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https://www.readbyqxmd.com/read/28808748/erratum-to-characterization-of-three-pathway-specific-regulators-for-high-production-of-monensin-in-streptomyces-cinnamonensis
#1
Zheng-Kun Tang, Xiao-Mei Li, Ai-Ping Pang, Chun-Yan Lin, Yue Zhang, Jie Zhang, Jianjun Qiao, Guang-Rong Zhao
No abstract text is available yet for this article.
August 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28805884/the-effects-of-monensin-in-diets-fed-to-finishing-beef-steers-and-heifers-on-growth-performance-and-fecal-shedding-of-escherichia-coli-o157-h7
#2
K E Hales, J E Wells, E D Berry, N Kalchayanand, J L Bono, M Kim
Two experiments were conducted to study the effects of monensin dose on growth performance and O157:H7 shedding in finishing beef cattle. In Exp. 1, 198 heifers (298 ± 1.1 kg BW) were allocated to 1 of 2 treatments consisting of 1) 200 mg/heifer daily of monensin and 2) 400 mg/heifer daily of monensin and fed for 151 d. In Exp. 2, 199 steers (430 ± 1.9 kg BW) were stratified by BW and allocated to 1 of 2 treatments consisting of 1) 0 mg/steer daily of monensin and 2) 400 mg/steer daily of monensin and fed for 128 d...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805883/a-novel-objective-chute-score-interacts-with-monensin-to-affect-growth-of-receiving-cattle
#3
K A Bruno, E S Vanzant, A W Altman, M Kudupoje, K A Vanzant, K R McLeod
Growth in cattle may be related to animal temperament via alterations in intake or feed conversion. However, temperament is ill-defined, and different temperament measures may relate differently to production traits or interact with dietary factors in their effects. To examine relationships between diet, temperament, growth, and health, 160 crossbred steers (262 ± 22 kg) were used in a 56-d RCBD experiment with a 2 × 2 × 2 factorial treatment structure with 5 pens/treatment. Steers were pen fed a corn silage-based diet with or without monensin (41...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28792977/determination-of-regulatory-ionophore-coccidiostat-residues-in-feedstuffs-at-carry-over-levels-by-liquid-chromatography-mass-spectrometry
#4
Loredana Annunziata, Pierina Visciano, Arianna Stramenga, Maria Novella Colagrande, Guido Campana, Giampiero Scortichini, Giacomo Migliorati, Dario Compagnone
In this study samples of feedstuffs were collected from different feed mills and animal farms located in central Italy and analyzed for ionophore coccidiostat residues at carry-over levels by liquid chromatography-mass spectrometry. Since unavoidable cross-contamination of feedstuffs may occur during their production as well as distribution and storage, the collection of samples covered all these different stages. Residues of lasalocid, monensin, salinomycin and maduramicin were detected in 32.4% of samples, both at production and storage level...
2017: PloS One
https://www.readbyqxmd.com/read/28791638/activation-of-endosome-associated-inert-egf-receptor-following-internalization
#5
Yi Wang, Sukhmani Billing, Zhixiang Wang
Our current understanding of endosomal signaling is incomplete given our inability to generate endosomal signals in isolation from plasma membrane signals. We present a novel method to specifically activate epidermal growth factor (EGF) receptor (EGFR) following its internalization into endosomes. This method will allow us to address questions such as whether endosomal signaling is sufficient to activate signal transduction pathways and produce biological responses. In this method, the cells are treated with EGF in the presence of AG1478, a specific EGFR tyrosine kinase inhibitor, and monensin, which blocks recycling of EGFR...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28727060/effect-of-monensin-inclusion-on-intake-digestion-and-ruminal-fermentation-parameters-by-bos-taurus-indicus-and-bos-taurus-taurus-steers-consuming-bermudagrass-hay
#6
N L Bell, R C Anderson, T R Callaway, M O Franco, J E Sawyer, T A Wickersham
