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Nicholas L Adkins, Sarah G Swygert, Parminder Kaur, Hengyao Niu, Sergei A Grigoryev, Patrick Sung, Hong Wang, Craig L Peterson
Repair of DNA double strand breaks (DSBs) is key for maintenance of genome integrity. When DSBs are repaired by homologous recombination, DNA ends can undergo extensive processing, producing long stretches of single-stranded DNA (ssDNA). In vivo, DSB processing occurs in the context of chromatin, and studies indicate that histones may remain associated with processed DSBs. Here we demonstrate that histones are not evicted from ssDNA after in vitro chromatin resection. In addition, we reconstitute histone-ssDNA complexes (termed ssNucs) with ssDNA and recombinant histones and analyze these particles by a combination of native gel electrophoresis, sedimentation velocity, electron microscopy, and a recently developed electrostatic force microscopy technique, DREEM (Dual-Resonance-frequency-Enhanced Electrostatic force Microscopy)...
February 15, 2017: Journal of Biological Chemistry
Rehana Rehman, Kulsoom Ghias, Syeda Sadia Fatima, Mehwish Hussain, Faiza Alam
OBJECTIVE: To compare students' response assessed by Dundee Ready Educational Environment Measure on the basis of the year of study, gender and pre-medical educational background. METHODS: This cross-sectional study was carried out at the Aga Khan University Medical College, Karachi, Pakistan, from June 2014 to March 2015, and comprised medical students. The average scores of Dundee Ready Educational Environment Measure scales and subscales were compared between gender and educational background...
January 2017: JPMA. the Journal of the Pakistan Medical Association
Brendan P Condon, Paul S Worley, John R Condon, David J Prideaux
OBJECTIVES: The Deakin University School of Medicine commenced in 2008 as a rurally focused medical school in south-eastern Australia. This research was designed to examine the effectiveness of the school's adoption of small regional clinical school settings. METHODS: A retrospective cohort study of the first two cohorts of students was employed to assess academic performance at each of five geographically dispersed clinical training sites, with varying student cohort sizes...
December 30, 2016: Medical Teacher
Gideon Victor, Muhammad Ishtiaq, Subia Parveen
PURPOSE: The objective of this study was to evaluate nursing students' perceptions of their educational environment in a private college. Perceptions were compared between genders and 2 bachelor's programs. METHODS: A total of 219 students participated in this study, drawn from the Generic Bachelor of Science in Nursing (GBSN) and the Post-Registered Nurse Bachelor of Science in Nursing (PRBSN) programs of the Shifa College of Nursing, Islamabad, Pakistan. The Dundee Ready Education Environment Measure was utilized for data collection...
2017: Journal of Educational Evaluation for Health Professions
Hyunho Kim, Hanyoung Jeong, Pyeongjin Jeon, Seungju Kim, Young-Bae Park, Yeonseok Kang
Background. In Korea, a few studies regarding traditional Korean medicine (TKM) education have been conducted. The aim of this study is to evaluate students' perceptions regarding TKM education in Korea and compare them with those of other countries using a quantitative scale, Dundee Ready Educational Environment Measure (DREEM). Materials and Methods. We conducted a survey using DREEM in a TKM college. Totally, 325 students responded to this survey and we performed the descriptive statistics of scores in all items, subscales, and total...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Efrain Riveros-Perez, Ricardo Riveros, Nicole M Zimmerman, Alparsian Turan
STUDY OBJECTIVES: To assess whether the year of residency in anesthesiology is associated with residents' overall perceptions of the learning environment and to evaluate the association between year of residency and each of the five domains of the Dundee Ready Education Environment Measure (DREEM) survey. DESIGN: Cross-sectional study. SETTING: Residency program of anesthesiology at the Cleveland Clinic. SUBJECTS AND INTERVENTION: Residents of anesthesiology at the Cleveland Clinic were invited to complete the DREEM questionnaire and a demographic survey...
December 2016: Journal of Clinical Anesthesia
Michail Ch Sideris, Apostolos E Papalois, Thanos Athanasiou, Ioannis Dimitropoulos, Korina Theodoraki, Francois Sousa Dos Santos, Georgios Paparoidamis, Nikolaos Staikoglou, Dimitrios Pissas, Peter C Whitfield, Alexandros Rampotas, Savvas Papagrigoriadis, Vassilios Papalois, Georgios Zografos, Georgios Tsoulfas
BACKGROUND: Essential Skills in the management of Surgical Cases - ESMSC is an International Combined Applied Surgical Science and Wet Lab course aimed at the undergraduate level. ESMSC combines interactive basic science workshops and case-based learning, with basic surgical training modules (BST) on Ex Vivo and In Vivo swine model. In Vivo Dissections include more advanced modules i.e. Abdominal Anatomy Dissections and Cardiac Transplant. AIM: To evaluate the educational environment of a novel course, as well as to compare Medical students' perceptions across various groups...
