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https://www.readbyqxmd.com/read/28632775/unique-cellular-interactions-between-pancreatic-cancer-cells-and-the-omentum
#1
Valerya Feygenzon, Shelly Loewenstein, Nir Lubezky, Metsada Pasmanic-Chor, Osnat Sher, Joseph M Klausner, Guy Lahat
Pancreatic cancer is a common cause of cancer-related mortality. Omental spread is frequent and usually represents an ominous event, leading to patient death. Omental metastasis has been studied in ovarian cancer, but data on its role in pancreatic cancer are relatively scarce and the molecular biology of this process has yet to be explored. We prepared tissue explants from human omental fat, and used conditioned medium from the explants for various in vitro and in vivo experiments designed to evaluate pancreatic cancer development, growth, and survival...
2017: PloS One
https://www.readbyqxmd.com/read/28627741/proteomics-approach-to-identify-novel-metastatic-bone-markers-from-the-secretome-of-pc-3-prostate-cancer-cells
#2
EunJi Sung, Oh Kwang Kwon, Jae-Mok Lee, Sangkyu Lee
Prostate cancer is the leading type of cancer diagnosed, and the most frequent cause of worldwide male cancer-related deaths annually. The limitations of current prostate cancer screening tests demand the identification of novel biomarkers for the early diagnosis of prostate cancer bone metastasis. In the present study, we performed a proteomic analysis of secreted proteins from the prostate cancer bone metastasis cell line, PC-3, and the normal prostate cell line, RWPE-1. We thus quantified 917 proteins, of which 68 were found to be secreted at higher levels by PC-3 than by RWPE-1 cells via LC-MS/MS...
June 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28621210/effects-of-lucilia-sericata-on-wound-healing-in-streptozotocin-induced-diabetic-rats-and-analysis-of-its-secretome-at-the-proteome-level
#3
F K Tombulturk, M Kasap, M Tuncdemir, E Polat, S Sirekbasan, A Kanli, G Kanigur-Sultuybek
The use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28619171/proteomic-and-bioinformatic-analyses-of-putative-mannheimia-haemolytica-secretome-by-liquid-chromatography-and-tandem-mass-spectrometry
#4
Sahlu Ayalew, Anthony W Confer, Steve D Hartson, Patricia J Canaan, Mark Payton, Brian Couger
Mannheimia haemolytica is a major bacterial contributor to bovine respiratory disease complex that costs the livestock industry a billion dollars a year in USA. Commercial vaccines are only partially efficacious under field conditions. Earlier studies found that outer membrane protein preparations and culture supernatants can induce immune responses that enhance resistance to challenge by M. haemolytica strains. The objective of this study was to characterize secretome of two M. haemolytica stains grown under two different media...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28610997/identification-of-multienzymatic-complexes-in-the-clonostachys-byssicola-secretomes-produced-in-response-to-different-lignocellulosic-carbon-sources
#5
Helder Andrey Rocha Gomes, Adelson Joel da Silva, Diana Paola Gómez-Mendoza, Agenor Castro Moreira Dos Santos Júnior, Nicholas de Mojana di Cologna, Rosane Mansan Almeida, Robert Neil Gerard Miller, Wagner Fontes, Marcelo Valle de Sousa, Carlos André Ornelas Ricart, Edivaldo Ximenes Ferreira Filho
Multienzymatic complexes with plant lignocellulose-degrading activities have recently been identified in filamentous fungi secretomes. Such complexes have potential biotechnological applications in the degradation of agro-industrial residues. Fungal species from the Clonostachys genus have been intensively investigated as biocontrol agents; however so far their use as producers of lignocellulose-degrading enzymes has not been extensively explored. Secretomes of Clonostachys byssicola following growth on different carbon sources (passion fruit peel, soybean hulls, cotton gin trash, banana stalk, sugarcane bagasse, orange peel, and a composition of soybean hulls: cotton gin trash:orange peel) were subjected to enzymatic assays...
