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Reactive Attachment

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https://www.readbyqxmd.com/read/28821094/decoration-of-open-pore-network-in-polyvinylidene-fluoride-mwcnts-with-chitosan-for-the-removal-of-reactive-orange-16-dye
#1
Maria Wasim, Sadia Sagar, Aneela Sabir, Muhammad Shafiq, Tahir Jamil
Inspired by the hydrophilic, biocompatible, adsorbent properties of chitosan. A simple, adaptable, green synthesis method was developed to prepare a thin film composite membranes using chitosan as a pore decorating material for the removal of anionic dye-Reactive orange 16 (RO-16). The hydrophilic chitosan was used to fill up the porous hydrophobic PVDF substrate, modified by MWCNTs. The dye rejection was carried out through the formation of a strong electrostatic interaction between the cationic group of chitosan surface and the anionic group of RO-16 dye...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28817148/effect-of-the-solvation-state-of-electron-in-dissociative-electron-attachment-reaction-in-aqueous-solutions
#2
Furong Wang, Pierre Archirel, Yusa Muroya, Shinichi Yamashita, Pascal Pernot, Chengying Yin, Abdel Karim El Omar, Uli Schmidhammer, Jean-Marie Teuler, Mehran Mostafavi
It is generally considered that the pre-solvated electron and the solvated electron reacting with a solute yield the same product. Silver cyanide complex, Ag(CN)2(-), is used as a simple probe to demonstrate unambiguously the existence of a different reduction mechanism for pre-hydrated electrons. Using systematic multichannel transient absorption measurements at different solute concentrations from millimolar to decimolar, global data analysis and theoretical calculations, we present the dissociative electron attachment on Ag(CN)2(-)...
August 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28811868/selaginella-bryopteris-aqueous-extract-improves-stability-and-function-of-cryopreserved-human-mesenchymal-stem-cells
#3
Abhishek Kumar Singh, Anubhuti Jha, Arindam Bit, Andrey P Kiassov, Albert A Rizvanov, Archit Ojha, Pooja Bhoi, Pradeep Kumar Patra, Awanish Kumar, Akalabya Bissoyi
The effective long-term cryopreservation of human mesenchymal stem cells (MSCs) is an essential prerequisite step and represents a critical approach for their sustained supply in basic research, regenerative medicine, and tissue engineering applications. Therefore, attempts have been made in the present investigation to formulate a freezing solution consisting of a combination of Selaginella bryopteris water-soluble extract with and without dimethyl sulfoxide (Me2SO) for the efficient long-term storage of human umbilical cord blood- (hUCB-) derived MSCs...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28803469/chiral-ceramic-nanoparticles-of-tungsten-oxide-and-peptide-catalysis
#4
Shuang Jiang, Mahshid Chekini, Zhi-Bei Qu, Yichun Wang, Aydan Yeltik, Yuangang Liu, Alex Kotlyar, Tianyong Zhang, Bin Li, Hilmi Volkan Demir, Nicholas A Kotov
The chirality of nanoparticles (NPs) and their assemblies has been investigated predominantly for noble metals and II-VI semiconductors. However, ceramic NPs represent the majority of nanoscale materials in Nature. The robustness and other innate properties of ceramics offer technological opportunities in catalysis, biomedical sciences, and optics. Here we report the preparation of chiral ceramic NPs, as represented by tungsten oxide hydrate, WO3-x·H2O, dispersed in ethanol. The chirality of the metal oxide core, with an average size of ca 1...
August 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28801268/positron-emission-tomography-pet-guided-glioblastoma-targeting-by-a-fullerene-based-nanoplatform-with-fast-renal-clearance
#5
Yayun Peng, Dongzhi Yang, Weifei Lu, Xiongwei Hu, Hao Hong, Ting Cai
Various carbonaceous nanomaterials, including fullerene, carbon nanotube, graphene, and carbon dots, have attracted increasing attention during past decades for their potential applications in biological imaging and therapy. In this study, we have developed a fullerene-based tumor-targeted positron emission tomography (PET) imaging probe. Water-soluble functionalized C60 conjugates were radio-labeled with (64)Cu and modified with cyclo (Arg-Gly-Asp) peptides (cRGD) for targeting of integrin αvβ3 in glioblastoma...
