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https://www.readbyqxmd.com/read/28444958/role-of-cytochrome-p450-3a4-and-1a2-phenotyping-in-patients-with-advanced-non-small-cell-lung-cancer-nsclc-receiving-erlotinib-treatment
#1
Zinnia P Parra-Guillen, Peter B Berger, Manuel Haschke, Massimiliano Donzelli, Daria Winogradova, Bogumila Pfister, Martin Früh, Charlotte Kloft, Stephan Krähenbühl, Silke Gillessen, Markus Joerger
Erlotinib is metabolized by cytochrome p450 (CYP) 3A and CYP1A. This study assessed CYP3A4 (midazolam) and CYP1A2 (caffeine) phenotyping in plasma and dried blood spots (DBS) for predicting the pharmacokinetics and toxicity of erlotinib in 36 patients with advanced NSCLC. On day 1, erlotinib 150 mg OD. was initiated, and the 2 oral probe drugs midazolam (2mg) and caffeine (100mg) were added on day 1. Plasma and DBS were collected for erlotinib, OSI-420 and probe drugs for up to 6 hr on day 1 and 2-weekly up to week 10...
April 26, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28439866/mapping-protein-protein-interaction-using-high-throughput-yeast-2-hybrid
#2
Jessica Lopez, M Shahid Mukhtar
A tremendous asset to the analysis of protein-protein interactions is the yeast-2-hybrid (Y2H) method. The Y2H assay is a heterologous system that is expanding network biology knowledge via in vivo investigations of binary protein-protein interactions. Traditionally, the Y2H protocol entails the mating or co-transformation of yeast in solid agar media followed by visual analysis for diploid selection. Having played a key role in identifying protein-protein interactions for nearly three decades in a wide range of biological systems, the Y2H system assays the interaction between two proteins of interest which results in a reconstituted and/or activation of transcription factor allowing a reporter gene to be transcribed...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28415902/metabolism-studies-on-hydroxygenkwanin-and-genkwanin-in-human-liver-microsomes-by-uhplc-q-tof-ms
#3
Lin Yuan, Caijuan Liang, Xinpeng Diao, Xiaoye Cheng, Man Liao, Lantong Zhang
Hydroxygenkwanin (HYGN) and genkwanin (GN) are major constituents of Genkwa Flos for the treatment of edema, ascites, cough, asthma and cancer. This is a report about the investigation of the metabolic fate of HYGN and GN in human liver microsomes and the recombinant UDP-glucuronosyltransferase (UGT) enzymes by using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). An on-line data acquisition method multiple mass defect filter (MMDF) combined with dynamic background subtraction (DBS) was developed to trace all probable metabolites...
May 5, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28087071/development-of-delineation-for-the-left-anterior-descending-coronary-artery-region-in-left-breast-cancer-radiotherapy-an-optimized-organ-at-risk
#4
Jie Lee, Kai-Lung Hua, Shih-Ming Hsu, Jhen-Bin Lin, Chou-Hsien Lee, Kuo-Wei Lu, Kun-Yao Dai, Xu-Nian Huang, Jun-Zhao Huang, Meng-Hao Wu, Yu-Jen Chen
BACKGROUND AND PURPOSE: The left anterior descending coronary artery (LAD) and diagonal branches (DBs) are blurred on computed tomography (CT). We aimed to define the LAD region (LADR) with adequate inclusion of the LAD and DBs and contouring consistency. METHODS AND MATERIALS: The LADR was defined using coronary CT angiograms. The inclusion ratio was used to assess the LAD and DBs inclusion by the LADR. Four radiation oncologists delineated the LAD and LADR, using contrast-enhanced CT of 15 patients undergoing left breast radiotherapy...
March 2017: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/28079299/a-platinum-blue-complex-exerts-its-cytotoxic-activity-via-dna-damage-and-induces-apoptosis-in-cancer-cells
#5
Zelal Adiguzel, Seniz Ozalp-Yaman, Gokalp Celik, Safia Salem, Tugba Bagci-Onder, Filiz Senbabaoglu, Yüksel Cetin, Ceyda Acilan
Here, we describe the characteristics of a Pt-blue complex [Pt4 (2-atp)8 (H2 O)(OH)] (2-atp: 2-aminothiophenol) as a prodrug for its DNA-binding properties and its use in cancer therapy. The nature of the interaction between the Pt-blue complex and DNA was evaluated based on spectroscopic measurements, the electronic absorption spectra, thermal behavior, viscosity, fluorometric titration and agarose gel electrophoresis. Our results suggested that the compound was able to partially intercalate DNA and appeared to induce both single and double stranded breaks (DBS) on DNA in vitro, but no DSBs in cells...
