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Reactive Attachment

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https://www.readbyqxmd.com/read/29792811/tandem-mass-spectrometry-of-peptide-ions-by-microwave-excited-hydrogen-and-water-plasmas
#1
Yuji Shimabukuro, Hidenori Takahashi, Shinichi Iwamoto, Koichi Tanaka, Motoi Wada
A thermal cracking cell that served as the atomic hydrogen source for hydrogen attachment/abstraction dissociation (HAD) analysis has an intrinsic problem to produce a beam of atoms reactive against heated tungsten capillary. A plasma excited by 2.45 GHz microwave power can deliver reactive species to a quadrupole ion trap confining analyte ions without excessive heating of the radical source components. The radical (H•) production performance of the developed source was evaluated by optical emission spectroscopy and H• attachment reaction to fullerene ions...
May 24, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29783198/toxicity-assessment-of-metal-oxide-nano-pollutants-on-tomato-solanum-lycopersicon-a-study-on-growth-dynamics-and-plant-cell-death
#2
Bilal Ahmed, Mohammad Saghir Khan, Javed Musarrat
The present study for the first time demonstrated the interactions of metal oxide (MO) nano-pollutants (CuO and Al2 O3 -NPs) with tissues and cellular DNA of tomato plants grown in soil sand: silt: clay (667:190:143) and Hoagland-hydroponic system and assessed the hazardous effects of NPs on cell physiology and biochemistry. Results of SEM equipped with EDX revealed attachment of variably shaped CuO-NPs (18 nm) and Al2 O3 -NPs (21 nm) on roots, and internalization followed by translocation in plants by ICP-MS and TEM...
May 18, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29780497/the-development-of-a-novel-and-logic-based-fluorescence-probe-for-the-detection-of-peroxynitrite-and-gsh
#3
Adam C Sedgwick, Hai-Hao Han, Jordan E Gardiner, Steven D Bull, Xiao-Peng He, Tony D James
We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO- and GSH ( GSH-PF3 ). The GSH-PF3 probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, PF3 . GSH-PF3 produced only a small fluorescence response towards the addition of GSH or ONOO- separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29776088/how-nonuniform-contact-profiles-of-t-cell-receptors-modulate-thymic-selection-outcomes
#4
Hanrong Chen, Arup K Chakraborty, Mehran Kardar
T cell receptors (TCRs) bind foreign or self-peptides attached to major histocompatibility complex (MHC) molecules, and the strength of this interaction determines T cell activation. Optimizing the ability of T cells to recognize a diversity of foreign peptides yet be tolerant of self-peptides is crucial for the adaptive immune system to properly function. This is achieved by selection of T cells in the thymus, where immature T cells expressing unique, stochastically generated TCRs interact with a large number of self-peptide-MHC; if a TCR does not bind strongly enough to any self-peptide-MHC, or too strongly with at least one self-peptide-MHC, the T cell dies...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29774111/ceragenin-csa-13-as-free-molecules-and-attached-to-magnetic-nanoparticle-surfaces-induce-caspase-dependent-apoptosis-in-human-breast-cancer-cells-via-disruption-of-cell-oxidative-balance
#5
Ewelina Piktel, Izabela Prokop, Urszula Wnorowska, Grzegorz Król, Mateusz Cieśluk, Katarzyna Niemirowicz, Paul B Savage, Robert Bucki
Natural antimicrobial peptides and ceragenins, as non-peptide amphipathic mimics, have been proposed as anti-cancer agents. To date, it has been confirmed that cathelicidin LL-37 and ceragenin CSA-13, both in free form and immobilized on the surface of magnetic nanoparticles (MNP@LL-37, MNP@CSA-13) induce apoptosis in colon cancer cells. Nevertheless, the question remains whether ceragenins, as synthetic analogs of LL-37 peptide and mimicking a number of its properties, act as antineoplastic agents in breast cancer cells, where LL-37 peptide stimulates oncogenesis...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29773141/the-application-of-the-hyper-fluorescent-sensor-in-the-real-time-detection-of-h-2-o-2-in-babesia-bovis-merozoites-in-vitro
