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https://www.readbyqxmd.com/read/28102936/unbiased-and-quantitative-proteomics-reveals-highly-increased-angiogenesis-induction-by-the-secretome-of-mesenchymal-stromal-cells-isolated-from-fetal-rather-than-adult-skin
#1
Massimiliano Gaetani, Cinzia Maria Chinnici, Anna Paola Carreca, Claudia Di Pasquale, Giandomenico Amico, Pier Giulio Conaldi
Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during post-natal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to post-natal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs)...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28096344/atrial-natriuretic-peptide-regulates-adipose-tissue-accumulation-in-adult-atria
#2
Nadine Suffee, Thomas Moore-Morris, Patrick Farahmand, Catherine Rücker-Martin, Gilles Dilanian, Magali Fradet, Daigo Sawaki, Geneviève Derumeaux, Pascal LePrince, Karine Clément, Isabelle Dugail, Michel Puceat, Stéphane N Hatem
The abundance of epicardial adipose tissue (EAT) is associated with atrial fibrillation (AF), the most frequent cardiac arrhythmia. However, both the origin and the factors involved in EAT expansion are unknown. Here, we found that adult human atrial epicardial cells were highly adipogenic through an epithelial-mesenchymal transition both in vitro and in vivo. In a genetic lineage tracing the WT1(CreERT2+/-)Rosa(tdT+/-) mouse model subjected to a high-fat diet, adipocytes of atrial EAT derived from a subset of epicardial progenitors...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28089076/comparative-analyses-of-secreted-proteins-in-plant-pathogenic-smut-fungi-and-related-basidiomycetes
#3
Mariana Schuster, Gabriel Schweizer, Regine Kahmann
In the ten years since the genome sequence of the basidiomycete corn smut fungus Ustilago maydis was published, additional genomes of smut species infecting different hosts became available. In addition, the genomes of related Malassezia species causing skin diseases and of Pseudozyma species not known to infect plants were determined. As secreted proteins are critical virulence determinants in U. maydis we compare here the secretomes of 12 basidiomycete species to gain information about their composition and conservation...
January 6, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28087659/plant-parasitic-nematodes-towards-understanding-molecular-players-in-stress-responses
#4
REVIEW
François-Xavier Gillet, Caroline Bournaud, Jose Dijair Antonino de Souza Júnior, Maria Fatima Grossi-de-Sa
BACKGROUND: Plant-parasitic nematode interactions occur within a vast molecular plant immunity network. Following initial contact with the host plant roots, plant-parasitic nematodes (PPNs) activate basal immune responses. Defence priming involves the release in the apoplast of toxic molecules derived from reactive species or secondary metabolism. In turn, PPNs must overcome the poisonous and stressful environment at the plant-nematode interface. The ability of PPNs to escape this first line of plant immunity is crucial and will determine its virulence...
January 13, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28087649/identification-of-unique-essential-proteins-from-a-m-tuberculosis-f15-lam4-kzn-phage-secretome-library
#5
Thamsanqa Emmanuel Chiliza, Manormoney Pillay, Balakrishna Pillay
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis disease (TB), the leading cause of death from bacterial infection worldwide. Although treatable, the resurgence of multi- and extensively-drug resistance TB is a major setback for the fight against TB globally. Consequently, there is an urgent need for new Mtb derived biomarkers for use in the design of new drugs and rapid point-of-care diagnostic or prognostic tools for management of TB transmission. Therefore, the present study aimed to identify unique Mtb secreted proteins from the extensively drug resistant Mtb F15/LAM4/KZN phage secretome library...
January 12, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28077297/exosomes-key-mediators-of-metastasis-and-pre-metastatic-niche-formation
#6
REVIEW
Richard J Lobb, Luize G Lima, Andreas Möller
While tumour cells are classically known to communicate via direct cell-to-cell contact and the secretion of soluble protein-based factors such as cytokines and growth factors, alternative novel mechanisms that promote tumour progression have recently emerged. Now, new critical components of the secretome thought to be involved in tumour progression are exosomes, small vesicles of endocytic origin that carry a variety of bioactive molecules, including proteins, lipids, RNA, as well as DNA molecules. Cancer cell-derived exosomes have been shown to participate in crucial steps of metastatic spread of a primary tumour, ranging from oncogenic reprogramming of malignant cells to formation of pre-metastatic niches...
