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Microrna and h9c2

X R He, J C Huang, G P Chen, Q C Lin
Objective: To investigate the regulation and possible mechanism of microRNA (miR)-1249 on myocardial apoptosis in chronic intermittent hypoxia rats. Methods: A total of 16male SD rats aged 8 weeks were randomly divided into 2 groups by the random number table: normoxia control group and chronic intermittent hypoxia group (CIH) ( n =8 each). The CIH group was exposed to intermittent hypoxia every day from 9: 00 to 17: 00 for 8 consecutive weeks, while the control group received the same frequency of pulse air...
September 25, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
J Dong, Y Zhao, X-K He
OBJECTIVE: DJ-1-phosphate and tension homology deleted on chromosome ten/phosphatidylinositol-3-kinase/protein kinase B (PTEN/PI3K/AKT) signaling pathway plays a role in the regulation of ischemic reperfusion (I-R) injury. Bioinformatics analysis demonstrated that there is a complementary binding site between microRNA-192 (miR-192) and the 3'-UTR of DJ-1 mRNA. This study investigated the role of miR-192 in regulating DJ-1-PTEN/PI3K/AKT signaling pathway and myocardial I-R injury. MATERIALS AND METHODS: miR-122 and DJ-1 mRNA expressions in myocardial tissue were detected by Real-time PCR (RT-PCR)...
September 2018: European Review for Medical and Pharmacological Sciences
Zhongyi Tong, Yuting Tang, Bimei Jiang, Yanyang Wu, Yanjuan Liu, Yuanbin Li, Xianzhong Xiao
Nucleolin is a multifunctional phosphoprotein and is involved in protecting from myocardial ischemia/reperfusion (I/R) injury. The function of nucleolin is regulated by posttranslational modifications, including phosphorylation and glycosylation. To study whether phosphorylation of nucleolin (P-nucleolin) was involved in the protection from myocardial I/R injury. We investigated the expression pattern of P-nucleolin (Thr-76 and 84) in hearts subjected to I/R injury, or rat cardiac myoblast cells (H9C2) subjected to hydrogen peroxide (H 2 O 2 )...
September 7, 2018: Journal of Cellular Physiology
Xiaotong Wang, Chunyan Yang, Xueyan Liu, Ping Yang
BACKGROUND Ghrelin is a novel peptide with abundant cardioprotective effects. The miR-208 family, consisting of cardiac-specifically expressed microRNAs, are not only involved in hypertrophy and fibrosis, but are also closely related with myocyte apoptosis. This study explored the role of the miR-208 family in the protective effect of ghrelin on angiotensin II (Ang II)-induced apoptosis. MATERIAL AND METHODS H9c2 cells were exposed to Ang II with or without ghrelin. Cell viability was detected by MTT assay and the percentage of apoptotic cells was confirmed by flow cytometry...
September 23, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Rui An, Jianxin Feng, Cong Xi, Jian Xu, Lijun Sun
Myocardial dysfunction is a major manifestation of sepsis and closely associated with the increased mortality. MicroRNA-146 is one of the most important microRNAs identified as a potent negative regulator in innate immune and inflammatory responses induced by lipopolysaccharide (LPS). We aimed to identify the role and potential regulatory mechanism of miR-146a in sepsis-induced cardiac dysfunction with the induction of ErbB4 signaling. H9C2 cells were treated with LPS to induce sepsis, and miR-146a overexpression significantly increased the cell viability, reduced the apoptosis and ROS level, and attenuated the release of proinflammatory cytokines including TNF- α and IL-1 β ...
2018: Oxidative Medicine and Cellular Longevity
Lu Gao, Xiaofang Wang, Sen Guo, Lili Xiao, Cui Liang, Zheng Wang, Yapeng Li, Yuzhou Liu, Rui Yao, Yuan Liu, Yanzhou Zhang
The HOX transcript antisense RNA (HOTAIR) long noncoding RNA (lncRNA), a highly abundant and conserved imprinted gene, has been implicated in many essential biological processes and diseases. However, to date, the significance of HOTAIR in diabetic cardiomyopathy (DCM) has never been investigated. The current study was designed to determine whether DCM can be regulated by HOTAIR and to elucidate the related mechanism. In vivo, streptozotocin (STZ) was injected intraperitoneally to induce type 1 diabetes in mice...
