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https://www.readbyqxmd.com/read/29029217/high-levels-of-susceptibility-to-new-and-older-antibiotics-in-neisseria-gonorrhoeae-isolates-from-saskatchewan-2003-15-time-to-consider-point-of-care-or-molecular-testing-for-precision-treatment
#1
Sidharath D Thakur, Paul N Levett, Gregory B Horsman, Jo-Anne R Dillon
Objectives: The antimicrobial susceptibility of Neisseria gonorrhoeae isolates from Saskatchewan was determined retrospectively (2003-15) to ascertain temporal trends to both current and older antimicrobials used for treatment. Method: The agar dilution method was used to test the antimicrobial susceptibilities of 685 isolates to seven antibiotics. Results: Over the period, only three (0.4%) gonococcal isolates had reduced susceptibility to cefixime and/or ceftriaxone...
September 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29028615/determination-of-quinolones-in-wastewater-by-porous-%C3%AE-cyclodextrin-polymer-based-solid-phase-extraction-coupled-with-hplc
#2
Jingyi Zhang, Donghao Liu, Yi Shi, Chen Sun, Muchuan Niu, Ruya Wang, Fan Hu, Deli Xiao, Hua He
In this research, a novel insoluble sorbent based on cyclodextrin and rigid aromatic groups tetrafluoroacetonitrile was designed for dispersive insoluble solid-phase extraction (DSPE). Due to its high adsorption capacity, this obtained polymer was applied to separation and concentration of trace quinolones in wastewater before HPLC determination. Various parameters influencing the extraction performance were studied and optimized. A DSPE approach coupled with high performance liquid chromatography was developed for the determination of four quinolones in wastewater samples...
October 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29028568/virulence-genotypes-and-antimicrobial-susceptibility-patterns-of-arcobacter-butzleri-isolated-from-seafood-and-its-environment
#3
Srinu Rathlavath, Vandita Kohli, Asem Sanjit Singh, Manjusha Lekshmi, Gayatri Tripathi, Sanath Kumar, Binaya Bhusan Nayak
Arcobacter butzleri is an emerging pathogen isolated from animals, food and the environment. In this study, 147 A. butzleri isolated from seafood and the coastal environment were tested for the presence of ten putative virulence genes (cadF, cj1349, ciaB, mviN, pldA, tlyA, hecA, hecB, irgA, iroE) and antimicrobial susceptibilities. Majority of the isolates harbored mviN (100%), cj1349 (97.2%), ciaB (95.9%), tlyA (91.8%), pldA (91.1%) and cadF (89.7%). Lower detection rates were observed for hecA (10.8%), hecB (19%), iroE (12...
October 3, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29026795/the-prevalence-of-plasmid-mediated-quinolone-resistance-genes-in-escherichia-coli-isolated-from-hospital-wastewater-sources-in-tehran-iran
#4
Reza Ranjbar, Omid Farahani
BACKGROUND: Considering the importance of hospital wastewaters as potential reservoirs for the dissemination of bacterial pathogens such as Escherichia coli and antibiotic resistance genes in the environment, the need for such information becomes imperative. METHODS: E. coli strains were isolated from hospital wastewater sources in Tehran, Iran, over a 24-month sampling period (Jun 2014- Jun 2016) and identified using standard bacteriological methods. Quinolone resistance among the strains was determined using Kirby-Bauer method and the frequency of plasmid-mediated quinolone resistance genes (qnrA, qnrB, qnrS) was investigated by PCR...
September 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29026630/sinister-seafood-bacteraemia-secondary-to-non-o1-o139-vibrio-cholerae-infection
#5
Maxime J Billick, Philip W Lam, Isaac I Bogoch
Introduction.Vibrio species are curved, motile Gram-negative bacilli found in estuarine and marine environments, and are known to cause to gastroenteritis, skin and soft tissue infections, and septicaemia. While not responsible for cholera epidemics, non-O1/O139 Vibrio cholerae (NOVC) is increasingly reported as a cause of gastroenteritis. Case presentation. A 66-year-old man presented to an emergency department with a 1 week history of epigastric pain, emesis and fever. Blood cultures drawn on admission initially demonstrated Gram-negative bacilli, and ultimately grew NOVC, which was later confirmed by matrix-assisted laser desorption ionization-time of flight MS...