January 8, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28070933/secretome-analysis-of-diarrhea-inducing-strains-of-escherichia-coli
#7
Raja Sekhar Nirujogi, Babylakshmi Muthusamy, Min-Sik Kim, Gajanan J Sathe, P T V Lakshmi, Olga N Kovbasnjuk, T S Keshava Prasad, Mary Wade, Rabih E Jabbour
Secreted proteins constitute a major part of virulence factors that are responsible for pathogenesis caused by gram negative bacteria. Enterohemorrhagic Escherichia coli (E. coli), EHEC O157:H7 is the major pathogen often causing outbreaks. However, studies have reported that the significant outbreaks caused by non O157:H7 E. coli strains, also known as "Big-Six" serogroup strains are increasing. There is no systematic study describing differential secreted proteins from these non-O157:H7 E. coli strains. In this study, we carried out mass spectrometry-based differential secretome analysis using tandem mass tags labeling strategy of non-O157:H7 E...
January 9, 2017: Proteomics
https://www.readbyqxmd.com/read/28063828/genome-wide-computational-analysis-of-the-secretome-of-brown-algae-phaeophyceae
#8
Makoto Terauchi, Takahiro Yamagishi, Takeaki Hanyuda, Hiroshi Kawai
Brown algae have evolved complex multicellularity in the heterokont lineage. They are phylogenetically distant to land plants, fungi and animals. Especially, the members of Laminariales (so-called kelps) have developed highly differentiated tissues. Extracellular matrix (ECM) plays pivotal roles in a number of essential processes in multicellular organisms, such as cell adhesion, cell and tissue differentiations, cell-to-cell communication, and responses to environmental stimuli. In these processes, a set of extracellular secreted proteins called the secretome operates remodeling of the physicochemical nature of ECM and signal transduction by interacting with cell surface proteins and signaling molecules...
January 4, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28061804/the-extracellular-proteome-of-two-bifidobacterium-species-reveals-different-adaptation-strategies-to-low-iron-conditions
#9
Pamela Vazquez-Gutierrez, Marc J A Stevens, Peter Gehrig, Simon Barkow-Oesterreicher, Christophe Lacroix, Christophe Chassard
BACKGROUND: Bifidobacteria are among the first anaerobic bacteria colonizing the gut. Bifidobacteria require iron for growth and their iron-sequestration mechanisms are important for their fitness and possibly inhibit enteropathogens. Here we used combined genomic and proteomic analyses to characterize adaptations to low iron conditions of B. kashiwanohense PV20-2 and B. pseudolongum PV8-2, 2 strains isolated from the feces of iron-deficient African infants and selected for their high iron-sequestering ability...
January 6, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28060763/comparison-of-the-tumor-cell-secretome-and-patient-sera-for-an-accurate-serum-based-diagnosis-of-pancreatic-ductal-adenocarcinoma
#10
Shakhawan Mustafa, Longqiang Pan, Aseel Marzoq, Malak Fawaz, Laureen Sander, Felix Rückert, Andrea Schrenk, Christina Hartl, Rico Uhler, Adem Yildirim, Oliver Strobel, Thilo Hackert, Nathalia Giese, Markus W Büchler, Jörg D Hoheisel, Mohamed Saiel Saeed Alhamdani
Pancreatic cancer is the currently most lethal malignancy. Toward an accurate diagnosis of the disease in body liquids, we studied the protein composition of the secretomes of 16 primary and established cell lines of pancreatic ductal adenocarcinoma (PDAC). Compared to the secretome of non-tumorous cells, 112 proteins exhibited significantly different abundances. Functionally, the proteins were associated with PDAC features, such as decreased apoptosis, better cell survival and immune cell regulation. The result was compared to profiles obtained from 164 serum samples from two independent cohorts - a training and a test set - of patients with PDAC or chronic pancreatitis and healthy donors...
