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https://www.readbyqxmd.com/read/28923469/thermodynamics-and-kinetics-of-single-chain-monellin-folding-with-structural-insights-into-specific-collapse-in-the-denatured-state-ensemble
#1
Hiranmay Maity, Govardhan Reddy
Proteins, which behave as random coils in high denaturant concentrations undergo collapse transition similar to polymers on denaturant dilution. We study collapse in the denatured ensemble of single chain monellin (MNEI) using a coarse-grained protein model and molecular dynamics simulations. The model is validated by quantitatively comparing the computed Guanidinium Chloride (GuHCl) and pH-dependent thermodynamic properties of MNEI folding with the experiments. The computed properties such as the fraction of the protein in the folded state and radius of gyration (Rg) as function of [GuHCl] are in good agreement with the experiments...
September 15, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28922450/structural-insight-into-the-xtacc3-xmap215-interaction-from-cd-and-nmr-studies-on-model-peptides
#2
Angélica Partida-Hanon, Miguel A Treviño, Miguel Mompeán, Ma Ángeles Jiménez, Marta Bruix
TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To characterize this interaction at atomic resolution, we have designed a simplified version of the system based on small peptides. Four different peptides have been studied by circular dichroism and nuclear magnetic resonance both singly and in all possible combinations; namely, five peptide pairs and two trios...
September 18, 2017: Biopolymers
https://www.readbyqxmd.com/read/28921867/polymer-cancerostatics-targeted-with-an-antibody-fragment-bound-via-a-coiled-coil-motif-in-vivo-therapeutic-efficacy-against-murine-bcl1-leukemia
#3
Michal Pechar, Robert Pola, Olga Janoušková, Irena Sieglová, Vlastimil Král, Milan Fábry, Barbora Tomalová, Marek Kovář
A BCL1 leukemia-cell-targeted polymer-drug conjugate with a narrow molecular weight distribution consisting of an N-(2-hydroxypropyl)methacrylamide copolymer carrier and the anticancer drug pirarubicin is prepared by controlled radical copolymerization followed by metal-free click chemistry. A targeting recombinant single chain antibody fragment (scFv) derived from a B1 monoclonal antibody is attached noncovalently to the polymer carrier via a coiled coil interaction between two complementary peptides. Two pairs of coiled coil forming peptides (abbreviated KEK/EKE and KSK/ESE) are used as linkers between the polymer-pirarubicin conjugate and the targeting protein...
September 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28917881/structural-characterization-and-rheological-properties-of-a-galactomannan-from-astragalus-gombo-bunge-seeds-harvested-in-algerian-sahara
#4
T Chouana, G Pierre, C Vial, C Gardarin, A Wadouachi, D Cailleu, D Le Cerf, Z Boual, M D Ould El Hadj, P Michaud, C Delattre
A water soluble polysaccharide (WSP) was extracted and purified from Astragalus gombo seeds (Fabaceae) harvested in Septentrional Sahara (Ouargla, Algeria) with a yield of 6.8% (w/w of the dry seed ground). It was characterized by gas chromatography coupled to the mass spectrometry (GC-MS), size exclusion chromatography with Multi-Angle Light Scattering analysis (SEC-MALLS), high-resolution (1)H and (13)C NMR, and rheological measurements. The structural characterization indicated that this WSP fraction is a galactomannan with a mannose/galactose ratio of 1...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28916562/impact-of-coil-price-knowledge-by-the-operator-on-the-cost-of-aneurysm-coiling-a-single-center-study
#5
Stephanos Finitsis, Robert Fahed, Ian Gaulin, Daniel Roy, Alain Weill
BACKGROUND: Endovascular treatment of aneurysms with coils is among the most frequent treatments in interventional neuroradiology, and represents an important expense. Each manufacturer has created several types of coils, with prices varying among brands and coil types. The objective of this study was to assess the impact of cost awareness of the exact price of each coil by the operating physician on the total cost of aneurysm coiling. MATERIALS AND METHODS: This was a comparative study conducted over 1 year in a single tertiary care center...
