Yoshie Fujita, Akie Taniguchi, Hanako Yamamoto, Hideru Obinata, Hiroshi Kogo, Akiko Iizuka-Kogo, Maiko Ikezawa, Yukiko Tajika, Satoshi Yokoo, Toshiyuki Matsuzaki
Aquaporin-5 (AQP5) water channel, transmembrane protein 16A (TMEM16A) Ca2+ -activated Cl- channel, and Na+ -K+ -2Cl- cotransporter (NKCC1) are membrane proteins on salivary gland acinar cells that function in watery saliva secretion. We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR)...
February 29, 2024: Acta Histochemica et Cytochemica