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Ya-Jie Lv, Yi Yang, Bing-Dong Sui, Cheng-Hu Hu, Pan Zhao, Li Liao, Ji Chen, Li-Qiang Zhang, Tong-Tao Yang, Shao-Feng Zhang, Yan Jin
Rational: Senescence of mesenchymal stem cells (MSCs) and the related functional decline of osteogenesis have emerged as the critical pathogenesis of osteoporosis in aging. Resveratrol (RESV), a small molecular compound that safely mimics the effects of dietary restriction, has been well documented to extend lifespan in lower organisms and improve health in aging rodents. However, whether RESV promotes function of senescent stem cells in alleviating age-related phenotypes remains largely unknown. Here, we intend to investigate whether RESV counteracts senescence-associated bone loss via osteogenic improvement of MSCs and the underlying mechanism...
2018: Theranostics
Kagaku Azuma, Qian Zhou, Kin-Ya Kubo
Although the understanding of the complex pathogenesis for osteoporosis is appreciable, the underlying mechanism is not yet fully elucidated. There is a great need to further characterize the available animal models in osteoporosis research. The senescence-accelerated mouse prone 6 (SAMP6) mice have been developed as the spontaneous experimental model for senile osteoporosis. Here, we provide a comprehensive overview of current research regarding the bone morphological and molecular alterations and the possible mechanisms involved in these changes...
April 4, 2018: Medical Molecular Morphology
Supatta Chawalitpong, Ratchanaporn Chokchaisiri, Apichart Suksamrarn, Shigeru Katayama, Takakazu Mitani, Soichiro Nakamura, Ahmad Ai Athamneh, Patcharee Ritprajak, Asada Leelahavanichkul, Ratchaneevan Aeimlapa, Narattaphol Charoenphandhu, Tanapat Palaga
Cyperenoic acid is a terpenoid isolated from the root of a medicinal plant Croton crassifolius with a wide range of biological activities. In this study, the effects of cyperenoic acid on osteoclast differentiation were investigated both in vitro and in vivo using receptor activator of nuclear factor-κB ligand (RANKL)-induced bone marrow-derived osteoclasts and senescence-accelerated mouse prone 6 (SAMP6). Cyperenoic acid significantly suppressed RANKL-induced osteoclast differentiation at the concentrations with no apparent cytotoxicity...
April 4, 2018: Scientific Reports
Tetsu Sugimura, Kenta Jounai, Konomi Ohshio, Hiroaki Suzuki, Takayoshi Kirisako, Yoshihiko Sugihara, Daisuke Fujiwara
The decline in immune function caused by aging increases the risk of infectious diseases, tumorigeneses and chronic inflammation, resulting in accelerating senescence. We previously reported a lactic acid bacteria, Lactococcus lactis strain Plasma (synonym of Lactococcus lactis subsp. lactis JCM 5805, Lc-Plasma), that stimulates plasmacytoid dendritic cells (pDCs), which play a crucial role in phylaxis from viral infection. In this study, we investigated the anti-aging effects of long-term oral administration of Lc-Plasma in a senescence-accelerated mouse strain, SAMP6...
May 2018: International Immunopharmacology
Lucia Dwi Antika, Eun-Jung Lee, Yun-Ho Kim, Min-Kyung Kang, Sin-Hye Park, Dong Yeon Kim, Hyeongjoo Oh, Yean-Jung Choi, Young-Hee Kang
Osteoporosis is one of the most prevalent forms of age-related bone diseases. Increased bone loss with advancing age has become a grave public health concern. This study examined whether phlorizin and phloretin, dihydrochalcones in apple peels, inhibited senile osteoporosis through enhancing osteoblastogenic bone formation in cell-based and aged mouse models. Submicromolar phloretin and phlorizin markedly stimulated osteoblast differentiation of MC3T3-E1 cells with increased transcription of Runx2 and osteocalcin...
