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https://www.readbyqxmd.com/read/29222715/clinical-validation-study-of-dried-blood-spot-for-determining-everolimus-concentration-in-patients-with-cancer
#1
A E C A B Willemsen, L M Knapen, Y M de Beer, R J M Brüggemann, S Croes, C M L van Herpen, N P van Erp
PURPOSE: Everolimus treatment is seriously hampered by its toxicity profile. As a relationship between everolimus exposure and effectiveness and toxicity has been established, early and ongoing concentration measurement can be key to individualize the dose and optimize treatment outcomes. Dried blood spot (DBS) facilitates sampling at a patients' home and thereby eases dose individualization. The aim of this study is to determine the agreement and predictive performance of DBS compared to whole blood (WB) to measure everolimus concentrations in cancer patients...
December 8, 2017: European Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29216585/determination-of-irinotecan-and-its-metabolite-sn-38-in-dried-blood-spots-using-high-performance-liquid-chromatography-with-fluorescence-detection
#2
Roberta Z Hahn, Priscila C Arnhold, Natália B Andriguetti, Anelise Schneider, Helena M Klück, Simone L Dos Reis, Marcos Frank Bastiani, Igor Kael, Anne Caroline Cezimbra da Silva, Gilberto Schwartsmann, Marina V Antunes, Rafael Linden
Irinotecan (IRI) is an antineoplastic drug widely used for the treatment of colorectal and advanced pancreatic cancer. Despite its clinical utility, the clinical use of IRI is associated with potentially severe hematopoietic and gastrointestinal toxicities. The quantification of IRI and its active metabolite SN-38 in dried blood spots (DBS) may be an alternative to individualize the drug dose through a minimally invasive and easy collection method. The aim of this study was to develop and validate a simple and fast HPLC-FL assay for simultaneous IRI and SN-38 measurement in DBS, with adequate analytical performance for clinical use...
December 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29112898/development-and-validation-of-an-analytical-method-using-uplc-ms-ms-to-quantify-everolimus-in-dried-blood-spots-in-the-oncology-setting
#3
Lotte M Knapen, Yvo de Beer, Roger J M Brüggemann, Leo M Stolk, Frank de Vries, Vivianne C G Tjan-Heijnen, Nielka P van Erp, Sander Croes
While the therapeutic drug monitoring (TDM) of everolimus has been routinely performed for over 10 years in solid organ transplantation medicine, in order to optimize the balance between effectiveness and toxicity, it is yet uncommon in the treatment of malignancies. The aim of this study was to develop and validate a bioanalytical method to quantify everolimus in dried blood spots (DBS) to facilitate TDM for the oncology outpatient setting. The hematocrit effect of everolimus was investigated. An 7.5mm disk from the central part of the DBS was punched, followed by the extraction of everolimus from the DBS by methanol/acetonitrile (80/20%) spiked with deuterium-labelled everolimus as internal standard...
October 29, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28733137/can-metabolic-tumour-parameters-on-primary-staging-18-f-fdg-pet-ct-contribute-to-risk-stratification-of-primary-central-nervous-system-lymphomas-for-patient-management-as-a-prognostic-model
#4
K Okuyucu, E Alagoz, S Ince, S Ozaydin, N Arslan
OBJECTIVE: Primary central nervous system (CNS) lymphoma is an aggressive and fatal extranodal non-Hodgkin lymphoma (NHL) jailed in CNS at initial diagnosis. Its prognosis is poor and the disease has a fatal outcome when compared with systemic NHL. A few baseline risk stratification scoring systems have been suggested to estimate the prognosis mainly based on serum lactate dehydrogenase (LDH) level, age, Karnofsky performance score (KPS), involvement of deep brain structures (DBS) and cerebrospinal fluid protein concentration...
July 18, 2017: Revista Española de Medicina Nuclear e Imagen Molecular
https://www.readbyqxmd.com/read/28679647/technical-stability-and-biological-variability-in-micrornas-from-dried-blood-spots-a-lung-cancer-therapy-monitoring-showcase
#5
Mustafa Kahraman, Thomas Laufer, Christina Backes, Hannah Schrörs, Tobias Fehlmann, Nicole Ludwig, Jochen Kohlhaas, Eckart Meese, Thomas Wehler, Robert Bals, Andreas Keller
BACKGROUND: Different work flows have been proposed to use miRNAs as blood-borne biomarkers. In particular, the method used for collecting blood from patients can considerably influence the diagnostic results. METHODS: We explored whether dried blood spots (DBSs) facilitate stable miRNA measurements and compared its technical stability with biological variability. First, we tested the stability of DBS samples by generating from 1 person 18 whole-genome-wide miRNA profiles of DBS samples that were exposed to different temperature and humidity conditions...
