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https://www.readbyqxmd.com/read/28214728/lipid-signalling-mediated-by-pld-pa-modulates-proline-and-h2o2-levels-in-barley-seedlings-exposed-to-short-and-long-term-chilling-stress
#1
Micaela Peppino Margutti, Matias Reyna, María Verónica Meringer, Graciela E Racagni, Ana Laura Villasuso
Phospholipase D (PLD) hydrolyses phospholipids to yield phosphatidic acid (PA) and a head group, and is involved in responses to a variety of environmental stresses, including chilling and freezing stress. Barley responses to chilling stress (induced by incubating seedlings at 4 °C) are dynamic and the duration of stress, either short (0-180 min) or long-term (24-36 h) had a significant impact on the response. We investigated the roles of PLD/PA in responses of barley (Hordeum vulgare) seedlings to short and long-term chilling stress, based on regulation of proline and reactive oxygen species (ROS) levels...
February 10, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28210904/acute-and-neuropathic-orofacial-antinociceptive-effect-of-eucalyptol
#2
José de Maria de Albuquerque de Melo Júnior, Marina de Barros Mamede Vidal Damasceno, Sacha Aubrey Alves Rodrigues Santos, Talita Matias Barbosa, João Ronielly Campêlo Araújo, Antonio Eufrásio Vieira-Neto, Deysi Viviana Tenazoa Wong, Roberto César Pereira Lima-Júnior, Adriana Rolim Campos
Terpenes have a wide range of pharmacological properties, including antinociceptive action. The anti-inflammatory and antinociceptive effects of eucalyptol are well established. The purpose of this study was to evaluate the antinociceptive effect of eucalyptol on acute and neuropathic orofacial pain in rodent models. Acute orofacial and corneal nociception was induced with formalin, capsaicin, glutamate and hypertonic saline in mice. In another series, animals were pretreated with capsazepine or ruthenium red to evaluate the involvement of TRPV1 receptors in the effect of eucalyptol...
February 16, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28207974/aamyb1-and-its-orthologue-atmyb61-affect-terpene-metabolism-and-trichome-development-in-artemisia-annua-and-arabidopsis-thaliana
#3
Luis Matías-Hernández, Weimin Jiang, Ke Yang, Kexuan Tang, Peter E Brodelius, Soraya Pelaz
The effective anti-malarial drug artemisinin (AN) isolated from Artemisia annua is relatively expensive due to the low content in the plant as it is only synthesized within the glandular trichomes. Therefore, genetic engineering of A. annua is one of the most promising approaches to improve AN yield. In this work, AaMYB1 transcription factor has been identified and characterized. When AaMYB1 is overexpressed in A. annua, either exclusively in trichomes or in the whole plant, essential AN biosynthetic genes are also overexpressed and consequently AN amount significantly increased...
February 16, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28207968/mri-vs-ultrasound-as-the-initial-imaging-modality-for-pediatric-and-young-adult-patients-with-suspected-appendicitis
#4
Daniel Imler, Christine Keller, Shyam Sivasankar, Nancy Ewen Wang, Shreyas Vasanawala, Matias Bruzoni, James Quinn
BACKGROUND: While ultrasound (US), given its lack of ionizing radiation is currently the recommended initial imaging study of choice for the diagnosis of appendicitis in pediatric and young adult patients, it does have significant shortcomings. US is time intensive, operator dependent, and results in frequent inconclusive studies, thus necessitating further imaging, and admission for observation or repeat clinical visits. A rapid focused Magnetic Resonance Imaging (MRI) for appendicitis has been shown to have definitive sensitivity and specificity, similar to Computed tomography (CT) but without radiation and offers a potential alternative to US...
