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https://www.readbyqxmd.com/read/28444728/circulating-cell-free-braf-v600e-as-a-biomarker-in-children-with-langerhans-cell-histiocytosis
#1
Sébastien Héritier, Zofia Hélias-Rodzewicz, Hélène Lapillonne, Nathalie Terrones, Sonia Garrigou, Corinne Normand, Mohamed-Aziz Barkaoui, Jean Miron, Geneviève Plat, Nathalie Aladjidi, Anne Pagnier, Anne Deville, Marion Gillibert-Yvert, Despina Moshous, Alain Lefèvre-Utile, Anne Lutun, Catherine Paillard, Caroline Thomas, Eric Jeziorski, Philippe Nizard, Valérie Taly, Jean-François Emile, Jean Donadieu
The BRAF(V600E) mutation is reported in half of patients with Langerhans cell histiocytosis (LCH). This study investigated the detection of the BRAF(V600E) allele in circulating cell-free (ccf) DNA in a paediatric LCH cohort. Children with BRAF(V600E) -mutated LCH were investigated to detect ccf BRAF(V600E) at diagnosis (n = 48) and during follow-up (n = 17) using a picolitre-droplet digital PCR assay. At diagnosis, ccf BRAF(V600E) was positive in 15/15 (100%) patients with risk-organ positive multisystem (RO+ MS) LCH, 5/12 (42%) of patients with RO- MS LCH and 3/21 (14%) patients with single-system (SS) LCH (P < 0·001, Fisher's exact test)...
April 25, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28417651/laparoscopic-management-of-cholecystoenteric-fistula-a-single-center-experience
#2
Xiang-Yang Li, Xin Zhao, Peng Zheng, Xiao-Ming Kao, Xiao-Song Xiang, Wu Ji
Aim To report our experience regarding management of cholecystoenteric fistula (CEF) and identify the most effective diagnostic methods and surgical treatment. Methods In total, 10,588 patients underwent laparoscopic cholecystectomy for cholecystolithiasis from January 2000 to December 2014 at the Research Institute of General Surgery, Jinling Hospital (Nanjing, China). Twenty-nine patients were diagnosed with CEF preoperatively or intraoperatively. Data were retrospectively collected on demographics, preoperative diagnostics, intraoperative findings, laparoscopic procedures, complications, and follow-up...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28399962/time-lag-between-immigration-and-tuberculosis-rates-in-immigrants-in-the-netherlands-a-time-series-analysis
#3
C van Aart, H Boshuizen, A Dekkers, H Korthals Altes
SETTING AND OBJECTIVE: In low-incidence countries, most tuberculosis (TB) cases are foreign-born. We explored the temporal relationship between immigration and TB in first-generation immigrants between 1995 and 2012 to assess whether immigration can be a predictor for TB in immigrants from high-incidence countries. DESIGN: We obtained monthly data on immigrant TB cases and immigration for the three countries of origin most frequently represented among TB cases in the Netherlands: Morocco, Somalia and Turkey...
May 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28323084/integration-of-in-hospital-cardiac-arrest-contextual-curriculum-into-a-basic-life-support-course-a-randomized-controlled-simulation-study
#4
Elizabeth A Hunt, Jordan M Duval-Arnould, Nnenna O Chime, Kareen Jones, Michael Rosen, Merona Hollingsworth, Deborah Aksamit, Marida Twilley, Cheryl Camacho, Daniel P Nogee, Julianna Jung, Kristen Nelson-McMillan, Nicole Shilkofski, Julianne S Perretta
OBJECTIVE: The objective was to compare resuscitation performance on simulated in-hospital cardiac arrests after traditional American Heart Association (AHA) Healthcare Provider Basic Life Support course (TradBLS) versus revised course including in-hospital skills (HospBLS). DESIGN: This study is a prospective, randomized, controlled curriculum evaluation. SETTING: Johns Hopkins Medicine Simulation Center. SUBJECTS: One hundred twenty-two first year medical students were divided into fifty-nine teams...