June 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28608858/telocytes-facts-speculations-and-myths-review-article
#6
Veronika Aleksandrovych, Artur Pasternak, Paweł Basta, Marek Sajewicz, Jerzy A Walocha, Krzysztof Gil
Telocyte (TC) is an interstitial cell type with a small cellular body and extremely long tentacle-like extensions. TCs were discovered a decade ago and have specifc morphological characteristics, immunohistochemical and secretome profiles, electrophysiological properties, microRNA expression. Moreover, they are different in gene expression from other cells. TCs play an important role in plenty of processes. Apparently, they are involved in homeostasis, remodelling, regeneration, repair, embryogenesis, angiogenesis and even tumorigenesis...
2017: Folia Medica Cracoviensia
https://www.readbyqxmd.com/read/28605657/muscle-as-a-paracrine-and-endocrine-organ
#7
REVIEW
Jimena Giudice, Joan M Taylor
Skeletal muscle cells are highly abundant and metabolically active and are known to 'communicate' their energy demands to other organs through active secretion. Muscle-derived secretory proteins include a variety of cytokines and peptides collectively referred to as 'myokines' that exert autocrine, paracrine or endocrine effects. Analyses of the skeletal muscle secretome revealed that numerous myokines are secreted in response to contraction or strength training, and that these factors not only regulate energy demand but also contribute to the broad beneficial effects of exercise on cardiovascular, metabolic, and mental health...
June 9, 2017: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/28602759/construction-and-screening-of-a-lentiviral-secretome-library
#8
Tao Liu, Panpan Jia, Huailei Ma, Sean A Reed, Xiaozhou Luo, H Benjamin Larman, Peter G Schultz
Over 2,000 human proteins are predicted to be secreted, but the biological function of the many of these proteins is still unknown. Moreover, a number of these proteins may act as new therapeutic agents or be targets for the development of therapeutic antibodies. To further explore the extracellular proteome, we have developed a secretome-enriched open reading frame (ORF) library that can be readily screened for autocrine activity in cell-based phenotypic or reporter assays. Next-generation sequencing (NGS) and database analysis predict that the library contains approximately 900 ORFs encoding known secreted proteins (accounting for 77...
May 31, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28600981/pharmacologic-concentrations-of-linezolid-modify-oxidative-phosphorylation-function-and-adipocyte-secretome
#9
Laura Llobet, M Pilar Bayona-Bafaluy, David Pacheu-Grau, Elena Torres-Pérez, José M Arbones-Mainar, M Ángeles Navarro, Covadonga Gómez-Díaz, Julio Montoya, Ester López-Gallardo, Eduardo Ruiz-Pesini
The oxidative phosphorylation system is important for adipocyte differentiation. Therefore, xenobiotics inhibitors of the oxidative phosphorylation system could affect adipocyte differentiation and adipokine secretion. As adipokines impact the overall health status, these xenobiotics may have wide effects on human health. Some of these xenobiotics are widely used therapeutic drugs, such as ribosomal antibiotics. Because of its similarity to the bacterial one, mitochondrial translation system is an off-target for these compounds...
May 31, 2017: Redox Biology
https://www.readbyqxmd.com/read/28599499/comparative-secretome-of-ovarian-serous-carcinoma-gelsolin-in-the-spotlight
#10
Sandra Pierredon, Pascale Ribaux, Jean-Christophe Tille, Patrick Petignat, Marie Cohen
Ovarian cancer is one of the most common types of reproductive cancer, and has the highest mortality rate amongst gynecological cancer subtypes. The majority of ovarian cancers are diagnosed at an advanced stage, resulting in a five-year survival rate of ~30%. Early diagnosis of ovarian cancer has improved the five-year survival rate to ≥90%, thus the current imperative requirement is to identify biomarkers that would allow the early detection, diagnosis and monitoring of the progression of the disease, or of novel targets for therapy...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28586545/multifaceted-therapeutic-benefits-of-factors-derived-from-stem-cells-from-human-exfoliated-deciduous-teeth-for-acute-liver-failure-in-rats
#11
Yoshihiro Matsushita, Masatoshi Ishigami, Kohki Matsubara, Megumi Kondo, Hirotaka Wakayama, Hidemi Goto, Minoru Ueda, Akihito Yamamoto
In acute liver failure (ALF), a poorly controlled innate immune response causes massive hepatic destruction, which elicits a systemic inflammatory response, progressive multiple organ failure and ultimate sudden death. Although the liver has inherent tissue-repairing activities, its regeneration during ALF fails, and orthotopic liver transplantation is the only curative approach. Here we show that a single intravenous administration of stem cells derived from human exfoliated deciduous teeth (SHEDs) or of SHED-derived serum-free conditioned medium (SHED-CM) into the d-galactosamine-induced rat model of ALF markedly improved the condition of the injured liver and the animals' survival rate...
