journal
MENU ▼
Read by QxMD icon Read
search

3 Biotech

journal
https://www.readbyqxmd.com/read/28735432/eucalyptol-sabinene-and-cinnamaldehyde-potent-inhibitors-of-salmonella-target-protein-l-asparaginase
#1
Archana Vimal, Dharm Pal, Timir Tripathi, Awanish Kumar
Salmonella typhimurium is a severe threat to human life. The treatment became more difficult with the emergence of multidrug resistance. In the present in silico study, a novel drug target L-asparaginase was tested against three ligands eucalyptol, sabinene, and cinnamaldehyde, major components of cardamom, nutmeg, and cinnamon, respectively. The lowest docking score was obtained for sabinene followed by eucalyptol and cinnamaldehyde i.e. -5.648, -3.939 and -3.469. The docking score of sabinene is also better than the standard drug, Ciprofloxacin (-4...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28733938/molecular-cloning-heterologous-expression-and-functional-characterization-of-a-cellulolytic-enzyme-cel-prii-from-buffalo-rumen-metagenome
#2
Ravi K Shah, Amrutlal K Patel, Deepti M Davla, Ishan K Parikh, Ramalingam B Subramanian, Kamlesh C Patel, Subhash J Jakhesara, Chaitanya G Joshi
A cellulase encoding gene, Cel PRII, was identified from Mehsani buffalo rumen metagenome, and cloned and expressed in Escherichia coli BL21(DE3)pLysS. The 1170 bp full length gene encodes a 389 residue polypeptide (Cel PRII) containing a catalytic domain belonging to glycosyl hydrolase (GH) 5 family. The fusion protein consisting of the Cel PRII, thioredoxin tag and 6x Histidine tag with predicted molecular weight of 63 kDa when recovered from inclusion bodies under denaturing conditions, exhibited cellulolytic activity against carboxymethyl cellulose (CMC)...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28730551/pseudomonas-fluorescens-r68-assisted-enhancement-in-growth-and-fertilizer-utilization-of-amaranthus-tricolor-l
#3
C Jimtha John, P Jishma, N R Karthika, K S Nidheesh, J G Ray, Jyothis Mathew, E K Radhakrishnan
Plant probiotic potential of rhizosphere microbiome and its role in phytofertilizer mobilization are largely unexplored. In the current study, the rhizobacterium Pseudomonas fluorescens R68 (PFR68) isolated from Western Ghat was analyzed for its growth enhancement effect on the leafy vegetable Amaranthus tricolor (L.). One month of field growth of PFR68 inoculated A. tricolor has found to have enhanced growth parameters such as leaf number (1.57 fold), root number (1.76 fold), shoot length (1.28 fold) and fresh weight (2...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28730550/disease-management-of-tomato-through-pgpb-current-trends-and-future-perspective
#4
REVIEW
Vipin Kumar Singh, Amit Kishore Singh, Ajay Kumar
Tomato is the world's second most cultivated vegetable. During cultivation or post-harvest storage, it is susceptible to more than 200 diseases caused by an array of pathogenic fungi, nematodes, bacteria, and viruses. Although wide range of chemical pesticides are currently available to manage plant diseases, continuous application of pesticides not only affect the nutritional contents of tomato but also the texture or productivity of soil. In this context, plant growth promoting bacteria (PGPB) are one of the nature friendly, safe, and effective alternatives for the management of diseases and pathogens of tomato...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28726218/process-optimization-of-biogas-energy-production-from-cow-dung-with-alkali-pre-treated-coffee-pulp
#5
T Selvankumar, C Sudhakar, M Govindaraju, K Selvam, V Aroulmoji, N Sivakumar, M Govarthanan
Biogas production from cow dung with co-substrate agricultural waste is one of the most demanding technologies for generating energy in a sustainable approach considering eco-friendly. In the present study, coffee pulp (CP) was pre-treated with 1% NaOH and combined with various proportions of cow dung (CD) to explore its biogas producing potentiality. The optimization of the process was studied using Response surface methodology. Statistics based on 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the biogas yield and other parameters...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28721681/genetic-characterization-and-disease-mechanism-of-retinitis-pigmentosa-current-scenario
#6
REVIEW
Muhammad Umar Ali, Muhammad Saif Ur Rahman, Jiang Cao, Ping Xi Yuan
Retinitis pigmentosa is a group of genetically transmitted disorders affecting 1 in 3000-8000 individual people worldwide ultimately affecting the quality of life. Retinitis pigmentosa is characterized as a heterogeneous genetic disorder which leads by progressive devolution of the retina leading to a progressive visual loss. It can occur in syndromic (with Usher syndrome and Bardet-Biedl syndrome) as well as non-syndromic nature. The mode of inheritance can be X-linked, autosomal dominant or autosomal recessive manner...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28721680/evaluation-of-haplotype-diversity-of-achatina-fulica-lissachatina-bowdich-from-indian-sub-continent-by-means-of-16s-rdna-sequence-and-its-phylogenetic-relationships-with-other-global-populations