July 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29023394/carbon-based-oxamate-cobalt-iii-complexes-as-bioenzyme-mimics-for-contaminant-elimination-in-high-backgrounds-of-complicated-constituents
#6
Nan Li, Yun Zheng, Xuemei Jiang, Ran Zhang, Kemei Pei, Wenxing Chen
Complex wastewater with massive components is now a serious environmental issue facing humanity. Selective removal of low-concentration contaminants in mixed constituents holds great promise for increasing water supplies. Bioenzymes like horseradish peroxidase exhibit oxidizing power and selectivity. Here, we manufactured its mimic through immobilizing non-heme oxamate anionic cobalt(III) complex ([Co(III)(opba)](-), opba = o-phenylenebis(oxamate)) onto pyridine (Py) modified multiwalled carbon nanotubes ([Co(III)(opba)](-)-Py-MWCNTs, MWCNTs = multiwalled carbon nanotubes), where MWCNTs captured substrates and Py functioned as the fifth ligand...
October 12, 2017: Materials
https://www.readbyqxmd.com/read/29023074/bacterial-quality-of-urinary-tract-infections-in-diabetic-and-non-diabetics-of-the-population-of-ma-an-province-jordan
#7
Ali Al-Asoufi, Ali Khlaifat, Amjad Al Tarawneh, Khalid Alsharafa, Muhamad Al-Limoun, Khaled Khleifat
BACKGROUND AND OBJECTIVE: The patients with Diabetes Mellitus (DM) have malfunction in bladder which prompt urine accumulation in its pool which serves a decent situation to the microbes to be develop and cause Urinary Tract Infection (UTI). The UTI is the most infectious disease that affects both males and females. This study was designed to detect the bacterial species responsible for UTI in both diabetic and non-diabetic patients in Ma'an province, Jordan. MATERIALS AND METHODS: One hundred sixteen urine samples were investigated to determine UTI-causing bacteria...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023054/genetic-characterization-antimicrobial-resistance-patterns-and-virulence-determinants-of-staphylococcus-aureus-isolated-form-bovine-mastitis
#8
Amal Awad, Hazem Ramadan, Sherif Nasr, Ahmed Ateya, Samar Atwa
BACKGROUND AND OBJECTIVE: Staphylococcus aureus is commonly associated with mastitis in dairy herds with potential public health implications. This study was conducted to investigate the existence of S. aureus in mastitic milk and to determine the antimicrobial resistance profiles of the isolated strains as well as the resistance and virulence associated genes. MATERIALS AND METHODS: Two hundred quarter milk samples were collected from 3 dairy farms at Dakahliya (n = 2) and Damietta (n = 1) Governorates, Egypt from September to December 2016...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29022964/nocardia-arthritidis-keratitis-case-report-and-review-of-the-literature
#9
Andrew Gieger, Stephen Waller, Joseph Pasternak
INTRODUCTION: Keratitis due to Nocardia infection is not commonly encountered in clinical practice and may therefore be mistaken for fungal or viral keratitis leading to delayed treatment and increased risk of permanent visual impairment. CASE: An otherwise healthy 27 years old Caucasian Active Duty military member presented to the clinic with three days of light sensitivity and irritation of his right eye. He carried a history of PRK in both eyes six years prior and admitted to recent contact lens overuse...
January 2017: Nepalese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29022385/effect-of-antibiotic-probiotic-and-human-rotavirus-infection-on-colonisation-dynamics-of-defined-commensal-microbiota-in-a-gnotobiotic-pig-model
#10
H-C Huang, A N Vlasova, A Kumar, S Kandasamy, D D Fischer, L Deblais, F C Paim, S N Langel, M A Alhamo, A Rauf, L Shao, L J Saif, G Rajashekara
We developed a gnotobiotic (Gn) pig model colonised with defined commensal microbiota (DMF) to provide a simplified and controlled system to study the interactions between intestinal commensals, antibiotics (ciprofloxacin, CIP), probiotics (Escherichia coli Nissle 1917, EcN) and virulent human rotavirus (VirHRV). The DMF included seven gut commensal species of porcine origin that mimic the predominant species in the infant gut. Gn piglets were divided into four groups: DMF control (non-treated), DMF+CIP (CIP treated), DMF+CIP+EcN (CIP/EcN treated), DMF+EcN (EcN treated) and inoculated orally with 10(5) cfu of each DMF strain...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29021723/bacterial-infections-in-burn-wound-patients-at-a-tertiary-teaching-hospital-in-accra-ghana
#11
O A Forson, E Ayanka, M Olu-Taiwo, P J Pappoe-Ashong, P J Ayeh-Kumi
Intact human skin surface is essential for protection against infection, preservation of body fluid homeostasis and thermoregulation. Burn injury compromises the skin barrier and enables bacterial infection, hence delaying burn wound healing. This study aimed to determine the microbial profile of burn wounds, and resistance patterns of microbes with respect to the source of the injured patient's wound. Fifty wound swab samples were collected from fifty burn patients at the Korle-Bu Teaching Hospital, Accra (KBTH)...
