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https://www.readbyqxmd.com/read/27273983/-application-of-tendon-derived-stem-cells-and-bone-marrow-derived-mesenchymal-stem-cells-for-tendon-injury-repair-in-rat-model
#1
Xiangpeng Kong, Ming Ni, Guoqiang Zhang, Wei Chai, Xiang Li, Yucong Li, Yan Wang
OBJECTIVE: To evaluate the application of tendon-derived stem cells (TDSC) and bone marrow-derived mesenchymal stem cells (BMSC) for patellar tendon injury repair in rat model. METHODS: TDSCs and BMSCs were isolated from patellar tendons or bone marrow of healthy SD rats. The patellar tendon injury model was induced in 60 SD rats, then the animals were divided into 3 groups with 20 in each group: rats in TDSC group received transplantation of TDSC with fibrin glue in defected patellar tendon, rats in BMSC group received BMSC with fibrin glue for transplantation and those in control group received fibrin glue only...
March 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/27130678/isolation-and-biological-characterization-of-tendon-derived-stem-cells-from-fetal-bovine
#2
Jinjuan Yang, Qianjun Zhao, Kunfu Wang, Hao Liu, Caiyun Ma, Hongmei Huang, Yingjie Liu
The lack of appropriate candidates of cell sources for cell transplantation has hampered efforts to develop therapies for tendon injuries, such as tendon rupture, tendonitis, and tendinopathy. Tendon-derived stem cells (TDSCs) are a type of stem cells which may be used in the treatment of tendon injuries. In this study, TDSCs were isolated from 5-mo-old Luxi Yellow fetal bovine and cultured in vitro and further analyzed for their biological characteristics using immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) assays...
September 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27100314/can-transcranial-direct-current-stimulation-improve-the-resistance-strength-and-decrease-the-rating-perceived-scale-in-recreational-weight-training-experience
#3
Eduardo Lattari, Maria L Andrade, Alberto S Filho, Antônio M Moura, Geraldo M Neto, Júlio G Silva, Nuno B Rocha, Ti-Fei Yuan, Oscar Arias-Carrión, Sérgio Machado
Lattari, E, Andrade, ML, Filho, AS, Moura, AM, Neto, GM, Silva, JG, Rocha, NB, Yuan, T-F, Arias-Carrión, O, and Machado, S. Can transcranial direct current stimulation improve the resistance strength and decrease the rating perceived scale in recreational weight-training experience? J Strength Cond Res 30(12): 3381-3387, 2016-The goal of this study was to evaluate the acute efficacy of anodic transcranial direct current stimulation on the total volume of repetitions and perceived exertion in recreationally trained individuals in strength...
December 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/26794903/tendon-derived-stem-cells-undergo-spontaneous-tenogenic-differentiation
#4
Jia Guo, Kai-Ming Chan, Jin-Fang Zhang, Gang Li
Tendon-derived stem cell (TDSC) is a subpopulation of residing stem cells within the intact tendon tissues, with the capacities of self-renewal, clonogenicity, and three-lineage differentiation. Compared with bone marrow derived mesenchymal stem cells (BMSCs), TDSCs are superior for tendon injuries repair as they remain some tendon tissue-specific differentiation properties. In the present study, TDSC was found to undergo spontaneous tenogenic differentiation in which the expression of tenogenic markers were increased while the expression of stemness markers decreased with time in TDSCs culture (without tenogenic induction medium)...
February 1, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/26719200/transplantation-of-tendon-derived-stem-cells-pre-treated-with-connective-tissue-growth-factor-and-ascorbic-acid-in-vitro-promoted-better-tendon-repair-in-a-patellar-tendon-window-injury-rat-model
#5
Pauline Po Yee Lui, On Tik Wong, Yuk Wa Lee
BACKGROUND AIMS: Treatment of tendon-derived stem cells (TDSCs) with connective tissue growth factor (CTGF) and ascorbic acid promoted their tenogenic differentiation. We investigated the effects of TDSCs pre-treated with CTGF and ascorbic acid on tendon repair in a patellar tendon window injury rat model. METHODS: Green fluorescent protein-TDSCs (GFP-TDSCs) were pre-treated with or without CTGF and ascorbic acid for 2 weeks before transplantation. The patellar tendons of rats were injured and divided into three groups: fibrin glue-only group (control group), untreated and treated TDSC group...