January 12, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28044937/cytokine-status-of-serum-in-ovarian-cancer-patients-with-different-tumor-neoadjuvant-chemotherapy-response
#6
Inna I Antoneeva, Tatyana V Abakumova, Dinara R Dolgova, Tatyana P Gening, Sabina S Pirmamedova, Dinara F Myasnikova, Snezhanna O Gening
The purpose of the study was to evaluate the cytokine status in the blood serum of ovarian cancer (OC) patients with different tumor neoadjuvant chemotherapy (NACT) response. We determined the levels of IL-1β, -1Ra, -10, TNF-α by enzyme-linked immunosorbent assay in the blood serum of 97 patients with primary ovarian cancer and 64 healthy women. Analysis of polymorphic variants of specific sections of cytokine genes was performed using allele- specific PCR. Polymorphic variants were selected within the promoters of IL-1β T- 31C gene (rs 1143627 ), TNF-α G-308A (rs 1800629) and IL-10 G-1082A (rs 1800896), presented in dbS`NP NCBI and SNP500 Cancer databases...
January 2, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/27694690/suppression-of-pc-1-prlz-sensitizes-prostate-cancer-cells-to-ionizing-radiation-by-attenuating-dna-damage-repair-and-inducing-autophagic-cell-death
#7
Zeng-Fu Shang, Qiang Wei, Lan Yu, Fang Huang, Bei-Bei Xiao, Hongtao Wang, Man Song, Li Wang, Jianguang Zhou, Jian Wang, Shanhu Li
Radiotherapy is promising and effective for treating prostate cancer but the addition of a tumor cell radiosensitizer would improve therapeutic outcomes. PC-1/PrLZ, a TPD52 protein family member is frequently upregulated in advanced prostate cancer cells and may be a biomarker of aggressive prostate cancer. Therefore, we investigated the potential role of PC-1/PrLZ for increasing radioresistance in human prostate cancer cell lines. Growth curves and survival assays after g-ray irradiation confirmed that depletion of endogenous PC-1/PrLZ significantly increased prostate cancer cell radiosensitivity...
September 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27317883/reactivation-of-mutant-p53-constraints-on-mechanism-highlighted-by-principal-component-analysis-of-the-dna-binding-domain
#8
Zahra Ouaray, Karim M ElSawy, David P Lane, Jonathan W Essex, Chandra Verma
Most p53 mutations associated with cancer are located in its DNA binding domain (DBD). Many structures (X-ray and NMR) of this domain are available in the protein data bank (PDB) and a vast conformational heterogeneity characterizes the various free and complexed states. The major difference between the apo and the holo-complexed states appears to lie in the L1 loop. In particular, the conformations of this loop appear to depend intimately on the sequence of DNA to which it binds. This conclusion builds upon recent observations that implicate the tetramerization and the C-terminal domains (respectively TD and Cter) in DNA binding specificity...
October 2016: Proteins
https://www.readbyqxmd.com/read/27217771/tandem-mass-spectrometry-based-newborn-screening-strategy-could-be-used-to-facilitate-rapid-and-sensitive-lung-cancer-diagnosis
#9
Ting Huang, Yunfeng Cao, Jia Zeng, Jun Dong, Xiaoyu Sun, Jianxing Chen, Peng Gao
OBJECTIVE: Newborn screening (NBS) helps in the early detection of inborn errors of metabolism (IEM). The most effective NBS strategy prevailing in clinics is tandem mass spectrometry (MS/MS) analysis using dried blood spot (DBS) samples. Taking lung cancer (LC) as an example, this study tried to explore if this technique could be of any assistance for the discovery of tumor metabolite markers. MATERIALS AND METHODS: Twenty-six acylcarnitines and 23 amino acids, which are commonly used in IEM screening, were quantified using DBS samples from 222 LC patients, 118 benign lung disease (LD) patients, and 96 healthy volunteers (CONT)...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27210055/conductive-paper-with-antibody-like-film-for-electrical-readings-of-biomolecules
#10
Ana P M Tavares, Nádia S Ferreira, Liliana A A N A Truta, M Goreti F Sales
This work reports a novel way of producing an inexpensive substrate support to assemble a sensing film, designed for the electrical transduction of an intended biomolecule. The support uses cellulose paper as substrate, made hydrophobic with solid wax and covered by a home-made conductive ink having graphite as core material. The hydrophobicity of the paper was confirmed by contact angle measurements and the conductive ink composition was optimized with regard to its adhesion, conductivity, and thermal stability...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27170433/self-collected-dried-blood-spots-as-a-tool-for-measuring-ovarian-reserve-in-young-female-cancer-survivors
#11
S C Roberts, S M Seav, T W McDade, S A Dominick, J R Gorman, B W Whitcomb, H I Su
STUDY QUESTION: Are female young cancer survivors (YCS) able to self-collect high-quality dried blood spots (DBSs) at home to provide biospecimens for studying ovarian reserve? SUMMARY ANSWER: YCS can self-collect high-quality DBS specimens in non-clinical settings, and anti-Mullerian hormone (AMH) levels can be assayed in such specimens. WHAT IS KNOWN ALREADY: Large-scale biosample collection is a barrier to studying ovarian reserve in YCS...