June 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28585531/small-extracellular-vesicles-secreted-from-senescent-cells-promote-cancer-cell-proliferation-through-epha2
#12
Masaki Takasugi, Ryo Okada, Akiko Takahashi, David Virya Chen, Sugiko Watanabe, Eiji Hara
Cellular senescence prevents the proliferation of cells at risk for neoplastic transformation. However, the altered secretome of senescent cells can promote the growth of the surrounding cancer cells. Although extracellular vesicles (EVs) have emerged as new players in intercellular communication, their role in the function of senescent cell secretome has been largely unexplored. Here, we show that exosome-like small EVs (sEVs) are important mediators of the pro-tumorigenic function of senescent cells. sEV-associated EphA2 secreted from senescent cells binds to ephrin-A1, that is, highly expressed in several types of cancer cells and promotes cell proliferation through EphA2/ephrin-A1 reverse signalling...
June 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28582578/proteomics-of-the-epicardial-fat-secretome-and-its-role-in-post-operative-atrial-fibrillation
#13
Alessandro Viviano, Xiaoke Yin, Anna Zampetaki, Marika Fava, Mark Gallagher, Manuel Mayr, Marjan Jahangiri
Aims: Post-operative atrial fibrillation (POAF) is a predictor of morbidity and mortality after cardiac surgery. Latent predisposing factors may reside in the epicardial adipose tissue (EAT) due to its anatomical position and high protein production rate. In order to explore a possible mechanistic link, we characterized proteins secreted by the EAT preceding the onset of POAF. Methods and results: Epicardial adipose tissue samples were collected from 76 consecutive patients with no history of AF undergoing coronary artery bypass surgery, 50 samples for proteomic analysis and 26 for gene expression studies, further divided according to development of POAF...
June 5, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28582523/the-interplay-between-adipose-tissue-and-the-cardiovascular-system-is-fat-always-bad
#14
Ioannis Akoumianakis, Charalambos Antoniades
Obesity is a risk factor for cardiovascular disease (CVD). However, clinical research has revealed a paradoxically protective role for obesity in patients with chronic diseases including CVD, suggesting that the biological "quality" of adipose tissue (AT) may be more important than overall AT mass or body weight. Importantly, AT is recognized as a dynamic organ secreting a wide range of biologically active adipokines, microRNAs, gaseous messengers and other metabolites that affect the cardiovascular system in both endocrine and paracrine ways...
June 5, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28567337/body-mass-index-is-associated-with-region-dependent-metabolic-reprogramming-of-adipose-tissue
#15
Marco G Alves, Ângela Moreira, Marta Guimarães, Mário Nora, Mario Sousa, Pedro F Oliveira, Mariana P Monteiro
Adipose tissue (AT) is involved in dysmetabolism pathogenesis. Regional fat distribution and functioning may contribute to obesity-related metabolic disorders and adverse health outcomes. Specific fat depots are suggested to possess unique biological properties, but specific metabolic profiles of subcutaneous (SAT) and visceral adipose tissue (VAT) remain unknown. We aimed to characterize VAT and SAT glucose metabolism, and their correlation with body mass index (BMI). AT samples from patients (n = 12; F:M, 9:3) with a mean age of 46 years (26-83 years) and an average BMI of 29...
