keyword
MENU ▼
Read by QxMD icon Read
search

Secretome

keyword
https://www.readbyqxmd.com/read/27926865/tenascin-c-orchestrates-glioblastoma-angiogenesis-by-modulation-of-pro-and-anti-angiogenic-signaling
#1
Tristan Rupp, Benoit Langlois, Maria M Koczorowska, Agata Radwanska, Zhen Sun, Thomas Hussenet, Olivier Lefebvre, Devadarssen Murdamoothoo, Christiane Arnold, Annick Klein, Martin L Biniossek, Vincent Hyenne, Elise Naudin, Ines Velazquez-Quesada, Oliver Schilling, Ellen Van Obberghen-Schilling, Gertraud Orend
High expression of the extracellular matrix component tenascin-C in the tumor microenvironment correlates with decreased patient survival. Tenascin-C promotes cancer progression and a disrupted tumor vasculature through an unclear mechanism. Here, we examine the angiomodulatory role of tenascin-C. We find that direct contact of endothelial cells with tenascin-C disrupts actin polymerization, resulting in cytoplasmic retention of the transcriptional coactivator YAP. Tenascin-C also downregulates YAP pro-angiogenic target genes, thus reducing endothelial cell survival, proliferation, and tubulogenesis...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27924938/the-secretome-of-periodontal-ligament-stem-cells-from-ms-patients-protects-against-eae
#2
Thangavelu Soundara Rajan, Sabrina Giacoppo, Francesca Diomede, Patrizia Ballerini, Michele Paolantonio, Marco Marchisio, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani
Manipulation of stem cells or stem cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament stem cells (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients and healthy donors block experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing anti-inflammatory and immunosuppressive effects in spinal cord and spleen, and reverse disease progression by restoring tissue integrity via remyelination in the spinal cord...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27916652/exploring-pericyte-and-cardiac-stem-cell-secretome-unveils-new-tactics-for-drug-discovery
#3
REVIEW
Georgina M Ellison-Hughes, Paolo Madeddu
Ischaemic diseases remain a major cause of morbidity and mortality despite continuous advancements in medical and interventional treatments. Moreover, available drugs reduce symptoms associated with tissue ischaemia, without providing a definitive repair. Cardiovascular regenerative medicine is an expanding field of research that aims to improve the treatment of ischaemic disorders through restorative methods, such as gene therapy, stem cell therapy, and tissue engineering. Stem cell transplantation has salutary effects through direct and indirect actions, the latter being attributable to growth factors and cytokines released by stem cells and influencing the endogenous mechanisms of repair...
December 1, 2016: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27914072/identifying-bacterial-immune-evasion-proteins-using-phage-display
#4
Cindy Fevre, Lisette Scheepmaker, Pieter-Jan Haas
Methods aimed at identification of immune evasion proteins are mainly rely on in silico prediction of sequence, structural homology to known evasion proteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high-throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technology. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909049/control-of-secreted-protein-gene-expression-and-the-mammalian-secretome-by-the-metabolic-regulator-pgc-1%C3%AE
#5
Neri Minsky, Robert G Roeder
Secreted proteins serve pivotal roles in the development of multicellular organisms, acting as structural matrix, extracellular enzymes and signal molecules. However, how the secretome is regulated remains incompletely understood. Here we demonstrate, unexpectedly, that peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), a critical transcriptional co-activator of metabolic gene expression, functions to down-regulate expression of diverse genes encoding secreted molecules and extracellular matrix (ECM) components to modulate the secretome...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27908912/a-deep-proteome-analysis-identifies-the-complete-secretome-as-the-functional-unit-of-human-cardiac-progenitor-cells
#6
Sudhish Sharma, Rachana Mishra, Grace E Bigham, Brody Wehman, Mohd M Khan, Huichun Xu, Progyaparamita Saha, Young Ah Goo, Srinivisa R Datla, Ling Chen, Mohan E Tulapurkar, Bradley S Taylor, Peixin Yang, Sotirios K Karathanasis, David R Goodlett, Sunjay Kaushal
RATIONALE: Cardiac progenitor cells are an attractive cell type for tissue regeneration but their mechanism for myocardial remodeling is still unclear. OBJECTIVE: This investigation determines how chronological age influences the phenotypic characteristics and the secretome of human cardiac progenitor cells (CPCs), as well as their potential to recover injured myocardium. METHODS AND RESULTS: Adult (aCPCs) and neonatal (nCPCs) cells were derived from patients more than 40 years or less than one month of age, respectively, and their functional potential was determined in a rodent myocardial infarction (MI) model...
