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Regenerative injection therapy

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https://www.readbyqxmd.com/read/29229249/enzyme-mediated-tissue-adhesive-hydrogels-for-meniscus-repair
#1
Su-Hwan Kim, Young-Hyeon An, Hwan D Kim, Kyungmin Kim, Sang-Hyuk Lee, Hyun-Gu Yim, Byung-Gee Kim, Nathaniel S Hwang
Meniscus tissues have limited regenerative capacity once damaged. The treatment options for the meniscus tissue regeneration have been limited to arthroscopic meniscectomy or surgical interventions. The injectable hydrogels based system would provide an alternative to the conventional meniscus therapy by providing a minimally invasive treatment alternative. Here we utilized enzyme-based approaches to fabricate tissue adhesive hydrogels for meniscus repair. Tyramine (TA) conjugated hyaluronic acid (TA-HA) and gelatin are susceptible to tyrosinase (TYR)-mediated crosslinking in vitro and in vivo...
December 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29226477/cell-therapy-for-critical-limb-ischemia-an-integrated-review-of-pre-clinical-and-clinical-studies
#2
REVIEW
Mohammad Qadura, Daniella C Terenzi, Subodh Verma, Mohammed Al-Omran, David A Hess
Critical limb ischemia (CLI), the most severe form of peripheral artery disease, is characterized by pain at rest and non-healing ulcers in the lower extremities. For patients with CLI, where the extent of atherosclerotic artery occlusion is too severe for surgical bypass or percutaneous interventions, limb amputation remains the only treatment option. Thus, cell-based therapy to restore perfusion and promote wound healing in patients with CLI is under intense investigation. Despite promising preclinical studies in animal models, transplantation of bone marrow (BM) derived cell populations in patients with CLI has shown limited benefit preventing limb amputation...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29201496/platelets-prime-hematopoietic-and-vascular-niche-to-drive-angiocrine-mediated-liver-regeneration
#3
Koji Shido, Deebly Chavez, Zhongwei Cao, Jane Ko, Shahin Rafii, Bi-Sen Ding
In mammals, the livers regenerate after chemical injury or resection of hepatic lobe by hepatectomy. How liver regeneration is initiated after mass loss remains to be defined. Here, we report that following liver injury, activated platelets deploy SDF-1 and VEGF-A to stimulate CXCR7+ liver sinusoidal endothelial cell (LSEC) and VEGFR1+ myeloid cell, orchestrating hepatic regeneration. After carbon tetrachloride (CCl4) injection or hepatectomy, platelets and CD11b+VEGFR1+ myeloid cells were recruited LSEC, and liver regeneration in both models was impaired in thrombopoietin-deficient (Thpo-/-) mice lacking circulating platelets...
2017: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29199133/innovative-strategies-for-intervertebral-disc-regenerative-medicine-from-cell-therapies-to-multiscale-delivery-systems
#4
REVIEW
Nina Henry, Johann Clouet, Jean Le Bideau, Catherine Le Visage, Jérôme Guicheux
As our understanding of the physiopathology of intervertebral disc (IVD) degeneration has improved, novel therapeutic strategies have emerged, based on the local injection of cells, bioactive molecules, and nucleic acids. However, with regard to the harsh environment constituted by degenerated IVDs, protecting biologics from in situ degradation while allowing their long-term delivery is a major challenge. Yet, the design of the optimal approach for IVD regeneration is still under debate and only a few papers provide a critical assessment of IVD-specific carriers for local and sustained delivery of biologics...
December 1, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/29196647/injectable-nanohydroxyapatite-chitosan-gelatin-micro-scaffolds-induce-regeneration-of-knee-subchondral-bone-lesions
#5
B Wang, W Liu, D Xing, R Li, C Lv, Y Li, X Yan, Y Ke, Y Xu, Y Du, J Lin
Subchondral bone has been identified as an attractive target for KOA. To determine whether a minimally invasive micro-scaffolds could be used to induce regeneration of knee subchondral bone lesions, and to examine the protective effect of subchondral bone regeneration on upper cartilage, a ready-to-use injectable treatment with nanohydroxyapatite-chitosan-gelatin micro-scaffolds (HaCGMs) is proposed. Human-infrapatellar-fat-pad-derived adipose stem cells (IPFP-ASCs) were used as a cellular model to examine the osteo-inductivity and biocompatibility of HaCGMs, which were feasibly obtained with potency for multi-potential differentiations...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29190645/cd105-mesenchymal-stem-cells-migrate-into-osteoarthritis-joint-an-animal-model
#6
Pablo Fernandez-Pernas, Iván Rodríguez-Lesende, Alexandre de la Fuente, Jesús Mateos, Isaac Fuentes, Javier De Toro, Fco J Blanco, M C Arufe
Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated the risk of xenogenicity of human synovial membrane-derived MSCs, injected into the monkeys using intravenous and intra-articular administration. The animal models used were adult monkeys Rhesus which had been injured into the left knee to create an Osteoarthritis (OA) animal model...