February 16, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28199423/-outpatient-s-profile-assisted-by-the-psychiatry-residents-of-the-prof-dr-r-rossi-general-hospital-la-plata-argentina
#5
Diego Giannandrea, Julián Achilli Lucas, Lucía Saldaño, María J Barrenengoa, Mariano Dramis, Mariano Barraco, Matías González Olivi, María T Burry
OBJECTIVE: To define the profile and the treatments evolution of the outpatients assisted by the psychiatry residents of the Rossi Hospital in La Plata. METHODS: It was analyzed the period between 2005 and 2010 (six years). The variables selected included gender, age, diagnosis (according to ICD 10), duration and evolution of treatment. RESULTS: From the total number of patients (n=341), 58,7% were women (n= 200) and 41,3% men (n= 141). The most frequent diagnoses were: anxiety disorders (13,6%), depressive disorders (12,8%), schizophrenia (12,2%), other non-affective psychoses (9%) and mental retardation (8,4%)...
March 2016: Vertex: Revista Argentina de Psiquiatriá
https://www.readbyqxmd.com/read/28198569/effect-of-eicosapentaenoic-acid-on-bovine-cumulus-oocyte-complex-in-vitro
#6
Noelia Nikoloff, Ana M Pascua, Juan M Anchordoquy, Juan P Anchordoquy, Matías A Sirini, Analia Seoane, Cecilia C Furnus
The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM and 1 mM EPA. The effect of EPA was evaluated on cumulus-oocyte complexes (COC) by oocyte maturation [cumulus expansion area and oocyte nuclear maturation], genotoxicity [single cell gel electrophoresis (SCGE)] and cytotoxicity [apoptosis, viability and MTT assays] end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVM medium...
February 15, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28193664/in-vitro-antiviral-activity-and-resistance-profile-of-the-next-generation-hepatitis-c-virus-ns5a-inhibitor-pibrentasvir
#7
Teresa I Ng, Preethi Krishnan, Tami Pilot-Matias, Warren Kati, Gretja Schnell, Jill Beyer, Thomas Reisch, Liangjun Lu, Tatyana Dekhtyar, Michelle Irvin, Rakesh Tripathi, Clarence Maring, John T Randolph, Rolf Wagner, Christine Collins
Pibrentasvir (ABT-530) is a novel and pan-genotypic hepatitis C virus (HCV) NS5A inhibitor with 50% effective concentration (EC50)values ranging from 1.4 to 5.0 picomolar (pM) against HCV replicons containing NS5A from genotypes 1 to 6. Pibrentasvir demonstrated similar activity against a panel of chimeric replicons containing HCV genotype 1 to 6 NS5A from clinical samples. Resistance selection studies were conducted using HCV replicon cells with NS5A from genotype 1a, 1b, 2a, 2b, 3a, 4a, 5a or 6a at pibrentasvir concentration of 10- or 100-fold over its EC50 values for the respective replicons...
February 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28193534/implementation-of-new-tools-in-molecular-epidemiology-studies-of-echinococcus-granulosus-sensu-lato-in-south-america
#8
Héctor G Avila, Guilherme B Santos, Marcela A Cucher, Natalia Macchiaroli, Matías G Pérez, Germán Baldi, Oscar Jensen, Verónica Pérez, Raúl López, Perla Negro, Exequiel Scialfa, Arnaldo Zaha, Henrique B Ferreira, Mara Rosenzvit, Laura Kamenetzky
The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting analysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination...
February 10, 2017: Parasitology International
https://www.readbyqxmd.com/read/28188835/automated-pulse-discrimination-of-two-freely-swimming-weakly-electric-fish-and-analysis-of-their-electrical-behavior-during-a-dominance-contest
#9
Rafael T Guariento, Thiago S Mosqueiro, Paulo Matias, Vinicius B Cesarino, Lirio O B Almeida, Jan F W Slaets, Leonardo P Maia, Reynaldo D Pinto
Electric fishes modulate their electric organ discharges with a remarkable variability. Some patterns can be easily identified, such as pulse rate changes, offs and chirps, which are often associated with important behavioral contexts, including aggression, hiding and mating. However, these behaviors are only observed when at least two fish are freely interacting. Although their electrical pulses can be easily recorded by non-invasive techniques, discriminating the emitter of each pulse is challenging when physically similar fish are allowed to freely move and interact...
