cells
#17
Yiyan Wang, Lanlan Chen, Lubin Xie, Linchao Li, Xiaoheng Li, Huitao Li, Jianpeng Liu, Xianwu Chen, Baiping Mao, Tiantian Song, Qingquan Lian, Ren-Shan Ge
Inflammation causes male hypogonadism. Several inflammatory cytokines, including interleukin 6 (IL-6), are released into the blood and may suppress Leydig cell development. The objective of the present study was to investigate whether IL-6 affected the proliferation and differentiation of rat stem Leydig cells. Leydig cell-depleted rat testis (in vivo) and seminiferous tubules (in vitro) with ethane dimethane sulfonate (EDS) were used to explore the effects of IL-6 on stem Leydig cell development. Intratesticular injection of IL-6 (10 and 100 ng/testis) from post-EDS day 14 to 28 blocked the regeneration of Leydig cells, as shown by the lower serum testosterone levels (21...
November 24, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29149730/divergence-of-insulin-superfamily-ligands-receptors-and-igf-binding-proteins-in-marine-versus-freshwater-stickleback-evidence-of-selection-in-known-and-novel-genes
#18
Tim Pellissier, Hend Al Nafea, Sara V Good
Three-spine stickleback (Gasterosteus aculeatus) is a teleost model for understanding genetic, physiological and morphological changes accompanying freshwater (FW) adaptation. There is growing evidence that the insulin superfamily plays important roles in traits involved in marine and FW adaptation. We performed a candidate gene analysis to look for evidence of selection on 33 insulin superfamily ligand-receptor genes and insulin-like growth factor binding proteins (Igfbp's) in stickleback. Using genotype data from 11 marine and 10 FW populations, we calculated the number of SNPs per site in regulatory and intronic regions, the number of synonymous and nonsynonymous mutations in coding regions, Wright's fixation index (Fst), and performed t-tests to identify SNPs with divergent genotype frequencies between marine/FW versus Atlantic/Pacific populations...
March 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29084059/insl3-expression-in-leydig-cell-hyperplasia-and-leydig-cell-tumors
#19
Nelli S Lakis, Kara A Lombardo, Shamlal Mangray, George J Netto, Daniela Salles, Andres Matoso
Insulin-like 3 (INSL3) is a hormone produced by Leydig cells (LCs) and leads to physiological testicular descent during embryonic development. We investigated the expression of INSL3 by immunohistochemistry in normal LCs, in Leydig cell tumor (LCT) (n=17 including 15 testes and 2 ovaries) and in Leydig cell hyperplasia (LCH) (n=10). Normally distributed LCs showed strong immunostaining in the cytoplasm in all cases. All 10 cases (100%) of LCH were strongly and diffusely positive in the intertubular areas. Six cases of LCH had nodules raging in size from 0...
October 27, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29028960/ovarian-follicular-theca-cell-recruitment-differentiation-and-impact-on-fertility-2017-update
#20
JoAnne S Richards, Yi A Ren, Nicholes Candelaria, Jaye E Adams, Aleksandar Rajkovic
The major goal of this review is to summarize recent exciting findings that have been published within the past 10 years that, to our knowledge, have not been presented in detail in previous reviews and that may impact altered follicular development in polycystic ovarian syndrome (PCOS) and premature ovarian failure in women. Specifically, we will cover the following: (1) mouse models that have led to discovery of the derivation of two precursor populations of theca cells in the embryonic gonad; (2) the key roles of the oocyte-derived factor growth differentiation factor 9 on the hedgehog (HH) signaling pathway and theca cell functions; and (3) the impact of the HH pathway on both the specification of theca endocrine cells and theca fibroblast and smooth muscle cells in developing follicles...
February 1, 2018: Endocrine Reviews
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