#7
Vijaya Sai Ayyagari, Krupanidhi Sreerama
Achatina fulica (Lissachatina fulica) is one of the most invasive species found across the globe causing a significant damage to crops, vegetables, and horticultural plants. This terrestrial snail is native to east Africa and spread to different parts of the world by introductions. India, a hot spot for biodiversity of several endemic gastropods, has witnessed an outburst of this snail population in several parts of the country posing a serious threat to crop loss and also to human health. With an objective to evaluate the genetic diversity of this snail, we have sampled this snail from different parts of India and analyzed its haplotype diversity by means of 16S rDNA sequence information...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28721679/phytochemicals-as-potential-antidotes-for-targeting-nf-%C3%AE%C2%BAb-in-rheumatoid-arthritis
#8
REVIEW
R Kowshik Aravilli, S Laveen Vikram, V Kohila
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune destructive arthropathy prevalent among people in the age group of 40-70 years. RA induces severe pain, swelling and stiffness of joints resulting in bone damage. RA leads to reduced life expectancy when left untreated. RA is characterized by synovial hyperplasia, infiltration of inflammatory cells resulting in formation of pannus. Synovial hyperplasia is mediated by proinflammatory cytokines, notably IL-1 and TNF-α. NF-κB is a predominant transcription factor in amplifying the inflammatory response...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28718097/regulatory-genes-and-their-roles-for-improvement-of-antibiotic-biosynthesis-in-streptomyces
#9
REVIEW
Fengjuan Lu, Yanyan Hou, Heming Zhang, Yiwen Chu, Haiyang Xia, Yongqiang Tian
The numerous secondary metabolites in Streptomyces spp. are crucial for various applications. For example, cephamycin C is used as an antibiotic, and avermectin is used as an insecticide. Specifically, antibiotic yield is closely related to many factors, such as the external environment, nutrition (including nitrogen and carbon sources), biosynthetic efficiency and the regulatory mechanisms in producing strains. There are various types of regulatory genes that work in different ways, such as pleiotropic (or global) regulatory genes, cluster-situated regulators, which are also called pathway-specific regulatory genes, and many other regulators...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28714045/characterization-drug-loading-and-antibacterial-activity-of-nanohydroxyapatite-polycaprolactone-nha-pcl-electrospun-membrane
#10
Mohd Izzat Hassan, Naznin Sultana
Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28711983/xanthan-gum-production-using-jackfruit-seed-powder-based-medium-optimization-and-characterization
#11
R Felicia Katherine, C Muthukumaran, G Sharmila, N Manoj Kumar, K Tamilarasan, R Jaiganesh
Xanthan gum (XG) production by Xanthomonas campestris NCIM 2961 using jackfruit seed powder (JSP) as a novel substrate was reported. Central composite design (CCD) of response surface method (RSM) was used to evaluate the linear and interaction effects of five medium variables (JSP, peptone, citric acid, K2HPO4 and KH2PO4) for XG production. Maximum XG production (51.62 g/L) was observed at the optimum level of JSP (4 g/L), peptone (0.93 g/L), citric acid (0.26 g/L), K2HPO4 (1.29 g/L) and KH2PO4 (0.5 g/L)...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28711982/correlation-between-the-methylation-of-the-fut1-promoter-region-and-fut1-expression-in-the-duodenum-of-piglets-from-newborn-to-weaning
#12
Chaohui Dai, Li Sun, Riwei Xia, Shouyong Sun, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Alpha-(1,2)-fucosyltransferase (FUT1) gene has some influence on economically important traits and disease resistance. DNA methylation plays an important role in human diseases but is relatively poorly studied in pigs by regulating the mRNA expression of genes. The aim of this study was to analyze the influence of promoter methylation on the expression of FUT1 gene. We used bisulfite sequencing PCR (BSP) and qPCR to analyze the methylation of the FUT1 5'-flanking region and FUT1 mRNA expression in the duodenum of Sutai piglets from newborn to weaning...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28711981/universal-direct-pcr-amplification-system-a-time-and-cost-effective-tool-for-high-throughput-applications
#13
Anis Ben-Amar, Souheib Oueslati, Ahmed Mliki
Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28710744/cloning-and-characterization-of-f3pyc-gene-encoding-pyruvate-carboxylase-in-aspergillus-flavus-strain-f3
#14
Sadia Qayyum, Ibrar Khan, Zulfiqar Ahmad Bhatti, Changsheng Peng
Pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and L-malic acid. These organic acids play very important role for biological remediation of heavy metals. In this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (F3PYC) from heavy metal resistant indigenous fungal isolate Aspergillus flavus (F3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a calculated molecular weight of 131...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28710743/prevalence-of-tem-shv-and-ctx-m-genes-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-strains-isolated-from-urinary-tract-infections-in-adults
#15
Jayanti Jena, Rajesh Kumar Sahoo, Nagen Kumar Debata, Enketeswara Subudhi
Urinary tract infections (UTIs) are one of the major sources of widespread infectious diseases in the community as well as in the hospitals which increase the cause of morbidity and mortality. Prevalence of extended-spectrum-β-lactamase (ESBL)-producing uropathogenic E. coli isolates has been found to be increased rapidly across the world. The present study was undertaken to find out the frequency of bla TEM, bla CTX-M, and bla SHV genes among E. coli isolates from UTI and detect their sensitivity pattern...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28707276/microalgal-cultivation-for-value-added-products-a-critical-enviro-economical-assessment
#16
REVIEW
Richa Kothari, Arya Pandey, Shamshad Ahmad, Ashwani Kumar, Vinayak V Pathak, V V Tyagi
The present review focuses on the cultivation of algal biomass for generating value-added products (VAP) and to assess their economic benefits and harmful environmental impact. Additionally, the impact of bioreactor designs on the yield of microalgal biomass for VAP is also considered. All these factors are discussed in relation to the impact of microalgae production on the bio-economy sector of commercial biotechnology.
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28707275/vanilla-vanilla-planifolia-jacks-cell-suspension-cultures-establishment-characterization-and-applications
#17
Marco A Ramírez-Mosqueda, Lourdes G Iglesias-Andreu
The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28702939/genetic-diversity-and-relatedness-among-ornamental-purslane-portulaca-l-accessions-unraveled-by-srap-markers
#18
Sizhen Jia, Zhiming Yan, Yuanhua Wang, Yue Wei, Zhenqiang Xie, Fei Zhang
Ornamental purslanes (Portulaca L.) are a popular annual bedding and container plant for landscaping. Little information is available concerning the genetic characterization of ornamental purslane resources thus far. The purpose of this study was to investigate the genetic diversity and relationships present in a collection of ornamental purslanes from Portulaca umbraticola and P. grandiflora cultivated in China, using sequence-related amplified polymorphism (SRAP) markers. The genotyping showed that 16 SRAP primer combinations totally produced 261 informative fragments and averaged 16...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28702938/evaluation-of-fermentation-kinetics-of-acid-treated-corn-cob-hydrolysate-for-xylose-fermentation-in-the-presence-of-acetic-acid-by-pichia-stipitis
#19
Mohan Kashid, Anand Ghosalkar
The efficient utilization of lignocellulosic biomass for ethanol production depends on the fermentability of the biomass hydrolysate obtained after pretreatment. In this work we evaluated the kinetics of ethanol production from xylose using Pichia stipitis in acid-treated corn cob hydrolysate. Acetic acid is one of the main inhibitors in corn cob hydrolysate that negatively impacts kinetics of xylose fermentation by P. stipitis. Unstructured kinetic model has been formulated that describes cell mass growth and ethanol production as a function of xylose, oxygen, ethanol, and acetic acid concentration...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28702937/genetic-engineering-strategies-for-biotic-and-abiotic-stress-tolerance-and-quality-enhancement-in-horticultural-crops-a-comprehensive-review
#20
REVIEW
Nehanjali Parmar, Kunwar Harendra Singh, Deepika Sharma, Lal Singh, Pankaj Kumar, J Nanjundan, Yasin Jeshima Khan, Devendra Kumar Chauhan, Ajay Kumar Thakur
Genetic engineering technique offers myriads of applications in improvement of horticultural crops for biotic and abiotic stress tolerance, and produce quality enhancement. During last two decades, a large number of transgenic horticultural crops has been developed and more are underway. A number of genes including natural and synthetic Cry genes, protease inhibitors, trypsin inhibitors and cystatin genes have been used to incorporate insect and nematode resistance. For providing protection against fungal and bacterial diseases, various genes like chitinase, glucanase, osmotin, defensin and pathogenesis-related genes are being transferred to many horticultural crops world over...
August 2017: 3 Biotech
journal
journal
43684
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"