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https://www.readbyqxmd.com/read/29780158/evaluation-of-the-factors-affecting-concerns-and-expectations-of-patients-undergoing-total-knee-arthroplasty-in-china
#1
Yang Li, Hong Cai, Hua Tian, Ke Zhang
BACKGROUND The aim of this study was to assess the level of concern and expectations of patients and their families of total knee arthroplasty (TKA), peri-operative procedures, postoperative rehabilitation, and outcome in a Chinese population. MATERIAL AND METHODS The study recruited 133 patients with osteoarthritis, scheduled to undergo primary elective TKA. Before surgery, the surgeon completed the Hospital for Special Surgery (HSS) Knee Score questionnaire on pain, function, range of motion (ROM), and muscle strength...
May 21, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29777430/the-artificial-humic-substance-hs1500-does-not-inhibit-photosynthesis-of-the-green-alga-desmodesmus-armatus-in-vivo-but-interacts-with-the-photosynthetic-apparatus-of-isolated-spinach-thylakoids-in-vitro
#2
Matthias Gilbert, Hanno Bährs, Christian E W Steinberg, Christian Wilhelm
Humic substances (HSs) can influence the growth and composition of freshwater phytoplankton assemblage. Since HSs contain many phenolic and quinonic moieties and cause growth reductions in eco-physiological field experiments, HSs are considered photosystem II herbicides. To test this specific mode of action in vivo and in vitro, respectively, we used intact cells of the green alga Desmodesmus armatus, as well as thylakoids isolated from spinach (Spinacia oleracea) as a model system for the green algal chloroplast...
May 18, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/29775810/a-validated-uplc-ms-ms-method-for-the-quantitation-of-an-unstable-peptide-monocyte-locomotion-inhibitory-factor-mlif-in-human-plasma-and-its-application-to-a-pharmacokinetic-study
#3
Xuemei Liu, Pei Hu, Yongsheng Wang, Xizhu Wang, Jinghua Huang, Jin Li, Cheng Li, Hongyun Wang, Ji Jiang
Monocyte locomotion inhibitory factor (MLIF, Met-Gln-Cys-Asn-Ser), a pentapeptide with anti-inflammatory activity, was developed for neural protection in acute ischemic stroke. Determination of MLIF in human plasma samples is of great importance for pharmacokinetic evaluation in clinical studies. A reliable and sensitive method based on ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was established for the measurement of MLIF in human plasma. Instability of peptide in matrix was the primary challenge in method development, which was properly resolved by addition of acidification reagents like sulfuric acid...
April 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29770583/angle-stable-interlocking-intramedullary-nails-for-tibial-plateau-fractures
#4
Peng Jia, Feng-Cheng Lu, Kifayat Ullah, Man Zhang, Yan-Sheng Dong, Chao Xiong, Zhi-Hui Zhao, Jin-Feng Wang, Ju-Wen Chen, Yong-Qing Wang
OBJECTIVE: Angle stable interlocking intramedullary nail (ASIN), a novel technique, has rarely been used for treatment of tibial plateau fractures (TPF). This retrospective study was designed to introduce this novel technique, ASIN, as well as to describe the initial experience and verify the effectiveness when ASIN was used for the management for TPF. METHODS: A cohort of 19 cases with closed TPF aged from 18-70 years with at least 23 months follow-up from November 2008 to September 2013 was analyzed retrospectively...
May 16, 2018: Orthopaedic Surgery
https://www.readbyqxmd.com/read/29769389/extracts-for-analysis-of-dna-replication-in-a-nucleus-free-system
#5
Justin Sparks, Johannes C Walter
Frog egg extracts represent a powerful approach with which to dissect molecular mechanisms of vertebrate DNA replication and repair. In the classical approach, sperm chromatin is added to a crude egg lysate to form replication-competent nuclei. We subsequently described a procedure that bypasses the requirement for nuclear assembly in DNA replication. In this method, DNA is first added to a high-speed supernatant (HSS) of egg lysate, which mimics the G1 phase of the cell cycle in that it supports replication licensing...
