keyword
MENU ▼
Read by QxMD icon Read
search

Mgam

keyword
https://www.readbyqxmd.com/read/28500854/systematic-analysis-of-coronary-artery-disease-datasets-revealed-the-potential-biomarker-and-treatment-target
#1
Yan Shi, Sijin Yang, Man Luo, Wei-Dong Zhang, Zun-Ping Ke
Coronary artery disease caused about 1 of every 7 deaths in the United States and early prevention was potential to decrease the incidence and mortality. We aimed to figure the genes involving in the coronary artery disease using meta-anlaysis. Five datasets of coronary heart disease from GEO series were retrieved and data preprocessing and quality control were carried out. Moderated t-test was used to decide the differentially expressed genes for a single dataset. And the combined p-value using systematic-analysis methods were conducted using MetaDE...
April 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28443839/phenolic-compounds-increase-the-transcription-of-mouse-intestinal-maltase-glucoamylase-and-sucrase-isomaltase
#2
Meric Simsek, Roberto Quezada-Calvillo, Buford L Nichols, Bruce R Hamaker
Diverse natural phenolic compounds show inhibition activity of intestinal α-glucosidases, which may constitute the molecular basis for their ability to control systemic glycemia. Additionally, phenolics can modify mRNA expression for proteins involved in nutritional, metabolic or immune processes. To explore the possibility that phenolics can regulate the mRNA expression, enzymatic activity, and protein synthesis/processing of intestinal Maltase-Glucoamylase (MGAM) and Sucrase-Isomaltase (SI), small intestinal explants from Balb/c mice were cultured for 24 h in the presence or absence of gallic acid, caffeic acid, and (+)-catechin at 0...
April 26, 2017: Food & Function
https://www.readbyqxmd.com/read/28339792/interference-of-age-and-supplementation-of-direct-fed-microbial-and-essential-oil-in-the-activity-of-digestive-enzymes-and-expression-of-genes-related-to-transport-and-digestion-of-carbohydrates-and-proteins-in-the-small-intestine-of-broilers
#3
M F Fernandez-Alarcon, N Trottier, J P Steibel, R Lunedo, D M B Campos, A M Santana, J M Pizauro, R L Furlan, L R Furlan
The objectives of this study were to describe alterations that age and dietary inclusion of direct-fed microbial (DFM) Bacillus subtilis (BS) and a specific essential oil (EO) blend (carvacrol, cinnamaldehyde, cineol, and pepper extract) causes in the activity of digestive enzymes (maltase: MALT; aminopeptidase-N: APN; intestinal alkaline phosphate: IAP) and expression patterns of genes related to transport (oligopeptide transporter gene: SLC15A1; Na+-dependent glucose and galactose transporter gene: SLC5A1; Na+-independent glucose, galactose, and fructose transporter gene: SLC2A2; ATPase, Na+/K+ transporting gene: ATP1A1) and digestion (aminopeptidase-N gene: ANPEP; maltase-glucoamylase gene: MGAM; Sucrase-isomaltase gene: SI) of carbohydrates and proteins in the small intestine of broilers...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28321889/antihyperglycaemic-activity-of-the-methanol-extract-from-leaves-of-eremophila-maculata-scrophulariaceae-in-streptozotocin-induced-diabetic-rats
#4
Fadia S Youssef, Mohamed L Ashour, Sherif S Ebada, Mansour Sobeh, Hesham A El-Beshbishy, Abdel Nasser Singab, Michael Wink
OBJECTIVES: This study was designed to evaluate the antihyperglycaemic activity of the methanol leaf extract of Eremophila maculata (EMM) both in vitro and in vivo. METHODS: The antihyperglycaemic activity was assessed in vitro using differentiated 3T3-L1 adipocytes, whereas in-vivo effect was evaluated in streptozotocin-induced diabetic rats. Chemical profiling of EMM was done using LC-ESI-MS techniques. Molecular modelling experiments of the identified compounds were performed using C-Docker protocol...
June 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28267073/improved-starch-digestion-of-sucrase-deficient-shrews-treated-with-oral-glucoamylase-enzyme-supplements
#5
Buford L Nichols, Stephen E Avery, Roberto Quezada-Calvillo, Shadi B Kilani, Amy Hui-Mei Lin, Douglas G Burrin, Benjamin E Hodges, Shaji K Chacko, Antone R Opekun, Marwa El Hindawy, Bruce R Hamaker, Sen-Ichi Oda
Although named because of its sucrose hydrolytic activity, this mucosal enzyme plays a leading role in starch digestion because of its maltase and glucoamylase activities. Sucrase deficient mutant shrews, Suncus murinus, were used as a model to investigate starch digestion in patients with Congenital Sucrase-isomaltase Deficiency (CSID). BACKGROUND: Starch digestion is much more complex than sucrose digestion. Six enzyme activities, two α-amylases (Amy) and four mucosal α-glucosidases (maltases), including maltase-glucoamylase (Mgam) and sucrase-isomaltase (Si) subunit activities, are needed to digest starch to absorbable free glucose...
