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3 Biotech

Chen Liu, Xianxian Liu, Wenling Xu, Weimin Fu, Fengde Wang, Jianwei Gao, Qiaoyun Li, Zhigang Zhang, Jingjuan Li, Shufen Wang
High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small RNA libraries and one degradome library. A total of 518 known and 976 novel miRNAs were identified, of which, 338 known and 18 novel miRNAs were expressed in all six libraries, respectively. A total of 52 known and 57 novel miRNAs were identified to be significantly differentially expressed between RE and RL, and 195 mRNAs were verified to be the targets of 194 miRNAs by degradome sequencing...
July 2018: 3 Biotech
Jian-Feng Huang, Bo Zhang, Zhen-Yang Shen, Zhi-Qiang Liu, Yu-Guo Zheng
O -succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli ∆JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain E...
July 2018: 3 Biotech
Arunagiri Kamala, Sushil Kumar Middha, Chandrakant S Karigar
The nutgrass, Cyperus rotundus L. (Family: Cyperaceae), is a colonial, perennial herb considered to have originated in India 2000 years ago and widely used in Ayurveda to treat several ailments. In addition to its prehistoric uses, it is used in several systems of medicine for treating variety of diseases. The synergistic actions of the Cyperus ' compounds have added advantage over that of a single constituent. In the past decade, numerous studies proved analgesic, anti-allergic, anti-arthritic, anti-candida, anti-cariogenic, anti-convulsant, anti-diarrheal, anti-emetic, anti-helminthic, anti-histamine, anti-hyperglycemic, anti-hypertensive, anti-inflammatory, anti-malarial, anti-obesity, antioxidant, anti-platelet, anti-pyretic, anti-ulcer, anti-viral, cardioprotective, cytoprotective, cytotoxic, gastroprotective, hepatoprotective, neuroprotective, ovicidal, and larvicidal, wound healing and inhibition of brain Na+ K+ ATPase activities of C...
July 2018: 3 Biotech
Jing Zhang, Jing Chen, Ruimin Jia, Zhihen Dun, Baotong Wang, Xiaopin Hu, Yang Wang
Three Bacillus amyloliquefaciens isolates (HK1, GSDM02, and GSDM15) were tested for effectiveness in biodegradation of plastic films. Isolates were screened by plate on carbon-free medium and by using the clear-zone formation test. Their biodegradation ability was analyzed based on: film weight reduction, pH change of the fluid medium, a soil microbial biomass carbon test, scanning electron microscopy (SEM), and Fourier transform infrared spectrometry (FTIR). Polyvinyl alcohol (PVA) clear-zone and film weight reduction results revealed that the strain with a bigger clear-zone had a better biodegradation effect, that PVA can be evenly distributed in the medium, and that PVA can be a substitution for polyethylene in screening the biodegradation of strains...
July 2018: 3 Biotech
C M Mehta, Ramesh N Pudake, Rashmi Srivastava, Uma Palni, Anil K Sharma
The present study was carried out to develop a PCR-based molecular marker suitable for screening of disease-suppressive composts against Fusarium wilt of tomato. An effective uncultured bacterial community was screened from our previous study on investigation of microbial communities in composts for their potential for biocontrol of Fusarium wilt. Based on available sequence information (Accession no. HQ388491) of selective community, PCR-based molecular markers were designed and tested for their specificity in different compost sample...
July 2018: 3 Biotech
Lifeng Ping, Fengmei Chen, Fengjie Cui, Wanjun Hu, Wenjing Sun, Na Li, Yan Yang
In this paper, the effects of erythorbic acid (EA) treatment with different concentrations on the quality of Grifola frondosa fruiting bodies stored at 4 °C for 27 days were studied by determining the changes in moisture content, weight loss, browning, electrolyte leakage, malondialdehyde (MDA), and nutritional compounds. The activities of polyphenoloxidase (PPO), cellulase and other antioxidant enzymes including superoxide dismutase (SOD), catalase, and peroxidase (POD) were also measured. Results showed that 0...
July 2018: 3 Biotech
Madhu Choudhary, Parbodh C Sharma, Hanuman S Jat, Abhinandita Dash, Balaji Rajashekar, Andrew J McDonald, Mangi L Jat
In Indo-Gangetic plains (IGP) of India, natural resources (soil, water, and environment) are degrading under the conventional-till (CT)-based management practices in rice-wheat cropping system. A long-term field experiment was conducted to understand the soil bacterial diversity and abundance under different sets of management scenarios (Sc). The study comprised of four scenarios, namely, -Sc.I CT-based rice-wheat system (farmers' practice); Sc.II, partial conservation agriculture (CA) based in which rice is under CT-wheat and mungbean under zero-tillage (ZT); Sc...