May 16, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29768657/simultaneous-quantification-of-fentanyl-sufentanil-cefazolin-doxapram-and-keto-doxapram-in-plasma-using-liquid-chromatography-tandem-mass-spectrometry
#6
Robert B Flint, Soma Bahmany, Bart C H van der Nagel, Birgit C P Koch
A simple and specific UPLC-MS/MS method was developed and validated for simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and its active metabolite keto-doxapram. The internal standard was fentanyl-d5 for all analytes. Chromatographic separation was achieved with a reversed phase Acquity UPLC HSS T3 column with a run-time of only 5.0 minutes per injected sample. Gradient elution was performed with a mobile phase consisting of ammonium acetate, formic acid in Milli-Q ultrapure water or in methanol with a total flow rate of 0...
May 16, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29762284/hss-dysphagia-and-dysphonia-inventory-hss-ddi-following-anterior-cervical-fusion-patient-derived-validated-condition-specific-patient-reported-outcome-measure-outperforms-existing-indices
#7
Alexander P Hughes, Stephan N Salzmann, Okezie K Aguwa, Courtney Ortiz Miller, Roland Duculan, Jennifer Shue, Frank P Cammisa, Andrew A Sama, Federico P Girardi, Ashutosh Kacker, Carol A Mancuso
BACKGROUND: Dysphagia and dysphonia are common complications after anterior cervical spine surgery; however, reported prevalences vary greatly due to a lack of reliable clinical standards for measuring postoperative swallowing and speech dysfunction. The Hospital for Special Surgery Dysphagia and Dysphonia Inventory (HSS-DDI) was developed as a patient-derived, patient-reported instrument to measure dysphagia and dysphonia more accurately after anterior cervical spine surgery than existing indices...
May 16, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29758069/strengthening-primary-eye-care-in-south-africa-an-assessment-of-services-and-prospective-evaluation-of-a-health-systems-support-package
#8
Rivka R Lilian, Jean Railton, Erik Schaftenaar, Moyahabo Mabitsi, Cornelis J Grobbelaar, N Sellina Khosa, Babra H Maluleke, Helen E Struthers, James A McIntyre, Remco P H Peters
Visual impairment is a significant public health concern, particularly in low- and middle-income countries where eye care is predominantly provided at the primary healthcare (PHC) level, known as primary eye care. This study aimed to perform an evaluation of primary eye care services in three districts of South Africa and to assess whether an ophthalmic health system strengthening (HSS) package could improve these services. Baseline surveys were conducted in Cape Winelands District, Johannesburg Health District and Mopani District at 14, 25 and 36 PHC facilities, respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29751428/molecular-composition-of-raw-peat-and-humic-substances-from-permafrost-peat-soils-of-european-northeast-russia-as-climate-change-markers
#9
Roman Vasilevich, Evgeny Lodygin, Vasily Beznosikov, Evgeny Abakumov
Humic substances (HSs) from the mire peat soils of the forest-tundra zone of the European northeast part of Russia have been characterized in terms of molecular composition. This was accomplished using solid-state 13 C nuclear magnetic resonance (13 C NMR) techniques and electron spin resonance (ESR) spectroscopy. The composition depended on the intensity of cryogenic processes in the active layer, the quality of the humification precursors (the degree of peat material transformation), and the biochemical selection of aromatic fragments during humification...
February 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29747473/supercritical-fluid-chromatography-with-photodiode-array-detection-in-the-determination-of-fat-soluble-vitamins-in-hemp-seed-oil-and-waste-fish-oil
#10
Katarzyna Tyśkiewicz, Roman Gieysztor, Izabela Maziarczyk, Paweł Hodurek, Edward Rój, Krystyna Skalicka-Woźniak
In the presented study for the first time a new, optimized, fast SFC (supercritical fluid chromatography) method was applied to separate in one run fat-soluble vitamins from waste fish oil, including cis -and tran s-retinyl palmitate, cis - and trans -retinyl acetate, retinol, α-tocopherol, β-tocopherol, γ‑tocopherol, δ-tocopherol, ergocalciferol (D₂), cholecalciferol (D₃), cis - and trans -phylloquinone (K₁) and menaquinone-4 (K₂-MK4). Vitamins were baseline separated on an Acquity UPC² (ultra performance convergence chromatography) HSS C18 SB (highly strength chemically modified silica) column within 13 min...
