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Rongjie Zhang, Jian Zhou, Jiaxuan Feng, Zhiqing Zhao, Junjun Liu, Zhenjiang Li, Rui Feng, Zaiping Jing
BACKGROUND: How to choose the proximal landing zone in endovascular interventions for a patient with a patent retrograde false lumen in dissection of the ascending aorta and aortic arch remains unclear. This study sought to report the safety and efficiency of inducing thrombosis of the retrograde false lumen to enhance a proximal landing zone to treat retrograde type A aortic dissection. METHODS: This study included 9 patients with retrograde type A aortic dissection treated with a 2-stage operation strategy between January 2015 and January 2016...
September 22, 2016: Journal of Thoracic and Cardiovascular Surgery
Harald Preisner, Eli Levy Karin, Gereon Poschmann, Kai Stühler, Tal Pupko, Sven B Gould
Certain protist lineages bear cytoskeletal structures that are germane to them and define their individual group. Trichomonadida are excavate parasites united by a unique cytoskeletal framework, which includes tubulin-based structures such as the pelta and axostyle, but also other filaments such as the striated costa whose protein composition remains unknown. We determined the proteome of the detergent-resistant cytoskeleton of Tetratrichomonas gallinarum. 203 proteins with homology to Trichomonas vaginalis were identified, which contain significantly more long coiled-coil regions than control protein sets...
September 16, 2016: Protist
Stephanie Preuss, Claudius Kuechle, Stefan Wagenpfeil, Christoph Schmaderer, Lutz Renders, Uwe Heemann, Konrad Stock
The aim of this retrospective single-center study was to examine ultrasound-detected complications after diagnostic parenchymal renal biopsies. After 471 ultrasound-guided kidney biopsies (225 native kidneys and 246 renal allografts), ultrasound revealed hematomas (21.9%), arterio-venous fistulas (8.9%), active bleeding (1.1%) and hematuria (0.4%). Only 0.8% of all patients required invasive intervention such as coiling (n = 3) and surgical procedures (n = 1). Three episodes of bleeding (0.6%) were solved with manual compression...
October 13, 2016: Ultrasound in Medicine & Biology
Chang-Hoon Choi, YongHyun Ha, Pandichelvam Veeraiah, Jörg Felder, Klaus Möllenhoff, N Jon Shah
Non-proton MRI has recently garnered gathering interest with the increased availability of ultra high-field MRI system. Assuming the availability of a broadband RF amplifier, performing multinuclear MR experiments essentially requires additional hardware, such as an RF resonator and a T/R switch for each nucleus. A double- or triple-resonant RF probe is typically constructed using traps or PIN-diode circuits, but this approach degrades the signal-to-noise ratio (SNR) and image quality compared to a single-resonant coil and this is a limiting factor...
October 10, 2016: Journal of Magnetic Resonance
Marios C Yiannakas, Francesco Grussu, Polymnia Louka, Ferran Prados, Rebecca S Samson, Marco Battiston, Daniel R Altmann, Sebastien Ourselin, David H Miller, Claudia A M Gandini Wheeler-Kingshott
Diffusion tensor imaging (DTI) has recently started to be adopted into clinical investigations of spinal cord (SC) diseases. However, DTI applications to the lower SC are limited due to a number of technical challenges, related mainly to the even smaller size of the SC structure at this level, its position relative to the receiver coil elements and the effects of motion during data acquisition. Developing methods to overcome these problems would offer new means to gain further insights into microstructural changes of neurological conditions involving the lower SC, and in turn could help explain symptoms such as bladder and sexual dysfunction...
2016: PloS One
Luca Ferretto, Sandro Irsara
PURPOSE: To describe a sac embolization technique modification for type II endoleak prevention that makes a totally percutaneous approach feasible during endovascular aneurysm repair (EVAR) using the Excluder stent-graft. TECHNIQUE: Percutaneous access of the common femoral arteries is obtained and 2 suture-mediated closure systems are placed on each side. A 16-F or 18-F sheath is placed for delivery of the Excluder stent-graft main body and a 16-F sheath is used on the contralateral side...
October 12, 2016: Journal of Endovascular Therapy
Xiaowei Zhang, Zhifeng Tang, Fuzai Lv, Xiaohong Pan
A wavefront analysis indicates that a flexural wave propagates at a helix angle with respect to the pipe axis. The expression for calculation of the helix angle for each flexural mode is given, and the helix angle dispersion curves for flexural modes are calculated. According to the new understanding of flexural guided waves, a helical comb magnetostrictive patch transducer (HCMPT) is proposed for selectively exciting a single predominant flexural torsional guided wave in a pipe and inspecting spiral welded pipes using flexural waves...
