Godhev Kumar Manakkat Vijay, Ming Zhou, Kairavee Thakkar, Abigail Rothrauff, Amanpreet Singh Chawla, Dianyu Chen, Louis Chi-Wai Lau, Peter Habib Gerges, Kashish Chetal, Prabal Chhibbar, Jingyu Fan, Jishnu Das, Alok Joglekar, Lisa Borghesi, Nathan Salomonis, Heping Xu, Harinder Singh
Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Here, using a model antigen in mice, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using single-cell RNA sequencing and B cell antigen receptor sequencing in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveals a new PC transition state that gives rise to functionally competent PC precursors...
May 2, 2024: Nature Immunology