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https://www.readbyqxmd.com/read/28527412/early-stage-detection-of-ve-cadherin-during-endothelial-differentiation-of-human-mesenchymal-stem-cells-using-spr-biosensor
#1
Farzaneh Fathi, Aysa Rezabakhsh, Reza Rahbarghazi, Mohammad-Reza Rashidi
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. Up to present, SPR application in stem cell biology and biomedical sciences was underused. Herein, a very simple and sensitive method was developed to evaluate human mesenchymal stem cells trans-differentiation to endothelial lineage of over a period of 14 days based on VE-cadherin biomarker...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525266/gliotoxin-biosynthesis-structure-mechanism-and-metal-promiscuity-of-carboxypeptidase-glij
#2
Antoine Marion, Michael Groll, Daniel H Scharf, Kirstin Scherlach, Manuel Glaser, Holger Sievers, Michael Schuster, Christian Hertweck, Axel A Brakhage, Iris Antes, Eva M Huber
The formation of glutathione (GSH) conjugates, best known from the detoxification of xenobiotics, is a widespread strategy to incorporate sulfur into biomolecules. The biosynthesis of gliotoxin, a virulence factor of the human pathogenic fungus Aspergillus fumigatus, involves attachment of two GSH molecules and their sequential decomposition to yield two reactive thiol groups. The degradation of the GSH moieties requires the activity of the Cys-Gly carboxypeptidase GliJ, for which we describe the X-ray structure here...
May 19, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28512127/a-structure-derived-snap-trap-mechanism-of-a-multispecific-serpin-from-the-dysbiotic-human-oral-microbiome
#3
Theodoros Goulas, Miroslaw Ksiazek, Irene Garcia-Ferrer, Alicja M Sochaj-Gregorczyk, Irena Waligorska, Marcin Wasylewski, Jan Potempa, F Xavier Gomis-Rüth
Enduring host-microbiome relationships are based on adaptive strategies within a particular ecological niche. Tannerella forsythia is a dysbiotic member of the human oral microbiome that inhabits periodontal pockets and contributes to chronic periodontitis. To counteract endopeptidases from the host or microbial competitors, T. forsythia possesses a serpin-type proteinase inhibitor called miropin. While serpins from animals, plants, and viruses have been widely studied, those from prokaryotes have received only limited attention...
May 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28511507/evaluation-of-c-reactive-protein-and-fibrinogen-in-patients-with-chronic-and-aggressive-periodontitis-a-clinico-biochemical-study
#4
Swaroop Chandy, Kiran Joseph, Anila Sankaranarayanan, Annie Issac, George Babu, Bobby Wilson, Jumol Joseph
INTRODUCTION: Periodontal disease is characterised by chronic infection and inflammation in periodontal tissues leading to destruction of alveolar bone with subsequent tooth loss. Periodontal infections are the result of an interaction between tooth associated microbial biofilms and the host defences. Periodontal pathogens can affect local and systemic immune and inflammatory responses. AIM: The aim of the present study was to evaluate serum C-Reactive Protein (CRP), plasma fibrinogen and peripheral blood levels in healthy subjects, chronic and aggressive periodontitis patients...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28510428/statistical-analysis-and-prediction-of-covalent-ligand-targeted-cysteine-residues
#5
Weilin Zhang, Jianfeng Pei, Luhua Lai
Targeted covalent compounds or drugs have good potency as they can bind to a specific target for a long time with low doses. Most currently known covalent ligands were discovered by chance or by modifying existing non-covalent compounds to make them covalently attached to a nearby reactive residue. Computational methods for novel covalent ligand binding prediction are highly demanded. We performed statistical analysis on protein complexes with covalent ligands attached to cysteine residues. We found that covalent modified cysteine residues have unique features compared to those not attached to covalent ligands, including lower pKa, higher exposure and higher ligand binding affinity...