December 2016: Annals of Medicine and Surgery
Jonathan M Barcelo
PURPOSE: This study aimed to compare the perception of the academic learning environment between medical laboratory science students and nursing students at Saint Louis University, Baguio City, Philippines. METHODS: A cross-sectional survey research design was used to measure the perceptions of the participants. A total of 341 students from the Department of Medical Laboratory Science, School of Natural Sciences, and the School of Nursing answered the Dundee Ready Education Environment Measure (DREEM) instrument from April to May 2016...
2016: Journal of Educational Evaluation for Health Professions
Kyaimon Myint, Hoe See-Ziau, Ruby Husain, Rosnah Ismail
BACKGROUND: An equitable and positive learning environment fosters deep self-directed learning in students and, consequently, good practice in their profession. Although demotivating weaknesses may lead to repeated day-to-day stress with a cascade of deleterious consequences at both personal and professional levels, a possible relationship between these parameters has not been reported. This study was undertaken to determine the relationship between students' perceptions of their educational environment and their stress levels...
May 2016: Malaysian Journal of Medical Sciences: MJMS
Rehana Rehman, Kulsoom Ghias, Syeda Sadia Fatima, Mehwish Hussain, Faiza Alam
OBJECTIVE: To assess educational environment in Aga Khan University Medical College (AKUMC) by Dundee Ready Educational Environmental Measure (DREEM) scale. METHODS: A cross-sectional survey of students at the AKUMC with simple random sampling was carried out from June 2014 till March 2015. Responses in five subscales were used to calculate DREEM scores. RESULTS: The average DREEM score was 125.77±16.8 with a reliability of 91.3%. With regards to subscales, on the 12-item students' perceptions of learning (PoL) subscale, the maximum score was 48; 11 items of students' perceptions of teachers (PoT) had a maximum score of 44; students' academic self-perceptions (ASP) identified by 8 items showed maximum score of 32; students' perceptions of atmosphere (PoA) with 12 items obtained maximum score 48 and students' social self-perceptions (SSP) subscale of 7 items had a maximum score of 28...
May 2016: Pakistan Journal of Medical Sciences Quarterly
Asmita Ashok Patil, Vijaya Laxman Chaudhari
PURPOSE: The educational environment (EE) plays a very important role in effective student learning. The Dundee Ready Education Environment Measure (DREEM) is a validated tool to assess the EE. This study aimed to collect baseline information about our medical student's perception of the EE, and to identify areas of strengths and weaknesses as well as scope for improvements in the current EE. METHODS: Medical students and interns were included in this cross-sectional study...
September 2016: Korean Journal of Medical Education
Xin Xu, Daxing Wu, Xiaohua Zhao, Junxiang Chen, Jie Xia, Mulei Li, Xueqing Nie, Xue Zhong
BACKGROUND: Perceived educational environment influences academic outcomes, such as academic achievement, students' behaviors, well-being, socio-emotional adjustment and explicit self-esteem. Mindfulness is a set of skills that are beneficial to physical and mental health. Recently, it has been increasingly discussed about its usefulness in education, but little research has explored whether mindfulness can predict perceptions of educational environment. The aim of this study was to explore Chinese medical students' perceptions of learning environment and their relationship with mindfulness...
2016: Medical Education Online
Panteleimon Pantelidis, Nikolaos Staikoglou, Georgios Paparoidamis, Christos Drosos, Stefanos Karamaroudis, Athina Samara, Christodoulos Keskinis, Michail Sideris, George Giannakoulas, Georgios Tsoulfas, Asterios Karagiannis
PURPOSE: The integration of simulation-based learning (SBL) methods holds promise for improving the medical education system in Greece. The Applied Basic Clinical Seminar with Scenarios for Students (ABCS3) is a novel two-day SBL course that was designed by the Scientific Society of Hellenic Medical Students. The ABCS3 targeted undergraduate medical students and consisted of three core components: the case-based lectures, the ABCDE hands-on station, and the simulation-based clinical scenarios...
2016: Journal of Educational Evaluation for Health Professions
Victor Mogre, Anthony Amalba
BACKGROUND: The Dundee Ready Educational Environment Measure (DREEM) has been widely accepted and recognized for the assessment of the educational environment in a variety of health professions education programs. Concerns regarding the psychometric properties of the DREEM have been raised. This study evaluated the psychometric properties of the DREEM in a sample of Ghanaian medical students following a problem-based learning curriculum. METHODS: A sample of 234 second to fourth year medical students of the University for Development Studies, School of Medicine and Health Sciences were invited to complete the DREEM questionnaire...