September 14, 2018: Journal of Cellular Physiology
Yongxia Cheng, Wei Zhao, Xiaodong Zhang, Lixin Sun, Heran Yang, Ying Wang, Yong Cao, Yanhui Chu, Guibo Liu
Diabetic cardiomyopathy (DCM) is characterized by abnormal myocardial structure or performance. It has been suggested that microRNA-1 (miR-1) may be abnormally expressed in the hearts of patients with diabetes. In the present study, the role of miR-1 in glucose-induced apoptosis and its underlying mechanism of action was investigated in rat cardiomyocyte H9C2 cells. Cells were transfected with anti-miR-1 or miR-1-overexpression plasmids and the expression of miR-1 and liver X receptor α (LXRα) were determined by reverse transcription-quantitative polymerase chain reaction analysis...
September 2018: Experimental and Therapeutic Medicine
Zhi-Qiang Tian, Hong Jiang, Zhi-Bing Lu
Background: MicroRNAs play important roles in regulation of the cardiovascular system. The purpose of this study was to investigate microRNA-320 (miR-320) expression in myocardial ischemia-reperfusion (I/R) injury and the roles of miR-320 in cardiomyocyte apoptosis by targeting AKIP1 (A kinase interacting protein 1). Methods: The level of miR-320 was detected using quantitative real-time polymerase chain reaction (qRT-PCR), and cardiomyocyte apoptosis was detected via terminal dUTP nick end-labeling assay...
2018: Cellular & Molecular Biology Letters
Xinling Zhang, Chunxiang Zhang, Nan Wang, Yan Li, Debing Zhang, Qiang Li
BACKGROUND/AIMS: Acute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODS: H9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC)...
2018: Cellular Physiology and Biochemistry
Yimin Xiao, Yanxia Zhang, Yueqiu Chen, Jingjing Li, Zihan Zhang, Yimin Sun, Han Shen, Zhenao Zhao, Zan Huang, Wencheng Zhang, Weiqian Chen, Zhenya Shen
Follistatin-like 1 (Fstl1) protects cardiomyocytes from a broad spectrum of pathologic injuries including myocardial infarction (MI). It is worthy of note that although cardiac Fstl1 is elevated in post-MI microenvironment, its cardioprotective role is still restricted to a limited extent considering the frequency and severity of adverse cardiac remodeling following MI. We therefore propose that intrinsic Fstl1-suppressing microRNA (miRNA) may exist in the heart and its neutralization may further facilitate post-MI recovery...
August 13, 2018: Human Gene Therapy
Jie Ren, Wei Liu, Guang-Cai Li, Meng Jin, Zhen-Xi You, Hui-Guo Liu, Yi Hu
Atorvastatin is proven to ameliorate cardiac hypertrophy induced by chronic intermittent hypoxia (CIH). However, little is known about the mechanism by which atorvastatin modulates CIH-induced cardiac hypertrophy, and whether specific hypertrophyrelated microRNAs are involved in the modulation. MiR-31 plays key roles in the development of cardiac hypertrophy induced by ischemia/hypoxia. This study examined whether miR-31 was involved in the protective role of atorvastatin against CIH-induced myocardial hypertrophy...
June 2018: Current medical science
Qijing Wang, Yue Zhang, Fang Le, Ning Wang, Fan Zhang, Yuqin Luo, Yiyun Lou, Minhao Hu, Liya Wang, Lisa M Thurston, Xiangrong Xu, Fan Jin
Epidemiological studies have revealed that offspring conceived by in vitro fertilization (IVF) have an elevated risk of cardiovascular malformations at birth, and are more predisposed to cardiovascular diseases in late childhood. Unfortunately, the underlying mechanisms influencing compromised cardiovascular function in IVF-conceived children remain unclear. Moreover, the continuing implications for the cardiovascular health of these children as they move into adulthood and old age, have yet to be determined...
July 13, 2018: Biology of Reproduction
Grasieli de Oliveira, Paula Paccielli Freire, Ana Carolina Mieko Omoto, Sarah Santiloni Cury, Cesar Seigi Fuziwara, Edna Teruko Kimura, Maeli Dal-Pai-Silva, Robson Francisco Carvalho
Several studies have demonstrated dysregulated cardiac microRNAs (miRNAs) following cardiac stress and development of cardiac hypertrophy and failure. miRNAs are also differentially expressed in the inflammation that occurs in heart failure and, among these inflammatory-related miRNAs, the miR-155 has been implicated in the regulation of cardiac hypertrophy. Despite these data showing the role of miRNA-155 in cardiomyocyte hypertrophy under a hypertrophic stimulus, it is also important to understand the endogenous regulation of this miRNA without a hypertrophic stimulus to fully appreciate its function in this cell type...