2017: PloS One
https://www.readbyqxmd.com/read/29188796/in-situ-regeneration-of-retinal-pigment-epithelium-by-gene-transfer-of-e2f2-a-potential-strategy-for-treatment-of-macular-degenerations
#7
D Kampik, M Basche, U F O Luhmann, K M Nishiguchi, J A E Williams, J Greenwood, S E Moss, H Han, S Azam, Y Duran, S J Robbie, J W B Bainbridge, D F Larkin, A J Smith, R R Ali
The retinal pigment epithelium (RPE) interacts closely with photoreceptors to maintain visual function. In degenerative diseases such as Stargardt disease and age-related macular degeneration, the leading cause of blindness in the developed world, RPE cell loss is followed by photoreceptor cell death. RPE cells can proliferate under certain conditions, suggesting an intrinsic regenerative potential, but so far this has not been utilised therapeutically. Here, we used E2F2 to induce RPE cell replication and thereby regeneration...
November 30, 2017: Gene Therapy
https://www.readbyqxmd.com/read/29179771/paracrine-effects-of-human-amniotic-epithelial-cells-protect-against-chemotherapy-induced-ovarian-damage
#8
Qiuwan Zhang, Shixia Bu, Junyan Sun, Minhua Xu, Xiaofen Yao, Kunyan He, Dongmei Lai
BACKGROUND: Human amniotic epithelial cells (hAECs) are attractive candidates for regenerative medical therapy, with the potential to replace deficient cells and improve functional recovery after injury. Previous studies have demonstrated that transplantation of hAECs effectively alleviate chemotherapy-induced ovarian damage via inhibiting granulose cells apoptosis in animal models of premature ovarian failure/insufficiency (POF/POI). However, the underlying molecular mechanism accounting for hAECs-mediated ovarian function recovery is not fully understood...
November 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29155703/modeling-amyloid-%C3%AE-42-toxicity-and-neurodegeneration-in-adult-zebrafish-brain
#9
Prabesh Bhattarai, Alvin Kuriakose Thomas, Mehmet Ilyas Cosacak, Christos Papadimitriou, Violeta Mashkaryan, Yixin Zhang, Caghan Kizil
Alzheimer's disease (AD) is a debilitating neurodegenerative disease in which accumulation of toxic amyloid-β42 (Aβ42) peptides leads to synaptic degeneration, inflammation, neuronal death, and learning deficits. Humans cannot regenerate lost neurons in the case of AD in part due to impaired proliferative capacity of the neural stem/progenitor cells (NSPCs) and reduced neurogenesis. Therefore, efficient regenerative therapies should also enhance the proliferation and neurogenic capacity of NSPCs. Zebrafish (Danio rerio) is a regenerative organism, and we can learn the basic molecular programs with which we could design therapeutic approaches to tackle AD...
October 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29145879/allogeneic-mesenchymal-stem-cell-as-induction-therapy-to-prevent-both-delayed-graft-function-and-acute-rejection-in-deceased-donor-renal-transplantation-study-protocol-for-a-randomized-controlled-trial
#10
Qipeng Sun, Liangqing Hong, Zhengyu Huang, Ning Na, Xuefeng Hua, Yanwen Peng, Ming Zhao, Ronghua Cao, Qiquan Sun
BACKGROUND: Using kidneys from deceased donors is an available strategy to meet the growing need of grafts. However, higher incidences of delayed graft function (DGF) and acute rejection exert adverse effects on graft outcomes. Since ischemia-reperfusion injury (IRI) and ongoing process of immune response to grafts are the major causes of DGF and acute rejection, the optimal induction intervention should possess capacities of both repairing renal structure injury and suppressing immune response simultaneously...
