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https://www.readbyqxmd.com/read/29144439/cell-line-secretome-and-tumor-tissue-proteome-markers-for-early-detection-of-colorectal-cancer-a-systematic-review
#1
REVIEW
Megha Bhardwaj, Vanessa Erben, Petra Schrotz-King, Hermann Brenner
Objective: In order to find low abundant proteins secretome and tumor tissue proteome data have been explored in the last few years for the diagnosis of colorectal cancer (CRC). In this review we aim to summarize the results of studies evaluating markers derived from the secretome and tumor proteome for blood based detection of colorectal cancer. Methods: Observing the preferred reporting items for systematic reviews and meta-analysis (PRISMA) guidelines PubMed and Web of Science databases were searched systematically for relevant studies published up to 18 July 2017...
November 16, 2017: Cancers
https://www.readbyqxmd.com/read/29140428/hpv-negative-but-not-hpv-positive-oropharyngeal-carcinomas-induce-fibroblasts-to-support-tumour-invasion-through-micro-environmental-release-of-hgf-and-il-6
#2
Robert Bolt, Bernadette Foran, Craig Murdoch, Daniel W Lambert, Sally Thomas, Keith D Hunter
Human papillomavirus (HPV) infection is causally related to a subset of oropharyngeal carcinomas (OPC) and is linked to a more favourable prognosis compared to HPV-negative OPC. The mechanisms underlying this effect on prognosis are not fully understood, but interactions with the tumour microenvironment may be pivotal. Here, we investigated the role of the tumour microenvironment in HPV-positive compared to HPV-negative cancer using 2D and 3D modelling of OPC interactions with stromal fibroblasts. HPV-negative, but not HPV-positive, OPC-derived cell lines induced a rapid fibroblast secretory response that supported 2D cancer cell migration and invasion in vitro...
November 13, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/29135730/a-novel-secretome-biotherapeutic-influences-regeneration-in-critical-size-bone-defects
#3
Alexander J Burdette, Teja Guda, Michelle E Thompson, Richard Banas, Forest Sheppard
Severe traumatic injuries often result in critical size bone defects, which are unable to heal without treatment. Autologous grafting is the standard of care but requires additional surgeries for graft procurement. Amnion-derived multipotent progenitor cells release a secretome of biomolecules identified as integral to the process of bone regeneration and angiogenesis. This secretome is currently under development as a biotherapeutic. The efficacy of this secretome biotherapeutic was evaluated in vitro on the proliferation and migration of mesenchymal stem cells and osteoprogenitor cells as well as in vivo using a critical size rat calvarial defect model...
November 9, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29132205/secretome-of-aggregated-embryonic-stem-cell-derived-mesenchymal-stem-cell-modulates-the-release-of-inflammatory-factors-in-lipopolysaccharide-induced-peripheral-blood-mononuclear-cells
#4
Nastaran Mohammadi Ghahhari, Faezeh Maghsood, Saeed Jahandideh, Majid Lotfinia, Shirin Lak, Behrooz Johari, Asaad Azarnezhad, Mehdi Kadivar
Background: Bone marrow mesenchymal stem cells (BM-MSCs) have emerged as a potential therapy for various inflammatory diseases. Because of some limitations, several recent studies have suggested the use of embryonic stem cell-derived MSCs (ESC-MSCs) as an alternative for BM-MSCs. Some of the therapeutic effects of the ESC-MSCs are related to the secretion of a broad array of cytokines and growth factors, known as secretome. Harnessing this secretome for therapeutic applications requires the optimization of production of secretary molecules...
November 14, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29128905/down-syndrome-ipsc-derived-astrocytes-impair-neuronal-synaptogenesis-and-the-mtor-pathway-in-vitro
#5
Bruno H S Araujo, Carolini Kaid, Janaina S De Souza, Sérgio Gomes da Silva, Ernesto Goulart, Luiz C J Caires, Camila M Musso, Laila B Torres, Adriano Ferrasa, Roberto Herai, Mayana Zatz, Oswaldo K Okamoto, Esper A Cavalheiro
Several methods have been used to study the neuropathogenesis of Down syndrome (DS), such as mouse aneuploidies, post mortem human brains, and in vitro cell culture of neural progenitor cells. More recently, induced pluripotent stem cell (iPSC) technology has offered new approaches in investigation, providing a valuable tool for studying specific cell types affected by DS, especially neurons and astrocytes. Here, we investigated the role of astrocytes in DS developmental disease and the impact of the astrocyte secretome in neuron mTOR signaling and synapse formation using iPSC derived from DS and wild-type (WT) subjects...