#6
Masahito Asada, Hassan Hakimi, Shin-Ichiro Kawazu
In recent years, genetically encoded fluorescent probes have allowed a dramatic advancement in time-lapse imaging, enabling this imaging modality to be used to investigate intracellular events in several apicomplexan parasite species. In this study, we constructed a plasmid vector to stably express a genetically encoded H2 O2 sensor probe called HyPer in Babesia bovis. The HyPer-transfected parasite population was successfully developed and subjected to a time-lapse imaging analysis under in vitro culture conditions...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29769764/association-of-pentraxin-and-high-sensitive-c-reactive-protein-as-inflammatory-biomarkers-in-patients-with-chronic-periodontitis-and-peripheral-arterial-disease
#7
Ramanarayana Boyapati, Srikanth Chinthalapani, Arpita Ramisetti, Shyam Sunder Salavadhi, Radhika Ramachandran
Context: Inflammation is a common feature of both peripheral artery disease (PAD) and periodontal disease. Aim: The aim of this study is to evaluate the relationship between PAD and periodontal disease by examining the levels of inflammatory cytokines, pentraxin-3 (PTX-3), and high-sensitive C-reactive protein from serum. Materials and Methods: A total of 50 patients were included in this cross-sectional study. Patients were divided into two groups: those with PAD (test group) and those with the non-PAD group (control group) based on ankle-brachial index values...
March 2018: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29765680/the-relationship-between-substrate-morphology-and-biological-performances-of-nano-silver-loaded-dopamine-coatings-on-titanium-surfaces
#8
Weibo Zhang, Shuang Wang, Shaohua Ge, Jialong Chen, Ping Ji
Biomedical device-associated infection (BAI) and lack of osseointegration are the main causes of implant failure. Therefore, it is imperative for implants not only to depress microbial activity and biofilm colonization but also to prompt osteoblast functions and osseointegration. As part of the coating development for implants, the interest of in vitro studies on the interaction between implant substrate morphology and the coating's biological performances is growing. In this study, by harnessing the adhesion and reactivity of bioinspired polydopamine, nano-silver was successfully anchored onto micro/nanoporous as well as smooth titanium surfaces to analyse the effect of substrate morphology on biological performances of the coatings...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29765073/oncotically-driven-control-over-glycocalyx-dimension-for-cell-surface-engineering-and-protein-binding-in-the-longitudinal-direction
#9
Erika M J Siren, Rafi Chapanian, Iren Constantinescu, Donald E Brooks, Jayachandran N Kizhakkedathu
Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing for reactivity control in the longitudinal direction ('z'-direction) on the glycocalyx. Studies in HUVECs demonstrated an oncotically driven collapse of the glycocalyx brush structure in the presence of crowders as the mechanism responsible for re-directing reactivity...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29749224/photoactivatable-rhodamine-spiroamides-and-diazoketones-decorated-with-universal-hydrophilizer-or-hydroxyl-groups
#10
Benoit Roubinet, Matthias Bischoff, Shamil Nizamov, Sergey Yan, Claudia Geisler, Stefan Stoldt, Gyuzel Y Mitronova, Vladimir N Belov, Mariano L Bossi, Stefan W Hell
Photoactivatable rhodamine spiroamides and spirocyclic diazoketones emerged recently as synthetic markers applicable in multicolor superresolution microscopy. However, their applicability in single molecule localization microscopy (SMLM) is often limited by aggregation, unspecific adhesion and low reactivity caused by insufficient solubility and precipitation from aqueous solutions. We report here two synthetic modifications increasing the polarity of compact polycyclic and hydrophobic labels decorated with a reactive group: attachment of 3-sulfo-L-alanyl - beta-alanine dipeptide (a "universal hydrophilizer") or allylic hydroxylation in photosensitive rhodamine diazoketones (and spiroamides)...