August 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28800217/engineering-antibody-reactivity-for-efficient-derivatization-to-generate-nav1-7-inhibitory-gptx-1-peptide-antibody-conjugates
#6
Kaustav Biswas, Thomas E Nixey, Justin K Murray, James R Falsey, Li Yin, Hantao Liu, Jacinthe Gingras, Brian E Hall, Brad Herberich, Jerry Ryan Holder, Hongyan Li, Joseph Ligutti, Min-Hwa Jasmine Lin, Dong Liu, Brian D Soriano, Marcus Soto, Linh Tran, Christopher M Tegley, Anrou Zou, Kannan Gunasekaran, Bryan D Moyer, Liz Doherty, Les P Miranda
The voltage-gated sodium channel NaV1.7 is a genetically-validated pain target under investigation for the development of analgesics. A therapeutic with a less frequent dosing regimen would be of value for treating chronic pain, however functional NaV1.7 targeting antibodies are not known. In this report we describe NaV1.7 inhibitory peptide-antibody conjugates as an alternate construct for potential prolonged channel blockade through chemical derivatization of engineered antibodies. We previously identified NaV1...
August 11, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28799332/attaching-the-nora-efflux-pump-inhibitor-inf55-to-methylene-blue-enhances-antimicrobial-photodynamic-inactivation-of-methicillin-resistant-staphylococcus-aureus-in-vitro-and-in-vivo
#7
Ardeshir Rineh, Naveen K Dolla, Anthony R Ball, Maria Magana, John B Bremner, Michael R Hamblin, George P Tegos, Michael J Kelso
Antimicrobial photodynamic inactivation (aPDI) uses photosensitizers (PSs) and harmless visible light to generate reactive oxygen species (ROS) and kill microbes. Multidrug efflux systems can moderate the phototoxic effects of PSs by expelling the compounds from cells. We hypothesized that increasing intracellular concentrations of PSs by inhibiting efflux with a covalently attached efflux pump inhibitor (EPI) would enhance bacterial cell phototoxicity and reduce exposure of neighbouring host cells to damaging ROS...
August 11, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28797194/contributions-of-early-adversity-to-pro-inflammatory-phenotype-in-infancy-the-buffer-provided-by-attachment-security
#8
Jeffrey R Measelle, Jennifer C Ablow
Adversity early in life is associated with systemic inflammation by adolescence and beyond. At present, few studies have investigated the associations between different forms of adversity and inflammation during infancy, making it difficult to specify the origins of disease vulnerability. This study examined the association between multiple forms of early adversity - socioeconomic status disadvantage, familial stress, maternal depression, and security of attachment - and individual differences in a composite measure of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and tumor necrosis factor-alpha) and the inflammatory protein C-reactive protein that were collected via saliva when (n = 49) children were 17 months old...
August 11, 2017: Attachment & Human Development
https://www.readbyqxmd.com/read/28795566/nucleotide-dependence-of-subunit-rearrangements-in-short-form-rubisco-activase-from-spinach
#9
Dayna S Peterson-Forbrook, Matthew T Hilton, Laura Tichacek, J Nathan Henderson, Hoang Quoc Bui, Rebekka M Wachter
Higher plant Rubisco activase (Rca) plays a critical role in regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In vitro, Rca is known to undergo dynamic assembly-disassembly processes, with several oligomer stoichiometries coexisting over a broad concentration range. Although the hexamer appears to be the active form, changes in quaternary structure could play a role in Rubisco regulation. Therefore, fluorescent labels were attached to the C-termini of spinach beta-Rca, and the rate of subunit mixing was monitored by measuring energy transfer as a function of nucleotide and divalent cation...