November 11, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/29127410/ultrafiltration-combined-with-size-exclusion-chromatography-efficiently-isolates-extracellular-vesicles-from-cell-culture-media-for-compositional-and-functional-studies
#6
Birke J Benedikter, Freek G Bouwman, Tanja Vajen, Alexandra C A Heinzmann, Gert Grauls, Edwin C Mariman, Emiel F M Wouters, Paul H Savelkoul, Carmen Lopez-Iglesias, Rory R Koenen, Gernot G U Rohde, Frank R M Stassen
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies. Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29125293/oxidation-of-wine-polyphenols-by-secretomes-of-wild-botrytis-cinerea-strains-from-white-and-red-grape-varieties-and-determination-of-their-specific-laccase-activity
#7
Sabrina Zimdars, Julia Hitschler, Andreas Schieber, Fabian Weber
Processing of Botrytis cinerea infected grapes leads to enhanced enzymatic browning reactions mainly caused by the enzyme laccase which is able to oxidize a wide range of phenolic compounds. The extent of color deterioration depends on the activity of the enzymes secreted by the fungus. The present study revealed significant differences in the oxidative properties of secretomes of several B. cinerea strains isolated from five grape varieties. The presumably laccase containing secretomes varied in their catalytic activity toward six phenolic compounds present in grapes...
November 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29123962/distinctive-features-of-tumor-infiltrating-%C3%AE-%C3%AE-t-lymphocytes-in-human-colorectal-cancer
#8
S Meraviglia, E Lo Presti, M Tosolini, C La Mendola, V Orlando, M Todaro, V Catalano, G Stassi, G Cicero, S Vieni, J J Fourniè, F Dieli
γδ T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating γδ T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of γδ T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4% γδ T cells on average, with the majority expressing Vδ1...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/29119205/secretome-profile-analysis-of-multidrug-resistant-monodrug-resistant-and-drug-susceptible-mycobacterium-tuberculosis
#9
Chanyanuch Putim, Narumon Phaonakrop, Janthima Jaresitthikunchai, Ratikorn Gamngoen, Khajornsak Tragoolpua, Sorasak Intorasoot, Usanee Anukool, Chayada Sitthidet Tharincharoen, Ponrut Phunpae, Chatchai Tayapiwatana, Watchara Kasinrerk, Sittiruk Roytrakul, Bordin Butr-Indr
The emergence of drug-resistant tuberculosis has generated great concern in the control of tuberculosis and HIV/TB patients have established severe complications that are difficult to treat. Although, the gold standard of drug-susceptibility testing is highly accurate and efficient, it is time-consuming. Diagnostic biomarkers are, therefore, necessary in discriminating between infection from drug-resistant and drug-susceptible strains. One strategy that aids to effectively control tuberculosis is understanding the function of secreting proteins that mycobacteria use to manipulate the host cellular defenses...
November 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29118330/molecular-spectrum-of-secretome-regulates-the-relative-hepatogenic-potential-of-mesenchymal-stem-cells-from-bone-marrow-and-dental-tissue
#10
Ajay Kumar, Vinod Kumar, Vidya Rattan, Vivekananda Jha, Arnab Pal, Shalmoli Bhattacharyya
Liver regeneration is a spontaneous process that occurs after liver injury, but acute liver failure is a complex and fatal disease which is difficult to treat. Cell-based therapies are promising alternative therapeutic approach for liver failure and different cell sources have been tested in this regard. We investigated the comparative hepatogenic potential of human bone marrow stem cells (BMSC) with stem cells derived from human dental pulp (DPSC), apical papilla (SCAP) and follicle (DFSC) during this study...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115635/a-potential-role-for-hepcidin-in-obesity-driven-colorectal-tumourigenesis
#11
Elisabeth Phillips, Richard D Horniblow, Vikki Poole, Matthew Bedford, Douglas G Ward, Amanda J Kirkham, Jeremy Tomlinson, Tariq H Iqbal, Chris Tselepis
The obesity epidemic is associated with increases in the incidence of several types of cancer, including colorectal cancer, and is associated with poor outcomes for patients. Adipose tissue is considered biologically active and represents a plausible link between cancer and obesity due to the many factors that it secretes. In the present study, human adipose tissue was cultured in vitro and predifferentiated adipocyte secretome [preadipocyte (PAS)] and differentiated adipocyte secretome (DAS) were collected...