July 2016: Human Reproduction
https://www.readbyqxmd.com/read/27029223/gamma-knife-radiosurgery-in-movement-disorders-indications-and-limitations
#12
REVIEW
Yoshinori Higuchi, Shinji Matsuda, Toru Serizawa
Functional radiosurgery has advanced steadily during the past half century since the development of the gamma knife technique for treating intractable cancer pain. Applications of radiosurgery for intracranial diseases have increased with a focus on understanding radiobiology. Currently, the use of gamma knife radiosurgery to ablate deep brain structures is not widespread because visualization of the functional targets remains difficult despite the increased availability of advanced neuroimaging technology...
January 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/26884234/prognostic-value-of-the-distance-between-the-primary-tumor-and-brainstem-in-the-patients-with-locally-advanced-nasopharyngeal-carcinoma
#13
Yuxiang He, Ying Wang, Lin Shen, Yajie Zhao, Pengfei Cao, Mingjun Lei, Dengming Chen, Tubao Yang, Liangfang Shen, Shousong Cao
BACKGROUND: Brainstem dose limitations influence radiation dose reaching to tumor in the patients with locally-advanced nasopharyngeal cancer (NPC). METHODS: A retrospective analysis of the prognostic value of the distance between the primary tumor and brainstem (Dbs) in 358 patients with locally-advanced NPC after intensity-modulated radiation therapy (IMRT). Receiver operating characteristic (ROC) curves were used to identify the cut-off value to analyze the impact of Dbs on tumor dose coverage and prognosis...
February 17, 2016: BMC Cancer
https://www.readbyqxmd.com/read/26514985/a-simple-dried-blood-spot-method-for-clinical-pharmacological-analyses-of-etoposide-in-cancer-patients-using-liquid-chromatography-and-fluorescence-detection
#14
Renata Režonja Kukec, Iztok Grabnar, Aleš Mrhar, Nanča Čebron Lipovec, Tanja Čufer, Tomaž Vovk
BACKGROUND: Therapeutic drug monitoring of etoposide is not part of the routine clinical practice, however, measuring etoposide plasma concentration may be useful to prevent chemotherapy related adverse drug reactions. This paper describes the development and validation of a dried blood spot (DBS) assay for the determination of etoposide in blood samples of lung cancer patients. METHODS: The whole blood spot was cut out of the DBS card followed by sonication assisted liquid drug extraction...
January 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/26315009/report-of-whole-brain-radiation-therapy-in-a-patient-with-an-implanted-deep-brain-stimulator-important-neurosurgical-considerations-and-radiotherapy-practice-principles
#15
Rupesh Kotecha, Camille A Berriochoa, Erin S Murphy, Andre G Machado, Samuel T Chao, John H Suh, Kevin L Stephans
Patients with implanted neuromodulation devices present potential challenges for radiation therapy treatment planning and delivery. Although guidelines exist regarding the irradiation of cardiac pacemakers and defibrillators, fewer data and less clinical experience exist regarding the effects of radiation therapy on less frequently used devices, such as deep brain stimulators. A 79-year-old woman with a history of coarse tremors effectively managed with deep brain stimulation presented with multiple intracranial metastases from a newly diagnosed lung cancer and was referred for whole-brain radiation therapy...