January 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28053006/co-option-and-de-novo-gene-evolution-underlie-molluscan-shell-diversity
#11
Felipe Aguilera, Carmel McDougall, Bernard M Degnan
Molluscs fabricate shells of incredible diversity and complexity by localized secretions from the dorsal epithelium of the mantle. Although distantly-related molluscs express remarkably different secreted gene products, it remains unclear if the evolution of shell structure and pattern is underpinned by the differential co-option of conserved genes or the integration of lineage-specific genes into the mantle regulatory program. To address this, we compare the mantle transcriptomes of 11 bivalves and gastropods of varying relatedness...
January 4, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28045024/therapeutic-microparticles-functionalized-with-biomimetic-cardiac-stem-cell-membranes-and-secretome
#12
Junnan Tang, Deliang Shen, Thomas George Caranasos, Zegen Wang, Adam C Vandergriff, Tyler A Allen, Michael Taylor Hensley, Phuong-Uyen Dinh, Jhon Cores, Tao-Sheng Li, Jinying Zhang, Quancheng Kan, Ke Cheng
Stem cell therapy represents a promising strategy in regenerative medicine. However, cells need to be carefully preserved and processed before usage. In addition, cell transplantation carries immunogenicity and/or tumourigenicity risks. Mounting lines of evidence indicate that stem cells exert their beneficial effects mainly through secretion (of regenerative factors) and membrane-based cell-cell interaction with the injured cells. Here, we fabricate a synthetic cell-mimicking microparticle (CMMP) that recapitulates stem cell functions in tissue repair...
January 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/28035653/non-classical-monocytes-levels-increased-by-subcutaneous-fat-secretome-are-associated-with-less-rehospitalization-after-heart-failure-admission
#13
Sonia Eiras, Alfonso Varela-Román, Mariléia Cháves Andrade, Ana Castro, Rocío González-Ferreiro, Juan E Viñuela, Ángel Fernández-Trasancos, Marcos C Carreira, Ezequiel Álvarez, Felipe F Casanueva, José R González-Juanatey
Differential monocyte subsets are increased in obesity and heart failure (HF). We studied their role as predictors of rehospitalization for HF and their regulation by adipose tissue. Monocyte subsets and body fat composition were determined from 136 patients at the discharge after HF admission. Regulation of monocytes by SAT secretomes from obese/non-obese patients with HF was studied in a cell culture method. Proteomic analysis of secretome SAT was performed by LC-MALDI TOF/TOF. High CD14(-)CD16(+) monocyte levels indicated less rehospitalization for HF (p = 0...
December 29, 2016: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28024339/using-gold-nanoparticles-to-disrupt-the-tumor-microenvironment-an-emerging-therapeutic-strategy
#14
Jilian R Melamed, Rachel S Riley, Danielle M Valcourt, Emily S Day
Gold nanoparticles have received much attention recently as carriers for anticancer drugs and therapeutic oligonucleotides, but little research has investigated their potential to act as stand-alone therapeutics. Previous studies interrogating their short- and long-term systemic toxicity have found that although gold nanoparticles accumulate within and clear slowly from the liver and spleen, they do not appear to exert toxic effects in these organs. Interestingly, gold nanoparticles innately exhibit the ability to modulate the tumor microenvironment specifically by interfering with crosstalk between tumor cells and stromal cells...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/28019693/relaxation-of-dna-supercoiling-leads-to-increased-invasion-of-epithelial-cells-and-protein-secretion-by-campylobacter-jejuni
#15
Eoin Scanlan, Laura Ardill, Matthew V X Whelan, Claire Shortt, Jarlath E Nally, Billy Bourke, Tadhg Ó Cróinín
Invasion of intestinal epithelial cells by Campylobacter jejuni is a critical step during infection of the intestine by this important human pathogen. In this study we investigated the role played by DNA supercoiling in the regulation of invasion of epithelial cells and the mechanism by which this could be mediated. A significant correlation between more relaxed DNA supercoiling and an increased ability of C. jejuni strains to penetrate human epithelial cells was demonstrated. Directly inducing relaxation of DNA supercoiling in C...