May 16, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28504171/tuneable-catechin-functionalisation-of-carbohydrate-polymers
#6
Susan Oliver, Aziidah Jofri, Donald S Thomas, Orazio Vittorio, Maria Kavallaris, Cyrille Boyer
In this contribution, we present a strategy to functionalise three natural carbohydrate polymers (dextran - a neutral polymer, sodium alginate - an anionic polymer and chitosan - a cationic polymer) with catechin with excellent degrees of functionality. In a first step, the carbohydrate polymers were oxidised by sodium periodate to yield aldehyde functionalised carbohydrate polymers. The presence of aldehyde groups was exploited to attach catechin by an acid catalysed nucleophilic reaction. The degree of catechin functionalisation could be easily tuned by varying the acid concentration in the reaction mixture, achieving catechin functionalisation levels of up to 48% for dextran aldehyde catechin, 35% for chitosan-aldehyde-catechin and 22% for sodium alginate aldehyde catechin...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28494698/extemporaneously-preparative-biodegradable-injectable-polymer-systems-exhibiting-temperature-responsive-irreversible-gelation
#7
Yasuyuki Yoshida, Kazuyuki Takata, Hiroki Takai, Keisuke Kawahara, Akinori Kuzuya, Yuichi Ohya
On clinical application of biodegradable injectable polymer (IP) systems, quick extemporaneous preparation of IP formulations and longer duration time gel state after injection into the body are the important targets to be developed. Previously, we had reported temperature-responsive covalent gelation systems via bio-orthogonal thiol-ene reaction by "mixing strategy" of amphiphilic biodegradable tri-block copolymer (tri-PCG) attaching acryloyl groups on both termini (tri-PCG-Acryl) with reactive polythiol. In other previous works, we found "freeze-dry with PEG/dispersion" method as quick extemporaneous preparation method of biodegradable IP formulations...
May 12, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28492359/periodontitis-and-endothelial-dysfunction-periodontal-clinical-parameters-and-the-levels-of-salivary-markers-il-1%C3%AE-tnf-%C3%AE-mmp2-timp2-complex-and-nitric-oxide
#8
Marcela Faria Moura, Túlio Pinho Navarro, Tarcília Aparecida Silva, Luís Otávio Miranda Cota, Alcione Maria Soares Dutra Oliveira, Fernando Oliveira Costa
BACKGROUND: Periodontitis has been associated with a greater risk for arteriosclerotic cardiovascular disease (ACD). Endothelial dysfunction (ED) is a parameter of early ACD, and its association with periodontitis has rarely been investigated to date. The objective of this study was to evaluate the association between periodontitis and ED by means of periodontal clinical parameters and salivary markers IL-1β, TNF-α, nitric oxide-(NO) and MMP2/TIMP2 complex. METHODS: Forty-seven individuals were divided into two groups: 24 individuals with chronic periodontitis and 23 individuals without chronic periodontitis...
May 11, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28485853/1-2-4-triazine-modified-2-deoxyuridine-triphosphate-for-efficient-bioorthogonal-fluorescent-labeling-of-dna
#9
Krisana Peewasan, Hans-Achim Wagenknecht
In order to establish the Diels-Alder reaction with inverse electron demand for postsynthetic DNA modification a 1,2,4-triazine-modified 2'-deoxyuridine triphosphate was synthesized. The bioorthogonally reactive 1,2,4-triazine group was attached at the 5-position of 2'-deoxyuridine by a flexible alkyl linker to facilitate its acceptance by DNA polymerases. The screening of four DNA polymerases showed successful primer extensions using a mixture of dATP, dGTP, dCTP and the modified 2'-deoxyuridine triphosphate with the KOD XL and Vent polymerases...
May 9, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28480509/oxidized-low-density-lipoprotein-stimulates-epithelial-sodium-channels-in-endothelial-cells-of-mouse-thoracic-aorta
#10
Chen Liang, Qiu-Shi Wang, Xu Yang, Na Niu, Qing-Qing Hu, Bao-Long Zhang, Ming-Ming Wu, Chang-Jiang Yu, Xiao Chen, Bin-Lin Song, Zhi-Ren Zhang, He-Ping Ma
BACKGROUND AND PURPOSE: Epithelial sodium channel (ENaC) is expressed in endothelial cells and acts as a negative modulator of vasodilation. Oxidized low-density lipoprotein (ox-LDL) is a key pathological factor in endothelial dysfunction. The present study is to examine the role of ENaC in ox-LDL-induced endothelial dysfunction and its associated signal transduction pathway. EXPERIMENTAL APPROACH: Patch-clamp techniques combined with pharmacological approaches were used to examine ENaC activity in the endothelial cells of split-open mouse thoracic aorta...
