Naoaki Sakata, Gumpei Yoshimatsu, Ryo Kawakami, Kazuaki Nakano, Teppei Yamada, Akihiro Yamamura, Hiroshi Nagashima, Shohta Kodama
Organoid is a tissue-engineered organ-like structure that resemble as an organ. Porcine islet-derived organoid might be used as an alternative donor of porcine islet xenotransplantation, a promising therapy for severe diabetes. In this study, we elucidated the characteristics of porcine islet organoids derived from porcine islets as a cell source for transplantation. Isolated porcine islets were 3D-cultured using growth factor-reduced matrigel in organoid culture medium consist of advanced DMEM/F12 with Wnt-3A, R-spondin, EGF, Noggin, IGF-1, bFGF, nicotinamide, B27, and some small molecules...
March 16, 2024: Scientific Reports