January 2016: Cytotherapy
https://www.readbyqxmd.com/read/26666429/purification-and-characterization-of-a-novel-chitinase-from-trichosanthes-dioica-seed-with-antifungal-activity
#6
Syed Rashel Kabir, Md Musfikur Rahman, Shahnima Tasnim, Md Rezaul Karim, Nazma Khatun, Imtiaj Hasan, Ruhul Amin, Shaikh Shohidul Islam, Md Nurujjaman, Ahmad Humayan Kabir, Niranjan Kumar Sana, Yasuhiro Ozeki, A K M Asaduzzaman
Chitinases are a group of enzymes that show differences in their molecular structure, substrate specificity, and catalytic mechanism and widely found in organisms like bacteria, yeasts, fungi, arthropods actinomycetes, plants and humans. A novel chitinase enzyme (designated as TDSC) was purified from Trichosanthes dioica seed with a molecular mass of 39±1 kDa in the presence and absence of β-mercaptoethanol. The enzyme was a glycoprotein in nature containing 8% neutral sugar. The N-terminal sequence was determined to be EINGGGA which did not match with other proteins...
March 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26599628/tgif1-gene-silencing-in-tendon-derived-stem-cells-improves-the-tendon-to-bone-insertion-site-regeneration
#7
Liyang Chen, Chaoyin Jiang, Shashi Ranjan Tiwari, Amrit Shrestha, Pengcheng Xu, Wenqing Liang, Yeqing Sun, Shisheng He, Biao Cheng
BACKGROUND/AIMS: The slow healing process of tendon-to-bone junctions can be accelerated via implanted tendon-derived stem cells (TDSCs) with silenced transforming growth interacting factor 1 (TGIF1) gene. Tendon-to-bone insertion site is the special form of connective tissues derivatives of common connective progenitors, where TGF-β plays bidirectional effects (chondrogenic or fibrogenic) through different signaling pathways at different stages. A recent study revealed that TGF-β directly induces the chondrogenic gene Sox9...
2015: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/26510918/comparing-the-osteogenic-potential-of-bone-marrow-and-tendon-derived-stromal-cells-to-repair-a-critical-sized-defect-in-the-rat-femur
#8
Nadja Kunkel, Andrea Wagner, Renate Gehwolf, Patrick Heimel, Herbert Tempfer, Stefanie Korntner, Peter Augat, Herbert Resch, Heinz Redl, Oliver Betz, Hans-Christian Bauer, Andreas Traweger
Despite significant advancements in bone tissue-engineering applications, the clinical impact of bone marrow stromal cells (BMSCs) for the treatment of large osseous defects remains limited. Therefore, other cell sources are under investigation for their osteogenic potential to repair bone. In this study, tendon-derived stromal cells (TDSCs) were evaluated in comparison to BMSCs to support the functional repair of a 5 mm critical-sized, segmental defect in the rat femur. Analysis of the trilineage differentiation capacity of TDSCs and BMSCs cultured on collagen sponges revealed impaired osteogenic differentiation and mineral deposition of TDSCs in vitro, whereas chondrogenic and adipogenic differentiation was evident for both cell types...
October 29, 2015: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/26229962/cyclic-tensile-strain-induces-tenogenic-differentiation-of-tendon-derived-stem-cells-in-bioreactor-culture
#9
Yuan Xu, Qiang Wang, Yudong Li, Yibo Gan, Pei Li, Songtao Li, Yue Zhou, Qiang Zhou
Different loading regimens of cyclic tensile strain impose different effects on cell proliferation and tenogenic differentiation of TDSCs in three-dimensional (3D) culture in vitro, which has been little reported in previous literatures. In this study we assessed the efficacy of TDSCs in a poly(L-lactide-co-ε-caprolactone)/collagen (P(LLA-CL)/Col) scaffold under mechanical stimulation in the custom-designed 3D tensile bioreactor, which revealed that cyclic tensile strain with different frequencies (0.3 Hz, 0...
2015: BioMed Research International
https://www.readbyqxmd.com/read/26133408/photoelectron-circular-dichroism-in-the-multiphoton-ionization-by-short-laser-pulses-i-propagation-of-single-active-electron-wave-packets-in-chiral-pseudo-potentials
#10
Anton N Artemyev, Anne D Müller, David Hochstuhl, Philipp V Demekhin
A theoretical method to study the angle-resolved multiphoton ionization of polyatomic molecules is developed. It is based on the time-dependent formulation of the Single Center (TDSC) method and consists in the propagation of single-active-electron wave packets in the effective molecular potentials in the presence of intense laser pulses. For this purpose, the time-dependent Schrödinger equation for one electron, moving in a molecular field and interacting with an arbitrary laser pulse, is solved in spherical coordinates by an efficient numerical approach...