May 11, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29746101/systematic-approach-to-organizing-structural-alerts-for-reactive-metabolite-formation-from-potential-drugs
#11
Alf Claesson, Alexander Minidis
Reactive metabolites are widely accepted as playing a pivotal role in causing idiosyncratic adverse drug reactions (IDR). However, much is unknown about the biological mechanisms of IDR although the initiating event in most cases is an attachment of a reactive intermediate to macromolecules leading to immune mediated responses. Reactive metabolites are also involved in many mutagenesis/ carcinogenesis events by reacting with DNA. Drug designers thus have all reasons to make large efforts to avoid making test compounds having a liability to generate reactive metabolites...
May 10, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29742899/high-temperature-shape-memory-behavior-of-semi-crystalline-polyamide-thermosets
#12
Ming Li, Qingbao Guan, Theo J Dingemans
We have explored semi-crystalline poly(decamethylene terephthalamide) (PA 10T) based thermosets as single-component high-temperature (> 200 °C) shape memory polymers (SMPs). The PA 10T thermosets were prepared from reactive thermoplastic precursors. Reactive phenylethynyl (PE) functionalities were either attached at the chain termini or placed as side-groups along the polymer main-chain. The shape fixation and recovery performance of the thermoset films were investigated using a rheometer in torsion mode...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29742255/circulation-levels-of-acute-phase-proteins-pentraxin-3-and-serum-amyloid-a-in-atherosclerosis-have-correlations-with-periodontal-inflamed-surface-area
#13
Başak Temelli, Zuhal Yetkin Ay, Hasan Basri Savaş, Fatih Aksoy, Duygu Kumbul Doğuç, Ersin Uskun, Ercan Varol
Objectives One of the plausible mechanisms in the relationship between periodontitis and coronary artery disease (CAD) is the systemic inflammatory burden comprised of circulating cytokines/mediators related to periodontitis. This study aims to test the hypothesis that periodontal inflamed surface area (PISA) is correlated with higher circulating levels of acute phase reactants (APR) and pro-inflammatory cytokines/mediators and lower anti-inflammatory cytokines/mediators in CAD patients. Material and Methods Patients aged from 30 to 75 years who underwent coronary angiography with CAD suspicion were included...
2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29741159/fluorescent-single-digit-detonation-nanodiamond-for-biomedical-applications
#14
Nicholas Nunn, Marta d'Amora, Neeraj Prabhakar, Alexander M Panich, Natalya Froumin, Marco D Torelli, Igor Vlasov, Philipp Reineck, Brant Gibson, Jessica M Rosenholm, Silvia Giordani, Olga Shenderova
Detonation nanodiamonds (DNDs) have emerged as promising candidates for a variety of biomedical applications, thanks to different physicochemical and biological properties, such as small size and reactive surfaces. In this study, we propose carbon dot decorated single digit (4-5 nm diameter) primary particles of detonation nanodiamond as promising fluorescent probes. Due to their intrinsic fluorescence originating from tiny (1-2 atomic layer thickness) carbonaceous structures on their surfaces, they exhibit brightness suitable for in vitro imaging...
May 9, 2018: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/29736249/activation-of-disease-resistance-against-botryosphaeria-dothidea-by-downregulating-the-expression-of-mdsyp121-in-apple
#15
Xiaowen He, Yanhong Huo, Xiuxia Liu, Qianqian Zhou, Shouqian Feng, Xiang Shen, Baohua Li, Shujing Wu, Xuesen Chen
In plants, the vesicle fusion process plays a vital role in pathogen defence. However, the importance of the vesicle fusion process in apple ring rot has not been studied. Here, we isolated and characterised the apple syntaxin gene MdSYP121 . Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection; this increased tolerance was correlated with salicylic acid (SA) synthesis-related and signalling-related gene transcription. In contrast, overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes...