August 10, 2017: Biochemistry
https://www.readbyqxmd.com/read/28791819/decreased-sl-selectin-serum-levels-in-sleep-apnea-syndrome-patients-with-cardiovascular-diseases
#10
Monika Kosacka, Irena Porębska, Renata Jankowska
BACKGROUND: Obstructive sleep apnea syndrome (OSA) is a common disorder associated with an increased risk of cardiovascular diseases. OBJECTIVES: sL-selectin is an adhesion molecule released from the surface of leukocytes as they are activated and may inhibit leukocyte attachment to the endothelium. The aim of this study was to evaluate sL-selectin serum levels in OSA patients with cardiovascular diseases. MATERIAL AND METHODS: A total of 163 OSA patients were enrolled in the study...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28771237/mechanical-isolation-and-measurement-of-force-and-myoplasmic-free-ca-2-in-fully-intact-single-skeletal-muscle-fibers
#11
Arthur J Cheng, Håkan Westerblad
Mechanical dissection of single intact mammalian skeletal muscle fibers permits real-time measurement of intracellular properties and contractile function of living fibers. A major advantage of mechanical over enzymatic fiber dissociation is that single fibers can be isolated with their tendons remaining attached, which allows contractile forces (in the normal expected range of 300-450 kN/m(2)) to be measured during electrical stimulation. Furthermore, the sarcolemma of single fibers remains fully intact after mechanical dissection, and hence the living fibers can be studied with intact intracellular milieu and normal function and metabolic properties, as well as ionic control...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28752493/mindfulness-meditation-for-fibromyalgia-mechanistic-and-clinical-considerations
#12
REVIEW
Adrienne L Adler-Neal, Fadel Zeidan
PURPOSE OF REVIEW: Fibromyalgia is a disorder characterized by widespread pain and a spectrum of psychological comorbidities, rendering treatment difficult and often a financial burden. Fibromyalgia is a complicated chronic pain condition that requires a multimodal therapeutic approach to optimize treatment efficacy. Thus, it has been postulated that mind-body techniques may prove fruitful in treating fibromyalgia. Mindfulness meditation, a behavioral technique premised on non-reactive sensory awareness, attenuates pain and improves mental health outcomes...
September 2017: Current Rheumatology Reports
https://www.readbyqxmd.com/read/28750339/differential-neuronal-and-glial-behavior-on-flat-and-micro-patterned-chitosan-films
#13
Marta Mattotti, Zaida Alvarez, Luis Delgado, Miguel A Mateos-Timoneda, Conrado Aparicio, Josep A Planell, Soledad Alcántara, Elisabeth Engel
Chitosan is a biodegradable natural polysaccharide that has been widely studied for regenerative purposes in the central nervous system. In this study we assessed the in vitro glial and neuronal cells response to chitosan either flat or patterned with grooves in the micrometric range. Chitosan demonstrated to be a good substrate for the attachment and growth of both neurons and glial cells. Chitosan micropatterns promoted glial cell maturation, suggesting astroglial activation. Nevertheless, those mature/reactive glial cells were permissive for axonal growth...
July 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28746320/c-reactive-protein-in-periodontitis-and-its-comparison-with-body-mass-index-and-smoking-behaviour
#14
S Gupta, S Pradhan, S Kc, S Shakya, M Giri
INTRODUCTION: Chronic periodontitis is an infectious disease resulting in inflammation within the supporting tissues of the teeth, progressive attachment loss, and bone loss. In addition to declining oral health, there is always low grade infection present in periodontitis. Studies show increased levels of systemic biomarkers in periodontal disease such as CRP, which is considered a key-marker of CVD. Research has also shown positive association between BMI and smoking with periodontitis and CRP...
April 2017: JNMA; Journal of the Nepal Medical Association
https://www.readbyqxmd.com/read/28745494/surface-anchored-thiol-reactive-soft-interfaces-engineering-effective-platforms-for-biomolecular-immobilization-and-sensing
#15
Tugce Nihal Gevrek, Irem Kosif, Amitav Sanyal
Fabrication of anti-biofouling, yet specifically reactive polymeric coatings which undergo facile functionalization with thiol-bearing small molecules and ligands yield effective platforms for biomolecular immobilization and sensing. Poly(ethylene glycol) (PEG) based copolymers containing alkoxysilyl groups to enable surface-anchoring and furan-protected maleimide groups as latent thiol-reactive moieties as side-chains were synthesized. Reactive interfaces were obtained by coating these copolymers onto Si/SiO2 or glass surfaces and activating the maleimide groups to their thiol-reactive forms via thermal treatment...