June 28, 2015: Journal of Chemical Physics
https://www.readbyqxmd.com/read/25758330/platelet-rich-plasma-activates-tendon-derived-stem-cells-to-promote-regeneration-of-achilles-tendon-rupture-in-rats
#11
Kang Xu, Mohanad Kh Al-Ani, Yanjun Sun, Wei Xu, Lianhong Pan, Yang Song, ZhiLing Xu, Xin Pan, Li Yang
This study investigates whether platelet-rich plasma (PRP) is an activator of tendon-derived stem cells (TDSCs) to promote regeneration of Achilles tendon post-rupture in rats. In the in vitro study, PRGF (activated PRP) significantly enhanced cell DNA synthesis, improved viability and promoted proliferation, while facilitating cell migration and the recruitment of TDSCs. In addition, TDSCs were mixed with collagen and PRP to form collagen-TDSC constructs (CTC) and PRP-collagen-TDSC constructs (PCTCs). After 3 weeks of culture in vitro, we found that most of the encapsulated TDSCs in the CTCs and PCTCs were still alive, while cells in the PCTCs showed a more aligned arrangement compared to the CTCs...
March 11, 2015: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/25562162/tendon-derived-stem-cells-promote-platelet-rich-plasma-healing-in-collagenase-induced-rat-achilles-tendinopathy
#12
Lei Chen, Jun-Peng Liu, Kang-Lai Tang, Qing Wang, Guo-Dong Wang, Xian-Hua Cai, Xi-Ming Liu
BACKGROUND/AIMS: Tendon injuries are common, difficult to cure and usually healed with fibrosis and scar tissue. The aim of this study was to evaluate tendon derived stem cells (TDSCs) and platelet rich plasma (PRP) in the treatment of collagenase induced Achilles tendinopathy in rat. METHODS: Four and 8 weeks (n=18) after TDSCs, PRP, PRP with TDSC or PBS (control) injection into collagenase or saline (sham) injected rat Achilles tendon, tendon tissue was harvested and tendon quality was evaluated by histology and biomechanical testing...
2014: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/25297644/cdc-kerala-16-early-detection-of-developmental-delay-disability-among-children-below-6-y-a-district-model
#13
M K C Nair, G S Harikumaran Nair, M Beena, P Princly, S Abhiram Chandran, Babu George, M L Leena, Paul Swamidhas Sudhakar Russell
OBJECTIVE: To develop a district model for establishing early detection of childhood disability below 6 y of age and to develop appropriate referral linkages for confirmation of the diagnosis and establish home based early intervention therapy to all needy children. METHODS: Trained Accredited Social Health Activist (ASHA) workers conducted the preliminary survey for identifying developmental delay/disability among children below 6 y of age using Trivandrum Developmental Screening Chart (TDSC) (0-6 y) and a team of experts assessed the screen positives in developmental evaluation camps conducted at primary health centres (PHCs)...
December 2014: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/25294730/cdc-kerala-17-early-detection-of-developmental-delay-disability-among-children-below-3-y-in-kerala-a-cross-sectional-survey
#14
M K C Nair, P Princly, M L Leena, S Swapna, Lali Kumari I, R Preethi, Babu George, Paul Swamidhas Sudhakar Russell
OBJECTIVE: To conduct a community survey to understand the prevalence and type of developmental delay/disability among a representative state wide community sample of children below 3 y. METHODS: A state wide community based cross sectional survey was done with the help of the Integrated Child Development Services (ICDS) network in Kerala after giving one day training program at Child Development Centre (CDC), for one randomly selected anganwadi worker in each of the panchayath/municipal ward, from all districts of Kerala, to equip them to screen all children below 3 y in their anganwadi areas, using simple community screening tools like Trivandrum Developmental Screening Chart (TDSC) 0-3 and Language Evaluation Scale Trivandrum (LEST) 0-3...
December 2014: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/25186565/cdc-kerala-4-tdsc-items-based-developmental-therapy-package-among-low-birth-weight-babies-outcome-at-18-months-using-dasii
#15
M K C Nair, Rajee Krishnan, G S Harikumaran Nair, Deepa Bhaskaran, M L Leena, Babu George, Paul Swamidhas Sudhakar Russell
OBJECTIVE: To assess the effectiveness of Trivandrum Developmental Screening Chart (TDSC) items based intervention package developed at Child Development Centre, Kerala on the developmental outcome of children at 18 mo of age using Developmental Assessment Scale for Indian Infants (DASII) and compare the same in different birth weight groups. METHODS: Five hundred consecutive discharges from the Neonatal Intensive Care Unit (NICU), Sree Avittam Thirunal hospital, were recruited and followed up till 18 mo of age including 240 low birth weight (LBW;<2,500 g) babies and 260 normal birth weight babies...