November 2017: Journal of Nutritional Biochemistry
Xi Chen, Lihui Li, Jianmin Guo, Lingli Zhang, Yu Yuan, Binglin Chen, Zhongguang Sun, Jiake Xu, Jun Zou
This study examined the effects of different exercise intensities and durations on bone mineral density (BMD) and bone strength in senescence-accelerated mouse prone 6 (SAMP6) and determined the involvement of the Wnt signaling pathway in exercise-induced osteogenesis. Three-month-old male SAMP6 mice were randomly assigned to different speeds of treadmill running exercise representing low, medium and high intensity, with the duration of five and nine weeks, respectively. We showed that medium-intensity exercise had positive effects on skeletal health, including BMD and bone strength, and the efficacy was higher than that of low-intensity exercise...
November 1, 2016: Oncotarget
Bingdong Sui, Chenghu Hu, Li Liao, Yichen Chen, Xinyi Zhang, Xin Fu, Chenxi Zheng, Meng Li, Ling Wu, Xinyi Zhao, Yan Jin
Osteoporosis is caused by pathologic factors such as aging, hormone deficiency or excess, inflammation, and systemic diseases like diabetes. Bone marrow stromal cells (BMSCs), the mesenchymal progenitors for both osteoblasts and adipocytes, are modulated by niche signals. In differential pathologic states, the pathological characteristics of BMSCs to osteoporoses and functional differences are unknown. Here, we detected that trabecular bone loss co-existed with increased marrow adiposity in 6 osteoporotic models, respectively induced by natural aging, accelerated senescence (SAMP6), ovariectomy (OVX), type 1 diabetes (T1D), excessive glucocorticoids (GIOP) and orchidectomy (ORX)...
July 22, 2016: Scientific Reports
T Okiura, Y Oishi, A Takemura, A Ishihara
OBJECTIVES: Effects of collagen hydrolysate (CHD) on the oxidative capacity of the tibialis anterior muscle and the cortical and trabecular density of the femur were investigated in senescence-accelerated mouse prone 6 (SAMP6). METHODS: Sixteen-week-old male SAMP6 mice were divided into control (CON) and CHD groups. The CON group was given normal water, while the CHD group was given water containing CHD. Fibre cross-sectional areas (CSAs), fibre succinate dehydrogenase (SDH) staining intensity, and SDH activity of the tibialis anterior muscle were determined at 42 and 60 weeks of age...
June 1, 2016: Journal of Musculoskeletal & Neuronal Interactions
Yu-Hee Kim, Minhwa Park, Kyung-Ah Cho, Bo-Kyung Kim, Jung-Hwa Ryu, So-Youn Woo, Kyung-Ha Ryu
Osteoporosis is a disease that affects 35% women and 20% men aged more than 65 years. Reduction in bone formation and increased bone resorption are known factors that drive osteoporosis, but recent studies suggest a positive correlation between bone marrow adipose tissue (MAT) and osteoporosis. Previously, we have observed that tonsil-derived mesenchymal stem cells (T-MSCs) reduce MAT in a mouse model of bone marrow depletion. That prompted us to investigate on the senile osteoporosis to characterize the bone-forming effect, as well as MAT-reducing effect of T-MSCs...
August 1, 2016: Stem Cells and Development
Fei Wang, Yiming Wang, Yanlei Zhao, Qiping Zhan, Peng Yu, Jingfeng Wang, Changhu Xue
In the current study, ovariectomized (OVX) rats and the senescence-accelerated mouse strain P6 (SAMP6) were employed to establish models of postmenopausal osteoporosis and senile osteoporosis, respectively. The effects of treatment with sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP) on these two types of osteoporosis were investigated in vivo. Results showed that Ca-SGP significantly increased bone mineral density, ameliorated trabecular bone microstructure, and improved bone biomechanical properties in both OVX rats and SAMP6...