May 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29735900/synthesis-biological-evaluation-and-docking-studies-of-novel-bisquaternary-aldoxime-reactivators-on-acetylcholinesterase-and-butyrylcholinesterase-inhibited-by-paraoxon
#11
Kamil Kuca, Daniel Jun, Lucie Junova, Kamil Musilek, Martina Hrabinova, Jorge Alberto Valle da Silva, Teodorico Castro Ramalho, Marian Valko, Qinghua Wu, Eugenie Nepovimova, Tanos Celmar Costa França
Nerve agents and oxon forms of organophosphorus pesticides act as strong irreversible inhibitors of two cholinesterases in the human body: acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BChE; EC 3.1.1.8), and are therefore highly toxic compounds. For the recovery of inhibited AChE, antidotes from the group of pyridinium or bispyridinium aldoxime reactivators (pralidoxime, obidoxime, HI-6) are used in combination with anticholinergics and anticonvulsives. Therapeutic efficacy of reactivators (called “oximes”) depends on their chemical structure and also the type of organophosphorus inhibitor...
May 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29730081/eosinophilic-oesophagitis-endotype-classification-by-molecular-clinical-and-histopathological-analyses-a-cross-sectional-study
#12
Tetsuo Shoda, Ting Wen, Seema S Aceves, J Pablo Abonia, Dan Atkins, Peter A Bonis, Julie M Caldwell, Kelley E Capocelli, Christina L Carpenter, Margaret H Collins, Evan S Dellon, Michael D Eby, Nirmala Gonsalves, Sandeep K Gupta, Gary W Falk, Ikuo Hirano, Paul Menard-Katcher, Jonathan T Kuhl, Jeffrey P Krischer, John Leung, Vincent A Mukkada, Jonathan M Spergel, Michael P Trimarchi, Guang-Yu Yang, Nives Zimmermann, Glenn T Furuta, Marc E Rothenberg
BACKGROUND: Eosinophilic oesophagitis is understood in terms of quantifiable histological, endoscopic, and molecular features. Data are scant for inter-relations of these features and their potential to identify distinct disease endotypes. We aimed to identify clinical-pathological correlations between endoscopic and histological disease variables by transcription profiling of the oesophagus of patients with eosinophilic oesophagitis of varying severity and disease activity states. METHODS: We did a cross-sectional study across ten hospital sites in the USA associated with the Consortium of Eosinophilic Gastrointestinal Disease Researchers...
May 2, 2018: Lancet. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29716204/demixing-surface-nematization-and-competing-adsorption-in-binary-mixtures-of-hard-rods-and-hard-spheres-under-confinement
#13
Liang Wu, Alexandr Malijevský, Carlos Avendaño, Erich A Müller, George Jackson
A molecular simulation study of binary mixtures of hard spherocylinders (HSCs) and hard spheres (HSs) confined between two structureless hard walls is presented. The principal aim of the work is to understand the effect of the presence of hard spheres on the entropically driven surface nematization of hard rod-like particles at surfaces. The mixtures are studied using a constant normal-pressure Monte Carlo algorithm. The surface adsorption at different compositions is examined in detail. At moderate hard-sphere concentrations, preferential adsorption of the spheres at the wall is found...
April 28, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29701757/rapid-and-sensitive-quantification-of-ursolic-acid-and-oleanolic-acid-in-human-plasma-using-ultra-performance-liquid-chromatography-mass-spectrometry
#14
Larissa Stebounova, Scott M Ebert, Logan T Murry, Christopher M Adams, Daryl J Murry
Ultra-performance liquid chromatography (UPLC) interfaced with atmospheric pressure chemical ionization mass-spectrometry was used to separate and quantify ursolic acid (UA) and oleanolic acid (OA) in human plasma. UA and OA were extracted from 0.5 mL human plasma using supported liquid extraction and separated utilizing an Acquity UPLC HSS column. The method has been validated for both UA and OA quantitation with a limit of detection of 0.5 ng/mL. The UPLC separations are carried out with isocratic elution with methanol and 5 mM ammonium acetate in water (85:15) as a mobile phase at a flow rate of 0...
April 26, 2018: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29701041/-acupuncture-combined-with-femoral-nerve-block-for-postoperative-analgesia-after-total-knee-arthroplasty-and-functional-rehabilitation-a-randomized-controlled-trial
#15
Yonghui Zhang, Lin Zhang, Min Lu
OBJECTIVE: To observe the therapeutic effects on the postoperative analgesia after total knee arthroplasty (TKA) and functional rehabilitation with acupuncture at the distal points along the affected meridians and femoral nerve block. METHODS: A total of 60 patients with knee prosthesis were collected and randomized into an observation group and a control group, 30 cases in each one. All of the patients received the femoral nerve block before operation. The analgesia pump was used for analgesia after operation and TKA postoperative rehabilitation was adopted...