March 18, 2017: Resuscitation
https://www.readbyqxmd.com/read/28317442/application-of-response-surface-methodology-and-semi-mechanistic-model-to-optimize-fluoride-removal-using-crushed-concrete-in-a-fixed-bed-column
#5
Bon-Wun Gu, Chang-Gu Lee, Seong-Jik Park
The aim of this study was to investigate the removal of fluoride from aqueous solutions by using crushed concrete fines (CCF) as a filter medium. Column experiments were performed in step injection mode under varying conditions of influent solution including pH 3-7, flow rate of 0.3-0.7 mL/min, and filter depth of 10-20 cm. The performance of fixed-bed columns was evaluated on the basis of the removal ratio (Re), uptake capacity (qe), degree of sorbent used (DoSU), and sorbent usage rate (SUR) obtained from breakthrough curves (BTC)...
March 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28298038/surgical-removal-of-embolic-material-after-its-unexpected-migration-through-extracranial-intracranial-anastomosis-in-the-treatment-of-barrow-type-d-carotid-cavernous-fistula-case-report
#6
Jae-Sang Oh, Dong-Sung Kim, Jai-Joon Shim, Seok-Mann Yoon
Endovascular occlusion via the transvenous route is the favored treatment for indirect carotid-cavernous fistulas (CCFs). However, transarterial embolization can be used as an alternative method in patients with an inaccessible venous route. The authors present the case of a 49-year-old woman with a 2-month history of chemosis and proptosis in her right eye. Angiography demonstrated a Barrow Type D CCF. Transarterial Onyx embolization through the accessory meningeal artery was performed after an unsuccessful transvenous approach...
March 3, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28274780/analysis-of-heart-rate-and-oxygen-uptake-kinetics-studied-by-two-different-pseudo-random-binary-sequence-work-rate-amplitudes
#7
U Drescher, J Koschate, T Schiffer, S Schneider, U Hoffmann
PURPOSE: The aim of the study was to compare the kinetics responses of heart rate (HR), pulmonary (V˙O2pulm) and predicted muscular (V˙O2musc) oxygen uptake between two different pseudo-random binary sequence (PRBS) work rate (WR) amplitudes both below anaerobic threshold. METHODS: Eight healthy individuals performed two PRBS WR protocols implying changes between 30W and 80W and between 30W and 110W. HR and V˙O2pulm were measured beat-to-beat and breath-by-breath, respectively...
June 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28274168/resting-state-effective-connectivity-allows-auditory-hallucination%C3%A2-discrimination
#8
Manuel Graña, Leire Ozaeta, Darya Chyzhyk
Hallucinations are elusive phenomena that have been associated with psychotic behavior, but that have a high prevalence in healthy population. Some generative mechanisms of Auditory Hallucinations (AH) have been proposed in the literature, but so far empirical evidence is scarce. The most widely accepted generative mechanism hypothesis nowadays consists in the faulty workings of a network of brain areas including the emotional control, the audio and language processing, and the inhibition and self-attribution of the signals in the auditive cortex...
February 1, 2017: International Journal of Neural Systems
https://www.readbyqxmd.com/read/28248197/calibration-of-the-sup-125-sup-i-induced-x-ray-fluorescence-spectrometry-based-system-of-i-in-vivo-i-bone-strontium-determinations-using-hydroxyapatite-as-a-phantom-material-a-simulation-study
#9
Eric Da Silva, Ana Pejovic-Milic
The calibration of <i>in vivo</i> X-ray fluorescence systems of bone strontium quantification, based on <sup>125</sup>I excitation, is dependent on a coherent normalization procedure. Application of this procedure with the use of plaster of Paris (poP) as a phantom material requires the application of a coherent conversion factor (CCF) to make the calibration functions transferable between the phantom material and human bone. In this work we evaluate, with the use of Monte Carlo simulation, the potential benefit of employing a newly developed hydroxyapatite phantom material into the calibration protocol...
March 1, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28242210/cpr-quality-during-out-of-hospital-cardiac-arrest-transport
#10
Sheldon Cheskes, Adam Byers, Cathy Zhan, P Richard Verbeek, Dennis Ko, Ian R Drennan, Jason E Buick, Steven C Brooks, Steve Lin, Ahmed Taher, Laurie J Morrison
BACKGROUND: Previous studies have demonstrated significant associations between cardiopulmonary resuscitation (CPR) quality metrics and survival to hospital discharge. No adequately powered study has explored the relationship between location of resuscitation (scene vs. transport) and CPR quality. METHODS: We analyzed CPR quality data from treated adult OHCA occurring over a 40 month period beginning January 1, 2013 from the Rescu Epistry-cardiac arrest database...
