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Microrna and h9c2

Liqiong Xu, Wei Chen, Min Ma, Anfang Chen, Chengyue Tang, Chengwei Zhang, Lin Cai
OBJECTIVE: Current study focused on the influence of miR-200b-3p on cardiocyte apoptosis of diabetic cardiomyopathy (DCM) by regulating CD36 and peroxisome proliferator-activated receptor γ (PPAR-γ) signaling pathway. METHODS: Bioinformatic analysis was used to analyze differentially expressed microRNA (miRNAs), messenger RNAs (mRNAs) and activated pathways in DCM. And then quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to verify expression of miR-200b-3p and CD36 in DCM model rats and glucose treated H9c2 cell line...
December 2, 2018: Journal of Cellular Biochemistry
Xiaodi Zhou, Bo Chang, Yuzhong Gu
OBJECTIVE: The aim of the study was to investigate the role of microRNA-21 (miR-21) in cardiomyocyte apoptosis and to determine a possible mechanism. METHODS: H9c2 embryonic rat heart-derived cells were used in the study. Cell viability was determined using the 3-(4.5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide (MTT) assay, and flow cytometry was used to evaluate cell apoptosis. Reverse transcription-polymerase chain reaction and western blot assays were used to detect mRNA and protein expression of the apoptosis-related proteins and miR-21...
December 2018: Anatolian Journal of Cardiology
Yang Li, Xiping Liu, Aolin Du, Xiaolong Zhu, Bo Yu
Myocarditis is an inflammatory disease of the myocardium. MicroRNA-203 (miR-203) is involved in various physiological and pathological processes. In this work, we aimed to explore the roles and potential mechanisms of miR-203 in myocarditis in vitro. Cardiomyocyte H9c2 was subjected to 10 μg/mL lipopolysaccharide (LPS) for 24 hours. Real-time polymerase chain reaction analysis revealed that LPS upregulated miR-203 expression in H9c2 cells. Cell counting kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays demonstrated that inhibition of miR-203 reduced cell injury induced by LPS...
November 28, 2018: Journal of Cellular Biochemistry
Wei-Ting Chang, Chih-Hsin Hsu, Tzu-Ling Huang, Ying-Ching Tsai, Chun-Yen Chiang, Zhih-Cherng Chen, Jhih-Yuan Shih
Background: The outcome of pulmonary hypertension (PH) mainly depends on the development of right ventricular (RV) dysfunction, and survival among patients with different etiologies of PH varies. Chronic hypoxia is a major cause of secondary PH, however the mechanisms of its associated RV dysfunction are largely unknown. Herein, we studied the role of microRNA-21 (miR-21) in hypoxia-induced RV dysfunction. Methods: In this longitudinal, prospective study, we enrolled 41 patients with hypoxia-induced PH...
November 2018: Acta Cardiologica Sinica
Liuhui Chang, Xiaoming Chai, Peiming Chen, Jianfang Cao, Hong Xie, Jiang Zhu
This study aimed to investigate the role of microRNA‑181b‑5p (miR‑181b‑5p) in starvation‑induced cardiomyocyte autophagy by targeting heat shock protein family A member 5 (Hspa5). For this purpose, H9c2 cardiomyocytes and neonatal rat ventricular myocytes (NRVMs) were glucose‑starved in Earle's Balanced Salt Solution (EBSS) for different periods of time (0, 2, 4, 6 and 8 h). RT‑qPCR analysis was performed to examine the expression of miR‑181b‑5p in the different groups. Immunofluorescence was performed to detect the expression of LC3...
January 2019: International Journal of Molecular Medicine
Chang-Lin Zhai, Guan-Min Tang, Gang Qian, Hui-Lin Hu, Shi-Jun Wang, Dong Yin, Song Zhang
Cardiovascular ischemic disease is a large class of diseases that are harmful to human health. The significant role of microRNAs (miRNAs) in terms of controlling cardiac injury has been reported in latest studies. MiR-98 is very important in regulating the apoptosis, the differentiation, the growth as well as the metastasis of cells. Nevertheless, the effect of miR-98 in the cardiac ischemia reperfusion (I/R) injury has rarely been investigated. In the current research, we found that the miR-98 expression was down-regulated in the cardiomyocytes subjected to hypoxia/reoxygenation (H/R) and in the myocardium of the I/R rats...
November 12, 2018: IUBMB Life
Weijun Zhu, Junrong Lei, Xiaojun Bai, Rui Wang, Yulan Ye, Jianjun Bao
Coronary heart disease is the second highest specific cause of death. H9c2 cardiomyocytes were subjected to hypoxia (1% O2 ) for 0, 6, 12, 24 and 48 h. Cell apoptosis and the activity of caspase3/7 was detected using ELISA; western blot was applied to determine the cleaved-caspase3 (c-caspase3), cleaved-PARP (c-PARP) and cytochrome C (Cyto C) expression after the inhibitor negative control (in-NC), miR-503 inhibitor, mimic negative control (mi-NC) and miR-503 mimic were transfected into cells for 48 h. Moreover, flow cytometry was applied to evaluate mitochondrial membrane potential...