February 7, 2017: Journal of Physiology, Paris
https://www.readbyqxmd.com/read/28186004/rich-stochastic-dynamics-of-co-doped-er-yb-fluorescence-upconversion-nanoparticles-in-the-presence-of-thermal-non-conservative-harmonic-and-optical-forces
#10
Rene A Nome, Cecilia Sorbello, Matías Jobbágy, Beatriz C Barja, Vitor Sanches, Joyce S Cruz, Vinicius F Aguiar
The stochastic dynamics of individual co-doped Er:Yb upconversion nanoparticles (UCNP) were investigated from experiments and simulations. The UCNP were characterized by high-resolution scanning electron microscopy, dynamic light scattering, and zeta potential measurements. Single UCNP measurements were performed by fluorescence upconversion micro-spectroscopy and optical trapping. The mean-square displacement (MSD) from single UCNP exhibited a time-dependent diffusion coefficient which was compared with Brownian dynamics simulations of a viscoelastic model of harmonically bound spheres...
February 10, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28184357/delayed-splenic-rupture-normal-appearing-spleen-on-the-initial-multidetector-computed-tomography-mdct-can-sometimes-be-misleading
#11
Alireza Hamidian Jahromi, Matias Migliaro, Melisa Romano, Guillermo Sangster
INTRODUCTION: Delayed splenic rupture (DSR) is an unusual outcome following blunt abdominal trauma. Although DSR is defined as bleeding more than 48 hours after blunt trauma in a previously hemodynamically stable patient, a review of the reported cases in the literature shows that in almost all of the cases the initial CT imaging revealed some form of damage to the spleen. CASE PRESENTATION: Here we describe an extremely rare condition in a case that presented with a DSR following blunt trauma and had a normal appearing spleen in the initial post trauma MDCT scan...
November 2016: Trauma Monthly
https://www.readbyqxmd.com/read/28183850/deltex-1-mutations-predict-poor-survival-in-diffuse-large-b-cell-lymphoma
#12
Leo Meriranta, Annika Pasanen, Riku Louhimo, Alejandra Cervera, Amjad Alkodsi, Matias Autio, Minna Taskinen, Ville Rantanen, Marja-Liisa Karjalainen-Lindsberg, Harald Holte, Jan Delabie, Rainer Lehtonen, Sampsa Hautaniemi, Sirpa Leppä
No abstract text is available yet for this article.
February 9, 2017: Haematologica
https://www.readbyqxmd.com/read/28177295/stunting-of-the-penis-in-heleobia-parchappii-mollusca-cochliopidae-and-its-relationship-with-parasitism
#13
Matías J Merlo, Manuela Parietti, Jorge A Etchegoin
Penis anatomy is used to discriminate species of gastropods belonging to the family Cochliopidae; however, this characteristic may be affected by the presence of parasites. To evaluate the possible effect of parasites on penis length and number of papillae in Heleobia parchappii, 195 males were collected from the Nahuel Rucá Lagoon, Argentina. Male snails were only infected by trematode digeneans (total prevalence 45.13%). Three out of 9 species of digeneans registered showed prevalence values higher than 10%: Microphallus szidati, M...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28168347/local-and-systemic-diffusion-of-antineoplastic-drugs-following-vertebroplasty-using-acrylic-cement-mixed-with-cisplatin-or-methotrexate-experimental-study-in-pigs
#14
Rafael Llombart-Blanco, Carlos Villas, Álvaro Silva, Azucena Aldaz, Iñigo Navarro, Jeronimo Forteza, Salvador Martin Algarra, Matías Alfonso
PURPOSE: To determine the efficacy of cisplatin- or methotrexate-containing acrylic cement for local and systemic antineoplastic drug diffusion. Among the uses of acrylic cement or Polymethylmethacrylate (PMMA), there is the possibility to employ it as vehicle for drug diffusion. This capability is of interest in the treatment of pathological fractures: The curative effects of the cement (cytotoxicity of the monomer and increased temperature) are added to the antineoplastic effect of the drugs...