March 6, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/27312099/human-glioblastoma-associated-microglia-monocytes-express-a-distinct-rna-profile-compared-to-human-control-and-murine-samples
#6
Frank Szulzewsky, Sonali Arora, Lot de Witte, Thomas Ulas, Darko Markovic, Joachim L Schultze, Eric C Holland, Michael Synowitz, Susanne A Wolf, Helmut Kettenmann
Glioblastoma (GBM) is the most aggressive brain tumor in adults. It is strongly infiltrated by microglia and peripheral monocytes that support tumor growth. In the present study we used RNA sequencing to compare the expression profile of CD11b(+) human glioblastoma-associated microglia/monocytes (hGAMs) to CD11b(+) microglia isolated from non-tumor samples. Hierarchical clustering and principal component analysis showed a clear separation of the two sample groups and we identified 334 significantly regulated genes in hGAMs...
August 2016: Glia
https://www.readbyqxmd.com/read/26319403/natural-selection-at-the-brush-border-adaptations-to-carbohydrate-diets-in-humans-and-other-mammals
#7
Chiara Pontremoli, Alessandra Mozzi, Diego Forni, Rachele Cagliani, Uberto Pozzoli, Giorgia Menozzi, Jacopo Vertemara, Nereo Bresolin, Mario Clerici, Manuela Sironi
Dietary shifts can drive molecular evolution in mammals and a major transition in human history, the agricultural revolution, favored carbohydrate consumption. We investigated the evolutionary history of nine genes encoding brush-border proteins involved in carbohydrate digestion/absorption. Results indicated widespread adaptive evolution in mammals, with several branches experiencing episodic selection, particularly strong in bats. Many positively selected sites map to functional protein regions (e.g., within glucosidase catalytic crevices), with parallel evolution at SI (sucrase-isomaltase) and MGAM (maltase-glucoamylase)...
September 2015: Genome Biology and Evolution
https://www.readbyqxmd.com/read/26162745/modeling-of-cooked-starch-digestion-process-using-recombinant-human-pancreatic-%C3%AE-amylase-and-maltase-glucoamylase-for-in%C3%A2-vitro-evaluation-of-%C3%AE-glucosidase-inhibitors
#8
Xiaofang Cao, Chen Zhang, Yangyang Dong, Peng Geng, Fang Bai, Gang Bai
In human, digestion of cooked starch mainly involves breaking down of α-amylase to α-limit dextrins and small linear malto-oligosaccharides, which are in turn hydrolyzed to glucose by the gut mucosal maltase-glucoamylase (MGAM). Human pancreatic α-amylase (HPA), amino- and carboxyl-terminal portions of MGAM (ntMGAM and ctMGAM) catalyze the hydrolysis of α-D-(1,4) glycosidic linkages in starch, playing a crucial role in the production of glucose in the human lumen. Accordingly, these enzymes are effective drug targets for the treatments of type 2 diabetes and obesity...
September 23, 2015: Carbohydrate Research
https://www.readbyqxmd.com/read/25816913/dietary-phenolic-compounds-selectively-inhibit-the-individual-subunits-of-maltase-glucoamylase-and-sucrase-isomaltase-with-the-potential-of-modulating-glucose-release
#9
Meric Simsek, Roberto Quezada-Calvillo, Mario G Ferruzzi, Buford L Nichols, Bruce R Hamaker
In this study, it was hypothesized that dietary phenolic compounds selectively inhibit the individual C- and N-terminal (Ct, Nt) subunits of the two small intestinal α-glucosidases, maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI), for a modulated glycemic carbohydrate digestion. The inhibition by chlorogenic acid, caffeic acid, gallic acid, (+)-catechin, and (-)-epigallocatechin gallate (EGCG) on individual recombinant human Nt-MGAM and Nt-SI and on mouse Ct-MGAM and Ct-SI was assayed using maltose as the substrate...
April 22, 2015: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/25776870/a-genome-wide-study-of-inherited-deletions-identified-two-regions-associated-with-nonsyndromic-isolated-oral-clefts
#10
Samuel G Younkin, Robert B Scharpf, Holger Schwender, Margaret M Parker, Alan F Scott, Mary L Marazita, Terri H Beaty, Ingo Ruczinski
BACKGROUND: DNA copy number variants play an important part in the development of common birth defects such as oral clefts. Individual patients with multiple birth defects (including oral clefts) have been shown to carry small and large chromosomal deletions. METHODS: We investigated the role of polymorphic copy number deletions by comparing transmission rates of deletions from parents to offspring in case-parent trios of European ancestry ascertained through a cleft proband with trios ascertained through a normal offspring...