December 26, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/28010772/characterization-of-clostridium-thermocellum-b8-secretome-and-purified-cellulosomes-for-lignocellulosic-biomass-degradation
#16
Karen O Osiro, Brenda R de Camargo, Rachel Satomi, Pedro Ricardo V Hamann, Jéssica Pinheiro Silva, Marcelo Valle de Sousa, Betania F Quirino, Elaine N Aquino, Carlos R Felix, André Melro Murad, Eliane F Noronha
The main goal of the present study was a complete proteomic characterization of total proteins eluted from residual substrate-bound proteins (RSBP), and cellulosomes secreted by Clostridium thermocellum B8 during growth in the presence of microcrystalline cellulose as a carbon source. The second goal was to evaluate their potential use as enzymatic blends for hydrolyzing agro-industrial residues to produce fermentable sugars. Protein identification through LC-MS/MS mass spectrometry showed that the RSBP sample, in addition to cellulosomal proteins, contains a wide variety of proteins, including those without a well-characterized role in plant cell wall degradation...
February 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28003119/secretomes-of-mycoplasma-hyopneumoniae-and-mycoplasma-flocculare-reveal-differences-associated-to-pathogenesis
#17
Jéssica A Paes, Karina R Lorenzatto, Sofia N de Moraes, Hercules Moura, John R Barr, Henrique B Ferreira
: Mycoplasma hyopneumoniae and Mycoplasma flocculare cohabit the porcine respiratory tract. However, M. hyopneumoniae causes the porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. Comparative analyses demonstrated high similarity between these species, which includes the sharing of all predicted virulence factors. Nevertheless, studies related to soluble secretomes of mycoplasmas were little known, although they are important for bacterial-host interactions. The aim of this study was to perform a comparative analysis between the soluble secreted proteins repertoires of the pathogenic Mycoplasma hyopneumoniae and its closely related commensal Mycoplasma flocculare...
December 18, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27995973/the-angiogenic-secretome-in-vegf-overexpressing-breast-cancer-xenografts
#18
Louis Dore-Savard, Esak Lee, Samata Kakkad, Aleksander S Popel, Zaver M Bhujwalla
The plasticity of cancer cells and the fluidity of the tumor microenvironment continue to present major challenges in the comprehensive understanding of cancer that is essential to design effective treatments. The tumor interstitial fluid (TIF) encompasses the secretome and holds the key to several of the phenotypic characteristics of cancer. Difficulties in sampling this fluid have resulted in limited characterization of its components. Here we have sampled TIF from triple negative and estrogen receptor (ER)-positive human breast tumor xenografts with or without VEGF overexpression...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27991726/an-optimized-version-of-the-secretome-protein-enrichment-with-click-sugars-specs-method-leads-to-enhanced-coverage-of-the-secretome
#19
Alperen Serdaroglu, Stephan A Müller, Ute Schepers, Stefan Bräse, Stefan F Lichtenthaler, Peer-Hendrik Kuhn
The secretome, the entirety of all soluble proteins either being secreted or proteolytically released by a cell, plays a key role in intercellular communication of multicellular organisms. Pathological alterations contribute to diseases such as hypertension, cancer, autoimmune disorders or neurodegenerative diseases. Hence, studying disease related perturbations of the secretome and the secretome itself covers an important aspect of cellular physiology. We recently developed the Secretome Protein Enrichment with Click Sugars (SPECS) method which enables the analysis of secretomes of in vitro cell cultures even in the presence of fetal calf serum with mass spectrometry...
December 19, 2016: Proteomics
https://www.readbyqxmd.com/read/27984345/vulvodynia-what-we-know-and-where-we-should-be-going
#20
Logan M Havemann, David R Cool, Pascal Gagneux, Michael P Markey, Jerome L Yaklic, Rose A Maxwell, Ashvin Iyer, Steven R Lindheim
OBJECTIVE: The aim of the study was to review the current nomenclature and literature examining microbiome cytokine, genomic, proteomic, and glycomic molecular biomarkers in identifying markers related to the understanding of the pathophysiology and diagnosis of vulvodynia (VVD). MATERIALS AND METHODS: Computerized searches of MEDLINE and PubMed were conducted focused on terminology, classification, and "omics" variations of VVD. Specific MESH terms used were VVD, vestibulodynia, metagenomics, vaginal fungi, cytokines, gene, protein, inflammation, glycomic, proteomic, secretomic, and genomic from 2001 to 2016...
December 15, 2016: Journal of Lower Genital Tract Disease
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