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Chao Wang, Xue Shi, Lin Liu, Haiyan Li, Jetty S S Ammiraju, David A Kudrna, Wentao Xiong, Hao Wang, Zhaozhao Dai, Yonglian Zheng, Jinsheng Lai, Weiwei Jin, Joachim Messing, Jeffrey L Bennetzen, Rod A Wing, Meizhong Luo
Maize is one of the most important food crops and a key model for genetics and developmental biology. A genetically anchored and high-quality draft genome sequence of maize inbred B73 has been obtained to serve as a reference sequence. To facilitate evolutionary studies in maize and its close relatives, much like the Oryza Map Alignment Project (OMAP) ( bacterial artificial chromosome (BAC) resource did for the rice community, we constructed BAC libraries for maize inbred lines Zheng58, Chang7-2, and Mo17 and maize wild relatives Zea mays ssp...
November 2013: Genetics
Jie Zhang, Zong-Min Shang, Jian-Hua Cao, Bin Fan, Shu-Hong Zhao
In November 2009, scientists from the US, UK, and other countries announced the complete genome sequence draft of the domestic pig. With the release of improved versions of the pig genome assembly and the increase of correctly assembled sequenced fragments over the past two years, it is particularly urgent to have the pig genes annotated at whole-genome level. This article is aimed at introducing an excellent manual annotation tool, Otterlace software, developed by Sanger institute. We used CFL1 (Cofilin 1) gene as an example to expound the usage of the three main components of Otterlace, Zmap, Blixem, and Dotter tools, and developed a practical procedure for manual annotations...
October 2012: Yi Chuan, Hereditas
Jane E Loveland, James G R Gilbert, Ed Griffiths, Jennifer L Harrow
Manual annotation of genomic data is extremely valuable to produce an accurate reference gene set but is expensive compared with automatic methods and so has been limited to model organisms. Annotation tools that have been developed at the Wellcome Trust Sanger Institute (WTSI, are being used to fill that gap, as they can be used remotely and so open up viable community annotation collaborations. We introduce the 'Blessed' annotator and 'Gatekeeper' approach to Community Annotation using the Otterlace/ZMap genome annotation tool...
2012: Database: the Journal of Biological Databases and Curation
Moritz F Wurm, D Yves von Cramon, Ricarda I Schubotz
Action observation engages higher motor areas, possibly reflecting an internal simulation. However, actions considered odd or unusual were found to trigger additional activity in the so-called theory of mind (ToM) network, pointing to deliberations on the actor's mental states. In this functional magnetic resonance imaging study, the hypothesis was tested that an allocentric perspective on a normal action, and even more so the sight of the actor's face, suffices to evoke ToM activity. Subjects observed short videos of object manipulation filmed from either the egocentric or the allocentric perspective, the latter including the actor's face in half of the trials...
December 2011: Human Brain Mapping
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