January 2016: Education for Health: Change in Training & Practice
Sushant Chhabra, Asit Misra, Sumiyah Shah, Tamorish Kole
BACKGROUND: The specialty of emergency medicine is in its infancy state in the long history of the Indian health sciences education system. Little analytical published data is available at the moment in India regarding the quality of medical education as perceived by the students. Roff et al. (Med Teach 19: 295-299, 1997) developed a methodology using a Delphi panel to standardize the measurement of medical education known as the Dundee Ready Education Environment Measure (DREEM), which is widely utilized...
December 2016: International Journal of Emergency Medicine
Parminder Kaur, Dong Wu, Jiangguo Lin, Preston Countryman, Kira C Bradford, Dorothy A Erie, Robert Riehn, Patricia L Opresko, Hong Wang
Shelterin protein TRF2 modulates telomere structures by promoting dsDNA compaction and T-loop formation. Advancement of our understanding of the mechanism underlying TRF2-mediated DNA compaction requires additional information regarding DNA paths in TRF2-DNA complexes. To uncover the location of DNA inside protein-DNA complexes, we recently developed the Dual-Resonance-frequency-Enhanced Electrostatic force Microscopy (DREEM) imaging technique. DREEM imaging shows that in contrast to chromatin with DNA wrapping around histones, large TRF2-DNA complexes (with volumes larger than TRF2 tetramers) compact DNA inside TRF2 with portions of folded DNA appearing at the edge of these complexes...
February 9, 2016: Scientific Reports
Luis Carlos Domínguez, Neil Valentín Vega, Erik Leonardo Espitia, Álvaro Enrique Sanabria, Claudia Corso, Adriana Margarita Serna, Camilo Osorio
INTRODUCTION: The Facultad de Medicina of the Universidad de La Sabana routinely uses lectures as the major educational strategy in clinical areas. Since 2012, a curriculum in context and a flipped classroom were introduced in the surgery course. OBJECTIVE: To compare the impact of lectures versus the flipped classroom model in the learning environment in surgery. MATERIALS AND METHODS: The Dundee Ready Education Environment Measure (DREEM) questionnaire was administered to four cohorts of students...
October 2015: Biomédica: Revista del Instituto Nacional de Salud
Dong Wu, Parminder Kaur, Zimeng M Li, Kira C Bradford, Hong Wang, Dorothy A Erie
Many cellular functions require the assembly of multiprotein-DNA complexes. A growing area of structural biology aims to characterize these dynamic structures by combining atomic-resolution crystal structures with lower-resolution data from techniques that provide distributions of species, such as small-angle X-ray scattering, electron microscopy, and atomic force microscopy (AFM). A significant limitation in these combinatorial methods is localization of the DNA within the multiprotein complex. Here, we combine AFM with an electrostatic force microscopy (EFM) method to develop an exquisitely sensitive dual-resonance-frequency-enhanced EFM (DREEM) capable of resolving DNA within protein-DNA complexes...
January 21, 2016: Molecular Cell
Uma Bhosale
INTRODUCTION: Educational Environment (EE) has significant impact on teaching-learning, satisfaction, performance and academic progress of students. Feedback obtained through structured questionnaire designed for them can serve as tool for identifying and solving these EE related problems. OBJECTIVE: To assess the perceptions of medical students concerning their educational environment (EE) using Dundee Ready Education Environment Measure (DREEM) scale. MATERIALS AND METHODS: Study involved all three years medical students; surveyed with DREEM questionnaire consisted of 50 items based on the Likert 's scale (scores from 0 to 200); and 5 domains namely students' perceptions of learning (SPL), perceptions of teachers (SPT), academic self-perceptions (SAP), perceptions of atmosphere (SPA) and social self-perceptions (SSP)...
November 2015: Journal of Clinical and Diagnostic Research: JCDR
Jihane Belayachi, Rachid Razine, Amina Boufars, Asma Saadi, Naoufal Madani, Souad Chaouir, Redouane Abouqal
This study aimed to assess students' perceptions of their educational environment in the Faculty of Medicine and Pharmacy of Rabat, Morocco, using the Dundee Ready Educational Environment Measure (DREEM). A cross-sectional survey was conducted in the Faculty of Medicine and Pharmacy of Rabat, Morocco, in which medical students' perceptions of their educational environment were assessed using the DREEM criteria during the 2013-2014 academic years. The DREEM inventory encompasses 50 items divided into five subdomains: perceptions of learning, perceptions of teaching, academic self-perceptions, perceptions of atmosphere, and social self-perceptions...
2015: Journal of Educational Evaluation for Health Professions
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