September 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28444958/role-of-cytochrome-p450-3a4-and-1a2-phenotyping-in-patients-with-advanced-non-small-cell-lung-cancer-receiving-erlotinib-treatment
#6
Zinnia P Parra-Guillen, Peter B Berger, Manuel Haschke, Massimiliano Donzelli, Daria Winogradova, Bogumila Pfister, Martin Früh, Silke Gillessen, Stephan Krähenbühl, Charlotte Kloft, Markus Joerger
Erlotinib is metabolized by cytochrome p450 (CYP) 3A and CYP1A. This study assessed CYP3A4 (midazolam) and CYP1A2 (caffeine) phenotyping in plasma and dried blood spots (DBS) for predicting the pharmacokinetics and toxicity of erlotinib in 36 patients with advanced NSCLC. On day 1, erlotinib 150 mg OD was initiated, and the two oral probe drugs midazolam (2 mg) and caffeine (100 mg) were added on day 1. Plasma and DBS were collected for erlotinib, OSI-420 and probe drugs for up to 6 hr on day 1 and 2-weekly up to week 10...
April 26, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28439866/mapping-protein-protein-interaction-using-high-throughput-yeast-2-hybrid
#7
Jessica Lopez, M Shahid Mukhtar
A tremendous asset to the analysis of protein-protein interactions is the yeast-2-hybrid (Y2H) method. The Y2H assay is a heterologous system that is expanding network biology knowledge via in vivo investigations of binary protein-protein interactions. Traditionally, the Y2H protocol entails the mating or co-transformation of yeast in solid agar media followed by visual analysis for diploid selection. Having played a key role in identifying protein-protein interactions for nearly three decades in a wide range of biological systems, the Y2H system assays the interaction between two proteins of interest which results in a reconstituted and/or activation of transcription factor allowing a reporter gene to be transcribed...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28415902/metabolism-studies-on-hydroxygenkwanin-and-genkwanin-in-human-liver-microsomes-by-uhplc-q-tof-ms
#8
Lin Yuan, Caijuan Liang, Xinpeng Diao, Xiaoye Cheng, Man Liao, Lantong Zhang
Hydroxygenkwanin (HYGN) and genkwanin (GN) are major constituents of Genkwa Flos for the treatment of edema, ascites, cough, asthma and cancer. This is a report about the investigation of the metabolic fate of HYGN and GN in human liver microsomes and the recombinant UDP-glucuronosyltransferase (UGT) enzymes by using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). An on-line data acquisition method multiple mass defect filter (MMDF) combined with dynamic background subtraction (DBS) was developed to trace all probable metabolites...
May 5, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28087071/development-of-delineation-for-the-left-anterior-descending-coronary-artery-region-in-left-breast-cancer-radiotherapy-an-optimized-organ-at-risk
#9
Jie Lee, Kai-Lung Hua, Shih-Ming Hsu, Jhen-Bin Lin, Chou-Hsien Lee, Kuo-Wei Lu, Kun-Yao Dai, Xu-Nian Huang, Jun-Zhao Huang, Meng-Hao Wu, Yu-Jen Chen
BACKGROUND AND PURPOSE: The left anterior descending coronary artery (LAD) and diagonal branches (DBs) are blurred on computed tomography (CT). We aimed to define the LAD region (LADR) with adequate inclusion of the LAD and DBs and contouring consistency. METHODS AND MATERIALS: The LADR was defined using coronary CT angiograms. The inclusion ratio was used to assess the LAD and DBs inclusion by the LADR. Four radiation oncologists delineated the LAD and LADR, using contrast-enhanced CT of 15 patients undergoing left breast radiotherapy...