April 2015: Birth Defects Research. Part A, Clinical and Molecular Teratology
https://www.readbyqxmd.com/read/25154971/inhibitory-effects-of-pomegranate-extracts-on-recombinant-human-maltase-glucoamylase
#11
Kayoko Kawakami, Peng Li, Misugi Uraji, Tadashi Hatanaka, Hideyuki Ito
α-Glucosidase inhibitors are currently used in the treatment of type 2 diabetes. In this study, we investigated the inhibitory activities of aril and pericarp extracts from pomegranates obtained various regions against recombinant human maltase-glucoamylase (MGAM). The inhibitory activities of the aril extracts tended to be stronger than those of the pericarp extracts. The Iranian aril extract was the most effective inhibitor. We investigated the polyphenol content of the pomegranate extracts using the Folin-Ciocalteu method...
September 2014: Journal of Food Science
https://www.readbyqxmd.com/read/25058599/gene-expression-profile-analysis-for-different-idiopathic-interstitial-pneumonias-subtypes
#12
Hanzhang Chen, Xia Fang, Hailong Zhu, Shuai Li, Jian He, Pan Gu, Deshen Fan, Fei Han, Yu Zeng, Xiaotin Yu, Benfang Luo, Haodong Xu, Xianghua Yi
OBJECTIVE: Idiopathic interstitial pneumonias (IIPs) are a group of diffuse parenchymal lung diseases of unknown etiology characterized by the presence of various degrees of inflammation and fibrosis. We aimed to screen the differences among IIPs subtypes in the gene level by using the microarray expression profiles of normal lung tissue and IIPs tissue for the key genes associated with early diagnosis and treatment of IIPs. METHODS: The gene expression profile of six kinds of IIPs (GSE 32537) subtypes tissue and normal lung tissues were downloaded...
October 2014: Experimental Lung Research
https://www.readbyqxmd.com/read/25037326/branch-pattern-of-starch-internal-structure-influences-the-glucogenesis-by-mucosal-nt-maltase-glucoamylase
#13
Amy Hui-Mei Lin, Zihua Ao, Roberto Quezada-Calvillo, Buford L Nichols, Chi-Tien Lin, Bruce R Hamaker
To produce sufficient amounts of glucose from food starch, both α-amylase and mucosal α-glucosidases are required. We found previously that the digestion rate of starch is influenced by its susceptibility to mucosal α-glucosidases. In the present study, six starches and one glycogen were pre-hydrolyzed by α-amylase for various time periods, and then further hydrolyzed with the mucosal α-glucosidase, the N-terminal subunit of maltase-glucoamylase (Nt-MGAM), to generate free glucose. Results showed that α-amylase amplified the Nt-MGAM glucogenesis, and that the amplifications differed in various substrates...
October 13, 2014: Carbohydrate Polymers
https://www.readbyqxmd.com/read/24944015/improvement-of-stability-of-nitrile-hydratase-via-protein-fragment-swapping
#14
Youtian Cui, Wenjing Cui, Zhongmei Liu, Li Zhou, Michihiko Kobayashi, Zhemin Zhou
Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, is the key enzyme for the production of amides in industries. However, the poor stability of this enzyme under the reaction conditions is a drawback of its industrial application. In this study, we aimed to improve the stability of NHase (PpNHase) from Pseudomonas putida NRRL-18668 using a homologous protein fragment swapping strategy. One thermophilic NHase fragment from Comamonas testosteroni 5-MGAM-4D and two fragments from Pseudonocardia thermophila JCM3095 were selected to swap the corresponding fragments of PpNHase...
July 18, 2014: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/24377104/an-integrated-strategy-of-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-and-virtual-screening-for-the-identification-of-%C3%AE-glucosidase-inhibitors-in-acarviostatin-containing-complex
#15
Liqiang Wanga, Qingxin Cui, Yuanyuan Hou, Fang Bai, Jixue Sun, Xiaofang Cao, Pi Liu, Min Jiang, Gang Bai
We propose a strategy that integrates ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) and virtual docking to identify inhibitors of multiple human -glucosidases. UPLC yielded AIB656, an acarviostatin-containing complex, which was analyzed by Q-TOF-MS to acquire structural information and was tested for inhibition of N-terminal (MGAM-N), C-terminal (MGAM-C) catalytic domain of maltase-glucoamylase, and human pancreatic -amylase (HPA).A systematic computational study was performed to evaluate the inhibition activity for 51 identified acarviostatins with various sizes, including trace or difficult-to-prepare ingredients...