May 8, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28476317/evolution-structure-and-membrane-association-of-ndufaf6-an-assembly-factor-for-nadh-ubiquinone-oxidoreductase-complex-i
#11
Bernard D Lemire
The NADH:ubiquinone oxidoreductase (complex I) is the largest member of the mitochondrial respiratory chain. Its FMN cofactor accepts two electrons from NADH and transfers them to ubiquinone via a chain of iron-sulphur centers. A central core of 14 highly conserved subunits can couple electron transfer to proton translocation. The mammalian enzyme has an additional ~30 accessory subunits. Complex I has important bioenergetic and metabolic functions and is a known source of reactive oxygen species; these functions link it to a number of hereditary and degenerative diseases...
May 2, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28474233/association-analysis-of-rs1049255-and-rs4673-transitions-in-p22phox-gene-with-coronary-artery-disease-a-case-control-study-and-a-computational-analysis
#12
M Mazaheri, M Karimian, M Behjati, F Raygan, A Hosseinzadeh Colagar
BACKGROUND: The p22phox gene encodes the main subunit of NADH/NADPH-oxidase. This enzyme is expressed in smooth muscle cells of arteries, and it produces the reactive oxygen species. On the other hand, oxidative stress plays a main role in the pathogenesis of coronary artery disease (CAD). AIM: The aim of this study is to evaluate the association between rs4673 and rs1049255 polymorphisms of p22phox gene with CAD in an Iranian population which was followed with a computational analysis approach...
May 4, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/28467697/olefin-cross-metathesis-in-polymer-and-polysaccharide-chemistry-a-review
#13
Yifan Dong, John B Matson, Kevin J Edgar
Olefin cross-metathesis, a ruthenium-catalyzed carbon-carbon double bond transformation that features high selectivity, reactivity, and tolerance of various functional groups, has been extensively applied in organic synthesis and polymer chemistry. Herein, we review strategies for performing selective cross-metathesis and its applications in polymer and polysaccharide chemistry, including constructing complex polymer architectures, attaching pendant groups to polymer backbones and surfaces, and modifying polysaccharide derivatives...
May 18, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28463781/visible-and-uva-light-as-a-potential-means-of-preventing-escherichia-coli-biofilm-formation-in-urine-and-on-materials-used-in-urethral-catheters
#14
T L Vollmerhausen, A Conneely, C Bennett, V E Wagner, J C Victor, C P O'Byrne
Catheter-associated urinary tract infections are the most common hospital-acquired infection, for which Escherichia coli is the leading cause. This study investigated the efficacy of 385nm and 420nm light for inactivation of E. coli attached to the silicone matrix of a urinary catheter. Using urine mucin media, inactivation of planktonic bacteria and biofilm formation was monitored using silicone coupons. Continuous irradiance with both 385nm and 420nm wavelengths with starting cell density population 10(3)CFU ml(-1) reduced planktonic suspensions of E...
April 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28459216/abrus-agglutinin-targets-cancer-stem-like-cells-by-eliminating-self-renewal-capacity-accompanied-with-apoptosis-in-oral-squamous-cell-carcinoma
#15
Niharika Sinha, Prashanta Kumar Panda, Prajna Paramita Naik, Tapas K Maiti, Sujit K Bhutia
The accumulating evidences show that Abrus agglutinin, a plant lectin, displays a broad range of anticancer activity including cancer-specific induction of apoptosis; however, the underlying molecular mechanism of Abrus agglutinin-induced oral cancer stem cell elimination remains elusive. Our data documented that Abrus agglutinin effectively downregulated the CD44(+) expression with the increased CD44(-) population in different oral cancer cells. After 24-h Abrus agglutinin treatment, FaDu cells were quantified for orosphere formation in ultra-low attachment plates and data showed that Abrus agglutinin inhibited the number and size of orosphere in a dose-dependent manner in FaDu cells...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28458241/a-novel-pyridyl-triphenylamine-bodipy-aldoxime-naked-eye-visible-and-fluorometric-chemodosimeter-for-hypochlorite
#16
Xiu-Xiu Xu, Ying Qian
An aldoxime containing fluorescent probe based on vinylpydine-appended triphenylamine-BODIPY has been designed and used for hypochlorite detection. OX-PPA-BODIPY was developed by introducing an aldoxime group into the 2-position of BODIPY, which can be used for the detection of hypochlorite with a sharp color change from pink to green. The attachment of 4-vinylpyridine moiety to triphenylamine-BODIPY constructs a fluorogen with desirable conjugated system. The probe, which displays extremely weak fluorescence owing to the CN isomerization mechanism at 2-position of BODIPY, responds to HClO/ClO(-) through a dramatic enhancement of its fluorescence intensity...
