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Microrna and h9c2

Yimin Xiao, Yanxia Zhang, Yueqiu Chen, Jingjing Li, Zihan Zhang, Yimin Sun, Han Shen, Zhenao Zhao, Zan Huang, Wencheng Zhang, Weiqian Chen, Zhenya Shen
Follistatin-like 1 (Fstl1) protects cardiomyocytes from a broad spectrum of pathologic injuries including myocardial infarction (MI). It is worthy of note that although cardiac Fstl1 is elevated in post-MI microenvironment, its cardioprotective role is still restricted to a limited extent considering the frequency and severity of adverse cardiac remodeling following MI. We therefore propose that intrinsic Fstl1-suppressing microRNA (miRNA) may exist in the heart and its neutralization may further facilitate post-MI recovery...
August 13, 2018: Human Gene Therapy
Jie Ren, Wei Liu, Guang-Cai Li, Meng Jin, Zhen-Xi You, Hui-Guo Liu, Yi Hu
Atorvastatin is proven to ameliorate cardiac hypertrophy induced by chronic intermittent hypoxia (CIH). However, little is known about the mechanism by which atorvastatin modulates CIH-induced cardiac hypertrophy, and whether specific hypertrophyrelated microRNAs are involved in the modulation. MiR-31 plays key roles in the development of cardiac hypertrophy induced by ischemia/hypoxia. This study examined whether miR-31 was involved in the protective role of atorvastatin against CIH-induced myocardial hypertrophy...
June 2018: Current medical science
Qijing Wang, Yue Zhang, Fang Le, Ning Wang, Fan Zhang, Yuqin Luo, Yiyun Lou, Minhao Hu, Liya Wang, Lisa M Thurston, Xiangrong Xu, Fan Jin
Epidemiological studies have revealed that offspring conceived by in vitro fertilization (IVF) have an elevated risk of cardiovascular malformations at birth, and are more predisposed to cardiovascular diseases in late childhood. Unfortunately, the underlying mechanisms influencing compromised cardiovascular function in IVF-conceived children remain unclear. Moreover, the continuing implications for the cardiovascular health of these children as they move into adulthood and old age, have yet to be determined...
July 13, 2018: Biology of Reproduction
Grasieli de Oliveira, Paula Paccielli Freire, Ana Carolina Mieko Omoto, Sarah Santiloni Cury, César Seigi Fuziwara, Edna Teruko Kimura, Maeli Dal-Pai-Silva, Robson Francisco Carvalho
Several studies have demonstrated dysregulated cardiac microRNAs (miRNAs) following cardiac stress and development of cardiac hypertrophy and failure. miRNAs are also differentially expressed in the inflammation that occurs in heart failure and, among these inflammatory-related miRNAs, the miR-155 has been implicated in the regulation of cardiac hypertrophy. Despite these data showing the role of miRNA-155 in cardiomyocyte hypertrophy under a hypertrophic stimulus, it is also important to understand the endogenous regulation of this miRNA without a hypertrophic stimulus to fully appreciate its function in this cell type...
July 7, 2018: Gene
Oliver A Kent, Charles Steenbergen, Samarjit Das
MicroRNA (miRNA) is small non-coding RNA which inhibits post-transcriptional messenger RNA (mRNA) expression. Human diseases, such as cancer and cardiovascular disease, have been shown to activate tissue and/or cell-specific miRNA expression associated with disease progression. The inhibition of miRNA expression offers the potential for a therapeutic intervention. However, traditional approaches to inhibit miRNAs, employing antagomir oligonucleotides, affect specific miRNA functions upon global delivery. Herein, we present a protocol for the in vivo cardio-specific inhibition of the miR-181 family in a rat model...
June 15, 2018: Journal of Visualized Experiments: JoVE
Hui Pan, Linlin Zhu
BACKGROUND: The cardioprotective role of Angelica sinensis has been proven in previous studies. However, the effects of Angelica sinensis polysaccharide (ASP, major bioactive component of Angelica sinensis) on myocardial infarction (MI) remain unclear. This study aimed to investigate the effects of ASP on hypoxia-induced H9c2 cell injury as well as the underlying mechanisms. METHODS: We constructed in vitro hypoxic model to mimic MI. Cell viability, proliferation and apoptosis were respectively measured by using CCK-8 assay, Western blot analysis, and flow cytometry assay/Western blot analysis, to evaluate cell injury after treatments...
June 27, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jideng Ma, Jinwei Zhang, Yujie Wang, Keren Long, Xun Wang, Long Jin, Qianzi Tang, Li Zhu, Guoqing Tang, Xuewei Li, Mingzhou Li
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs involved in regulating various biological processes at the post-transcription level. Accumulating evidence suggests that hypoxia caused by acute myocardial infarction induces cardiomyocyte damage including apoptosis. Previous studies regarding the miRNAome in H9c2 cells under hypoxia have shown that hypoxia modulates miRNA expression in H9c2 cells, including miR-532-5p. We therefore investigated whether miR-532-5p has a potential function in the cardiomyocyte response to hypoxia...