February 6, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28167134/from-molecule-to-behavior-brain-aromatase-cyp19a1b-characterization-expression-analysis-and-its-relation-with-social-status-and-male-agonistic-behavior-in-a-neotropical-cichlid-fish
#15
Martín R Ramallo, Leonel Morandini, Agustina Birba, Gustavo M Somoza, Matías Pandolfi
The enzyme aromatase, responsible for the conversion of C19 androgens to C18 estrogens, exists as two paralogue copies in teleost fish: Cyp19a1a mostly expressed in the gonads, referred as gonadal aromatase, and Cyp19a1b, mostly expressed in the brain, accordingly known as brain aromatase. The neural localization of Cyp19a1b is greatly contained within the social behavior network and mesolimbic reward system in fish, suggesting a strong role of estrogen synthesis in the regulation of social behavior. In this work we aimed to analyze the variation in cyp19a1b expression in brain and pituitary of males of a highly social cichlid, Cichlasoma dimerus (locally known as chanchita), and its relation with inter-individual variability in agonistic behavior in a communal social environment...
February 3, 2017: Hormones and Behavior
https://www.readbyqxmd.com/read/28165339/mutations-in-sphingosine-1-phosphase-lyase-cause-nephrosis-with-ichthyosis-and-adrenal-insufficiency
#16
Svjetlana Lovric, Sara Goncalves, Heon Yung Gee, Babak Oskouian, Honnappa Srinivas, Won-Il Choi, Shirlee Shril, Shazia Ashraf, Weizhen Tan, Jia Rao, Merlin Airik, David Schapiro, Daniela A Braun, Carolin E Sadowski, Eugen Widmeier, Tilman Jobst-Schwan, Johanna Magdalena Schmidt, Vladimir Girik, Guido Capitani, Jung H Suh, Noëlle Lachaussée, Christelle Arrondel, Julie Patat, Olivier Gribouval, Monica Furlano, Olivia Boyer, Alain Schmitt, Vincent Vuiblet, Seema Hashmi, Rainer Wilcken, Francois P Bernier, A Micheil Innes, Jillian S Parboosingh, Ryan E Lamont, Julian P Midgley, Nicola Wright, Jacek Majewski, Martin Zenker, Franz Schaefer, Navina Kuss, Johann Greil, Thomas Giese, Klaus Schwarz, Vilain Catheline, Denny Schanze, Ingolf Franke, Yves Sznajer, Anne S Truant, Brigitte Adams, Julie Désir, Ronald Biemann, York Pei, Elisabet Ars, Nuria Lloberas, Alvaro Madrid, Vikas R Dharnidharka, Anne M Connolly, Marcia C Willing, Megan A Cooper, Richard P Lifton, Matias Simons, Howard Riezman, Corinne Antignac, Julie D Saba, Friedhelm Hildebrandt
Steroid-resistant nephrotic syndrome (SRNS) causes 15% of chronic kidney disease cases. A mutation in 1 of over 40 monogenic genes can be detected in approximately 30% of individuals with SRNS whose symptoms manifest before 25 years of age. However, in many patients, the genetic etiology remains unknown. Here, we have performed whole exome sequencing to identify recessive causes of SRNS. In 7 families with SRNS and facultative ichthyosis, adrenal insufficiency, immunodeficiency, and neurological defects, we identified 9 different recessive mutations in SGPL1, which encodes sphingosine-1-phosphate (S1P) lyase...