April 20, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28458202/toxicity-of-%C3%AE-fe2o3-nanoparticles-to-artemia-salina-cysts-and-three-stages-of-larvae
#17
Chunjie Wang, Huali Jia, Lili Zhu, Hui Zhang, Yunsheng Wang
Artemia salina cysts (capsulated and decapsulated) and larvae (instar I, II and III) were exposed to α-Fe2O3 nanoparticles (α-Fe2O3-NPs) to evaluate the effects on marine ecosystems. Hatchability, mortality and a number of ethological, morphological and biochemical parameters were selected as end-points to define the toxic responses. Results indicate that the hatchability of capsulated and decapsulated cysts was significantly decreased (p<0.01) following exposure to 600mg/L at 12, 18, 24 and 36h. Both increases of mortality and decreases of swimming speed were shown concentration-dependent manners...
April 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28451135/resolving-the-%C3%AE-glycosidic-linkage-of-arginine-rhamnosylated-translation-elongation-factor-p-triggers-generation-of-the-first-arg-rha-specific-antibody
#18
Xiang Li, Ralph Krafczyk, Jakub Macošek, Yu-Lei Li, Yan Zou, Bernd Simon, Xing Pan, Qiu-Ye Wu, Fang Yan, Shan Li, Janosch Hennig, Kirsten Jung, Jürgen Lassak, Hong-Gang Hu
A previously discovered posttranslational modification strategy - arginine rhamnosylation - is essential for elongation factor P (EF-P) dependent rescue of polyproline stalled ribosomes in clinically relevant species such as Pseudomonas aeruginosa and Neisseria meningitidis. However, almost nothing is known about this new type of N-linked glycosylation. In the present study we used NMR spectroscopy to show for the first time that the α anomer of rhamnose is attached to Arg32 of EF-P, demonstrating that the corresponding glycosyltransferase EarP inverts the sugar of its cognate substrate dTDP-β-l-rhamnose...
December 1, 2016: Chemical Science
https://www.readbyqxmd.com/read/28445622/thiocarbohydrates-on-gold-nanoparticles-strong-influences-of-stereocenters-on-binding-affinitiy-and-interparticle-forces
#19
Torsten Linker, Prashant Pavashe, Werner Fudickar
Carbohydrates carrying thiol groups at the C-2 position have been attached to gold nanoparticles (AuNP) with stereocenters in close proximity to the surface for the first time. Their configurations can be clearly distinguished by the tendency of particle aggregation. AuNP surface plasmon resonance (SPR), x-ray photoelectron spectroscopy (XPS), and IR spectroscopy indicate that the thiocarbohydrates replace citrate molecules at different rates causing aggregation and eventually precipitation. A quantitative formulation of this aggregation process shows that reactivities can vary by several magnitudes...
April 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28444900/lactosylceramide-induced-phosphorylation-signaling-to-group-iva-phospholipase-a2-via-reactive-oxygen-species-in-tumor-necrosis-factor-%C3%AE-treated-cells
#20
Hiroyuki Nakamura, Yuta Moriyama, Kazuaki Watanabe, Satoshi Tomizawa, Risa Yamazaki, Hiromasa Takahashi, Toshihiko Murayama
The activity of α-type cytosolic phospholipase A2 (cPLA2 α, group IVA PLA2 ), which releases arachidonic acid (AA), is mainly regulated by the Ca(2+) -induced intracellular translocation/attachment of the enzyme to substrate membranes and its phosphorylation. We previously reported that tumor necrosis factor-α (TNFα) stimulated the formation of lactosylceramide (LacCer) in L929 fibroblast cells, and this lipid directly bound with and activated cPLA2 α [Nakamura et al., J. Biol. Chem. 2013;288:23264-23272]...
April 26, 2017: Journal of Cellular Biochemistry
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