September 15, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28916358/the-functional-characterization-and-comparison-of-two-single-crd-containing-c-type-lectins-with-novel-and-typical-key-motifs-from-portunus-trituberculatus
#6
Mengmeng Huang, Changkao Mu, Yuehong Wu, Fei Ye, Dan Wang, Cong Sun, Zhengbing Lv, Bingnan Han, Chunlin Wang, Xue-Wei Xu
C-type lectins are a superfamily of Ca(2+)-dependent carbohydrate-recognition proteins, which play crucial roles in innate immunity including nonself-recognition and pathogen elimination. In the present study, two single-CRD containing C-type lectins were identified from swimming crab Portunus trituberculatus (designated as PtCTL-2 and PtCTL-3). The open reading frame (ORF) of PtCTL-2 encoded polypeptides of 485 amino acids with a signal peptide and a single carbohydrate-recognition domain (CRD), while PtCTL-3's ORF encoded polypeptides of 241 amino acids with a coiled-coil region and a single-CRD...
September 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28913784/managing-wastes-as-green-resources-cigarette-butt-synthesized-pesticides-are-highly-toxic-to-malaria-vectors-with-little-impact-on-predatory-copepods
#7
Kadarkarai Murugan, Udaiyan Suresh, Chellasamy Panneerselvam, Rajapandian Rajaganesh, Mathath Roni, Al Thabiani Aziz, Jiang-Shiou Hwang, Kuppusamy Sathishkumar, Aruliah Rajasekar, Suresh Kumar, Abdullah A Alarfaj, Akon Higuchi, Giovanni Benelli
The development of novel mosquito control tools is a key prerequisite to build effective and reliable Integrated Vector Management strategies. Here, we proposed a novel method using cigarette butts for the synthesis of Ag nanostructures toxic to young instars of the malaria vector Anopheles stephensi, chloroquine (CQ)-resistant malaria parasites Plasmodium falciparum and microbial pathogens. The non-target impact of these nanomaterials in the aquatic environment was evaluated testing them at sub-lethal doses on the predatory copepod Mesocyclops aspericornis...
September 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28913733/a-prospective-feasibility-trial-of-a-novel-intravascular-catheter-system-with-retractable-coiled-tip-guidewire-placed-in-difficult-intravascular-access-diva-patients-in-the-emergency-department
#8
Christopher Raio, Robert Elspermann, Natwalee Kittisarapong, Brendon Stankard, Tanya Bajaj, Veena Modayil, Mathew Nelson, Gerardo Chiricolo, Benjamin Wie, Alexandra Snock, Michael Mackay, Adam Ash
The primary study objective was to evaluate insertion success rates. Secondary objectives included patient satisfaction, procedure time, complication rates, completion of therapy and dwell time of the novel AccuCath(®) 2.25″ Blood Control (BC) Catheter System (FDA approved) placed in difficult-access patients. This was a single-arm feasibility trial evaluating the AccuCath(®) 2.25″ BC Catheter System in a convenience sample of DIVA patients defined as at least two failed initial attempts or a history of difficult access plus the inability to directly visualize or palpate a target vein...
September 14, 2017: Internal and Emergency Medicine
https://www.readbyqxmd.com/read/28906567/self-calibrating-wave-encoded-variable-density-single-shot-fast-spin-echo-imaging
#9
Feiyu Chen, Valentina Taviani, Jonathan I Tamir, Joseph Y Cheng, Tao Zhang, Qiong Song, Brian A Hargreaves, John M Pauly, Shreyas S Vasanawala
BACKGROUND: It is highly desirable in clinical abdominal MR scans to accelerate single-shot fast spin echo (SSFSE) imaging and reduce blurring due to T2 decay and partial-Fourier acquisition. PURPOSE: To develop and investigate the clinical feasibility of wave-encoded variable-density SSFSE imaging for improved image quality and scan time reduction. STUDY TYPE: Prospective controlled clinical trial. SUBJECTS: With Institutional Review Board approval and informed consent, the proposed method was assessed on 20 consecutive adult patients (10 male, 10 female, range, 24-84 years)...