Effects of monensin inclusion and cattle subspecies on utilization of bermudagrass hay (13.7% CP, 77.3% NDF, and 38.8% ADF) were evaluated using ruminally cannulated steers (5 [BI] and 5 [BT]; 398 kg BW). Subspecies were concurrently subjected to a 2-period, 2-treatment crossover design. Treatments were 0 (CON) or 200 mg·steer·d monensin (MON) in 0.91 kg dried distillers' grains with solubles. Periods were 70 d in length: 20 d of adaptation, 22 d of sample collection, and 28 d for withdrawal of treatment...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727043/effect-of-monensin-withdrawal-on-intake-digestion-and-ruminal-fermentation-parameters-by-bos-taurus-indicus-and-bos-taurus-taurus-steers-consuming-bermudagrass-hay
#7
N L Bell, T R Callaway, R C Anderson, M O Franco, J E Sawyer, T A Wickersham
Effects of monensin withdrawal and cattle subspecies on the utilization of bermudagrass hay (14.3% CP, 72.3% NDF, and 36.9% ADF) were evaluated using ruminally cannulated steers (5 [BI] and 5 [BT]). Subspecies were concurrently subjected to a 2-period, 2-treatment crossover design. Treatments consisted of either 0 mg·steer·d-1 monensin with no previous monensin feeding (CON) or withdrawal from 200 mg·steer·d-1 monensin (MON) fed individually in 0.91 kg dried distillers' grains with solubles for 42 d. Withdrawal was evaluated for a 28-d period...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727009/effects-of-energy-supplementation-on-energy-losses-and-nitrogen-balance-of-steers-fed-green-chopped-wheat-pasture-i-calorimetry
#8
A L Shreck, P J Ebert, E A Bailey, J S Jennings, K D Casey, B E Meyer, N A Cole
Cattle grazing wheat pasture in the southern Great Plains are sometimes fed an energy supplement; however, the benefits of supplementation on nutrient balance, energy metabolism, and greenhouse gas emissions have not been elucidated. Therefore, we used 10 British crossbred steers (206 ± 10.7 kg initial BW) in a respiration calorimetry study to evaluate the effects of energy supplementation on energy losses, N balance, and nutrient digestibility of steers fed green-chopped wheat forage. The study design was an incomplete replicated 4 × 4 Latin square with treatments in a 2 × 2 factorial arrangement...
May 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28685195/characterization-of-three-pathway-specific-regulators-for-high-production-of-monensin-in-streptomyces-cinnamonensis
#9
Zheng-Kun Tang, Xiao-Mei Li, Ai-Ping Pang, Chun-Yan Lin, Yue Zhang, Jie Zhang, Jianjun Qiao, Guang-Rong Zhao
Monensin, a polyether ionophore antibiotic, is produced by Streptomyces cinnamonensis and worldwide used as a coccidiostat and growth-promoting agent in the field of animal feeding. The monensin biosynthetic gene cluster (mon) has been reported. In this study, the potential functions of three putatively pathway-specific regulators (MonH, MonRI, and MonRII) were clarified. The results from gene inactivation, complementation, and overexpression showed that MonH, MonRI, and MonRII positively regulate monensin production...
August 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28670304/monensin-and-nisin-affect-rumen-fermentation-and-microbiota-differently-in-vitro
#10
Junshi Shen, Zhuang Liu, Zhongtang Yu, Weiyun Zhu
Nisin, a bacteriocin, is a potential alternative to antibiotics to modulate rumen fermentation. However, little is known about its impacts on rumen microbes. This study evaluated the effects of nisin (1 and 5 μM) on in vitro rumen fermentation characteristics, microbiota, and select groups of rumen microbes in comparison with monensin (5 μM), one of the most commonly used ionophores in ruminants. Nisin had greater effects than monensin in inhibiting methane production and decreasing acetate/propionate ratio...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28665718/relationship-between-broiler-body-weights-eimeria-maxima-gross-lesion-scores-and-microscores-in-three-anticoccidial-sensitivity-tests
#11
Miguel A Barrios, Manuel Da Costa, Emily Kimminau, Lorraine Fuller, Steven Clark, Gene Pesti, Robert Beckstead
Anticoccidial sensitivity tests (ASTs) serve to determine the efficacy of anticoccidial drugs against Eimeria field isolates in a controlled laboratory setting. The most commonly measured parameters are body weight gain, feed conversion ratio, gross intestinal lesion scores, and mortality. Due to the difficulty in reliably scoring gross lesion scores of Eimeria maxima , microscopic analysis of intestinal scrapings (microscores) can be used in the field to indicate the presence of this particular Eimeria. The goal of this study was to determine the relationship between E...
