keyword
MENU ▼
Read by QxMD icon Read
search

Mcr 1

keyword
https://www.readbyqxmd.com/read/29778043/investigation-of-interactions-of-comtan-with-human-serum-albumin-by-mathematically-modeled-voltammetric-data-a-study-from-bio-interaction-to-biosensing
#1
Ali R Jalalvand, Sirous Ghobadi, Hector C Goicoechea, Hui-Wen Gu, Esmael Sanchooli
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable...
May 16, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29774212/alkaline-peptone-water-based-enrichment-method-for-mcr-3-from-acute-diarrheic-outpatient-gut-samples
#2
Qiaoling Sun, Yanyan Hu, Hongwei Zhou, Lingbin Shu, Hanyu Wang, Zixian Huang, Rong Zhang
A third plasmid-mediated colistin resistance gene, mcr-3 , is increasingly being reported in Enterobacteriaceae and Aeromonas spp. from animals and humans. To investigate the molecular epidemiology of mcr in the gut flora of Chinese outpatients, 152 stool specimens were randomly collected from outpatients in our hospital from May to June, 2017. Stool specimens enriched in alkaline peptone water or Luria-Bertani (LB) broth were screened for mcr-1, mcr-2 , and mcr-3 using polymerase chain reaction (PCR)-based assays...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29768099/the-mcr-1-gene-has-no-effect-on-colistin-resistance-when-it-coexists-with-inactivated-mgrb-gene-in-klebsiella-pneumoniae
#3
Huichuan Zhang, Dongdong Zhao, Qiucheng Shi, Jingjing Quan, Xi Li, Yunsong Yu
The inactivated mgrB gene and the mcr-1 gene are important mechanisms of colistin resistance in Klebsiella pneumoniae and they are threats to the clinical use of colistin. In this study, mcr-1 gene was cloned into K. pneumoniae strains (XH209 and KP10) and their derived strains (XH209 M and KP10 M), which showed high-level resistance to colistin. The acquisition of the mcr-1 gene led to colistin resistance in XH209 and KP10, but the addition of mcr-1 gene did not cause change of colistin minimum inhibitory concentrations in the XH209 M and KP10 M...
May 16, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29760138/co-location-of-the-polymyxin-resistance-gene-mcr-1-and-variant-of-mcr-3-on-a-plasmid-in-escherichia-coli-from-chicken-farm
#4
Rong Xiang, Bi-Hui Liu, An-Yun Zhang, Chang-Wei Lei, Xiao-Lan Ye, Yan-Xian Yang, Yan-Peng Chen, Hong-Ning Wang
A colistin-resistant Escherichia coli from commercial poultry farm in China carried two colistin-resistance genes mcr-1 and variant of mcr-3 in an IncP plasmid. The variant of mcr-3 gene, named mcr-3.11 , encoded two amino acid substitutions compared with the mcr-3 A novel genetic structure, IS Kpn40-mcr-3-dgkA - IS Kpn40 , might be the key element mediating translocation of mcr-3 through the formation of circular form. The mcr-1 and mcr-3 genes co-located on a plasmid might pose huge threat to public health...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29758020/defining-icr-mo-an-intrinsic-colistin-resistance-determinant-from-moraxella-osloensis
#5
Wenhui Wei, Swaminath Srinivas, Jingxia Lin, Zichen Tang, Shihua Wang, Saif Ullah, Vishnu Goutham Kota, Youjun Feng
Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family...