April 13, 2016: Journal of Agricultural and Food Chemistry
Chenxi Zheng, Bingdong Sui, Chenghu Hu, Yan Jin
OBJECTIVE: To investigate whether vitamin C can promote the proliferation ability of bone marrow mesenchymal stem cells (BMMSCs) derived from aging mice. METHODS: The senescence-accelerated mouse prone 6 (SAMP6) mice and senescence-accelerated mouse resistant 1 (SAMR1) mice were used as the test group and the control group, respectively, and the SAMP6 mice were examined by micro-CT to verify the senescent phenotype. BMMSCs were harvested from the two mouse lines and cultured in vitro, and the cells from SAMP6 mice were subjected to treatment with different concentrations of vitamin C...
December 2015: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Jinlong Sun, Leiguo Ming, Fengqing Shang, Lijuan Shen, Jihua Chen, Yan Jin
Because of the reduced potential for osteogenesis in aging bone marrow stromal cells, the balance of bone metabolism becomes disrupted, leading to various bone diseases. An increase in reactive oxygen species has been determined to be one of the key factors that accelerates the aging process in BMSCs. In these cells, increased expression of NADPH oxidases is the major source of ROS. In the current study, we suppressed the expression of NOX using apocynin, an effective antioxidant and free radical scavenger, and the results showed that aging BMSCs exhibited an enhanced potential for osteogenesis...
December 21, 2015: Scientific Reports
Hironobu Katsuyama, Shigeko Fushimi, Kunikazu Yamane, Yoko Watanabe, Koichiro Shimoya, Toshiko Okuyama, Midori Katsuyama, Kiyofumi Saijoh, Masafumi Tomita
Vitamin K2 (VK2) has been used as a therapeutic agent for osteoporosis, since it has been suggested to be able to reduce the frequency of fractures by improving bone quality; however, bone turnover is strictly regulated by various cytokines and hormones. In the present study, the effect of menaquinone-4 (MK-4) on bone turnover was investigated using the senescence-accelerated mouse prone 6 (SAMP6) strain. Since water-immersion restraint stress (WRS) causes a significant decrease in bone mineral density (BMD), WRS was used as the bone resorption model in the SAMP6 strain...
September 2015: Experimental and Therapeutic Medicine
Yufeng Zhang, Lu Wang, Feng Deng, Hongmei Qiu, Xiaohong Wu
PURPOSE: To evaluate differences in the spontaneous healing capacity of senescence-prone inbred strains (SAMP6) and senescence-resistant inbred strains (SAMR1) and determine the critical defect size in a mouse model of senescence-accelerated osteoporosis. METHODS: Unilateral full-thickness calvarial defects 2 or 4mm in diameter were made in 6-month-old male SAMP6 and SAMR1. Defects were evaluated in vivo by micro-CT at day 0 and 6 and 12 weeks postoperatively. Calvarial specimens were harvested at 12 weeks for hematoxylin and eosin staining, Masson's trichrome staining, and tartrate-resistant-acid-phosphatase (TRAP) staining...
July 2015: Archives of Gerontology and Geriatrics
Keiko Unno, Hiroyuki Yamamoto, Masateru Toda, Shiori Hagiwara, Kazuaki Iguchi, Minoru Hoshino, Fumiyo Takabayashi, Sanae Hasegawa-Ishii, Atsuyoshi Shimada, Masanori Hosokawa, Keiichi Higuchi, Masayuki Mori
The senescence-accelerated mouse prone10 (SAMP10) strain, a model of aging, exhibits cognitive impairments and cerebral atrophy. We noticed that SAMP10/TaSlc mice, a SAMP10 substrain, have developed persistent glucosuria over the past few years. In the present study, we characterized SAMP10/TaSlc mice and further identified a spontaneous mutation in the Slc5a2 gene encoding sodium-glucose co-transporter (SGLT) 2. The mean concentration of urine glucose was high in SAMP10/TaSlc mice and increased further with advancing age, whereas other strains of senescence-accelerated mice, including SAMP1/SkuSlc, SAMP6/TaSlc and SAMP8/TaSlc or normal aging control SAMR1/TaSlc mice, exhibited no detectable glucose in urine...