November 4, 2018: Biochemical and Biophysical Research Communications
Z-X Guo, F-Z Zhou, W Song, L-L Yu, W-J Yan, L-H Yin, H Sang, H-Y Zhang
OBJECTIVE: MicroRNAs (miRNAs) are small single-stranded RNAs in eukaryotic cells, which play important regulatory roles in the pathogenesis of various diseases. We aimed to investigate the effects of miRNA-101 (miR-101) on hypoxia-induced myocardial infarction (MI) cell injury model (myocardial H9c2 cell injury model). The possible target gene of miR-101 was also analyzed. MATERIALS AND METHODS: H9c2 cells were exposed to hypoxia treatment. Cell viability, migration, invasion, apoptosis and the expression of miR-101 were detected using CCK-8 assay, transwell assay, flow cytometer analysis, Western blotting and qRT-PCR, respectively...
October 2018: European Review for Medical and Pharmacological Sciences
Qingxiong Yue, Cuiting Zhao, Yonghuai Wang, Lanting Zhao, Qing Zhu, Guangyuan Li, Nan Wu, Dalin Jia, Chunyan Ma
Palmitic acid (PA) can induce lipotoxic damage to cardiomyocytes, although its precise mechanism of action has not been completely elucidated. Growth arrest‑specific transcript 5 (GAS5) is a long noncoding RNA that serves a regulatory role in several pathological processes, including tumorigenesis, stroke, cardiac fibrosis and osteoarthritis; however, its role in PA‑induced myocardial injury remains elusive. The present study aimed to explore the role and underlying mechanism of GAS5 on PA‑induced myocardial injury...
December 2018: Molecular Medicine Reports
Junjie Pan, Maimaitijiang Alimujiang, Qiying Chen, Haiming Shi, Xinping Luo
Multiple studies demonstrated that early growth response factor 1 (EGR1) induces myocardial damage after acute myocardial infarction (AMI). Recent evidence indicates that microRNAs (miRNA) play an important role in exosome-mediated cardioprotection after AMI. Bioinformatics analysis has shown that miR-146a can regulate the expression of EGR1, so the aim of this study was to determine if miR-146a plays a role in exosome-mediated cardioprotection by regulation of EGR1 after AMI. Exosomes were isolated from wild- or miR-146a-modified adipose-derived stem cells (ADSCs), and the therapeutic effect of exosomes was assessed in an AMI model in rats and hypoxic-induced H9c2 model cells...
October 26, 2018: Journal of Cellular Biochemistry
Hongbin Zhang, Xiaoqun Zhang, Jun Zhang
Ischemic heart disease (IHD) is a significant cause of cardiovascular diseases. MicroRNAs (miRNAs) have been thought to be critical regulators in the heart diseases. The present study was aimed to investigate the effect of miR-129-5p on the autophagy and apoptosis by targeting ATG14 as well as how miR-129-5p worked through the PI3K/AKT/mTOR signaling pathway in H2 O2 -induced H9c2 cells. H9c2 cells were induced by H2 O2 , after which the expression of miR-129-5p was decreased. Reverse transcription-quantitative polymerase chain reaction (qRT-PCR) was performed to detect the expression level of miR-129-5p in H9c2 cells...
October 19, 2018: Biochemical and Biophysical Research Communications
X R He, J C Huang, G P Chen, Q C Lin
Objective: To investigate the regulation and possible mechanism of microRNA (miR)-1249 on myocardial apoptosis in chronic intermittent hypoxia rats. Methods: A total of 16male SD rats aged 8 weeks were randomly divided into 2 groups by the random number table: normoxia control group and chronic intermittent hypoxia group (CIH) ( n =8 each). The CIH group was exposed to intermittent hypoxia every day from 9: 00 to 17: 00 for 8 consecutive weeks, while the control group received the same frequency of pulse air...
September 25, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
J Dong, Y Zhao, X-K He
OBJECTIVE: DJ-1-phosphate and tension homology deleted on chromosome ten/phosphatidylinositol-3-kinase/protein kinase B (PTEN/PI3K/AKT) signaling pathway plays a role in the regulation of ischemic reperfusion (I-R) injury. Bioinformatics analysis demonstrated that there is a complementary binding site between microRNA-192 (miR-192) and the 3'-UTR of DJ-1 mRNA. This study investigated the role of miR-192 in regulating DJ-1-PTEN/PI3K/AKT signaling pathway and myocardial I-R injury. MATERIALS AND METHODS: miR-122 and DJ-1 mRNA expressions in myocardial tissue were detected by Real-time PCR (RT-PCR)...