May 14, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29755416/complete-genomic-analysis-of-a-salmonella-enterica-serovar-typhimurium-isolate-cultured-from-ready-to-eat-pork-in-china-carrying-one-large-plasmid-containing-mcr-1
#6
Wei Wang, Zulqarnain Baloch, Mingyuan Zou, Yinping Dong, Zixin Peng, Yujie Hu, Jin Xu, Nafeesa Yasmeen, Fengqin Li, Séamus Fanning
One mcr-1 -carrying ST34-type Salmonella Typhimurium WW012 was cultured from 3,200 ready-to-eat (RTE) pork samples in 2014 in China. Broth dilution method was applied to obtain the antimicrobial susceptibility of Salmonella Typhimurium WW012. Broth matting assays were carried out to detect transferability of this phenotype and whole-genome sequencing was performed to analyze its genomic characteristic. Thirty out of 3,200 RTE samples were positive for Salmonella and the three most frequent serotypes were identified as S ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29741287/longitudinal-study-on-the-occurrence-in-pigs-of-colistin-resistant-e-coli-carrying-mcr-1-following-the-cessation-of-use-of-colistin
#7
Luke P Randall, Robert A Horton, Fabrizio Lemma, Francesca Martelli, Nick A D Duggett, Richard P Smith, Miranda J Kirchner, Richard J Ellis, Jon P Rogers, Susanna M Williamson, Robin R L Simons, Camilla M Brena, Sarah J Evans, Muna F Anjum, Christopher J Teale
AIMS: In 2015, colistin-resistant Escherichia coli and Salmonella with the mcr-1 gene were isolated from a pig farm in Great Britain. Pigs were subsequently monitored over a ~ 20 month period for the occurrence of mcr-1 mediated colistin resistance and the risk of mcr-1 E. coli entering the food chain was assessed. METHODS AND RESULTS: Pig faeces and slurry were cultured for colistin resistant E. coli and Salmonella, tested for the mcr-1 gene by PCR, and selected isolates were further analysed...
May 9, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29731740/evidence-for-environmental-dissemination-of-antibiotic-resistance-mediated-by-wild-birds
#8
Jiao Wu, Ye Huang, Dawei Rao, Yongkui Zhang, Kun Yang
The aquatic bird, egret, could carry antibiotic resistance (AR) from a contaminated waterway (Jin River, Chengdu, China) into the surrounding environment (Wangjianglou Park). A systematic study was carried out on the unique environmental dissemination mode of AR mediated by birds. The minimum inhibitory concentrations of various antibiotics against the environmental Escherichia coli isolates were used to evaluate the bacterial AR at the environmental locations where these isolates were recovered, i.e., the Jin River water, the egret feces, the park soil, and the campus soil...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29731646/in-vitro-antibacterial-effect-of-fosfomycin-combination-therapy-against-colistin-resistant-klebsiella-pneumoniae
#9
Wei Yu, Qixia Luo, Qingyi Shi, Chen Huang, Xiao Yu, Tianshui Niu, Kai Zhou, Jiajie Zhang, Yonghong Xiao
Objectives: Colistin is still a "last-resort" antibiotic used to manage human infections due to multidrug-resistant (MDR) Klebsiella pneumoniae . However, colistin-resistant K. pneumoniae (CR-Kp) isolates emerged a decade ago and had a worldwide distribution. The purpose of this study was to evaluate the genetic data of CR-Kp and identify the antibacterial activity of fosfomycin (FM) alone and in combination with amikacin (AMK) or colistin (COL) against CR-Kp in vitro. Methods: Three clinical CR-Kp isolates from three patients were collected...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29730781/role-of-antiplatelet-therapy-in-the-durability-of-hemodialysis-access
#10
Satinderjit Locham, Robert J Beaulieu, Hanaa Dakour-Aridi, Besma Nejim, Mahmoud B Malas
BACKGROUND: Antiplatelet therapy (APT) is often used on anecdotal grounds to improve vascular access patency. The aim of this study was to assess the role of APT in hemodialysis (HD) patients undergoing arteriovenous fistula (AVF) or graft (AVG) placement. METHODS: All patients in a large HD vascular qualitative initiative database (2011-2017) were included and divided into no antiplatelet therapy (no-APT) vs. any APT [aspirin (ASA) or P2Y12 inhibitors (PI)]. Multivariate [logistic (MLR) and Cox (MCR) regression] analyses were used as appropriate...
May 5, 2018: Journal of Nephrology
https://www.readbyqxmd.com/read/29729080/discovery-of-benzimidazole-based-l-mexicana-cysteine-protease-cpb2-8%C3%AE-cte-inhibitors-as-potential-therapeutics-for-leishmaniasis
#11
Laura De Luca, Stefania Ferro, Maria Rosa Buemi, Anna-Maria Monforte, Rosaria Gitto, Tanja Schirmeister, Louis Maes, Antonio Rescifina, Nicola Micale
Chemotherapy is currently the only effective approach to treat all forms of leishmaniasis. However, its effectiveness is severely limited due to high toxicity, long treatment length, drug-resistance or inadequate mode of administration. As a consequence, there is a need to identify new molecular scaffolds and targets as potential therapeutics for the treatment of this disease. We report a small series of 1,2-substituted-1H-benzo[d]imidazole derivatives (9a-d) showing affinity in the submicromolar range (Ki = 0...