September 29, 2016: Ultrasonics
N De Geeter, P Lioumis, A Laakso, G Crevecoeur, L Dupré
When delivered over a specific cortical site, TMS can temporarily disrupt the ongoing process in that area. This allows mapping of speech-related areas for preoperative evaluation purposes. We numerically explore the observed variability of TMS responses during a speech mapping experiment performed with a neuronavigation system. We selected four cases with very small perturbations in coil position and orientation. In one case (E) a naming error occurred, while in the other cases (NEA, B, C) the subject appointed the images as smoothly as without TMS...
October 7, 2016: Physics in Medicine and Biology
Shiho Sairenji, Shigehisa Akine, Tatsuya Nabeshima
Lanthanide contraction was used for helicity fine-tuning and helix winding control of single-helical tetranuclear complexes LZn3Ln (Ln = La-Lu); heavier lanthanides formed a tighter helix with a higher M/P ratio and gave a more abundant partially-coiled structure, resulting in 14-step structural control of the helical complexes.
September 27, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Patrick Commins, Israel Tilahun Desta, Durga Prasad Karothu, Manas K Panda, Panče Naumov
When exposed to external stimuli such as heat or light, certain single crystals can acquire momentum and undergo motion. On a molecular scale, the motility of such dynamic single crystals is triggered by a phase transition or chemical reaction without gaseous products, and macroscopically manifests as either slow (reversible or irreversible) deformation, or as rapid, almost instantaneous propulsion of the crystals that is oftentimes accompanied by disintegration. While the elastic energy of the slow reconfiguration processes such as bending, twisting and coiling can be utilized for actuation of other objects, the fast disintegrative processes could be exploited to initiate pressure-sensitive applications...
September 29, 2016: Chemical Communications: Chem Comm
Brett Cronin, Robin Robbins, Timothy Maus
OBJECTIVE: To assess the feasibility of pulmonary artery catheter placement using transesophageal echocardiography inclusive of a description of the technique. DESIGN: A prospective, proof-of-concept study. SETTING: Single university hospital. PARTICIPANTS: Twenty patients with chronic thromboembolic pulmonary hypertension scheduled for pulmonary thromboendarterectomy. INTERVENTIONS: Pulmonary artery catheters were placed in 20 patients solely by transesophageal echocardiographic guidance...
July 12, 2016: Journal of Cardiothoracic and Vascular Anesthesia
Eran Leshem, Mahmoud Suleiman, Avishag Laish-Farkash, Yuval Konstantino, Michael Glikson, Alon Barsheshet, Ilan Goldenberg, Yoav Michowitz
AIMS: Dual-coil leads were traditionally considered standard of care due to lower defibrillation thresholds (DFT). Higher complication rates during extraction with parallel progression in implantable cardioverter defibrillator (ICD) technology raised questions on dual coil necessity. Prior substudies found no significant outcome difference between dual and single coils, although using higher rates of DFT testing then currently practiced. We evaluated the temporal trends in implantation rates of single- vs...
October 4, 2016: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
Sylvain Alem, Clint J Perry, Xingfu Zhu, Olli J Loukola, Thomas Ingraham, Eirik Søvik, Lars Chittka
Social insects make elaborate use of simple mechanisms to achieve seemingly complex behavior and may thus provide a unique resource to discover the basic cognitive elements required for culture, i.e., group-specific behaviors that spread from "innovators" to others in the group via social learning. We first explored whether bumblebees can learn a nonnatural object manipulation task by using string pulling to access a reward that was presented out of reach. Only a small minority "innovated" and solved the task spontaneously, but most bees were able to learn to pull a string when trained in a stepwise manner...
October 2016: PLoS Biology
Boris Troyanovsky, Vira Bitko, Viktor Pastukh, Brian Fouty, Victor Solodushko
Minimal piggyBac vectors are a modified single-plasmid version of the classical piggyBac delivery system that can be used for stable transgene integration. These vectors have a truncated terminal domain in the delivery cassette and thus, integrate significantly less flanking transposon DNA into host cell chromatin than classical piggyBac vectors. Herein, we test various characteristics of this modified transposon. The integration efficiency of minimal piggyBac vectors was inversely related to the size of both the transposon and the entire plasmid, but inserts as large as 15 kb were efficiently integrated...