March 2017: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/28079299/a-platinum-blue-complex-exerts-its-cytotoxic-activity-via-dna-damage-and-induces-apoptosis-in-cancer-cells
#10
Zelal Adiguzel, Seniz Ozalp-Yaman, Gokalp Celik, Safia Salem, Tugba Bagci-Onder, Filiz Senbabaoglu, Yüksel Cetin, Ceyda Acilan
Here, we describe the characteristics of a Pt-blue complex [Pt4 (2-atp)8 (H2 O)(OH)] (2-atp: 2-aminothiophenol) as a prodrug for its DNA-binding properties and its use in cancer therapy. The nature of the interaction between the Pt-blue complex and DNA was evaluated based on spectroscopic measurements, the electronic absorption spectra, thermal behavior, viscosity, fluorometric titration, and agarose gel electrophoresis. Our results suggested that the compound was able to partially intercalate DNA and appeared to induce both single- and double-stranded breaks (DBS) on DNA in vitro, but no DSBs in cells...
August 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28044937/cytokine-status-of-serum-in-ovarian-cancer-patients-with-different-tumor-neoadjuvant-chemotherapy-response
#11
Inna I Antoneeva, Tatyana V Abakumova, Dinara R Dolgova, Tatyana P Gening, Sabina S Pirmamedova, Dinara F Myasnikova, Snezhanna O Gening
BACKGROUND: Polychemotherapy in ovarian cancer (OC) is the second major component of treatment. However, treatment with cytostatics is stopped in 25% of cases because of significant side effects. It is shown that concentration of certain cytokines and their balance is largely formed in accordance with a genetic polymorphism. OBJECTIVE: The objective of the study was to evaluate the cytokine status of blood serum of patients with ovarian cancer with different tumor response to neoadjuvant chemotherapy (NACT)...
2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/27694690/suppression-of-pc-1-prlz-sensitizes-prostate-cancer-cells-to-ionizing-radiation-by-attenuating-dna-damage-repair-and-inducing-autophagic-cell-death
#12
Zeng-Fu Shang, Qiang Wei, Lan Yu, Fang Huang, Bei-Bei Xiao, Hongtao Wang, Man Song, Li Wang, Jianguang Zhou, Jian Wang, Shanhu Li
Radiotherapy is promising and effective for treating prostate cancer but the addition of a tumor cell radiosensitizer would improve therapeutic outcomes. PC-1/PrLZ, a TPD52 protein family member is frequently upregulated in advanced prostate cancer cells and may be a biomarker of aggressive prostate cancer. Therefore, we investigated the potential role of PC-1/PrLZ for increasing radioresistance in human prostate cancer cell lines. Growth curves and survival assays after g-ray irradiation confirmed that depletion of endogenous PC-1/PrLZ significantly increased prostate cancer cell radiosensitivity...
September 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27317883/reactivation-of-mutant-p53-constraints-on-mechanism-highlighted-by-principal-component-analysis-of-the-dna-binding-domain
#13
Zahra Ouaray, Karim M ElSawy, David P Lane, Jonathan W Essex, Chandra Verma
Most p53 mutations associated with cancer are located in its DNA binding domain (DBD). Many structures (X-ray and NMR) of this domain are available in the protein data bank (PDB) and a vast conformational heterogeneity characterizes the various free and complexed states. The major difference between the apo and the holo-complexed states appears to lie in the L1 loop. In particular, the conformations of this loop appear to depend intimately on the sequence of DNA to which it binds. This conclusion builds upon recent observations that implicate the tetramerization and the C-terminal domains (respectively TD and Cter) in DNA binding specificity...
October 2016: Proteins
https://www.readbyqxmd.com/read/27217771/tandem-mass-spectrometry-based-newborn-screening-strategy-could-be-used-to-facilitate-rapid-and-sensitive-lung-cancer-diagnosis
#14
Ting Huang, Yunfeng Cao, Jia Zeng, Jun Dong, Xiaoyu Sun, Jianxing Chen, Peng Gao
OBJECTIVE: Newborn screening (NBS) helps in the early detection of inborn errors of metabolism (IEM). The most effective NBS strategy prevailing in clinics is tandem mass spectrometry (MS/MS) analysis using dried blood spot (DBS) samples. Taking lung cancer (LC) as an example, this study tried to explore if this technique could be of any assistance for the discovery of tumor metabolite markers. MATERIALS AND METHODS: Twenty-six acylcarnitines and 23 amino acids, which are commonly used in IEM screening, were quantified using DBS samples from 222 LC patients, 118 benign lung disease (LD) patients, and 96 healthy volunteers (CONT)...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27210055/conductive-paper-with-antibody-like-film-for-electrical-readings-of-biomolecules
#15
Ana P M Tavares, Nádia S Ferreira, Liliana A A N A Truta, M Goreti F Sales
This work reports a novel way of producing an inexpensive substrate support to assemble a sensing film, designed for the electrical transduction of an intended biomolecule. The support uses cellulose paper as substrate, made hydrophobic with solid wax and covered by a home-made conductive ink having graphite as core material. The hydrophobicity of the paper was confirmed by contact angle measurements and the conductive ink composition was optimized with regard to its adhesion, conductivity, and thermal stability...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27170433/self-collected-dried-blood-spots-as-a-tool-for-measuring-ovarian-reserve-in-young-female-cancer-survivors
#16
S C Roberts, S M Seav, T W McDade, S A Dominick, J R Gorman, B W Whitcomb, H I Su
STUDY QUESTION: Are female young cancer survivors (YCS) able to self-collect high-quality dried blood spots (DBSs) at home to provide biospecimens for studying ovarian reserve? SUMMARY ANSWER: YCS can self-collect high-quality DBS specimens in non-clinical settings, and anti-Mullerian hormone (AMH) levels can be assayed in such specimens. WHAT IS KNOWN ALREADY: Large-scale biosample collection is a barrier to studying ovarian reserve in YCS...