2018: Horticulture Research
https://www.readbyqxmd.com/read/29725541/cellulose-nanocrystal-zero-valent-iron-nanocomposites-for-groundwater-remediation
#16
Nathan Bossa, Alexis Wells Carpenter, Naresh Kumar, Charles-François de Lannoy, Mark Wiesner
Zero-valent iron nanoparticles (nano-ZVIs) have been widely studied for in situ remediation of groundwater and other environmental matrices. Nano-ZVI particle mobility and reactivity are still the main impediments in achieving efficient in situ groundwater remediation. Compared to the nano-ZVI "coating" strategy, nano-ZVI stabilization on supporting material allows direct contact with the contaminant, reduces the electron path from the nano-ZVI to the target contaminant and increases nano-ZVI reactivity...
June 1, 2017: Environmental Science. Nano
https://www.readbyqxmd.com/read/29725124/active-site-gate-dynamics-modulate-the-catalytic-activity-of-the-ubiquitination-enzyme-e2-25k
#17
Manoj K Rout, Brian L Lee, Aiyang Lin, Wei Xiao, Leo Spyracopoulos
The ubiquitin proteasome system (UPS) signals for degradation of proteins through attachment of K48-linked polyubiquitin chains, or alterations in protein-protein recognition through attachment of K63-linked chains. Target proteins are ubiquitinated in three sequential chemical steps by a three-component enzyme system. Ubiquitination, or E2 enzymes, catalyze the central step by facilitating reaction of a target protein lysine with the C-terminus of Ub that is attached to the active site cysteine of the E2 through a thioester bond...
May 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29724527/contribution-of-nitric-oxide-and-protein-s-nitrosylation-to-variation-in-fresh-meat-quality
#18
REVIEW
Rui Liu, Robyn Dorothy Warner, Guanghong Zhou, Wangang Zhang
As a primary source of reactive nitrogen species, nitric oxide (NO) is a signaling molecule playing multiple roles in physiological processes. NO exerts these pleiotropic effects mainly through the covalent attachment to the sulfhydryl group of protein cysteines to form S-nitrosothiol (protein S-nitrosylation). It has been two decades since NO was first investigated for its role in meat tenderization. Progress has been made, including studies by manipulating the NO levels in muscle cells, suggesting possible effects in the pre-slaughter and post-slaughter environment...
April 30, 2018: Meat Science
https://www.readbyqxmd.com/read/29723438/chronic-periodontitis-is-associated-to-erectile-dysfunction-a-case-control-study-in-european-population
#19
Amada Martín, Manuel Bravo, Miguel Arrabal, Antonio Magán-Fernández, Francisco Mesa
AIM: To determine the association between chronic periodontitis and erectile dysfunction adjusting for biochemical markers and other co-morbidities. METHODS: A case-control study was conducted on 158 male patients. 80 cases with erectile dysfunction according to the International Index of Erectile Function, and 78 controls. Sociodemographic data were gathered and a periodontal examination was performed. Testosterone, lipid profile, C-reactive protein and glycaemic parameters were assessed...
May 3, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29723214/micro-nano-hierarchical-structured-titanium-treated-by-nh4oh-h2o2-for-enhancing-cell-response
#20
Xin Yuan, Yi Kang, Jun Zuo, Youneng Xie, Li Ma, Xuelei Ren, Zeyu Bian, Qiuping Wei, Kechao Zhou, Xiyang Wang, Zhiming Yu
In this paper, two kinds of titanium surfaces with novel micro/nano hierarchical structures, namely Etched (E) surface and Sandblast and etched (SE) surface, were successfully fabricated by NH4OH and H2O2 mixture. And their cellular responses of MG63 were investigated compared with Sandblast and acid-etching (SLA) surface. Scanning electron microscope (SEM), Surface profiler, X-ray photoelectron spectroscopy (XPS), and Contact angle instrument were employed to assess the surface morphologies, roughness, chemistry and wettability respectively...
2018: PloS One
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