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3 Biotech

Phatcharin Prayoonthien, Sunee Nitisinprasert, Suttipun Keawsompong
The aim of this study was to carry out preliminary investigations on the in vitro fermentation selectivity of copra meal hydrolysate (CMH) by chicken gut microbiota. The ileum and cecum contents from three 35-day-old birds were used as inocula. Yeast mannooligosaccharide (yeast-MOS) or α-mannan was selected as a positive control. Batch culture fermentation with fecal bacteria was performed at 42 °C for 24 h in an anaerobic chamber. Samples were collected at 0, 6, 12, 18 and 24 h of fermentation and evaluated using real-time PCR and short-chain fatty acid (SCFA) analysis...
January 2018: 3 Biotech
Lopamudra Satapathy, Dhananjay Kumar, Manish Kumar, Kunal Mukhopadhyay
WRKY, a plant-specific transcription factor family, plays vital roles in pathogen defense, abiotic stress, and phytohormone signalling. Little is known about the roles and function of WRKY transcription factors in response to rust diseases in wheat. In the present study, three TaWRKY genes encoding complete protein sequences were cloned. They belonged to class II and III WRKY based on the number of WRKY domains and the pattern of zinc finger structures. Twenty-two DNA-protein binding docking complexes predicted stable interactions of WRKY domain with W-box...
January 2018: 3 Biotech
P Vignesh Kumar, Susheel Kumar Sharma, Narayan Rishi, Virendra Kumar Baranwal
Citrus yellow mosaic virus (CYMV) is a member of genus Badnavirus of the family Caulimoviridae. It is the causal agent of citrus yellow mosaic disease in citrus and causes reduction in yield. As the virus is vegetative propagated by grafting, development of high-throughput diagnosis methods based on serological techniques is a prerequisite for production of healthy virus-free planting material. The current study describes the development of polyclonal antibodies raised in rabbits against purified recombinant virion-associated protein (rVAP) encoded by ORF-II of CYMV...
January 2018: 3 Biotech
Ram Sarup Singh, Kanika Chauhan
A statistical tool of response surface methodology was used sequentially to optimise lactose-based medium and process variables for inulinase production from Penicillium oxalicum BGPUP-4. Two-level CCRD with four variables, for each design, was used for the optimization study. The independent variables: lactose 1-3%, NH4H2PO4 0.2-0.5%, NaNO3 0.2-0.5%, pH 5.0-7.0 (design-1); temperature 25-35 °C, incubation time 4.0-6.0 days, inoculum size 1.0-3.0 mycelial agar discs and agitation 100-200 rpm (design-2) were selected for the present investigation...
January 2018: 3 Biotech
Mandeep Kumari, B N Devanna, Pankaj Kumar Singh, H Rajashekara, Vinay Sharma, Tilak Raj Sharma
The emergence of new strains of Magnaporthe oryzae (M. oryzae) is associated with recurrent failure of resistance response mediated by single resistance (R) gene in rice. Therefore, stacking or combining of multiple R genes could improve the durability of resistance against multiple strains of M. oryzae. To achieve this, in the present study, intragenic stacking of rice blast resistance orthologue genes Pi54 and Pi54rh was performed through co-transformation approach. Both these genes were expressed under the control of independent promoters and blast susceptible indica rice line IET17021 was used for transformation...
January 2018: 3 Biotech
Dharam Pal, Rajan K Tripathy, Madaka Surya Teja, Mukesh Kumar, Uttam Chand Banerjee, Abhay H Pande
Recombinant human interferon-β (rhIFN-β), a therapeutic protein, is produced using both prokaryotic and eukaryotic expression systems. However, instability of recombinant plasmid during cultivation of Escherichia coli results in low yield of the recombinant proteins. In addition, use of antibiotics during the cultivation imposes a major concern. In this study, we have compared the expression yield of rhIFN-β in E. coli BL21 (DE3) and E coli SE1 cells. Gene-encoding rhIFN-β was expressed in E. coli BL21 (DE3) and SE1 cells and the cultivation of recombinant E...
January 2018: 3 Biotech
Antriksh Gupta, Asim Kumar Jana
The objective of the work was to study the effect of agri-residue solid contents (2-20% w v-1) in fermentation medium on fungal growth, soluble and insoluble nutrient consumption and laccase production. Fungal strain Ganoderma lucidium and wheat straw substrate was screened for maximum laccase production. At low solid content submerged fermentation (SmF), fungus utilized mainly soluble nutrient and was unable to access the insoluble nutrient in media due to lack of contact with solid. At high solid content solid-state fermentation (SF), fungi grew on solid surface with dense and thin hyphae, utilized mainly insoluble nutrient...
January 2018: 3 Biotech
Richa Kothari, Shamshad Ahmad, Vinayak V Pathak, Arya Pandey, Saubhagya Singh, Kapil Kumar, V V Tyagi
Wild strains of algal biomass, a major contributor for eutrophication in freshwater bodies, can be used as a potential substrate in association with other nutrient-rich biowaste materials like animal excreta and industrial wastewater, for biogas production. This novel concept was experimentally evaluated and analyzed by the modified Gompertz equation for maximum biogas production (μm), lag phase (λ), and biogas yield (P). The value of correlation coefficient (R2) was 0.99 at varying temperature ranges (30, 40, and 50 °C)...
January 2018: 3 Biotech
Sudipta Roy, Rubia Parvin, Subhadeep Ghosh, Somesankar Bhattacharya, Santanu Maity, Debdulal Banerjee
The tannase production ability by endophytic actinobacteria and the genetic identity of responsible tannase gene were determined. The studied strains were isolated from surface-sterilized leaf discs of Ailanthus excelsa Roxb. Four strains were found to hydrolyze tannic acid on solid media containing 0.4% tannic acid. The strain AL1L was found as tanBLP indicating production of tannase with diverse of substrate affinity. The tannase production from the potential strain AL1L was performed in liquid tannic acid broth (0...