July 2016: Human Reproduction
https://www.readbyqxmd.com/read/27029223/gamma-knife-radiosurgery-in-movement-disorders-indications-and-limitations
#17
REVIEW
Yoshinori Higuchi, Shinji Matsuda, Toru Serizawa
Functional radiosurgery has advanced steadily during the past half century since the development of the gamma knife technique for treating intractable cancer pain. Applications of radiosurgery for intracranial diseases have increased with a focus on understanding radiobiology. Currently, the use of gamma knife radiosurgery to ablate deep brain structures is not widespread because visualization of the functional targets remains difficult despite the increased availability of advanced neuroimaging technology...
January 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/26884234/prognostic-value-of-the-distance-between-the-primary-tumor-and-brainstem-in-the-patients-with-locally-advanced-nasopharyngeal-carcinoma
#18
Yuxiang He, Ying Wang, Lin Shen, Yajie Zhao, Pengfei Cao, Mingjun Lei, Dengming Chen, Tubao Yang, Liangfang Shen, Shousong Cao
BACKGROUND: Brainstem dose limitations influence radiation dose reaching to tumor in the patients with locally-advanced nasopharyngeal cancer (NPC). METHODS: A retrospective analysis of the prognostic value of the distance between the primary tumor and brainstem (Dbs) in 358 patients with locally-advanced NPC after intensity-modulated radiation therapy (IMRT). Receiver operating characteristic (ROC) curves were used to identify the cut-off value to analyze the impact of Dbs on tumor dose coverage and prognosis...
February 17, 2016: BMC Cancer
https://www.readbyqxmd.com/read/26514985/a-simple-dried-blood-spot-method-for-clinical-pharmacological-analyses-of-etoposide-in-cancer-patients-using-liquid-chromatography-and-fluorescence-detection
#19
Renata Režonja Kukec, Iztok Grabnar, Aleš Mrhar, Nanča Čebron Lipovec, Tanja Čufer, Tomaž Vovk
BACKGROUND: Therapeutic drug monitoring of etoposide is not part of the routine clinical practice, however, measuring etoposide plasma concentration may be useful to prevent chemotherapy related adverse drug reactions. This paper describes the development and validation of a dried blood spot (DBS) assay for the determination of etoposide in blood samples of lung cancer patients. METHODS: The whole blood spot was cut out of the DBS card followed by sonication assisted liquid drug extraction...
January 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/26315009/report-of-whole-brain-radiation-therapy-in-a-patient-with-an-implanted-deep-brain-stimulator-important-neurosurgical-considerations-and-radiotherapy-practice-principles
#20
Rupesh Kotecha, Camille A Berriochoa, Erin S Murphy, Andre G Machado, Samuel T Chao, John H Suh, Kevin L Stephans
Patients with implanted neuromodulation devices present potential challenges for radiation therapy treatment planning and delivery. Although guidelines exist regarding the irradiation of cardiac pacemakers and defibrillators, fewer data and less clinical experience exist regarding the effects of radiation therapy on less frequently used devices, such as deep brain stimulators. A 79-year-old woman with a history of coarse tremors effectively managed with deep brain stimulation presented with multiple intracranial metastases from a newly diagnosed lung cancer and was referred for whole-brain radiation therapy...
April 2016: Journal of Neurosurgery
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