September 14, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28906352/clinical-and-angiographic-outcomes-following-endovascular-treatment-of-very-small-3%C3%A2-mm-or-smaller-intracranial-aneurysm-a-single-center-experience
#10
Yongsheng Liu, Feng Wang, Xiaochen Fu, Yongjian Liu, Guodong Zhang, Ke Xu
Treatments for very small (3 mm or smaller) intracranial aneurysms (VSAs) remain controversial. The aim of this study was to evaluate the efficacy of endovascular treatment for VSAs and to evaluate clinical risk factors associated with complications.This retrospective study enrolled 82 VSA patients who underwent coil embolization in our institution. Angiographic outcomes were assessed according to the Meyers classification. The clinical results were evaluated using the modified Rankin scale (mRS) immediately after coiling, at discharge, and during follow-up...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28898511/mutations-in-the-major-gas-vesicle-protein-gvpa-and-impacts-on-gas-vesicle-formation-in-haloferax-volcanii
#11
Regine Knitsch, Marie Schneefeld, Kerstin Weitzel, Felicitas Pfeifer
Gas vesicles are proteinaceous, gas-filled nanostructures produced by some bacteria and archaea. The hydrophobic major structural protein GvpA forms the ribbed gas vesicle wall. An in-silico 3D-model of GvpA of the predicted coil-α1-β1-β2-α2-coil structure is available and implies that the two β-chains constitute the hydrophobic interior surface of the gas vesicle wall. To test the importance of individual amino acids in GvpA we performed 85 single substitutions and analysed these variants in Haloferax volcanii ΔA+Amut transformants for their ability to form gas vesicles (Vac(+) phenotype)...
September 12, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28893745/a-new-risk-locus-in-chchd5-for-hypertension-and-obesity-in-a-chinese-child-population-a-cohort-study
#12
Lijun Wu, Liwang Gao, Xiaoyuan Zhao, Meixian Zhang, Jianxin Wu, Jie Mi
OBJECTIVE: Coiled-coil-helix-coiled-coil-helix domain containing 5 (CHCHD5), a mitochondrial protein, is involved in the oxidative folding process in the mitochondrial intermembrane space. A previous study identified a hypertension-related single nucleotide polymorphism (SNP), rs3748024, in CHCHD5 in adults, but there are no reports regarding the association between CHCHD5 and obesity, which is a known risk factor for hypertension. The aim of the present study is to investigate the associations of the SNP rs3748024 with hypertension and obesity...
September 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28887383/atspx1-affects-the-atphr1-dna-binding-equilibrium-by-binding-monomeric-atphr1-in-solution
#13
Wanjun Qi, Iain W Manfield, Stephen P Muench, Alison Baker
Phosphorus is an essential macronutrient for plant growth, and is deficient in about 50% of agricultural soils. The transcription factor Phosphate Starvation Response 1 (PHR1) plays a central role in regulating the expression of a subset of Phosphate Starvation Induced (PSI) genes through binding to a cis acting DNA element termed P1BS. In Arabidopsis and rice, activity of AtPHR1/OsPHR2 is regulated in part by their downstream target SPX proteins through protein-protein interaction. Here we provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites...
September 8, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28882993/the-condensin-complex-is-a-mechanochemical-motor-that-translocates-along-dna
#14
Tsuyoshi Terakawa, Shveta Bisht, Jorine M Eeftens, Cees Dekker, Christian H Haering, Eric C Greene
Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. Here, we use single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of ATP hydrolysis-dependent translocation along double-stranded DNA. Condensin's translocation activity is rapid and highly processive, with individual complexes traveling an average distance of ≥10 kilobases at a velocity of ~60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its ~50-nanometer coiled-coil subunits, suggestive of a translocation mechanism that is distinct from any reported DNA motor protein...
September 7, 2017: Science
https://www.readbyqxmd.com/read/28882897/the-tiam1-guanine-nucleotide-exchange-factor-is-auto-inhibited-by-its-pleckstrin-homology-coiled-coil-extension-domain
#15
Zhen Xu, Lokesh Gakhar, Fletcher E Bain, Maria Spies, Ernesto J Fuentes
T-cell lymphoma invasion and metastasis 1 (Tiam1) is a Dbl-family guanine nucleotide exchange factor (GEF) that specifically activates the Rho-family GTPase Rac1 in response to upstream signals, thereby regulating cellular processes including cell adhesion and migration. Tiam1 contains multiple domains, including an N-terminal Pleckstrin homology coiled-coiled extension (PHn-CC-Ex) and catalytic Dbl-homology and C-terminal Pleckstrin homology (DH-PHc) domain. Previous studies indicate that larger fragments of Tiam1, such the region encompassing the N-terminal to C-terminal Pleckstrin homology domains (PHn-PHc) are auto-inhibited...