December 1, 2016: Circulation Research
https://www.readbyqxmd.com/read/27907906/senescent-fibroblast-derived-chemerin-promotes-squamous-cell-carcinoma-migration
#7
Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A Schneider, Medhanie A Mulaw, Tanja Lucas, Stefan Kochanek, Reinhard Dummer, Mitchell P Levesque, Meinhard Wlaschek, Karin Scharffetter-Kochanek
Aging is associated with a rising incidence of cutaneous squamous cell carcinoma (cSCC), an aggressive skin cancer with the potential for local invasion and metastasis. Acquisition of a senescence-associated secretory phenotype (SASP) in dermal fibroblasts has been postulated to promote skin cancer progression in elderly individuals. The underlying molecular mechanisms are largely unexplored. We show that Chemerin, a previously unreported SASP factor released from senescent human dermal fibroblasts, promotes cSCC cell migration, a key feature driving tumor progression...
November 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27907184/correction-mesenchymal-stromal-cell-secretome-up-regulates-47-kda-cxcr4-expression-and-induce-invasiveness-in-neuroblastoma-cell-lines
#8
Vipin Shankar, Hiroki Hori, Kentaro Kihira, Lei Qi, Hidemi Toyoda, Shotaro Iwamoto, Yoshihiro Komada
[This corrects the article DOI: 10.1371/journal.pone.0120069.].
2016: PloS One
https://www.readbyqxmd.com/read/27907131/itraq-based-quantitative-proteomic-comparison-of-early-and-late-passage-human-dermal-papilla-cell-secretome-in-relation-to-inducing-hair-follicle-regeneration
#9
Huan Zhang, Ning-Xia Zhu, Keng Huang, Bo-Zhi Cai, Yang Zeng, Yan-Ming Xu, Yang Liu, Yan-Ping Yuan, Chang-Min Lin
Alopecia is an exceedingly prevalent problem that lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential, we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs, and subcutaneously injected the DPC-CM into NU/NU mice...
2016: PloS One
https://www.readbyqxmd.com/read/27894935/brief-report-stage-specific-differences-in-secretory-profile-of-mesenchymal-stromal-cells-mscs-subjected-to-early-vs-late-stage-oa-synovial-fluid
#10
Alejandro Gómez-Aristizábal, Anirudh Sharma, Mohsen A Bakooshli, Mohit Kapoor, Penney M Gilbert, Sowmya Viswanathan, Rajiv Gandhi
OBJECTIVE: Although, mesenchymal stromal cells (MSCs) are being clinically investigated for their use in osteoarthritis (OA), it is unclear whether their postulated therapeutic properties are equally effective in the early- and late-stages of OA. In this study we investigated MSC cytokine secretion post-exposure to synovial fluid (SF), obtained from early-vs. late-stage knee OA patients to justify a potential patient stratification strategy to maximize MSC-mediated treatment effects. METHOD: Subjects were recruited and categorized into early- [Kellgren-Lawrence (KL) grade I/II, n = 12] and late-stage (KL-III/IV, n = 12) knee OA groups...
November 25, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27891773/cigarette-smoke-alters-the-secretome-of-lung-epithelial-cells
#11
Alessandra Mossina, Christina Lukas, Juliane Merl-Pham, Franziska E Uhl, Kathrin Mutze, Andrea Schamberger, Claudia Staab-Weijnitz, Jie Jia, Ali Ö Yildirim, Melanie Königshoff, Stefanie M Hauck, Oliver Eickelberg, Silke Meiners
Cigarette smoke is the most relevant risk factor for the development of lung cancer and chronic obstructive pulmonary disease (COPD). Many of its more than 4500 chemicals are highly reactive thereby altering protein structure and function. Here, we used subcellular fractionation coupled to label-free quantitative mass spectrometry to globally assess alterations in the proteome of different compartments of lung epithelial cells upon exposure to cigarette smoke extract. Proteomic profiling of the human alveolar-derived cell line A549 revealed the most pronounced changes within the cellular secretome with preferential downregulation of proteins involved in wound healing and extracellular matrix (ECM) organization...
November 28, 2016: Proteomics
https://www.readbyqxmd.com/read/27890076/dirofilaria-immitis-exhibits-sex-and-stage-specific-differences-in-excretory-secretory-mirna-and-protein-profiles
#12
Lucienne Tritten, Damian Clarke, Scott Timmins, Tom McTier, Timothy G Geary
The canine heartworm Dirofilaria immitis releases excretory/secretory molecules into its host and in culture. We report analyses of the types, amounts and stage-dependence of microRNAs and proteins found in D. immitis culture media recovered after incubating 800,000 microfilariae for 6days, 500L3 and 500L4 for 7days, as well as 40 adult females and 40 adult males for 48h, all separately. In addition, the presence of exosome-like particles was established by nanoparticle tracking analysis. Our results are in concordance with the D...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27888544/conditioned-medium-a-new-alternative-for-cryopreservation-of-equine-umbilical-cord-mesenchymal-stem-cells
#13
Leandro Maia, Marianne Camargos Dias, Carolina Nogueira de Moraes, Camila de Paula Freitas-Dell'aqua, Ligia S L Silveira da Mota, Valquíria Santiloni, Fernanda da Cruz Landim-Alvarenga
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies...