October 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29114223/the-human-amnion-epithelial-cell-secretome-decreases-hepatic-fibrosis-in-mice-with-chronic-liver-fibrosis
#12
Majid Alhomrani, Jeanne Correia, Marcus Zavou, Bryan Leaw, Nathan Kuk, Rong Xu, Mohamed I Saad, Alexander Hodge, David W Greening, Rebecca Lim, William Sievert
Background: Hepatic stellate cells (HSCs) are the primary collagen-secreting cells in the liver. While HSCs are the major cell type involved in the pathogenesis of liver fibrosis, hepatic macrophages also play an important role in mediating fibrogenesis and fibrosis resolution. Previously, we observed a reduction in HSC activation, proliferation, and collagen synthesis following exposure to human amnion epithelial cells (hAEC) and hAEC-conditioned media (hAEC-CM). This suggested that specific factors secreted by hAEC might be effective in ameliorating liver fibrosis...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29114066/senescence-and-aging-causes-consequences-and-therapeutic-avenues
#13
REVIEW
Domhnall McHugh, Jesús Gil
Aging is the major risk factor for cancer, cardiovascular disease, diabetes, and neurodegenerative disorders. Although we are far from understanding the biological basis of aging, research suggests that targeting the aging process itself could ameliorate many age-related pathologies. Senescence is a cellular response characterized by a stable growth arrest and other phenotypic alterations that include a proinflammatory secretome. Senescence plays roles in normal development, maintains tissue homeostasis, and limits tumor progression...
November 7, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29113965/mitochondrial-oxidative-stress-reduces-the-immunopotency-of-mesenchymal-stromal-cells-in-adults-with-coronary-artery-disease
#14
Ozge Kizilay Mancini, Maximilien Lora, Alexanne Cuillerier, Dominique Shum-Tim, Reggie C Hamdy, Yan Burelle, Marc J Servant, Ursula Stochaj, Ines Colmegna
Rationale: Mesenchymal stromal cells (MSCs) are promising therapeutic strategies for coronary artery disease (CAD), however, donor-related variability in cell quality is a main cause of discrepancies in preclinical studies. In vitro, MSCs from individuals with CAD have reduced ability to suppress activated T-cells. The mechanisms underlying the altered immunomodulatory capacity of MSCs in the context of atherosclerosis (ATH) remain elusive. Objective: The aim of this study was to assess the role of mitochondrial dysfunction in the impaired immunomodulatory properties of MSCs from ATH patients...
November 7, 2017: Circulation Research
https://www.readbyqxmd.com/read/29113460/impact-of-age-on-human-adipose-stem-cells-for-bone-tissue-engineering
#15
Denis Dufrane
Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy using mesenchymal stem cells (MSCs) have raised the possibility of implanting living tissue for bone reconstruction. Bone marrow was first proposed as the source of stem cells for bone regeneration...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29112105/secretome-conveys-the-protective-effects-of-ascs-therapeutic-potential-following-hemorrhagic-shock
#16
Mostafa Ashmwe, Carina Penzenstadler, Arian Bahrami, Anton Klotz, Mohammad Jafarmadar, Asmita Banerjee, Susanne Wolbank, Heinz Redl, Soheyl Bahrami
OBJECTIVES: We tested whether resuscitation supplemented with a) rat adipose-derived stem cells (ASCs) or b) secretome (conditioned media) of ASCs can ameliorate inflammation, cell/organ injury, and/or improve outcome after Hemorrhagic traumatic shock (HTS). INTERVENTIONS: Rats were subjected to HTS and a resuscitation protocol that mimics pre-hospital restrictive reperfusion followed by an adequate reperfusion phase. 20 minutes into the restrictive reperfusion, animals received an intravenous bolus of 2x10 cells (ASC group) or the secretome produced by 2x10 ASCs/24 h (ASC-Secretome group)...