June 2017: Avian Diseases
https://www.readbyqxmd.com/read/28557191/effect-of-increasing-monensin-sodium-levels-in-diets-with-virginiamycin-on-the-finishing-of-nellore-cattle
#12
João Marcos B Benatti, João Alexandrino Alves Neto, Ivanna M de Oliveira, Flávio D de Resende, Gustavo R Siqueira
This study evaluated the effect of increasing levels of monensin sodium (MON) in diets with virginiamycin (VM) on the finishing of feedlot cattle. Two hundred and eighty intact male Nellore cattle (348 ± 32 kg body weight, 22 months) received one of the following five diets: control diet (without additives); diet containing VM (25 mg per kg dry matter) combined with 0 (MON0), 10 (MON10), 20 (MON20) or 30 (MON30) mg MON per kg dry matter. During adaptation (28 days), the MON0 diet increased dietary net energy for maintenance and gain compared to the control diet (P = 0...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28549691/27-hydroxycholesterol-upregulates-the-production-of-heat-shock-protein-60-of-monocytic-cells
#13
Bo-Young Kim, Yonghae Son, Jeongyoon Choi, Seong-Kug Eo, Young Chul Park, Koanhoi Kim
Investigating differentially expressed proteins in a milieu rich in cholesterol oxidation products, we found via mass spectrometry-based proteomics that surface levels of heat shock protein 60 (HSP60) were upregulated on monocytic cells in the presence of 27-hydroxycholesterol (27OHChol). The elevated levels of cytoplasmic membrane HSP60 were verified via Western blot analysis and visualized by confocal microscopy. Treatment with 27OHChol also resulted in increased levels of cellular HSP60 without altering its transcription...
September 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28516409/survival-after-severe-rhabdomyolysis-following-monensin-ingestion
#14
Michela Blain, Alexander Garrard, Robert Poppenga, Betty Chen, Matthew Valento, Melissa Halliday Gittinger
INTRODUCTION: Monensin is a veterinary antibiotic with a narrow therapeutic window that has led to lethal intoxication in many animal species. Only two prior cases of human toxicity have been reported, both fatal. We present the first case of survival from severe toxicity following monensin ingestion. CASE: A 58-year-old man presented with 8 days of vomiting and abdominal pain. Due to delusions of central nervous system toxoplasmosis, he ingested 300 mg of monensin...
May 17, 2017: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/28492364/na-influx-via-orai1-inhibits-intracellular-atp-induced-mtorc2-signaling-to-disrupt-cd4-t-cell-gene-expression-and-differentiation
#15
Yong Miao, Jaya Bhushan, Adish Dani, Monika Vig
T cell effector functions require sustained calcium influx. However, the signaling and phenotypic consequences of non-specific sodium permeation via calcium channels remain unknown. α-SNAP is a crucial component of Orai1 channels, and its depletion disrupts the functional assembly of Orai1 multimers. Here we show that α-SNAP hypomorph, hydrocephalus with hopping gait, Napa(hyh/hyh) mice harbor significant defects in CD4 T cell gene expression and Foxp3 regulatory T cell (Treg) differentiation. Mechanistically, TCR stimulation induced rapid sodium influx in Napa(hyh/hyh) CD4 T cells, which reduced intracellular ATP, [ATP]i...