December 2014: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/25124328/cdc-kerala-5-developmental-therapy-clinic-experience-use-of-child-development-centre-grading-for-motor-milestones
#16
M K C Nair, V R Resmi, Rajee Krishnan, G S Harikumaran Nair, M L Leena, Deepa Bhaskaran, Babu George, Paul Swamidhas Sudhakar Russell
OBJECTIVES: To document the experiences of the intervention given to children who attended the developmental therapy clinic of Child Development Centre (CDC) Kerala, a specialized clinic for providing developmental intervention/therapy for babies less than two years with developmental delay/disability. METHODS: All the babies referred to this speciality clinic from developmental screening/evaluation clinics of CDC were registered in the clinic and re-evaluation was done using CDC grading for head holding, sitting, standing, Amiel Tison passive angles, and Trivandrum Developmental Screening Chart (TDSC) 0-2 y...
December 2014: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/25038747/a-practical-guide-for-the-isolation-and-maintenance-of-stem-cells-from-tendon
#17
Pauline Po Yee Lui
Stem cells are unspecialized cells that can self-renew and have the ability to develop into cells of highly specialized functions. The study of stem cells holds enormous promise in the medical field ranging from their uses in cell therapies to their uses for greater understanding of tissue development and disease pathologies. Stem cells have been isolated from tendon tissue recently. These tendon-derived stem cells (TDSCs) are particularly relevant for tendon repair and the study of the potential roles of stem cells in tendon pathology as they are isolated from tendon tissues...
2015: Methods in Molecular Biology
https://www.readbyqxmd.com/read/24831949/scx-transduced-tendon-derived-stem-cells-tdscs-promoted-better-tendon-repair-compared-to-mock-transduced-cells-in-a-rat-patellar-tendon-window-injury-model
#18
Chunlai Tan, Pauline Po Yee Lui, Yuk Wa Lee, Yin Mei Wong
We hypothesized that the transplantation of Scx-transduced tendon-derived stem cells (TDSCs) promoted better tendon repair compared to the transplantation of mock-transduced cells. This study thus aimed to investigate the effect of Scx transduction on the expression of lineage markers in TDSCs and the effect of the resulting cell line in the promotion of tendon repair. Rat non-GFP or GFP-TDSCs were transduced with Scx or empty lentiviral vector (Mock) and selected by blasticidin. The mRNA expressions of Scx and different lineage markers were examined by qRT-PCR...
2014: PloS One
https://www.readbyqxmd.com/read/24798058/allogeneic-tendon-derived-stem-cells-promote-tendon-healing-and-suppress-immunoreactions-in-hosts-in-vivo-model
#19
Pauline Po Yee Lui, Siu Kai Kong, Pui Man Lau, Yin Mei Wong, Yuk Wa Lee, Chunlai Tan, On Tik Wong
The medium- to long-term healing effect and infiltration of inflammatory cells, after transplantation of allogeneic tendon-derived stem cell (TDSC) to the rat patellar tendon window wound, were examined. Allogeneic patellar TDSCs derived from a green fluorescent protein rat were used. The outcome of tendon healing and the infiltration of inflammatory cells were examined by histology and immunohistochemistry up to week 16 postinjury. The fate of the transplanted cells was examined by ex vivo fluorescent imaging and immunohistochemistry...
November 2014: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/24451112/application-of-tendon-derived-stem-cell-sheet-for-the-promotion-of-graft-healing-in-anterior-cruciate-ligament-reconstruction
#20
Pauline Po Yee Lui, On Tik Wong, Yuk Wa Lee
BACKGROUND: Both osteointegration and remodeling of graft midsubstance (collectively called graft healing) are slow processes after anterior cruciate ligament (ACL) reconstruction. Tendon-derived stem cells (TDSCs) form a cell sheet after treatment with connective tissue growth factor (CTGF) and ascorbic acid, which exhibits higher tenogenic and maintains high chondro-osteogenic gene expression of TDSCs. No external scaffold is required for cell delivery. HYPOTHESIS: Wrapping the TDSC sheet around the ACL graft would promote early graft healing in a rat model...
March 2014: American Journal of Sports Medicine
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