November 16, 2017: Trials
https://www.readbyqxmd.com/read/29141136/systemic-mrna-delivery-to-the-lungs-by-functional-polyester-based-carriers
#11
Yunfeng Yan, Hu Xiong, Xinyi Zhang, Qiang Cheng, Daniel John Siegwart
Messenger RNA (mRNA) has recently come into focus as an emerging therapeutic class with great potential for protein replacement therapy, cancer immunotherapy, regenerative medicine, vaccines, and gene editing. However, the lack of effective and safe delivery methods impedes the broad application of mRNA-based therapeutics. Here, we report a robust approach to develop efficient polymeric delivery carriers for mRNA. Lead polyesters were identified by in vitro screening of a 480-member combinatorially modified poly(trimethylolpropane allyl ether-co-suberoyl chloride) library for the delivery of luciferase encoding mRNA (Luc mRNA) to IGROV1 cells...
November 15, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29098296/manipulation-of-panx1-activity-increases-the-engraftment-of-transplanted-lacrimal-gland-epithelial-progenitor-cells
#12
Liana V Basova, Xin Tang, Takeshi Umasume, Anastasia Gromova, Tatiana Zyrianova, Taisia Shmushkovich, Alexey Wolfson, Dillon Hawley, Driss Zoukhri, Valery I Shestopalov, Helen P Makarenkova
Purpose: Sjögren's syndrome is a systemic chronic autoimmune inflammatory disease that primarily targets the salivary and lacrimal glands (LGs). Currently there is no cure; therefore, cell-based regenerative therapy may be a viable option. LG inflammation is facilitated by extracellular ATP and mediated by the Pannexin-1 (Panx1) membrane channel glycoprotein. We propose that suppression of inflammation through manipulation of Panx1 activity can stimulate epithelial cell progenitor (EPCP) engraftment...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29056973/rationale-and-design-of-the-first-double-blind-placebo-controlled-trial-with-allogeneic-adipose-tissue-derived-stromal-cell-therapy-in-patients-with-ischemic-heart-failure-a-phase-ii-danish-multicentre-study
#13
Jens Kastrup, Morten Schou, Ida Gustafsson, Olav W Nielsen, Rasmus Møgelvang, Klaus F Kofoed, Charlotte Kragelund, Jens Dahlgaard Hove, Andreas Fabricius-Bjerre, Merete Heitman, Mandana Haack-Sørensen, Lisbeth Drozd Lund, Ellen Mønsted Johansen, Abbas Ali Qayyum, Anders Bruun Mathiasen, Annette Ekblond
BACKGROUND: Ischemic heart failure (IHF) has a poor prognosis in spite of optimal therapy. We have established a new allogeneic Cardiology Stem Cell Centre adipose-derived stromal cell (CSCC_ASC) product from healthy donors. It is produced without animal products, in closed bioreactor systems and cryopreserved as an off-the-shelf product ready to use. STUDY DESIGN: A multicentre, double-blind, placebo-controlled phase II study with direct intramyocardial injections of allogeneic CSCC_ASC in patients with chronic IHF...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29053690/3d-microtissues-for-injectable-regenerative-therapy-and-high-throughput-drug-screening
#14
Yaqian Li, Xiaojun Yan, Wei Liu, Lyu Zhou, Zhifeng You, Yanan Du
To upgrade traditional 2D cell culture to 3D cell culture, we have integrated microfabrication with cryogelation technology to produce macroporous microscale cryogels (microcryogels), which can be loaded with a variety of cell types to form 3D microtissues. Herein, we present the protocol to fabricate versatile 3D microtissues and their applications in regenerative therapy and drug screening. Size and shape-controllable microcryogels can be fabricated on an array chip, which can be harvested off-chip as individual cell-loaded carriers for injectable regenerative therapy or be further assembled on-chip into 3D microtissue arrays for high-throughput drug screening...
October 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29033030/how-to-use-stem-cells-for-repair-in-stroke-patients
#15
REVIEW
O Detante, C Rome, J Papassin
Regenerative cell therapy is a promising therapeutic strategy in neurology, most notably to improve stroke recovery. Although tolerability and feasibility have apparently been validated, many questions remain as to what is the best type of cells to use, the best route and the post-stroke delay for administration. Two main strategies have currently emerged: intravenous injection of mesenchymal stem cells with systemic trophic support; and intracerebral grafting of neural stem cells with brain repair effects at the lesion site...