June 26, 2018: Gene
Xuan Zhang, Long Cheng, Longhe Xu, Yunliang Zhang, Yitian Yang, Qiang Fu, Weidong Mi, Hao Li
BACKGROUND: Ischemic postconditioning (I/Post) is an endogenous protection mechanism that reduces injury induced by ischemia-reperfusion (I/R). It remains controversial whether I/Post protects against I/R injury to the aging heart. The long non-coding RNA, H19 protects H9c2 cells against hypoxia-induced injury. This study aimed to elucidate the role of H19 in the hypoxic postconditioning (H/Post) of aged cardiomyocytes. METHODS: Senescence induced by D-galactose in primary cardiomyocytes from neonatal Sprague-Dawley rats was measured by senescence-associated β-galactosidase staining...
June 27, 2018: Shock
Wenzheng Xia, Meng Hou
Doxorubicin (DOXO) is a chemotherapeutic agent widely used in the treatment of various types of cancer. However, cardiotoxicity is a major side effect of DOXO therapy due to the ability of this compound to induce cardiac cellular senescence. It is well known that microRNA (miR)-34a serves a role in cardiac dysfunction and ageing, and that it is involved in several cellular processes associated with DOXO-induced cardiotoxicity. Furthermore, mesenchymal stem cell (MSC)-based therapies have been reported to modulate cellular senescence...
June 2018: Oncology Letters
Bing Xing, Qiao-Ju Li, Hu Li, Sha-Sha Chen, Zhi-Yuan Cui, Jie Ma, Zai-Wei Zhang
Myocardial infarction (MI) is an important cause of cardiovascular disease. microRNAs (miRNAs) have been indicated as pivotal regulators in the physiological and pathological processes of heart diseases. The purpose of this study was to investigate the role of miR-140-5p in hypoxia-induced cell injury in H9c2 cells and its underlying mechanism. H9c2 cells were subjected to hypoxia, before which the expression levels of miR-140-5p and MLK3 were overexpressed or knocked down through transient transfection. The efficiency of transfection was verified by qRT-PCR and Western blotting...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hongwei Zhao, Xiaobei Zhang, Ying Zheng, Yuan Li, Xiaokun Wang, Nan Hu, Peng Zhou, Kaiyuan Wang
We aimed to evaluate the cardioprotective effect and mechanism of propofol in anthracycline-induced cardiomyocyte apoptosis. We selected the rat myocardial cell line, H9c2, and primary cardiomyocytes for in vitro study. The cardiomyocytes were treated with vehicle, Adriamycin® (ADM), propofol, or a combination of ADM and propofol. The proportion of apoptotic cells and the expression of miR-181a were detected by flow cytometry and real-time PCR, respectively. Luciferase assays were performed to explore the direct target gene of miR-181a...
2018: Oxidative Medicine and Cellular Longevity
Xianglin Chu, Yiqing Wang, Liewen Pang, Jiechun Huang, Xiaotian Sun, Xiaofeng Chen
Cardiac remodeling is a common pathophysiological change associated with acute myocardial infarction (AMI). Recent evidence indicates that microRNAs are strong posttranscriptional regulators which play an important role in regulating the microenvironment of myocardial tissue after AMI. In this study, we sought to explore the potential role and underlying mechanism of miR-130 in AMI. H9c2 cells were cultured under hypoxic conditions to simulate myocardial infarction. The influence of aberrantly expressed miR-130 on H9c2 cells under hypoxia was also estimated with RT-PCR, western blot and enzyme-linked immunosorbent assay...
May 15, 2018: Journal of Cellular Biochemistry
Jing Cheng, Qianfu Wu, Rong Lv, Li Huang, Banglong Xu, Xianbao Wang, Aihua Chen, Fei He
BACKGROUND/AIMS: The present study aimed to detect the expression of miR-449a and investigate the effect of miR-449a on cell injury in cardiomyocytes subjected to hypoxia/ reoxygenation (H/R) and its underlying mechanisms. METHODS: The expression of miR-449a was determined using reverse transcription-polymerase chain reaction in both neonatal rat ventricular myocytes and H9C2 cells. For gain-of-function and loss-of-function studies, H9C2 cells were transfected with either miR-449a mimics or miR-449a inhibitor...