May 2017: Resuscitation
https://www.readbyqxmd.com/read/28238861/comparative-analysis-of-immunocompetence-between-females-and-males-in-the-sea-cucumber-apostichopus-japonicus
#11
Jingwei Jiang, Zunchun Zhou, Ying Dong, Shan Gao, Hongjuan Sun, Zhong Chen, Aifu Yang, Hesheng Su
In order to preliminarily understand the immune difference between females and males in the sea cucumber Apostichopus japonicus, the activities assay of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO), acid phosphatase (ACP) and alkaline phosphatase (ALP) with biochemical methods, the detection of PO isozymes with native-PAGE and catechol staining, and the test of antibacterial activities with bacterial growth curve determination method were performed in this study using cell-free coelomic fluid (CCF) and coelomocyte lysate supernatant (CLS) from females and males as the samples...
February 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28220238/application-of-calcium-phosphate-flocculation-in-high-density-cell-culture-fluid-with-high-product-titer-of-monoclonal-antibody
#12
Guoqiang Chen, Zhiguo Su, Feng Li, Hui Fang Liu
The calcium phosphate [Ca3(PO4)2] precipitation was used for improving the clarification efficiency in harvest process of the monoclonal antibody (mAb) containing cell culture fluid (CCF) with high turbidity and product titer. The flocculation conditions (concentration, addition order of flocculants, pH, and operation time), and the effect of flocculants on the mAb physical chemical properties (such as distribution of charge variants and aggregates) and process-related impurities removal (such as DNA and CHOP) were evaluated in this study...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28212264/correlated-emg-oscillations-between-antagonists-during-cocontraction-in-men
#13
Yasuhide Yoshitake, Hiroaki Kanehisa, Minoru Shinohara
PURPOSE: The purpose of this study was to determine the modulation of common low-frequency oscillations in pools of motor units across antagonistic muscles because of the difference in the activation level of pools of spinal motor neurons and the presence of neuromuscular fatigue during intended cocontraction. METHODS: Ten healthy young men (21.8 ± 1.5 yr) performed intended steady cocontractions of elbow flexors and extensors at maximal and a submaximal (10% of maximal EMG) effort...
March 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28210411/carotid-cavernous-fistula-as-a-complication-of-panfacial-fracture-case-report-11-years-after-the-surgery
#14
Sylvio Luiz Costa de Moraes, Alexandre Maurity de Paula Afonso, Roberto Gomes Dos Santos, Ricardo Pereira Mattos, Bruno Gomes Duarte
The carotid-cavernous fistula (CCF) is a rare complication in patients victimized by craniofacial trauma. It involves multidisciplinary medical action. Owing to its potential complications, it is essential that maxillofacial surgery and neurosurgery specialists diagnose this condition so that appropriate treatment can be performed. The authors present a report of a case 11 years after the surgery.
March 2017: Craniomaxillofacial Trauma & Reconstruction
https://www.readbyqxmd.com/read/28193172/comparison-of-pathogenic-and-non-pathogenic-enterococcus-cecorum-strains-from-different-animal-species
#15
Arne Jung, Martin Metzner, Martin Ryll
BACKGROUND: Enterococcus cecorum (EC) infection currently is one of the most important bacterial diseases of modern broiler chickens but can also affect ducks or other avian species. However, little is known concerning pathogenesis of EC and most studies concentrate on examinations of EC strains from broilers only. The objective of this study was to compare pathogenic and commensal EC strains from different animal species concerning different phenotypic and genotypic traits. RESULTS: Pathogenic and commensal EC strains were not clearly separated from each other in a phylogenetic tree based on partial sequences of the 16S-rRNA-gene and also based on the fatty acid profile determined with gas chromatography...