November 26, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27881474/cancer-secretome-may-influence-bsp-and-dsp-expression-in-human-salivary-gland-cells
#14
Samantha Lynn Hamilton, Blake Ferando, Asha Sarah Eapen, Jennifer Chian Yu, Anita Rose Joy
One of the biggest challenges in managing head and neck cancers, especially salivary gland cancers, is the identification of secreted biomarkers of the disease that can be evaluated noninvasively. A relevant source of enriched tumor markers could potentially be found in the tumor secretome. Although numerous studies have evaluated secretomes from various cancers, the influence of the cancer secretome derived from salivary gland cancers on the behavior of normal cells has not yet been elucidated. Our data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells...
November 23, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27872998/ppest-is-a-novel-pbat-degrading-polyesterase-identified-by-proteomic-screening-of-pseudomonas-pseudoalcaligenes
#15
Paal W Wallace, Karolina Haernvall, Doris Ribitsch, Sabine Zitzenbacher, Matthias Schittmayer, Georg Steinkellner, Karl Gruber, Georg M Guebitz, Ruth Birner-Gruenberger
A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome. PpEst was induced by the presence of PBAT in the growth media and had predicted arylesterase (EC 3.1.1.2) activity. PpEst showed polyesterase activity on both whole and milled PBAT film releasing terephthalic acid and 4-(4-hydroxybutoxycarbonyl)benzoic acid while end product inhibition by 4-(4-hydroxybutoxycarbonyl)benzoic acid was observed...
November 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27870168/proteome-and-secretome-characterisation-of-glioblastoma-derived-neural-stem-cells
#16
Satoshi Okawa, Sladjana Gagrica, Carla Blin, Christine Ender, Steven M Pollard, Jeroen Krijgsveld
Glioblastoma multiforme (GBM) (grade IV astrocytoma) is the most common and aggressive primary brain tumour. GBM consists of heterogeneous cell types includinga subset of stem cell-like cells thought to sustain tumour growth. These tumour-initiating GBM-derived neural stem (GNS) cells as well as their genetically normal neural stem (NS) counterparts can be propagated in culture as relatively pure populations. Here we perform quantitative proteomics to globally characterize and compare total proteome plus the secreted proteome (secretome) between GNS cells and NS cells...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27869176/quantitative-secretomic-analysis-of-pancreatic-cancer-cells-in-serum-containing-conditioned-medium
#17
Peng Liu, Yejing Weng, Zhigang Sui, Yunhao Wu, Xiangli Meng, Mengwei Wu, Haoyi Jin, Xiaodong Tan, Lihua Zhang, Yukui Zhang
Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies generally focus on the secretome of cancer cell line from serum-free media, due to the serious interference of fetal bovine serum (FBS). However, serum-starvation may alter expression patterns of secreted proteins. Hence, efforts to decrease the interference of serum in proteomic analysis of serum-containing media have been hampered to quantitatively measure the tumor secretion levels...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27866629/fungal-surface-protein-mediated-one-pot-synthesis-of-stable-and-hemocompatible-gold-nanoparticles
#18
Michael Kitching, Priyadarshani Choudhary, Saikumar Inguva, Yina Guo, Meghana Ramani, Sujoy K Das, Enrico Marsili
Despite their large secretome and wide applications in bioprocesses, fungi remain underexplored in metal nanoparticles (MNP) biosynthesis. Previous studies have shown that cell surface proteins of Rhizopus oryzae play a crucial role in biomineralization of Au(III) to produce gold nanoparticles (AuNPs). Therefore, it is hypothesized that purified cell surface protein may produce in vitro AuNPs with narrow size distribution for biomedical and biocatalytic applications. However, different protein extraction methods might affect protein stability and the AuNP biosynthesis process...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27865962/pharmacologically-active-microcarriers-delivering-bdnf-within-a-hydrogel-novel-strategy-for-human-bone-marrow-derived-stem-cells-neural-neuronal-differentiation-guidance-and-therapeutic-secretome-enhancement
#19
Saikrishna Kandalam, Laurence Sindji, Gaëtan J-R Delcroix, Fabien Violet, Xavier Garric, Emilie M André, Paul C Schiller, Marie-Claire Venier-Julienne, Anne des Rieux, Jérôme Guicheux, Claudia N Montero-Menei
: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly in the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem cells and pharmacologically active microcarriers (PAMs) into an injectable non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain an injectable non-toxic cell and growth factor delivery device...
November 16, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27865792/a-quantitative-proteomic-screen-of-the-campylobacter-jejuni-flagellar-dependent-secretome
#20
Eoin Scanlan, Lu Yu, Duncan Maskell, Jyoti Choudhary, Andrew Grant
: Campylobacter jejuni is the leading cause of bacterial gastroenteritis in the world. A number of factors are believed to contribute to the ability of C. jejuni to cause disease within the human host including the secretion of non-flagellar proteins via the flagellar type III secretion system (FT3SS). Here for the first time we have utilised quantitative proteomics using stable isotope labelling by amino acids in cell culture (SILAC), and label-free liquid chromatography-mass spectrometry (LC/MS), to compare supernatant samples from C...
November 16, 2016: Journal of Proteomics
keyword
keyword
61013
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"