May 4, 2018: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29721015/a-comparative-assessment-of-cardiovascular-autonomic-reflex-testing-and-cardiac-123-i-metaiodobenzylguanidine-imaging-in-patients-with-type-1-diabetes-mellitus-without-complications-or-cardiovascular-risk-factors
#12
Triantafyllos Didangelos, Efstratios Moralidis, Eleni Karlafti, Konstantinos Tziomalos, Charalambos Margaritidis, Zisis Kontoninas, Ioannis Stergiou, Maria Boulbou, Marianthi Papagianni, Emmanouel Papanastasiou, Apostolos I Hatzitolios
Aim: To compare the cardiovascular autonomic reflex tests (CARTs) with cardiac sympathetic innervation imaging with 123 I-metaiodobenzylguanidine (MIBG) in patients with type 1 diabetes mellitus (T1DM). Patients and Methods: Forty-nine patients (29 males, mean age 36 ± 10 years, mean T1DM duration 19 ± 6 years) without cardiovascular risk factors were prospectively enrolled. Participants were evaluated for autonomic dysfunction by assessing the mean circular resultant (MCR), Valsalva maneuver (Vals), postural index (PI), and orthostatic hypotension (OH)...
2018: International Journal of Endocrinology
https://www.readbyqxmd.com/read/29718423/efflux-pump-inhibitor-cccp-to-rescue-colistin-susceptibility-in-mcr-1-plasmid-mediated-colistin-resistant-strains-and-gram-negative-bacteria
#13
Sophie A Baron, Jean-Marc Rolain
Objectives: Efflux in bacteria is a ubiquitous mechanism associated with resistance to antimicrobials agents. Efflux pump inhibitors (EPIs) have been developed to inhibit efflux mechanisms and could be a good alternative to reverse colistin resistance, but only CCCP has shown good activity. The aim of our study was to identify CCCP activity in a collection of 93 Gram-negative bacteria with known and unknown colistin resistance mechanisms including isolates with mcr-1 plasmid-mediated colistin resistance...
April 26, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29718144/genomic-surveillance-reveals-diversity-of-multidrug-resistant-organism-colonization-and-infection-a-prospective-cohort-study-in-liver-transplant-recipients
#14
Nenad Macesic, Angela Gomez-Simmonds, Sean B Sullivan, Marla J Giddins, Samantha A Ferguson, Gautam Korakavi, David Leeds, Sarah Park, Kevin Shim, Madeleine G Sowash, Melanie Hofbauer, Ryan Finkel, Yue Hu, Jared West, Nora C Toussaint, William G Greendyke, Benjamin A Miko, Marcus R Pereira, Susan Whittier, Elizabeth C Verna, Anne-Catrin Uhlemann
Background: Multidrug-resistant organisms (MDROs) are an important cause of morbidity and mortality after solid organ transplantation. We aimed to characterize MDRO colonization dynamics and infection in liver transplant (LT) recipients through innovative use of active surveillance and whole-genome sequencing (WGS). Methods: We prospectively enrolled consecutive adult patients undergoing LT from March 2014 to March 2016. Fecal samples were collected at multiple timepoints from time of enrollment to 12 months posttransplant...
April 28, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29716921/a-multi-center-phase-1-trial-of-ipilimumab-in-myelodysplastic-syndrome-patients-following-hypomethylating-agent-failure
#15
Amer M Zeidan, Hanna A Knaus, Tara M Robinson, Andrea Mh Towlerton, Edus H Warren, Joshua F Zeidner, Amanda L Blackford, Amy S Duffield, David Rizzieri, Mark G Frattini, Yair M Levy, Mark A Schroeder, Anna Ferguson, Katherine E Sheldon, Amy E DeZern, Ivana Gojo, Steven D Gore, Howard Streicher, Leo Luznik, B Douglas Smith
PURPOSE: After failure of hypomethylating agents (HMAs), patients with myelodysplastic syndromes (MDS) have dismal survival and no approved treatment options. EXPERIMENTAL DESIGN: We conducted a phase 1b investigator-initiated trial of ipilimumab in patients with high-risk MDS that have failed HMAs. Patients received monotherapy at 2 dose-levels (DL; 3 and 10mg/kg) with an induction and a maintenance phase. Toxicities and responses were evaluated with CTCAE.4 and IWG-2006 criteria...