February 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28191380/transorbital-approach-for-endovascular-occlusion-of-carotid-cavernous-fistulas-technical-note-and-review-of-the-literature
#16
Ching-Jen Chen, Panagiotis Mastorakos, James P Caruso, Dale Ding, Paul J Schmitt, Thomas J Buell, Daniel M Raper, Avery Evans, Steven A Newman, Mary E Jensen
Carotid-cavernous fistulas (CCFs) pose an anatomically and physiologically challenging problem for clinicians. The most common method of treatment for these lesions is transvenous endovascular embolization via the inferior petrosal sinus or the facial vein. When transvenous access is not possible, an alternate approach must be devised. We describe a case example with bilateral Barrow Type B CCFs, which were inaccessible using the traditional transvenous approach. Hence, a direct transorbital approach, performed under fluoroscopic guidance, was employed to successfully obliterate the CCF...
January 12, 2017: Curēus
https://www.readbyqxmd.com/read/28154880/accurate-quantitation-of-circulating-cell-free-mitochondrial-dna-in-plasma-by-droplet-digital-pcr
#17
Wei Ye, Xiaojun Tang, Chu Liu, Chaowei Wen, Wei Li, Jianxin Lyu
To establish a method for accurate quantitation of circulating cell-free mitochondrial DNA (ccf-mtDNA) in plasma by droplet digital PCR (ddPCR), we designed a ddPCR method to determine the copy number of ccf-mtDNA by amplifying mitochondrial ND1 (MT-ND1). To evaluate the sensitivity and specificity of the method, a recombinant pMD18-T plasmid containing MT-ND1 sequences and mtDNA-deleted (ρ(0)) HeLa cells were used, respectively. Subsequently, different plasma samples were prepared for ddPCR to evaluate the feasibility of detecting plasma ccf-mtDNA...
February 2, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28152895/effects-of-admission-adm-source-time-and-provider-on-inpatient-inpt-oncology-onc-outcomes-at-the-cleveland-clinic-foundation-ccf
#18
Lindsey Martin Goodman, Lisa A Rybicki, Alberto J Montero, Bassam N Estfan, Carolyn Best, James Stevenson
140 Background: The quality of care transfers is known to influence clinical outcomes. In an inpt onc setting at a major tertiary care referral center, patient (pt) adm originate from many different areas and times. A detailed evaluation of onc adm by source of transfer, admission time, and provider type, may identify opportunities to improve inpt clinical outcomes. METHODS: We retrospectively reviewed all adm to the inpt solid tumor onc service from July - December 2014 from CCF regional hospital emergency departments (ED), outside hospital (OSH) ED, OSH inpt services, and CCF outpt clinics...
March 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28130167/congenital-cervical-fusion-as-a-risk-factor-for-development-of-degenerative-cervical-myelopathy
#19
Aria Nouri, Allan R Martin, Stefan F Lange, Mark R N Kotter, David J Mikulis, Michael G Fehlings
BACKGROUND: Congenital fusion of cervical vertebrae, including Klippel-Feil syndrome (KFS), is a suspected risk factor for development of degenerative cervical myelopathy (DCM). We aimed to establish prevalence and degenerative patterns of congenital cervical fusion (CCF) among a global cohort of patients with DCM. METHODS: Data from 3 prospective DCM studies were merged, including clinical data for 813 patients and imaging for 592 patients. CCF was diagnosed by presence of fused cervical vertebrae without signs of degenerative fusion...
April 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28111701/thrombosis-of-venous-outflows-of-the-cavernous-sinus-possible-aetiology-of-the-cortical-venous-reflux-in-case-of-indirect-carotid-cavernous-fistulas
#20
Thomas Robert, Philippe Sylvestre, Raphaël Blanc, Daniele Botta, Gabriele Ciccio, Stanislas Smajda, Hocine Redjem, Michel Piotin
BACKGROUND: The presence of a cortical venous reflux (CVR) in a carotid-cavernous fistula (CCF) is well described and is considered to be a criterion for urgent treatment. This reflux is often associated with direct/traumatic CCF and the high-flow of the fistula alone explains the reflux. For indirect CCF, the pathophysiology of a CVR is unclear. METHODS: All patients treated endovascularly for an indirect CCF with a cortical venous reflux between 2003 and 2015 were included...
May 2017: Acta Neurochirurgica
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