July 2018: 3 Biotech
Shi Wang, Shenglan Tian, Mingzhe Li, Zhichao Li
The present study investigated the anti-arthritic effects of methionine in neonatal rats. Rats were divided into four groups, with six rats in each group. The rats were administered methionine (150- or 300-mg/kg body weight) orally for 45 consecutive days. The expression levels of catalase, superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, glutathione peroxidase (Gpx), prostaglandin E2 (PGE2 ), matrix metalloproteinase-3, uric acid, nitric oxide (NO), ceruloplasmin, inducible nitric oxide synthase (iNOS), and nuclear factor (NF)-κB were determined in rheumatoid arthritis-induced neonatal rats...
July 2018: 3 Biotech
Chunjuan Fang, Xiaoyan Chen
The biocontrol function of the repressor of cellulase expression I (ACE1) in Trichoderma atroviride was verified through constructing Δ ace1 mutant strain by Agrobacterium tumefaciens -mediated transformation. The activities of cell wall-degrading enzymes (cellulase, xylanase, chitinase, β-1,3-glucanase, and protease) in the supernatant of Δ ace1 mutant strain were distinctly higher than those of control strain, followed with the elevation of related genes transcript levels. Besides, the Δ ace1 mutant resulted in an elevating transcript level of xyr1 , but no obvious change in the expression of cre1 , which suggested that ACE1 was negative regulator of the xyr1 transcription, but not involved in cre1 transcription...
July 2018: 3 Biotech
Mária Martinovičová, Štefan Janeček
Glycoside hydrolases (GHs) have been classified in the CAZy database into 153 GH families. Currently, there might be four α-amylase families: the main family GH13, the family GH57 with related GH119 and, eventually, also GH126. The family GH57 was established in 1996 as the second and smaller α-amylase family. In addition to α-amylase, it contains 4-α-glucanotransferase, α-glucan branching enzyme, amylopullulanase, dual-specificity amylopullulanase-cyclomaltodextrinase, non-specified amylase, maltogenic amylase and α-galactosidase...
July 2018: 3 Biotech
Vempalli Sudharsan Varma, Kondusamy Dhamodharan, Ajay S Kalamdhad
Bacterial diversity during in-vessel (rotary drum) composting of agricultural waste was characterized using NGS-based 16S rRNA sequencing for microbial identification. The activity of the bacteria was observed to vary with the composting materials and degradation pattern. Taxonomic hits distribution at domain level revealed that 89.5% sequences belonged to bacteria, 9% to eukaryota followed by 1.4% archaea during drum composting. The lowest common ancestor (LCA) classification plot showed the high abundance of the phylum proteobacteria followed by actinobacteria in compost sample...
July 2018: 3 Biotech
Joeng-Ho Lee, Palanivel Velmurugan, Jung-Hee Park, Woo-Suk Chang, Yool-Jin Park, Byung-Taek Oh
The purpose of this study was to examine if fermentation of purple sweet potato ( Ipomoea batatas L.) powder (PSP) by Lactobacillus brevis under green, red, blue, white light-emitting diode (LED) illumination or sunlight might yield functionalized products with good antibacterial, antioxidant activity, and/or cytotoxic activity. The Purple sweet potato (PSP) powder fermented with probiotic bacteria L. brevis under white LED light (1.9 ± 1.80/1.6 ± 0.52), blue LED light (1.4 ± 1.32/1.8 ± 0...
July 2018: 3 Biotech
Pankaj Kumar, Geetika Gambhir, Ayesh Gaur, Krishan C Sharma, Ajay K Thakur, Dinesh K Srivastava
Transgenic broccoli ( Brassica oleracea L. var. italica) cv. 'Solan Green Head' has been developed by using Agrobacterium tumefaciens strain harboring cryIAa gene for resistance against diamondback moth ( Plutella xylostella ). By combining the best treatments of 72 h pre-culturing and 48 h co-cultivation time period, a transformation frequency of 4.92 and 13.83% was obtained from cotyledon and hypocotyl explants, respectively. Supplementing the co-cultivation medium with acetosyringone in a concentration of 100 µM enhanced the transformation frequency to 17...
July 2018: 3 Biotech
Wendy Ancona-Escalante, Raul Tapia-Tussell, Luis Pool-Yam, Abraham Can-Cauich, Gabriel Lizama-Uc, Sara Solís-Pereira
Lignin is a source for obtaining natural phenols with high commercial value that can act as redox mediators enhancing effects in dye decolorization. In this study Trametes hirsuta Bm-2 was grown on wheat bran to produce laccases and phenol extracts (PE). Ultrafiltered phenols obtained at different times were evaluated in their potential as redox mediators of laccase activity and indigo carmin decolorization. Laccase activity (L) on ABTS increased up to 12.4 times with L/PE72 compared with laccase alone and L/PE48 showed the highest level of dye decolorization (97%) compared with laccase (12%)...