June 30, 2017: Annals of Burns and Fire Disasters
https://www.readbyqxmd.com/read/29020144/increasing-campylobacter-infections-outbreaks-and-antimicrobial-resistance-in-the-united-states-2004-2012
#12
Aimee L Geissler, Fausto Bustos Carrillo, Krista Swanson, Mary E Patrick, Kathleen E Fullerton, Christy Bennett, Kelly Barrett, Barbara E Mahon
Background: Campylobacteriosis, a leading cause of foodborne illness in the United States, was not nationally notifiable until 2015. Data describing national patterns and trends are limited. We describe the epidemiology of Campylobacter infections in the United States during 2004-2012. Methods: We summarized laboratory-confirmed campylobacteriosis data from the Nationally Notifiable Disease Surveillance System, National Outbreak Reporting System, National Antimicrobial Resistance Monitoring System, and Foodborne Diseases Active Surveillance Network...
July 20, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29019037/role-of-sulfate-chloride-and-nitrate-anions-on-the-degradation-of-fluoroquinolone-antibiotics-by-photoelectro-fenton
#13
Paola Villegas-Guzman, Florian Hofer, Javier Silva-Agredo, Ricardo A Torres-Palma
Taking ciprofloxacin (CIP) as a fluoroquinolone antibiotic model, this work explores the role of common anions (sulfate, nitrate, and chloride) during the application of photoelectro-Fenton (PEF) at natural pH to degrade this type of compound in water. The system was composed of an IrO2 anode, Ti, or gas diffusion electrode (GDE) as cathode, Fe(2+), and UV (254 nm). To determine the implications of these anions, the degradation pathway and efficiency of the PEF sub-processes (UV photolysis, anodic oxidation, and electro-Fenton at natural pH) were studied in the individual presence of the anions...
October 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29018986/hypokalemia-during-antibiotic-treatment-for-bone-and-joint-infections
#14
Carmen Falcone, Leonida Compostella, Antonella Camardo, Li Van Stella Truong, Francesco Centofanti
PURPOSE: During treatment of bone and joint infections (BJIs) with multiple antibiotic therapy, hypokalemia has been reported as a rare side effect. The aim of this study was to evaluate incidence and risk factors for hypokalemia in a cohort of patients treated with multidrug therapy for BJIs, in a single center. METHODS: We retrospectively reviewed 331 clinical files of 150 consecutive patients (65% males; median age 59 years, 95% CI 55-62) admitted repeatedly to our Osteomyelitis Department for treatment of chronic BJIs...
October 10, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/29018810/renaissance-of-conventional-first-line-antibiotics-in-salmonella-enterica-clinical-isolates-assessment-of-mics-for-therapeutic-antimicrobials-in-enteric-fever-cases-from-nepal
#15
Puspa Raj Khanal, Deepa Satyal, Anjeela Bhetwal, Anjila Maharjan, Shreena Shakya, Snehika Tandukar, Narayan Prasad Parajuli
Enteric fever caused by Salmonella enterica is a life-threatening systemic illness of gastrointestinal tract especially in tropical countries. Antimicrobial therapy is generally indicated but resistance towards commonly used antibiotics has limited their therapeutic usefulness. Therefore, we aimed to determine the antimicrobial susceptibility pattern by minimum inhibitory concentration method of common therapeutic regimens against Salmonella enterica from enteric fever clinical cases. Salmonella enterica clinical isolates recovered from the patients with suspected enteric fever whose blood samples were submitted to microbiology laboratory of Manmohan Memorial Community Hospital, Kathmandu, from March 2016 to August 2016, were studied...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29018494/evaluation-of-the-efficacy-and-tolerability-of-oral-ciprofloxacin-used-in-the-comprehensive-treatment-of-external-bacterial-otitis-an-observational-prospective-study
#16
Alexander Vladimirovich Gurov, Andrey Ivanovich Kriukov, Vera Yakovlevna Kunelskaya, Galina Nikolaevna Isotova, Georgiy Borisovich Shadrin, Yuliya Vladislavovna Luchsheva, Vladislav Olegovich Yakimov, Amit Garg, Shyam Prasad Akku, Namita Gupta
Introduction  Otitis Externa is common ear infection with a prevalence of 1%. Objective  The objective of this study is to evaluate the clinical and microbiological efficacy and safety profile with oral ciprofloxacin in the external bacterial otitis (EBO) management. Methods  This is a prospective observational study conducted with EBO outpatients referred to the otorhinolaryngology center in Moscow between March and August 2013. Our study included patients from two cohorts, acute external bacterial otitis (AEBO) - Group 1 - and exacerbation of chronic otitis externa (CEBO) - Group 2...