September 2018: European Review for Medical and Pharmacological Sciences
Zhongyi Tong, Yuting Tang, Bimei Jiang, Yanyang Wu, Yanjuan Liu, Yuanbin Li, Xianzhong Xiao
Nucleolin is a multifunctional phosphoprotein and is involved in protecting from myocardial ischemia/reperfusion (I/R) injury. The function of nucleolin is regulated by posttranslational modifications, including phosphorylation and glycosylation. To study whether phosphorylation of nucleolin (P-nucleolin) was involved in the protection from myocardial I/R injury. We investigated the expression pattern of P-nucleolin (Thr-76 and 84) in hearts subjected to I/R injury, or rat cardiac myoblast cells (H9C2) subjected to hydrogen peroxide (H 2 O 2 )...
September 7, 2018: Journal of Cellular Physiology
Xiaotong Wang, Chunyan Yang, Xueyan Liu, Ping Yang
BACKGROUND Ghrelin is a novel peptide with abundant cardioprotective effects. The miR-208 family, consisting of cardiac-specifically expressed microRNAs, are not only involved in hypertrophy and fibrosis, but are also closely related with myocyte apoptosis. This study explored the role of the miR-208 family in the protective effect of ghrelin on angiotensin II (Ang II)-induced apoptosis. MATERIAL AND METHODS H9c2 cells were exposed to Ang II with or without ghrelin. Cell viability was detected by MTT assay and the percentage of apoptotic cells was confirmed by flow cytometry...
September 23, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Rui An, Jianxin Feng, Cong Xi, Jian Xu, Lijun Sun
Myocardial dysfunction is a major manifestation of sepsis and closely associated with the increased mortality. MicroRNA-146 is one of the most important microRNAs identified as a potent negative regulator in innate immune and inflammatory responses induced by lipopolysaccharide (LPS). We aimed to identify the role and potential regulatory mechanism of miR-146a in sepsis-induced cardiac dysfunction with the induction of ErbB4 signaling. H9C2 cells were treated with LPS to induce sepsis, and miR-146a overexpression significantly increased the cell viability, reduced the apoptosis and ROS level, and attenuated the release of proinflammatory cytokines including TNF- α and IL-1 β ...
2018: Oxidative Medicine and Cellular Longevity
Lu Gao, Xiaofang Wang, Sen Guo, Lili Xiao, Cui Liang, Zheng Wang, Yapeng Li, Yuzhou Liu, Rui Yao, Yuan Liu, Yanzhou Zhang
The HOX transcript antisense RNA (HOTAIR) long noncoding RNA (lncRNA), a highly abundant and conserved imprinted gene, has been implicated in many essential biological processes and diseases. However, to date, the significance of HOTAIR in diabetic cardiomyopathy (DCM) has never been investigated. The current study was designed to determine whether DCM can be regulated by HOTAIR and to elucidate the related mechanism. In vivo, streptozotocin (STZ) was injected intraperitoneally to induce type 1 diabetes in mice...
September 14, 2018: Journal of Cellular Physiology
Yongxia Cheng, Wei Zhao, Xiaodong Zhang, Lixin Sun, Heran Yang, Ying Wang, Yong Cao, Yanhui Chu, Guibo Liu
Diabetic cardiomyopathy (DCM) is characterized by abnormal myocardial structure or performance. It has been suggested that microRNA-1 (miR-1) may be abnormally expressed in the hearts of patients with diabetes. In the present study, the role of miR-1 in glucose-induced apoptosis and its underlying mechanism of action was investigated in rat cardiomyocyte H9C2 cells. Cells were transfected with anti-miR-1 or miR-1-overexpression plasmids and the expression of miR-1 and liver X receptor α (LXRα) were determined by reverse transcription-quantitative polymerase chain reaction analysis...
September 2018: Experimental and Therapeutic Medicine
Zhi-Qiang Tian, Hong Jiang, Zhi-Bing Lu
Background: MicroRNAs play important roles in regulation of the cardiovascular system. The purpose of this study was to investigate microRNA-320 (miR-320) expression in myocardial ischemia-reperfusion (I/R) injury and the roles of miR-320 in cardiomyocyte apoptosis by targeting AKIP1 (A kinase interacting protein 1). Methods: The level of miR-320 was detected using quantitative real-time polymerase chain reaction (qRT-PCR), and cardiomyocyte apoptosis was detected via terminal dUTP nick end-labeling assay...
2018: Cellular & Molecular Biology Letters
Xinling Zhang, Chunxiang Zhang, Nan Wang, Yan Li, Debing Zhang, Qiang Li
BACKGROUND/AIMS: Acute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODS: H9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC)...
2018: Cellular Physiology and Biochemistry
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