December 2017: BBA Clinical
https://www.readbyqxmd.com/read/28560424/secretomic-profiling-of-cells-from-hollow-fiber-bioreactor-reveals-psma3-as-a-potential-cholangiocarcinoma-biomarker
#16
Chris Verathamjamras, Churat Weeraphan, Daranee Chokchaichamnankit, Kamolwan Watcharatanyatip, Pantipa Subhasitanont, Penchatr Diskul-Na-Ayudthaya, Kanokwan Mingkwan, Virat Luevisadpaibul, Somchai Chutipongtanate, Voraratt Champattanachai, Jisnuson Svasti, Chantragan Srisomsap
Cholangiocarcinoma (CCA), derived from the bile duct, occurs with a relatively high incidence in Northeast Thailand. Early diagnosis is still hampered by the lack of sufficient biomarkers. In recent years, biomarker discovery using secretomes has provided interesting results, including our studies on CCA secretomes, especially with three-dimensional cell cultures. Thus, cells cultured using the hollow fiber bioreactor (HFB) with 20 kDa molecular weight cut-off (MWCO) yielded higher quality and quantity of secretomes than those from conditioned media of the monolayer culture (MNC) system...
May 29, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28557053/a-novel-streptococcal-cell-cell-communication-peptide-promotes-pneumococcal-virulence-and-biofilm-formation
#17
Rolando A Cuevas, Rory Eutsey, Anagha Kadam, Jacob A West-Roberts, Carol A Woolford, Aaron P Mitchell, Kevin M Mason, N Luisa Hiller
Streptococcus pneumoniae (pneumococcus) is a major human pathogen. It is a common colonizer of the human respiratory track, where it utilizes cell-cell communication systems to coordinate population-level behaviors. We reasoned that secreted peptides that are highly expressed during infection are pivotal for virulence. Thus, we used in silico pattern searches to define a pneumococcal secretome, and analyzed the transcriptome of the clinically important PMEN1 lineage to identify which peptide-encoding genes are highly expressed in vivo...
May 29, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28554583/autologous-and-allogeneic-cardiac-stem-cell-therapy-for-cardiovascular-diseases
#18
REVIEW
Ricardo Sanz-Ruiz, Francisco Fernández-Avilés
Stem cell therapy is one of the most promising therapeutic innovations to help restore cardiac structure and function after ischemic insults to the heart. However, phase I and II clinical trials with autologous "first-generation stem cells" have yielded inconsistent results in ischemic cardiomyopathy patients and have not produced the definitive evidence for their broad clinical application. Recently, new cell types such as cardiac stem cells (CSC) and new allogeneic sources have attracted the attention of researchers given their inherent biological, clinical and logistic advantages...
May 26, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28549096/the-molecular-mechanisms-of-obesity-paradox
#19
Alexios S Antonopoulos, Dimitris Tousoulis
Clinical observations suggest a complex relationship between human obesity and cardiovascular disease. Whilst abdominal (visceral) adiposity leads to deleterious metabolic disturbances, subcutaneous fat accumulation has a benign effect on cardiometabolic risk. Notably, an accumulating body of evidence paradoxically links increased body mass index with a better prognosis in patients with established cardiovascular disease, a finding that has been termed the 'obesity paradox'. While this is now acknowledged to be an epidemiological finding, a metabolically healthy obese group associated with low cardiovascular risk has also been identified...
May 26, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28547891/large-scale-gene-expression-reveals-different-adaptations-of-hyalopterus-persikonus-to-winter-and-summer-host-plants
#20
Na Cui, Peng-Cheng Yang, Kun Guo, Le Kang, Feng Cui
Host alternation, an obligatory seasonal shifting between host plants of distant genetic relationship, has had significant consequences for the diversification and success of the superfamily of aphids. However, the underlying molecular mechanism remains unclear. In this study, the molecular mechanism of host alternation was explored through a large-scale gene expression analysis of the mealy aphid Hyalopterus persikonus on winter and summer host plants. More than four times as many unigenes of the mealy aphid were significantly upregulated on summer host Phragmites australis than on winter host Rosaceae plants...
June 2017: Insect Science
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