February 6, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28160854/the-anti-inflammatory-effects-of-n-methyl-2s-4r-trans-4-hydroxy-l-proline-from-syderoxylon-obtusifolium-are-related-to-its-inhibition-of-tnf-alpha-and-inflammatory-enzymes
#17
Pedro Everson Alexandre de Aquino, Talita Rocha Magalhães, Lucas Antônio Duarte Nicolau, Luzia Kalyne A Moreira Leal, Nayara Coriolano de Aquino, Sabrina Matias Dos Santos, Kelly Rose Tavares Neves, Edilberto Rocha Silveira, Glauce Socorro de Barros Viana
BACKGROUND: Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn., Sapotaceae family, is a medicinal species native to the Brazilian Northeastern region. The plant is popularly used as an anti-inflammatory and hypoglycemic. PURPOSE: To evaluate the anti-inflammatory properties of the N-methyl-(2S,4R)-trans-4-hydroxy-l-proline (NMP) from S. obtusifolium leaves in models of inflammation and to clarify its action mechanisms. METHODS: Male Swiss mice were distributed intocontrols and groups treated with NMP (25, 50 and 100mg/kg, p...
January 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28160495/nmda-receptor-subunit-composition-controls-dendritogenesis-of-hippocampal-neurons-through-camkii-creb-p-and-h3k27ac
#18
Fernando J Bustos, Nur Jury, Pablo Martinez, Estibaliz Ampuero, Matias Campos, Sebastian Abarzúa, Karen Jaramillo, Susanne Ibing, Muriel D Mardones, Henny Haensgen, Julia Kzhyshkowska, Maria Florencia Tevy, Rachael Neve, Magdalena Sanhueza, Lorena Varela-Nallar, Martín Montecino, Brigitte van Zundert
Dendrite arbor growth, or dendritogenesis, is choreographed by a diverse set of cues, including the NMDA receptor (NMDAR) subunits NR2A and NR2B. While NR1NR2B receptors are predominantly expressed in immature neurons and promote plasticity, NR1NR2A receptors are mainly expressed in mature neurons and induce circuit stability. How the different subunits regulate these processes is unclear, but this is likely related to the presence of their distinct C-terminal sequences that couple different signaling proteins...
February 4, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28160166/depletion-of-abscisic-acid-levels-in-roots-of-flooded-carrizo-citrange-poncirus-trifoliata-l-raf-%C3%A3-citrus-sinensis-l-osb-plants-is-a-stress-specific-response-associated-to-the-differential-expression-of-pyr-pyl-rcar-receptors
#19
Vicent Arbona, Sara I Zandalinas, Matías Manzi, Miguel González-Guzmán, Pedro L Rodriguez, Aurelio Gómez-Cadenas
Soil flooding reduces root abscisic acid (ABA) levels in citrus, conversely to what happens under drought. Despite this reduction, microarray analyses suggested the existence of a residual ABA signaling in roots of flooded Carrizo citrange seedlings. The comparison of ABA metabolism and signaling in roots of flooded and water stressed plants of Carrizo citrange revealed that the hormone depletion was linked to the upregulation of CsAOG, involved in ABA glycosyl ester (ABAGE) synthesis, and to a moderate induction of catabolism (CsCYP707A, an ABA 8'-hydroxylase) and buildup of dehydrophaseic acid (DPA)...
February 3, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28157983/visibility-simulation-of-realistic-grating-interferometers-including-grating-geometries-and-energy-spectra
#20
Ralph P Harti, Christian Kottler, Jacopo Valsecchi, Konstantins Jefimovs, Matias Kagias, Markus Strobl, Christian Grünzweig
The performance of X-ray and neutron grating interferometers is characterised by their visibility, which is a measure for the maximum achievable contrast. In this study we show how the real grating geometry in a grating interferometer with three gratings impacts the interference and self projection that leads to visibility in the first place. We quantify the individual contributions of wavelength distributions and grating shapes in terms of visibility reduction by determining the absolute as well as relative effect of each contribution...
January 23, 2017: Optics Express
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