July 2018: 3 Biotech
Hadhemi Ben Cheikh, Sara Domingues, Eduarda Silveira, Yosr Kadri, Natasha Rosário, Maha Mastouri, Gabriela Jorge Da Silva
The aim of this study was to identify the carbapenemases from clinical carbapenem-resistant Acinetobacter baumannii-calcoaceticus complex (CRABC) isolates and to assess their potential dissemination by conjugation and natural transformation. CRABC ( n  = 101) were collected consecutively from inpatients of the University Hospital of Monastir, Tunisia, from 2013 to 2016. Antimicrobial susceptibility was determined by the disk diffusion method and E-test. Carbapenemase-encoding genes were screened by PCR...
July 2018: 3 Biotech
Zahra Soleymanpour, Maryam Nikzad, Farid Talebnia, Vahid Niknezhad
Xanthan gum is an exo-polysaccharide industrially produced by fermentation using simple sugars. In this study, broomcorn stem was introduced as a low-cost- and widely available carbon source for xanthan gum fermentation. Broomcorn stem was hydrolyzed using sulphuric acid to liberate reducing sugar which was then used as a carbon source for biosynthesis of xanthan gum by Xanthomonas campesteris . Effects of hydrolysis time (15, 30, 45 and 60 min), sulphuric acid concentration (2, 4, 6 and 8% v/v) and solid loading (3, 4, 5 and 6% w/v) on the yield of reducing sugar and consequent xanthan production were investigated...
July 2018: 3 Biotech
Seyyed Alireza Esmailzadeh Hosseini, Mohammad Salehi, Ghobad Babaie, Assunta Bertaccini
Calendula officinalis plants with phyllody symptoms (CaoP) were observed in Yazd and Ashkezar (Yazd province, Iran) during 2013-2016. Twenty-one symptomatic and four asymptomatic plants were transferred individually to the greenhouse and potted for the biological and molecular characterization of associated phytoplasma. The dodder transmission from symptomatic potted marigold plants, induced virescence, phyllody and witches' broom symptoms in periwinkle. Total DNAs extracted from symptomatic and symptomless plants and dodder-inoculated periwinkles were subjected to nested PCR assay using primer pairs amplifying phytoplasma ribosomal DNA...
July 2018: 3 Biotech
Hui Wang, Kun Jiang, Ziwei Zhu, Wankui Jiang, Zhangong Yang, Shijun Zhu, Jiguo Qiu, Xin Yan, Jian He, Qin He, Qing Hong
Microbial inoculant preparation is a prerequisite for its application in large-scale bioremediation. In the present study, Sphingomonas sp. DC-6, an efficient acetochlor-degrading strain, was used to investigate the process of preparing the inoculant. Optimization of submerged fermentation (SmF) by response surface methodology (RSM) resulted in a first 22% increase in biomass of liquid inoculant. Then, a biomass increase of 2.18 times with 14.58% shortened incubation period was further obtained in optimized medium using a 7...
July 2018: 3 Biotech
Panpan Liu, Yan Zhou, Zhongqi Wu, Hao Zhong, Yanjun Wei, Youzhi Li, Shenkui Liu, Yan Zhang, Xuanjun Fang
Mosquitocidal Bacillus thuringiensis ( Bt ) strain S2160-1 was proposed to be an alternative to Bacillus thuringiensis subsp. israelensis ( Bti ). Discovering and validating a toxic gene by experimentation was a complex and time-consuming task, which can benefit from high-throughput sequencing analysis. In this research, we predicted and identified toxic proteins in the strain S2160-1 based on the draft whole genome sequence data. Through a local BLASP, 46 putative toxins were identified in S2160-1 genome, by searching against a customized B...
July 2018: 3 Biotech
Mónica Costas Malvido, Elisa Alonso González, David Outeiriño, Nelson Pérez Guerra
In this study, probiotic biomass and nisin productions by Lactococcus lactis CECT 539 were followed in two realkalized fed-batch cultures in diluted whey (DW) supplemented with KH2 PO4 up to a total phosphorus concentration of 0.459 g/L. Increased biomass (5.12 g/L, 2.18 × 1010 CFU/mL) and nisin (235.23 BU/mL) concentrations were obtained in the culture fed concentrated whey and concentrated mussel processing waste (CMPW) medium supplemented with glucose up to a concentration of 400 g/L (CMPW + G medium) compared with similar fed-batch fermentations in DW medium...
July 2018: 3 Biotech
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