December 6, 2013: Journal of Chromatography. A
https://www.readbyqxmd.com/read/24046257/probing-the-binding-of-syzygium-derived-%C3%AE-glucosidase-inhibitors-with-n-and-c-terminal-human-maltase-glucoamylase-by-docking-and-molecular-dynamics-simulation
#16
Debasish Roy, Vinod Kumar, Kshitish K Acharya, Kavitha Thirumurugan
Human maltase glucoamylase (MGAM) is a potent molecular target for controlling post prandial glucose surplus in type 2 diabetes. Binding of small molecules from Syzygium sp. with α-glucosidase inhibitory potential in MGAM has been investigated in silico. Our results suggest that myricetin was the most potent inhibitor with high binding affinity for both N- and C-terminals of MGAM. Molecular dynamics revealed that myricetin interacts in its stretched conformation through water-mediated interactions with C-terminal of MGAM and by normal hydrogen bonding with the N-terminal...
January 2014: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/23964564/naturally-occurring-sulfonium-ion-glucosidase-inhibitors-and-their-derivatives-a-promising-class-of-potential-antidiabetic-agents
#17
REVIEW
Sankar Mohan, Razieh Eskandari, B Mario Pinto
In humans, four different enzymes mediate the digestion of ingested carbohydrates. First salivary and pancreatic α-amylases, the two endoacting retaining glucosidases, break down the complex starch molecules into smaller linear maltose-oligomers (LM) and branched α-limit dextrins (αLDx). Then two retaining exoglucosidases, maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI), convert those molecules into glucose in the small intestine. The small intestinal brush-border epithelial cells anchor MGAM and SI, and each contains a catalytic N- and C-terminal subunit, ntMGAM, ctMGAM, ntSI, and ctSI, respectively...
January 21, 2014: Accounts of Chemical Research
https://www.readbyqxmd.com/read/23924158/candidate-serum-biomarkers-for-early-intestinal-cancer-using-15n-metabolic-labeling-and-quantitative-proteomics-in-the-apcmin-mouse
#18
Melanie M Ivancic, Edward L Huttlin, Xiaodi Chen, Jennifer K Pleiman, Amy A Irving, Adrian D Hegeman, William F Dove, Michael R Sussman
Current screening procedures for colorectal cancer are imperfect and highly invasive and result in increased mortality rates due to low compliance. The goal of the experiments reported herein is to identify potential blood-based biomarkers indicative of early stage intestinal cancers using the ApcMin/+ mouse model of intestinal cancer as an experimental system. Serum proteins from tumor-bearing ApcMin/+ mice were quantitatively compared to tumor-free Apc+/+ wild-type mice via in anima metabolic labeling with 14N/15N-labeled Spirulina algae and an LTQ Orbitrap mass spectrometer...
September 6, 2013: Journal of Proteome Research
https://www.readbyqxmd.com/read/23838818/maltase-glucoamylase-modulates-gluconeogenesis-and-sucrase-isomaltase-dominates-starch-digestion-glucogenesis
#19
Maricela Diaz-Sotomayor, Roberto Quezada-Calvillo, Stephen E Avery, Shaji K Chacko, Li-Ke Yan, Amy Hui-Mei Lin, Zi-hua Ao, Bruce R Hamaker, Buford L Nichols
OBJECTIVES: Six enzyme activities are needed to digest starch to absorbable free glucose; 2 luminal α-amylases (AMY) and 4 mucosal maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) subunit activities are involved in the digestion. The AMY activities break down starch to soluble oligomeric dextrins; mucosal MGAM and SI can either directly digest starch to glucose or convert the post-α-amylolytic dextrins to glucose. We hypothesized that MGAM, with higher maltase than SI, drives digestion on ad limitum intakes and SI, with lower activity but more abundant amount, constrains ad libitum starch digestion...
December 2013: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/23637978/applied-mixed-generalized-additive-model-to-assess-the-effect-of-temperature-on-the-incidence-of-bacillary-dysentery-and-its-forecast
#20
Weiping Ma, Xiaodong Sun, Yanyan Song, Fangfang Tao, Wei Feng, Yi He, Naiqing Zhao, Zhengan Yuan
BACKGROUND: Association between bacillary dysentery (BD) disease and temperature has been reported in some studies applying Poisson regression model, however the effect estimation might be biased due to the data autocorrelation. Furthermore the temperature effect distributed in the time of different lags has not been studied either. The purpose of this work was to obtaining the association between the BD counts and the climatic factors such as temperature in the form of the weighted averages, concerning the autocorrelation pattern of the model residuals, and to make short term predictions using the model...
2013: PloS One
keyword
keyword
18484
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"