July 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28743084/graphene-oxide-gold-nanorods-based-multiple-assisted-electrochemiluminescence-signal-amplification-strategy-for-sensitive-detection-of-prostate-specific-antigen
#16
Jun-Tao Cao, Jiu-Jun Yang, Li-Zhen Zhao, Yu-Ling Wang, Hui Wang, Yan-Ming Liu, Shu-Hui Ma
A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification...
July 20, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28719005/quantitative-structure-reactivity-relationships-of-hydroxyl-radical-rate-constants-for-linear-and-cyclic-volatile-methylsiloxanes
#17
Jaeshin Kim, Shihe Xu
The accurate understanding of the fate of volatile methylsiloxanes (VMS) in air is crucial in determining persistence and concentrations in the environment. Although oxidation by atmospheric hydroxyl radicals (oOH) is considered as a major degradation mechanism for airborne VMS, the existing bimolecular rate constants with •OH measured and modeled for any given VMS compound varied greatly, depending on the approaches used to generate the data. The objectives of this study were to measure •OH reaction rate constants for four cyclic and four linear VMS based on a relative rate method using a newly designed atmospheric chamber and to establish structure-reactivity relationships for the kinetics...
July 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28715773/developmental-toxicity-of-fe3o4-nanoparticles-on-cysts-and-three-larval-stages-of-artemia-salina
#18
Song Zhu, Ming-Yang Xue, Fei Luo, Wei-Chao Chen, Bin Zhu, Gao-Xue Wang
Using Artemia salina cysts (capsulated and decapsulated) and larvae (instar I, II and III) as experimental models, the potential effects of Fe3O4 nanoparticles (Fe3O4-NPs) on marine ecosystems were investigated. Hatchability, mortality and a number of ethological, morphological and biochemical parameters were selected as end-points to define the toxic responses. Data showed that the hatching rates of capsulated and decapsulated cysts were significantly decreased (p < 0.01) following exposure to 600 mg/L for 24 and 36 h...
July 14, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28715704/oxytocin-receptor-gene-polymorphisms-attachment-and-ptsd-results-from-the-national-health-and-resilience-in-veterans-study
#19
Lauren M Sippel, Shizhong Han, Laura E Watkins, Ilan Harpaz-Rotem, Steven M Southwick, John H Krystal, Miranda Olff, Richard Sherva, Lindsay A Farrer, Henry R Kranzler, Joel Gelernter, Robert H Pietrzak
The human oxytocin system is implicated in social behavior and stress recovery. Polymorphisms in the oxytocin receptor gene (OXTR) may interact with attachment style to predict stress-related psychopathology like posttraumatic stress disorder (PTSD). The objective of this study was to examine independent and interactive effects of the OXTR single nucleotide polymorphism (SNP) rs53576, which has been associated with stress reactivity, support-seeking, and PTSD in prior studies, and attachment style on risk for PTSD in a nationally representative sample of 2163 European-American (EA) U...
July 8, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28712489/plant-made-virus-like-particles-bearing-influenza-hemagglutinin-ha-recapitulate-early-interactions-of-native-influenza-virions-with-human-monocytes-macrophages
#20
Alexander I Makarkov, Sabrina Chierzi, Stéphane Pillet, Keith K Murai, Nathalie Landry, Brian J Ward
INTRODUCTION: Plant-made virus-like particles (VLP) bearing influenza virus hemagglutinins (HA) are novel vaccine candidates that induce cross-reactive humoral and poly-functional T cell responses. To better understand the mechanisms that underlie this broad immunogenicity we studied early interactions of VLPs bearing either H1 (A/California/07/2009 (H1N1)) or H5 (A/Indonesia/05/2005 (H5N1)) with a human monocytoid cell line (U-937 cells) and human monocyte-derived macrophages (MDMs) as model antigen-presenting cells (APC)...
July 13, 2017: Vaccine
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