March 12, 2018: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29690729/-clinical-study-of-a-new-wearable-device-for-rehabilitation-after-total-knee-arthroplasty
#16
K Kang, Q Geng, H T Xu, X Z Zheng, J T Dong, T Li, Z G Zhao, S J Gao
Objective: To evaluate the effect of promoting knee joint rehabilitation after total knee arthroplasty (TKA) with a rehabilitation training instrument NEO-GAIT. Methods: Sixty patients who received TKA from January 2017 to July 2017 in the Third Hospital of Hebei Medical University were randomly assigned to receive rehabilitation training with continuous passive motion (CPM) or NEO-GAIT with random number (30 cases in CPM group, included 8 males and 22 females; 30 cases in NEO-GAIT group, included 6 males and 24 females)...
April 17, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29688986/hypertonic-saline-solution-reduces-microcirculatory-dysfunction-and-inflammation-in-a-rat-model-of-brain-death
#17
Cristiano de Jesus Correia, Roberto Armstrong, Priscila Oliveira de Carvalho, Rafael Simas, Daniele Cristina Janolli Sanches, Ana Cristina Breithaupt-Faloppa, Paulina Sannomiya, Luiz Felipe Pinho Moreira
BACKGROUND: Brain death (BD) induces hemodynamic instability with microcirculatory hypoperfusion, leading to increased organ inflammation and dysfunction. This study investigated the effects of 7.5% hypertonic saline solution (HSS) on mesenteric microcirculatory dysfunction and inflammation in a rat model of BD. METHODS: Male Wistar rats were anesthetized and mechanically ventilated. BD was induced by rapidly inflating an intracranial balloon catheter. The rats were randomly divided into: (1)SH, sham-operated rats subjected to trepanation; (2)NS, rats treated with NaCl 0...
April 23, 2018: Shock
https://www.readbyqxmd.com/read/29676127/-study-on-quality-control-of-holotrichia-diomphalia-larvae-by-tlc-and-hplc
#18
Yan-Tao Sun, Jing Sun, Lu-Hong Wang, Lei Zhao
The TLC method was established for identification of Holotricha diomphalia larvae and the HPLC method was used to determine the content of inosine and guanosine in H. diomphalia larvae. The HPLC analysis was performed on a Waters HSS T3(4.6 mm×250 mm, 5 μm) column of with mobile phase consisting of acetonitrile (A) and 0.08% trifluoroacetic acid (B) in gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL·min⁻¹. The column temperature was 30 °C. As a result, TLC identification method had a good reproducibility and highly specificity...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29669568/social-preferences-for-health-states-associated-with-acute-myeloid-leukemia-for-patients-undergoing-treatment-in-the-united-kingdom
#19
Nacho Castejón, Joseph C Cappelleri, Jesús Cuervo, Kathryn Lang, Priyanka Mehta, Ruth Mokgokong, Carla Mamolo
BACKGROUND: Health state (HS) utility values for patients with acute myeloid leukemia (AML), a hematological malignancy, are not available in the United Kingdom (UK). This study aims to develop clinically sound HSs for previously untreated patients with AML and to assign utility values based on preferences of the general UK population. METHODS: This study was conducted in the UK and comprised 2 stages. During the first stage, AML HSs were drafted based on evidence from a literature review of AML clinical and health-related quality-of-life studies (published January 2000-June 2016) and patient-reported outcome measures previously used in this population...
April 18, 2018: Health and Quality of Life Outcomes
https://www.readbyqxmd.com/read/29665717/selective-peripheral-nerve-resection-for-treatment-of-persistent-pain-around-the-knee-joint-after-total-knee-arthroplasty
#20
Guangjun Zhong, Zhu Liang, Jiang Kan, Aikeremujiang Muheremu
Objective This study was performed to determine the efficacy of selective peripheral nerve resection for treatment of persistent neuropathic pain after total knee arthroplasty (TKA). Methods Patients who underwent TKA in our department from January 2013 to July 2016 and experienced persistent pain around the knee joint after TKA were retrospectively included in the current study. Sixty patients were divided into experimental and control groups according the treatment they received. The treatment effect was evaluated by the Hospital for Special Surgery (HSS) knee score and visual analog scale (VAS) pain score preoperatively and at 1, 2, 3, 6, and 12 months postoperatively...
January 1, 2018: Journal of International Medical Research
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