November 3, 2017: Shock
https://www.readbyqxmd.com/read/29109537/neuroprotective-effect-of-ruminococcus-albus-on-oxidatively-stressed-sh-sy5y-cells-and-animals
#17
Jieun Park, Jiyun Lee, Zia Yeom, Donghyuk Heo, Young-Hee Lim
Recent evidence shows that the gut microbiota has an important role in gut-brain crosstalk and is linked to neuronal disorders. The aim of this study was to investigate the effects of intestinal Ruminococcus albus with probiotic potential on neuroprotection in oxidatively stressed SH-SY5Y neuroblastoma cells and animals. To investigate these effects, conditioned medium was prepared using Caco-2 cells cultured with heat-killed R. albus (CRA-CM). Caco-2 cells cultured with heat-killed R. albus showed increased BDNF expression and BDNF protein levels increased in CRA-CM...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29109463/a-role-for-small-secreted-proteins-ssps-in-a-saprophytic-fungal-lifestyle-ligninolytic-enzyme-regulation-in-pleurotus-ostreatus
#18
Daria Feldman, David J Kowbel, N Louise Glass, Oded Yarden, Yitzhak Hadar
Small secreted proteins (SSPs), along with lignocellulose degrading enzymes, are integral components of the secretome of Pleurotus ostreatus, a white rot fungus. In this study, we identified 3 genes (ssp1, 2 and 3) encoding proteins that are annotated as SSPs and that exhibited of ~4,500- fold expression, 24 hr following exposure to the toxic compound 5-hydroxymethylfurfural (HMF). Homologues to genes encoding these SSPs are present in the genomes of other basidiomycete fungi, however the role of SSPs is not yet understood...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29109100/high-resolution-proteomic-analysis-of-the-cervical-cancer-cell-lines-secretome-documents-deregulation-of-multiple-proteases
#19
REVIEW
Kalliopi I Pappa, Georgia Kontostathi, Manousos Makridakis, Vasiliki Lygirou, Jerome Zoidakis, George Daskalakis, Nicholas P Anagnou
BACKGROUND: Oncogenic infection by HPV, eventually leads to cervical carcinogenesis, associated by deregulation of specific pathways and protein expression at the intracellular and secretome level. Thus, secretome analysis can elucidate the biological mechanisms contributing to cervical cancer. In the present study we systematically analyzed its constitution in four cervical cell lines employing a highly sensitive proteomic technology coupled with bioinformatics analysis. MATERIALS AND METHODS: LC/MS-MS proteomics and bioinformatics analysis were performed in the secretome of four informative cervical cell lines SiHa (HPV16(+)), HeLa (HPV18(+)), C33A (HPV(-)) and HCK1T (normal)...
November 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/29106524/cardiac-myocyte-%C3%AE-3-adrenergic-receptors-prevent-myocardial-fibrosis-by-modulating-oxidant-stress-dependent-paracrine-signaling
#20
Nerea Hermida, Lauriane Michel, Hrag Esfahani, Emilie Dubois-Deruy, Joanna Hammond, Caroline Bouzin, Andreas Markl, Henri Colin, Anne Van Steenbergen, Christophe De Meester, Christophe Beauloye, Sandrine Horman, Xiaoke Yin, Manuel Mayr, Jean-Luc Balligand
Aims: Human and mouse cardiac beta3-adrenergic receptors (beta3AR) exert antipathetic effects to those of beta1-2AR stimulation. We examined their role in modulating myocardial remodelling, particularly fibrosis in response to haemodynamic stress. Methods and results: Mice with cardiac myocyte-specific expression of beta3AR (ADRB3-tg) or tamoxifen-inducible homozygous deletion (c-Adrb3-ko, with loxP-targeted Adrb3) were submitted to transaortic constriction. A superfusion assay was used for proteomic analysis of paracrine mediators between beta3AR-expressing cardiac myocytes and cardiac fibroblasts cultured separately...
July 6, 2017: European Heart Journal
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