Juliana A Camargo, Nayara I Viana, Ruan Pimenta, Vanessa R Guimarães, Gabriel A Dos Santos, Patrícia Candido, Vitória Ghazarian, Poliana Romão, Iran A Silva, Alexander Birbrair, Miguel Srougi, William C Nahas, Kátia R Leite, Ericka B Trarbach, Sabrina T Reis
Prostate cancer (PCa) has a high prevalence and represents an important health problem, with an increased risk of metastasis. With the advance of CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 and miR-21 target genes are associated with PCa progression; therefore, we evaluated the MMP-9 and miR-21 targets in PCa using the CRISPR-Cas9 system. Single guide RNAs (sgRNAs) of MMP9 and miR-21 sequences were inserted into a PX-330 plasmid, and transfected in DU145 and PC-3 PCa cell lines...
October 3, 2023: International Journal of Molecular Sciences