May 11, 2017: ELife
https://www.readbyqxmd.com/read/28476891/drosophila-tg-a-transglutaminase-is-secreted-via-an-unconventional-golgi-independent-mechanism-involving-exosomes-and-two-types-of-fatty-acylations
#16
Toshio Shibata, Jinki Hadano, Daichi Kawasaki, Xiaoqing Dong, Shun-Ichiro Kawabata
Transglutaminases (TGs) play essential intracellular and extracellular roles by covalently cross-linking many proteins. Drosophila TG is encoded by one gene and has two alternative splicing-derived isoforms, TG-A and TG-B, which contain distinct N-terminal 46- and 38-amino acid sequences, respectively. The TGs identified to date do not have a typical endoplasmic reticulum (ER)-signal peptide, and the molecular mechanisms of their secretion under physiologic conditions are unclear. Immunocytochemistry revealed that TG-A localizes to multivesicular-like structures, whereas TG-B localizes to the cytosol...
June 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28434981/protein-transport-inhibitors-downregulate-the-expression-of-lag-3-on-regulatory-t-cells
#17
Sara Anvari, Andrea Grimbergen, Carla M Davis, George Makedonas
Modern immunologic studies demand increasing complexity because of a need to improve our understanding of the relationship between a cell's phenotype and its function. Regulatory T cells (Tregs) have been defined by a narrow set of phenotypic markers, however their actual functional capacity has not been determined at the single-cell level. Although the lymphocyte activation gene 3 (LAG-3; CD223) is a key marker for the identification of exhausted T cells, it may be useful also in resolving Treg subpopulations by indicating distinct functional breadths...
April 21, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28402029/magnetic-solid-phase-extraction-based-on-carbon-nanotubes-for-the-determination-of-polyether-antibiotic-and-s-triazine-drug-residues-in-animal-food-with-lc-ms-ms
#18
Xiaoxing Liu, Shuyu Xie, Tengteng Ni, Dongmei Chen, Xu Wang, Yuanhu Pan, Yulian Wang, Lingli Huang, Guyue Cheng, Wei Qu, Zhenli Liu, Yanfei Tao, Zonghui Yuan
Carbon nanotubes-magnetic nanoparticles, comprising ferroferric oxide nanoparticles and carbon nanotubes, were prepared through a simple one-step synthesis method and subsequently applied to magnetic solid-phase extraction for the determination of polyether antibiotic and s-triazine drug residues in animal food coupled with liquid chromatography with tandem mass spectrometry. The nanocomposites were characterized by transmission electron microscopy, X-ray diffraction, and vibrating sample magnetometry. The components within the nanocomposites endowed the material with high extraction performance and manipulative convenience...
April 12, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28391864/a-simple-and-high-throughput-method-for-determination-and-confirmation-of-14-coccidiostats-in-poultry-muscle-and-eggs-using-liquid-chromatography-quadrupole-linear-ion-trap-tandem-mass-spectrometry-hplc-qqlit-ms-ms-validation-according-to-european-union-2002
#19
Fabiano Barreto, Cristina Ribeiro, Rodrigo Barcellos Hoff, Teresa Dalla Costa
A simple and fast quantitative and confirmatory multi-residue method was developed and validated for the determination of 14 coccidiostats residues in poultry muscle and eggs using liquid chromatography-tandem mass spectrometry (LC/MS-MS). The compounds were analyzed in a single run including lasalocid A, maduramicin, monensin, narasin, salinomycin, semduramicin, robenidine, diclazuril, toltrazuril, trimethoprim, clopidol, amprolium, diaveridine and nicarbazin (as the marker residue dinitrocarbanilide). A low-cost extraction and clean up procedure was optimized without the need of solid-phase extraction...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28386903/long-term-broiler-litter-amendments-can-alter-the-soil-s-capacity-to-sorb-monensin
#20
Sarah A Doydora, Peizhe Sun, Miguel Cabrera, Nehru Mantripragada, John Rema, Spyros G Pavlostathis, Ching-Hua Huang, Aaron Thompson
Monensin is a common antiparasitic drug given to poultry that contaminates poultry manure and bedding material (broiler litter). As broiler litter is commonly applied to agricultural fields as fertilizer, monensin could be released beyond the farm if it is not retained or degraded in the soil. This study aimed to assess the impact of long-term surface application of broiler litter (i.e., 17 years) on the capacity of pasture soil to sorb monensin. The soils were exposed to a range of monensin concentrations (0...
May 2017: Environmental Science and Pollution Research International
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