October 4, 2016: Molecular Therapy. Nucleic Acids
Wai Ching Veronica Wong, Aurimas Narkevicius, Wing Ying Chow, David G Reid, Rakesh Rajan, Roger A Brooks, Maggie Green, Melinda J Duer
We have prepared mouse fur extensively (13)C,(15)N-labelled in all amino acid types enabling application of 2D solid state NMR techniques which establish covalent and spatial proximities within, and in favorable cases between, residues. (13)C double quantum-single quantum correlation and proton driven spin diffusion techniques are particularly useful for resolving certain amino acid types. Unlike 1D experiments on isotopically normal material, the 2D methods allow the chemical shifts of entire spin systems of numerous residue types to be determined, particularly those with one or more distinctively shifted atoms such as Gly, Ser, Thr, Tyr, Phe, Val, Leu, Ile and Pro...
October 3, 2016: Journal of Biomolecular NMR
Albert Ho Yuen Chiu, Rajalakshmi Ramesh, Jason Wenderoth, Mark Davies, Andrew Cheung
Subarachnoid hemorrhage secondary to rupture of a circumferential dissecting aneurysm continues to be a treatment dilemma. Vessel sacrifice, when possible, continues to be the safest option but in certain cases this is not possible due to lack of collateral supply. In such cases, coil assisted endovascular flow diversion has become a potential option but the requirement for dual antiplatelet therapy in an unsecured intracranial aneurysm continues to raise concern.We present a 48-year-old man with a World Federation of Neurological Surgeons grade 5 subarachnoid hemorrhage, secondary to a ruptured intradural left vertebral artery dissecting aneurysm, who was treated successfully with a pipeline embolization device with Shield technology using aspirin and a single intravenous loading dose of abciximab...
September 28, 2016: Journal of Neurointerventional Surgery
Youshan Li, Huawei Liu, Rui Zhu, Qingyou Xia, Ping Zhao
Previous studies have indicated that most trypsin inhibitor-like cysteine-rich domain (TIL)-type protease inhibitors, which contain a single TIL domain with ten conserved cysteines, inhibit cathepsin, trypsin, chymotrypsin, or elastase. Our recent findings suggest that Cys(2nd) and Cys(6th) were lost from the TIL domain of the fungal-resistance factors in Bombyx mori, BmSPI38 and BmSPI39, which inhibit microbial proteases and the germination of Beauveria bassiana conidia. To reveal the significance of these two missing cysteines in relation to the structure and function of TIL-type protease inhibitors in B...
September 24, 2016: Peptides
Igor Drobnak, Ajasja Ljubetič, Helena Gradišar, Tomaž Pisanski, Roman Jerala
Proteins are highly perfected natural molecular machines, owing their properties to the complex tertiary structures with precise spatial positioning of different functional groups that have been honed through millennia of evolutionary selection. The prospects of designing new molecular machines and structural scaffolds beyond the limits of natural proteins make design of new protein folds a very attractive prospect. However, de novo design of new protein folds based on optimization of multiple cooperative interactions is very demanding...
2016: Advances in Experimental Medicine and Biology
Manal El-Garhy, Ann Cali, Kareem Morsy, Abdel-Rahman Bashtar, Saleh Al Quraishy
Pleistophora macrozoarcidis a microsporidian parasite infecting the muscle tissue of the ocean pout Macrozoarces americanus collected from the Gulf of Maine of the Atlantic Ocean, MA, USA, was morphologically described on the basis of ultrastructural features. Infection was detected as opaque white or rusty brown lesions scattered throughout the musculature of the fish mainly in the region anterior to anus. Transmission electron microscopy showed that in individual parasitized muscle cells, the infection progresses within parasite formed vesicles which are in direct contact with muscle cell elements...
September 24, 2016: Parasitology Research
Wassim Mansour, Laura Capoccia, Pasqualino Sirignano, Nunzio Montelione, Chiara Pranteda, Martina Formiconi, Enrico Sbarigia, Francesco Speziale
PURPOSE: Hypogastric artery (HA) revascularization during endovascular aneurysm repair (EVAR) is still open to debate. Moreover, exclusion-related complication rates reported in literature are not negligible. The aim of this study is to present and analyze the outcomes in patients undergoing EVAR with exclusion of 1 or both HAs at our academic center. METHODS: We retrospectively reviewed our results in patients submitted to EVAR and needing HA exclusion, in terms of perioperative (30-day) and follow-up rates of intestinal and spinal cord ischemia, buttock claudication, buttock skin necrosis, and sexual dysfunction...
October 2016: Vascular and Endovascular Surgery
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