2018: Cellular Physiology and Biochemistry
Yuki Tatekoshi, Masaya Tanno, Hidemichi Kouzu, Koki Abe, Takayuki Miki, Atsushi Kuno, Toshiyuki Yano, Satoko Ishikawa, Wataru Ohwada, Tatsuya Sato, Takeshi Niinuma, Hiromu Suzuki, Tetsuji Miura
AMP deaminase (AMPD) plays a crucial role in adenine nucleotide metabolism. Recently we found that upregulated AMPD activity is associated with ATP depletion and contractile dysfunction under the condition of pressure overloading in the heart of a rat model of type 2 diabetes mellitus (T2DM), OLETF. Here we examined the mechanism of AMPD upregulation by T2DM. The protein level of 90-kDa full-length AMPD3 was increased in whole myocardial lysates by 55% in OLETF compared to those in LETO, a non-diabetic control...
June 2018: Journal of Molecular and Cellular Cardiology
Min Feng, Zongqing Li, Dong Wang, Fang Wang, Chenyan Wang, Chunfang Wang, Faming Ding
BACKGROUND: Myocardial infarction (MI), a leading cause of mortality, is identified as the myocardial necrosis due to prolonged ischemia. Hypoxia, resulting from ischemia, induces cell apoptosis during MI. Since miR-210 is a hypoxia inducible factor, we aimed to explore the functional role of miR-210 in hypoxic H9c2 cells. METHODS: Hypoxia-induced cell injury was evaluated according to cell viability, apoptosis and expression of apoptosis-associated proteins. miR-210 expression after hypoxia was tested...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Mengjie Yan, Sibao Yang, Fanbo Meng, Zhihui Zhao, Zhisen Tian, Ping Yang
MicroRNAs participate in a variety of physiological and pathophysiological processes in various organs including the heart. Our previous work revealed that the level of miR-199a-5p was significantly higher in failing hearts than in control hearts. However, whether it is associated with the progression of heart failure (HF) and mediates cardiomyocyte apoptosis remained unclear. In the present study, we used various biochemical and molecular biological approaches to investigate the changes in miR-199a-5p levels in failing hearts in a rat model induced by acute myocardial infarction...
April 27, 2018: Scientific Reports
Qingfeng Meng, Yang Liu, Xiuyue Huo, Hui Sun, Yingcui Wang, Fangfang Bu
Myocardial ischemia‑reperfusion (I/R) injury is a major cause of cardiovascular disease worldwide, and microRNAs have been implicated in the regulation of pathological and physiological processes in myocardial I/R injury. The present study aimed to investigate the role of microRNA (miR)‑221‑3p in myocardial I/R injury. Cell death and lactate dehydrogenase (LDH) activity were increased in hydrogen peroxide (H2O2)‑treated H9c2 cells, as measured by flow cytometry and an LDH detection kit. The expression of miR‑221‑3p was elevated in H2O2‑incubated cells and in remote areas of the rat I/R model, examined using reverse transcription‑quantitative polymerase chain reaction analysis...
July 2018: International Journal of Molecular Medicine
Y Zhai, N Ding
OBJECTIVE: To investigate the expression level of microRNA-194 in myocardial injury induced by lipopolysaccharide (LPS) and its underlying mechanism. MATERIALS AND METHODS: LPS-induced H9c2 cardiomyocytes injury model was established. The expression level of microRNA-194 at different treatment time points was detected. Survival and apoptosis of cardiomyocytes were detected after overexpression or knockdown of microRNA-194. The target genes of microRNA-194 were predicted by bioinformatics analysis...
April 2018: European Review for Medical and Pharmacological Sciences
Shouwen Zhang, Rongjun Zhang, Fangfang Wu, Xinhua Li
Ischemic reperfusion (I/R) injury is a serious problem in the treatment of ischemic heart disease. MicroRNA-208a (miR-208a) is a cardiac-specific or cardiac-enriched miRNA. This study was aimed to assess the role of miR-208a in I/R injury. H9c2 cells were used to simulate I/R injury in vitro. miR-208a expression level was measured by qPCR. H9c2 cells after simulated I/R injury were transfected with miR-208a mimic, AOS-miR-208a or negative controls. LDH release, MDA and SOD contents were measured by corresponding purchased detection kits, respectively...
May 30, 2018: International Heart Journal
Hai-Jian Liu, Bin Liu
Isoflurane is one of the most frequently used volatile anesthetics in clinical practice for inhalational anesthesia. It is widely studied that isoflurane mediates cardioprotection during multiple pathological processes. However, the precise mechanisms have not been fully elucidated. Neonatal cardiomyocytes were isolated and cultured, followed by treatments with isoflurane at 0, 50, 100 or 200 µM. Rat cardiomyoblast cell line, H9c2, was treated with H2 O2 . Expression of miR-23 was measured by qRT-PCR. The cell survival rate of H9c2 in response to H2 O2 treatments was evaluated by MTT assay...
April 7, 2018: Cardiovascular Toxicology
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