October 2017: International Archives of Otorhinolaryngology
https://www.readbyqxmd.com/read/29018116/quinolone-resistance-reversion-by-targeting-the-sos-response
#17
E Recacha, J Machuca, P Díaz de Alba, M Ramos-Güelfo, F Docobo-Pérez, J Rodriguez-Beltrán, J Blázquez, A Pascual, J M Rodríguez-Martínez
Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs) and dynamic (killing curves or flow cytometry) methodologies...
October 10, 2017: MBio
https://www.readbyqxmd.com/read/29017452/whole-genome-sequencing-for-the-molecular-characterization-of-carbapenem-resistant-klebsiella-pneumoniae-strains-isolated-at-the-italian-asst-fatebenefratelli-sacco-hospital-2012-2014
#18
Sara Giordana Rimoldi, Bernardina Gentile, Cristina Pagani, Annamaria Di Gregorio, Anna Anselmo, Anna Maria Palozzi, Antonella Fortunato, Valentina Pittiglio, Anna Lisa Ridolfo, Maria Rita Gismondo, Giuliano Rizzardini, Florigio Lista
BACKGROUND: The emergence of carbapenem-resistant Klebsiella pneumoniae strains is threatening antimicrobial treatment. METHODS: Sixty-eight carbapenemase-producing K. pneumoniae strains isolated at Luigi Sacco University Hospital-ASST Fatebenefratelli Sacco (Milan, Italy) between 2012 and 2014 were characterised microbiologically and molecularly. They were tested for drug susceptibility and carbapenemase phenotypes, investigated by means of repetitive extra-genic palindromic polymerase chain reaction (REP-PCR), and fully sequenced by means of next-generation sequencing for the in silico analysis of multi-locus sequence typing (MLST), their resistome, virulome and plasmid content, and their core single nucleotide polymorphism (SNP) genotypes...
October 10, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29017099/co-adsorption-and-sequential-adsorption-of-the-co-existence-four-heavy-metal-ions-and-three-fluoroquinolones-on-the-functionalized-ferromagnetic-3d-nife2o4-porous-hollow-microsphere
#19
Xueyan Liu, Mingyang Liu, Lei Zhang
Functionalized magnetic microsphere NiFe2O4 (MS-NiFe2O4) with a 3D hierarchical porous hollow structure was fabricated using urea as the modifier and soft templates by a simple one-pot solvothermal method. The constructed MS-NiFe2O4 shows excellent dual functions which can not only undergo simultaneous rapid removal of the co-existence metal ions (Cu(2+), Cd(2+), Cr(3+) and Zn(2+) ions), but also availably adsorb fluoroquinolone (FQs) (ciprofloxacin (CIP), enrofloxacin (ENR) and norfloxacin (NOR)), and is easily recycled using an external magnetic field...
September 28, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28994821/chemical-microbial-and-antibiotic-susceptibility-analyses-of-groundwater-after-a-major-flood-event-in-chennai
#20
Ganesan Gowrisankar, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Vetrimurugan Elumalai, Saravanan Dhanamadhavan, Karthikeyan Brindha, Usha Antony, Lakshmanan Elango
During floods, human exposure to pathogens through contaminated water leads to the outbreak of epidemic diseases. This research presents the first extensive assessment of surface and groundwater samples collected immediately after a flood (December 2015) and post-flood (April 2016) from the Adyar River of Chennai, a major city in India, for major ions, trace metals, bacterial population, and pathogens. Severe rains in a short period of time resulted in flooding which inundated the wells, allowing the entry of sewage contaminated river water into the groundwater zone...
October 10, 2017: Scientific Data
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