September 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28882859/flow-diversion-with-low-profile-braided-stents-for-the-treatment-of-very-small-or-uncoilable-intracranial-aneurysms-at-or-distal-to-the-circle-of-willis
#16
K Aydin, M Barburoglu, S Sencer, M Berdikhojayev, B Coskun, S Akpek
BACKGROUND AND PURPOSE: The endovascular treatment of aneurysms located at or distal to the circle of Willis and not amenable to coiling remains a challenge. We report our experience with flow-diversion treatment using low-profile braided stents as a stent monotherapy procedure for treating distally located very small or uncoilable aneurysms. MATERIALS AND METHODS: We retrospectively reviewed our data bases to identify patients with aneurysms located at or distal to the circle of Willis who were treated with stent monotherapy using low-profile braided stents...
September 7, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28882858/web-treatment-of-ruptured-intracranial-aneurysms-a-single-center-cohort-of-100-patients
#17
S B T van Rooij, W J van Rooij, J P Peluso, M Sluzewski, R S Bechan, H G Kortman, G N Beute, B van der Pol, C B Majoie
BACKGROUND AND PURPOSE: The Woven EndoBridge device was recently introduced for the intrasaccular treatment of wide-neck aneurysms without the need for adjunctive devices. We present our results of the primary treatment of ruptured aneurysms with the Woven EndoBridge regardless of location or neck size. MATERIALS AND METHODS: Between February 2015 and April 2017, 100 ruptured aneurysms were selectively treated with the Woven EndoBridge. No supporting stents or balloons were used...
September 7, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28880154/micromagnetic-stimulation-of-the-mouse-auditory-cortex-in-vivo-using-an-implantable-solenoid-system
#18
Shunsuke Minusa, Hisayuki Osanai, Takashi Tateno
OBJECTIVE: Recent studies have reported that micromagnetic stimulation (μMS), which can activate neural tissue and cells via sub-millimeter inductors, may address several limitations of conventional magnetic stimulation methods. Previous studies have examined the effects of μMS on single neurons, yet little is known about how μMS can affect brain tissue including local neural networks. Here, we propose a new, readily available implantable μMS system and computationally and experimentally evaluate its validity...
September 1, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28877692/the-use-of-a-p-falciparum-specific-coiled-coil-domain-to-construct-a-self-assembling-protein-nanoparticle-vaccine-to-prevent-malaria
#19
Christopher P Karch, Tais A P F Doll, Sara M Paulillo, Issa Nebie, David E Lanar, Giampietro Corradin, Peter Burkhard
BACKGROUND: The parasitic disease malaria remains a major global public health concern and no truly effective vaccine exists. One approach to the development of a malaria vaccine is to target the asexual blood stage that results in clinical symptoms. Most attempts have failed. New antigens such as P27A and P27 have emerged as potential new vaccine candidates. Multiple studies have demonstrated that antigens are more immunogenic and are better correlated with protection when presented on particulate delivery systems...
September 6, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28868564/nanoconfinement-greatly-speeds-up-the-nucleation-and-the-annealing-in-single-dna-collapse
#20
Liang Dai, Jeremy J Jones, Alexander R Klotz, Stephen Levy, Patrick S Doyle
Manipulating and measuring single-molecule dynamics and reactions in nanofluidics is a rapidly growing field with broad applications in developing new biotechnologies, understanding nanoconfinement effects in vivo, and exploring new phenomena in confinement. In this work, we investigate the kinetics of DNA collapse in nanoslits using single T4-DNA (165.6 kbp) and λ-DNA (48.5 kbp), with particular focus on the measurement of the nucleation and annealing times. Fixing the ethanol concentration at 35% and varying the slit height from 2000 to 31 nm, the nucleation time dramatically decreases from more than 1 hour to a few minutes or less...
September 4, 2017: Soft Matter
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