November 7, 2014: Biochemical and Biophysical Research Communications
Zuzana Saidak, Carole Le Henaff, Sofia Azzi, Caroline Marty, Pierre J Marie
Intermittent administration of parathyroid hormone (PTH) 1-34 at a standard dose has been shown to induce anabolic effects in bone. However, whether low-dose PTH promotes bone formation during senescence is unknown. To address this issue, we determined the effects of low-dose PTH and analysed the underlying mechanisms in prematurely senescent mice that display osteopenia. Treatment of 9-week-old Samp6 mice for 6 weeks with PTH at a standard dose (100 μg/kg per day) increased vertebral and femoral bone mass and improved bone microarchitecture as a result of increased bone-forming surfaces and mineral apposition rate (MAR)...
October 2014: Journal of Endocrinology
Noriko Ichinoseki-Sekine, Ryo Kakigi, Susumu Miura, Hisashi Naito
PURPOSE: Body weight in young growing and young adult animals was reduced by a high dietary density of whey protein concentrate; however, it is unclear whether dietary proteins similarly affect body weight in aging animals. Here, we examined whether whey protein or whey peptide ingestion suppressed body fat accumulation and affected protein expression and phosphorylation in skeletal muscle in aging mice. METHODS: Twenty-six male senescence-accelerated mouse prone 6 (SAMP6) mice were assigned randomly to three dietary treatment groups: 18...
June 2015: European Journal of Nutrition
Ali Mirsaidi, Konstantin Genelin, Jolanda R Vetsch, Scott Stanger, Felix Theiss, Richard A Lindtner, Brigitte von Rechenberg, Michael Blauth, Ralph Müller, Gisela A Kuhn, Sandra Hofmann Boss, Hannes L Ebner, Peter J Richards
Adipose-derived stromal cells (ASCs) are increasingly being used for orthopedic-based tissue engineering approaches due to their ability to readily undergo osteogenic differentiation. In the present study, we used in vitro and in vivo approaches to evaluate the use of ASCs as a treatment strategy for age-related osteoporosis. Molecular, histological and micro-computed tomography (micro-CT) based approaches confirmed that ASCs isolated from 18-week-old osteoporotic senescence-accelerated mice (SAMP6) were capable of undergoing osteogenesis when cultured in either silk fibroin (SF) scaffolds or scaffold-free microtissues (ASC-MT)...
August 2014: Biomaterials
Kimie Niimi, Eiki Takahashi
The senescence-accelerated mouse (SAM) is used as an animal model of senescence acceleration and age-associated disorders. SAM is derived from unexpected crosses between the AKR/J and unknown mouse strains. There are nine senescence-prone (SAMP) strains and three senescence-resistant (SAMR) strains. Although SAMP strains exhibit strain-specific and age-related pathological changes, the genes responsible for the pathologic changes in SAMP strains have not been comprehensively identified. In the present study, we evaluated sweet taste perception using the two-bottle test...
May 10, 2014: Physiology & Behavior
Julie Glowacki, Shuichi Mizuno, Jason Kung, Julie Goff, Michael Epperly, Tracy Dixon, Hong Wang, Joel S Greenberger
We tested the effects of mouse genotype (C57BL/6NHsd, NOD/SCID, SAMR1, and SAMP6) and ionizing irradiation on bone wound healing. Unicortical wounds were made in the proximal tibiae, and the time course of spontaneous healing and effects of irradiation were monitored radiographically and histologically. There was reproducible healing beginning with intramedullary osteogenesis, subsequent bone resorption by osteoclasts, gradual bridging of the cortical wound, and re-population of medullary hematopoietic cells...
March 2014: In Vivo
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