April 2016: Journal of Neurosurgery
https://www.readbyqxmd.com/read/26195453/a-high-throughput-assay-for-rho-guanine-nucleotide-exchange-factors-based-on-the-transcreener-gdp-assay
#16
Melvin Reichman, Amanda Schabdach, Meera Kumar, Tom Zielinski, Preston S Donover, Lisa D Laury-Kleintop, Robert G Lowery
Ras homologous (Rho) family GTPases act as molecular switches controlling cell growth, movement, and gene expression by cycling between inactive guanosine diphosphate (GDP)- and active guanosine triphosphate (GTP)-bound conformations. Guanine nucleotide exchange factors (GEFs) positively regulate Rho GTPases by accelerating GDP dissociation to allow formation of the active, GTP-bound complex. Rho proteins are directly involved in cancer pathways, especially cell migration and invasion, and inhibiting GEFs holds potential as a therapeutic strategy to diminish Rho-dependent oncogenesis...
December 2015: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/25886002/feasibility-of-self-sampled-dried-blood-spot-and-saliva-samples-sent-by-mail-in-a-population-based-study
#17
Amrit Kaur Sakhi, Nasser Ezzatkhah Bastani, Merete Ellingjord-Dale, Thomas Erik Gundersen, Rune Blomhoff, Giske Ursin
BACKGROUND: In large epidemiological studies it is often challenging to obtain biological samples. Self-sampling by study participants using dried blood spots (DBS) technique has been suggested to overcome this challenge. DBS is a type of biosampling where blood samples are obtained by a finger-prick lancet, blotted and dried on filter paper. However, the feasibility and efficacy of collecting DBS samples from study participants in large-scale epidemiological studies is not known. The aim of the present study was to test the feasibility and response rate of collecting self-sampled DBS and saliva samples in a population-based study of women above 50 years of age...
2015: BMC Cancer
https://www.readbyqxmd.com/read/25709194/long-term-high-dose-oral-morphine-in-phantom-limb-pain-with-no-addiction-risk
#18
Vinod Kumar, Rakesh Garg, Sachidanand Jee Bharati, Nishkarsh Gupta, Sushma Bhatanagar, Seema Mishra, Yatan Pal Singh Balhara
Chronic phantom limb pain (PLP) is a type of neuropathic pain, which is located in the missing/amputated limb. Phantom pain is difficult to treat as the exact basis of pain mechanism is still unknown. Various methods of treatment for PLP have been described, including pharmacological (NSAIDs, opioids, antiepileptic, antidepressants) and non-pharmacological (TENS, sympathectomy, deep brain stimulation and motor cortex stimulation). Opioids are used for the treatment of neuropathic pain and dose of opioid is determined based on its effect and thus there is no defined ceiling dose for opioids...
January 2015: Indian Journal of Palliative Care
https://www.readbyqxmd.com/read/25476377/ultra-high-performance-liquid-chromatography-tandem-mass-spectrometric-method-for-the-determination-of-tamoxifen-n-desmethyltamoxifen-4-hydroxytamoxifen-and-endoxifen-in-dried-blood-spots-development-validation-and-clinical-application-during-breast-cancer
#19
Marina Venzon Antunes, Suziane Raymundo, Vanessa de Oliveira, Dilana Elisabeth Staudt, Gustavo Gössling, Giovana Piva Peteffi, Jorge Villanova Biazús, José Antônio Cavalheiro, Marie Tre-Hardy, Arnaud Capron, Vincent Haufroid, Pierre Wallemacq, Gilberto Schwartsmann, Rafael Linden
A LC-MSMS method for the simultaneous determination of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in dried blood spots samples was developed and validated. The method employs an ultrasound-assisted liquid extraction and a reversed phase separation in an Acquity(®) C18 column (150×2.1 mm, 1.7 µm). Mobile phase was a mixture of formic acid 0.1% (v/v) pH 2.7 and acetonitrile (gradient from 60:40 to 50:50, v/v). Total analytical run time was 8 min. Precision assays showed CV % lower than 10...
January 2015: Talanta
https://www.readbyqxmd.com/read/25409685/a-dna-repair-variant-in-polq-c-1060a-g-is-associated-to-hereditary-breast-cancer-patients-a-case-control-study
#20
Ana Paula Carneiro Brandalize, Lavínia Schüler-Faccini, Jean-Sébastien Hoffmann, Maira Caleffi, Christophe Cazaux, Patricia Ashton-Prolla
BACKGROUND: One of the hallmarks of cancer is the occurrence of high levels of chromosomal rearrangements as a result of inaccurate repair of double-strand breaks (DSB). Germline mutations in BRCA and RAD51 genes, involved in DSB repair, are strongly associated with hereditary breast cancer. Pol θ, a translesional DNA polymerase specialized in the replication of damaged DNA, has been also shown to contribute to DNA synthesis associated to DSB repair. It is noteworthy that POLQ is highly expressed in breast tumors and this expression is able to predict patient outcome...
2014: BMC Cancer
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