November 2017: Revue Neurologique
https://www.readbyqxmd.com/read/29019941/the-contribution-of-adipose-tissue-derived-mesenchymal-stem-cells-and-platelet-rich-plasma-to-the-treatment-of-chronic-equine-laminitis-a-proof-of-concept
#16
Mario Angelone, Virna Conti, Cristiano Biacca, Beatrice Battaglia, Laura Pecorari, Francesco Piana, Giacomo Gnudi, Fabio Leonardi, Roberto Ramoni, Giuseppina Basini, Silvia Dotti, Sabrina Renzi, Maura Ferrari, Stefano Grolli
Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes of the complex lamellar structures that maintain the appendicular skeleton within the hoof. Laminitis is a multifactorial disease that involves perturbation of the vascular, hematological, and inflammatory homeostasis of the foot. Interestingly, the pathogenesis of the disease resembles what is observed in metabolic syndromes and sepsis-induced organ failure in humans and animals. We hypothesized that local administration of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) might contribute to establishing an anti-inflammatory and pro-angiogenic environment, and could stimulate the injured tissue in order to restore its functional integrity...
October 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29018808/tenogenically-induced-allogeneic-peripheral-blood-mesenchymal-stem-cells-in-allogeneic-platelet-rich-plasma-2-year-follow-up-after-tendon-or-ligament-treatment-in-horses
#17
Charlotte Beerts, Marc Suls, Sarah Y Broeckx, Bert Seys, Aurélie Vandenberghe, Jeroen Declercq, Luc Duchateau, Martin A Vidal, Jan H Spaas
Poor healing of tendon and ligament lesions often results in early retirement of sport horses. Therefore, regenerative therapies are being explored as potentially promising treatment for these injuries. In this study, an intralesional injection was performed with allogeneic tenogenically induced mesenchymal stem cells and platelet-rich plasma 5-6 days after diagnosis of suspensory ligament (SL) (n = 68) or superficial digital flexor tendon (SDFT) (n = 36) lesion. Clinical, lameness and ultrasonographic evaluation was performed at 6 and 12 weeks...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28983091/therapeutic-potential-of-human-minor-salivary-gland-epithelial-progenitor-cells-in-liver-regeneration
#18
Chen Zhang, Yan Li, Xiang-Yu Zhang, Lei Liu, Hai-Zhou Tong, Ting-Lu Han, Wan-di Li, Xiao-Lei Jin, Ning-Bei Yin, Tao Song, Hai-Dong Li, Juan Zhi, Zhen-Min Zhao, Lin Lu
Liver disease is a serious problem affecting millions of people with continually increasing prevalence. Stem cell therapy has become a promising treatment for liver dysfunction. We previously reported on human minor salivary gland mesenchymal stem cells (hMSGMSCs), which are highly self-renewable with multi-potent differentiation capability. In this study, keratinocyte-like cells with self-regeneration and hepatic differentiation potential were isolated and characterized, and named human minor salivary gland epithelial progenitor cells (hMSG-EpiPCs)...
October 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28975732/a-novel-calcium-accumulating-peptide-gelatin-in-situ-forming-hydrogel-for-enhanced-bone-regeneration
#19
Beom Soo Jo, Yunki Lee, Jin Sook Suh, Yoon Shin Park, Hyun Jung Lee, Jue-Yeon Lee, Jaejin Cho, Gene Lee, Chong Pyoung Chung, Ki Dong Park, Yoon Jeong Park
Bioactive agents, including proteins and peptides, can be loaded into hydrogels to improve bone regenerative capacity with their controlled release. However, the current loading method has focused on physical mixing, which has limited release control. Therefore, alternative conjugation of bioactive agents with hydrogels is highly recommended. Direct chemical conjugation of synthetic peptides containing a functional moiety with a hydrogel would be ideal. Here, we synthesized a bioactive calcium accumulating peptide (CAP) containing a collagen binding motif (CBM), which can induce osteogenic differentiation...
October 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28972000/enhanced-therapeutic-and-long-term-dynamic-vascularization-effects-of-human-pluripotent-stem-cell-derived-endothelial-cells-encapsulated-in-a-nanomatrix-gel
#20
Shin-Jeong Lee, Young-Doug Sohn, Adinarayana Andukuri, Sangsung Kim, Jaemin Byun, Ji Woong Han, In-Hyun Park, Ho-Wook Jun, Young-Sup Yoon
BACKGROUND: Human pluripotent stem cell (hPSC)-derived endothelial cells (ECs) have limited clinical utility because of undefined components in the differentiation system and poor cell survival in vivo. Here, we aimed to develop a fully defined and clinically compatible system to differentiate hPSCs into ECs. Furthermore, we aimed to enhance cell survival, vessel formation, and therapeutic potential by encapsulating hPSC-ECs with a peptide amphiphile (PA) nanomatrix gel. METHODS: We induced differentiation of hPSCs into the mesodermal lineage by culturing on collagen-coated plates with a glycogen synthase kinase 3β inhibitor...
November 14, 2017: Circulation
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