May 1, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29712655/genetic-and-functional-characterization-of-an-mcr-3-like-producing-escherichia-coli-recovered-from-swine-brazil
#16
Nicolas Kieffer, Patrice Nordmann, Andrea Micke Moreno, Luisa Zanolli Moreno, Richard Chaby, Aude Breton, Pierre Tissières, Laurent Poirel
A collection of 126 pigs were screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eigth colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State, producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a similar function as MCR-1 and MCR-2 by adding a phosphoethanolamine group to the lipid A. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C2 plasmid and was embedded in an original genetic environment...
April 30, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29708238/quantitative-analysis-of-multiple-high-resolution-mass-spectrometry-images-using-chemometric-methods-quantitation-of-chlordecone-in-mouse-liver
#17
Saeedeh Mohammadi, Hadi Parastar
In this work, a chemometrics-based strategy is developed for quantitative mass spectrometry imaging (MSI). In this regard, quantification of chlordecone as a carcinogenic organochlorinated pesticide (C10Cll0O) in mouse liver using the matrix-assisted laser desorption ionization MSI (MALDI-MSI) method is used as a case study. The MSI datasets corresponded to 1, 5 and 10 days of mouse exposure to the standard chlordecone in the quantity range of 0 to 450 μg g-1. The binning approach in the m/z direction is used to group high resolution m/z values and to reduce the big data size...
April 30, 2018: Analyst
https://www.readbyqxmd.com/read/29696722/the-first-human-report-of-mobile-colistin-resistance-gene-mcr-1-in-finland
#18
Kirsi Gröndahl-Yli-Hannuksela, Emilia Lönnqvist, Teemu Kallonen, Laura Lindholm, Jari Jalava, Kaisu Rantakokko-Jalava, Jaana Vuopio
Colistin resistance mediated by mobile mcr-1 gene has raised concern during the last years. After steep increase in mcr-1 reports, other mcr-gene variants (mcr-2 to mcr-5) have been revealed as well. In 2016, a clinical study was conducted on asymptomatic stool carriage of extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae among Finnish adults. All suspected ESBL producing bacterial isolates were first tested by phenotypic ESBL-confirmation methods, and then further analyzed with whole genome sequencing to identify the resistance genes...
May 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29688801/genomic-characterization-of-nonclonal-mcr-1-positive-multidrug-resistant-klebsiella-pneumoniae-from-clinical-samples-in-thailand
#19
Apichai Srijan, Katie R Margulieux, Sirigade Ruekit, Erik Snesrud, Rosslyn Maybank, Oralak Serichantalergs, Rosarin Kormanee, Prawet Sukhchat, Jossin Sriyabhaya, Mary Hinkle, John M Crawford, Patrick McGann, Brett E Swierczewski
Multidrug-resistant Klebsiella pneumoniae strains are one of the most prevalent causes of nosocomial infections and pose an increasingly dangerous public health threat. The lack of remaining treatment options has resulted in the utilization of older drug classes, including colistin. As a drug of last resort, the discovery of plasmid-mediated colistin resistance by mcr-1 denotes the potential development of pandrug-resistant bacterial pathogens. To address the emergence of the mcr-1 gene, 118 gram-negative Enterobacteriaceae isolated from clinical samples collected at Queen Sirikit Naval Hospital in Chonburi, Thailand were screened for colistin resistance using automated antimicrobial susceptibility testing and conventional PCR screening...
April 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29686149/retained-activity-of-an-o25b-specific-monoclonal-antibody-against-mcr-1-producing-escherichia-coli-st131
#20
Luis M Guachalla, Katharina Ramoni, Cecilia Varga, Michele Mutti, Akela Ghazawi, Tibor Pál, Eszter Nagy, Ágnes Sonnevend, Gábor Nagy, Valéria Szijártó
Plasmid-encoded colistin resistance is emerging among extra-intestinal pathogenic Escherichia coli including the epidemic clone ST131-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of LPS conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody, ASN-4 specific to the O25b side-chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin neutralizing activity and therefore offer a treatment option against extremely drug-resistant ST131 isolates...
April 23, 2018: Antimicrobial Agents and Chemotherapy
keyword
keyword
115766
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"