November 15, 2018: Gene
Oliver A Kent, Charles Steenbergen, Samarjit Das
MicroRNA (miRNA) is small non-coding RNA which inhibits post-transcriptional messenger RNA (mRNA) expression. Human diseases, such as cancer and cardiovascular disease, have been shown to activate tissue and/or cell-specific miRNA expression associated with disease progression. The inhibition of miRNA expression offers the potential for a therapeutic intervention. However, traditional approaches to inhibit miRNAs, employing antagomir oligonucleotides, affect specific miRNA functions upon global delivery. Herein, we present a protocol for the in vivo cardio-specific inhibition of the miR-181 family in a rat model...
June 15, 2018: Journal of Visualized Experiments: JoVE
Hui Pan, Linlin Zhu
BACKGROUND: The cardioprotective role of Angelica sinensis has been proven in previous studies. However, the effects of Angelica sinensis polysaccharide (ASP, major bioactive component of Angelica sinensis) on myocardial infarction (MI) remain unclear. This study aimed to investigate the effects of ASP on hypoxia-induced H9c2 cell injury as well as the underlying mechanisms. METHODS: We constructed in vitro hypoxic model to mimic MI. Cell viability, proliferation and apoptosis were respectively measured by using CCK-8 assay, Western blot analysis, and flow cytometry assay/Western blot analysis, to evaluate cell injury after treatments...
October 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jideng Ma, Jinwei Zhang, Yujie Wang, Keren Long, Xun Wang, Long Jin, Qianzi Tang, Li Zhu, Guoqing Tang, Xuewei Li, Mingzhou Li
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs involved in regulating various biological processes at the post-transcription level. Accumulating evidence suggests that hypoxia caused by acute myocardial infarction induces cardiomyocyte damage including apoptosis. Previous studies regarding the miRNAome in H9c2 cells under hypoxia have shown that hypoxia modulates miRNA expression in H9c2 cells, including miR-532-5p. We therefore investigated whether miR-532-5p has a potential function in the cardiomyocyte response to hypoxia...
October 30, 2018: Gene
Xuan Zhang, Long Cheng, Longhe Xu, Yunliang Zhang, Yitian Yang, Qiang Fu, Weidong Mi, Hao Li
BACKGROUND: Ischemic postconditioning (I/Post) is an endogenous protection mechanism that reduces injury induced by ischemia-reperfusion (I/R). It remains controversial whether I/Post protects against I/R injury to the aging heart. The long non-coding RNA, H19 protects H9c2 cells against hypoxia-induced injury. This study aimed to elucidate the role of H19 in the hypoxic postconditioning (H/Post) of aged cardiomyocytes. METHODS: Senescence induced by D-galactose in primary cardiomyocytes from neonatal Sprague-Dawley rats was measured by senescence-associated β-galactosidase staining...
June 27, 2018: Shock
Wenzheng Xia, Meng Hou
Doxorubicin (DOXO) is a chemotherapeutic agent widely used in the treatment of various types of cancer. However, cardiotoxicity is a major side effect of DOXO therapy due to the ability of this compound to induce cardiac cellular senescence. It is well known that microRNA (miR)-34a serves a role in cardiac dysfunction and ageing, and that it is involved in several cellular processes associated with DOXO-induced cardiotoxicity. Furthermore, mesenchymal stem cell (MSC)-based therapies have been reported to modulate cellular senescence...
June 2018: Oncology Letters
Bing Xing, Qiao-Ju Li, Hu Li, Sha-Sha Chen, Zhi-Yuan Cui, Jie Ma, Zai-Wei Zhang
Myocardial infarction (MI) is an important cause of cardiovascular disease. microRNAs (miRNAs) have been indicated as pivotal regulators in the physiological and pathological processes of heart diseases. The purpose of this study was to investigate the role of miR-140-5p in hypoxia-induced cell injury in H9c2 cells and its underlying mechanism. H9c2 cells were subjected to hypoxia, before which the expression levels of miR-140-5p and MLK3 were overexpressed or knocked down through transient transfection. The efficiency of transfection was verified by qRT-PCR and Western blotting...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hongwei Zhao, Xiaobei Zhang, Ying Zheng, Yuan Li, Xiaokun Wang, Nan Hu, Peng Zhou, Kaiyuan Wang
We aimed to evaluate the cardioprotective effect and mechanism of propofol in anthracycline-induced cardiomyocyte apoptosis. We selected the rat myocardial cell line, H9c2, and primary cardiomyocytes for in vitro study. The cardiomyocytes were treated with vehicle, Adriamycin® (ADM), propofol, or a combination of ADM and propofol. The proportion of apoptotic cells and the expression of miR-181a were detected by flow cytometry and real-time PCR, respectively. Luciferase assays were performed to explore the direct target gene of miR-181a...
2018: Oxidative Medicine and Cellular Longevity
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