January 2018: 3 Biotech
Vimjam Swarupa, Abhijit Chaudhury, Potukuchi Venkata Gurunadha Krishna Sarma
Staphylococcus aureus plays a major role in persistent infections and many of these species form structured biofilms on different surfaces which is accompanied by changes in gene expression profiles. Further, iron supplementation plays a critical role in the regulation of several protein(s)/enzyme function, which all aid in the development of active bacterial biofilms. It is well known that for each protein, deformylation is the most crucial step in biosynthesis and is catalyzed by peptidyl deformylase (PDF)...
January 2018: 3 Biotech
Kaushik Bhattacharjee, Narasinga Rao Palepu, Kollipara Mohan Rao, Santa Ram Joshi
Natural products or their derivatives provide a reliable resource for new drugs. The multi-step chemical reaction to produce new drug is not only expensive but also release pollutants. The precursor-based combinatorial biosynthesis (PCB) is, however, a better option to produce novel natural products with potential pharmaceutical applications. The present work is an attempt to synthesize an antibacterial compound by transforming thiophene precursor using endolithic Streptomyces sp. AL51. The Streptomyces sp...
January 2018: 3 Biotech
Pilar Escalante-Minakata, Vrani Ibarra-Junquera, José de Jesús Ornelas-Paz, Victoria García-Ibáñez, José J Virgen-Ortíz, Apolinar González-Potes, Jaime D Pérez-Martínez, Mario Orozco-Santos
This work presents a novel method to associate the polyphenol oxidase (PPO) and the peroxidase (POD) activities with the ripening-mediated color changes in banana peel and pulp by computational image analysis. The method was used to follow up the de-greening of peel and browning of homogenized pulp from 'Giant Dwarf' (GD: Musa AAA, subgroup Cavendish) and FHIA-23 (tetraploid hybrid, AAAA) banana cultivars. In both cultivars, the color changes of peel during the ripening process clearly showed four stages, which were used to group the fruit into ripening stages...
January 2018: 3 Biotech
Punit Kumar, Kashyap Kumar Dubey
In the present study, lipstatin production was studied from different mutants of Streptomyces toxytricini which were developed using ultraviolet radiation (exposure time 30 s, 1, 2, 5 and 10 min), ethyl methane sulfonte, methyl methane sulfonate (MMS) and N-methyl-N'-intro-N-nitrosoguanidine (NTG) treatments (50, 100, 200, 500, 1000 µM, respectively). Highest yielding mutants were provided precursor supplementation of citric acid, thiamine and biotin (each 1 g/L) at idiophase for further enhancement in the production of lipstatin...
January 2018: 3 Biotech
Chuannan Long, Jingjing Cui, Hailong Li, Jian Liu, Lihui Gan, Bin Zeng, Minnan Long
The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated β-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in Trichoderma orientalis EU7-22. The binary vector pUR5750G/bxl::hph was constructed to knock out the β-xyl1 gene (encoding β-xylosidases) in T. orientalis EU7-22 by homologous integration, producing the mutant strain T. orientalis Bxyl-1. Xylanase activity for strain Bxyl-1 was 452.42 IU/mL, which increased by only 0...
January 2018: 3 Biotech
Puja Ghosh, Aryadeep Roychoudhury
Accounting for aroma production in different aromatic indica rice varieties based on variations in the levels of concerned metabolites and enzymes is poorly explored. The present investigation was, therefore, focused on unraveling the differential levels of metabolites and activities of enzymes related to aroma formation in eleven indigenous aromatic rice varieties, as compared with four non-aromatic varieties. The levels of metabolites such as proline (Pro) and Δ1-pyrroline-5-carboxylate (P5C), and the activity of related enzymes such as proline dehydrogenase (PDH), Δ1-pyrroline-5-carboxylate synthetase (P5CS), and ornithine aminotransferase (OAT) were comparatively higher in the aromatic varieties, with Kalonunia and Tulaipanji registering the highest Pro, Kalonunia the highest P5C content, Gobindobhog with the highest PDH activity, Gobindobhog and Tulaipanji with the highest P5CS, and Pusa Basmati-1 with the highest OAT activity...
January 2018: 3 Biotech
Rajeswara Reddy Erva, T C Venkateswarulu, Bangaraiah Pagala
Physical and chemical factors influencing the anti-leukemic l-asparaginase enzyme production by Bacillus subtilis VUVD001 were optimized using multi-stage optimization on the basis of preliminary experimental outcomes obtained by conventional one-factor-at-a-time approach using shake flasks. Process variables namely carbon, nitrogen sources, pH and temperature were taken into consideration during response surface methodology (RSM) optimization. The finest enzyme activity of 0.51 IUml-1 obtained by OFAT method was enhanced by 3...
January 2018: 3 Biotech
Junliang Zhong, Xihong Zhao
The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (106 cells/mL) but with no inhibition on viable cells...
January 2018: 3 Biotech
Chander Parkash, Sandeep Kumar, Rajender Singh, Ajay Kumar, Satish Kumar, Shyam Sundar Dey, Reeta Bhatia, Raj Kumar
A comprehensive study on characterization and genetic diversity analysis was carried out in 16 'Ogura'-based 'CMS' lines of cabbage using 14 agro-morphological traits and 29 SSR markers. Agro-morphological characterization depicted considerable variations for different horticultural traits studied. The genotype, ZHA-2, performed better for most of the economically important quantitative traits. Further, gross head weight (0.76), head length (0.60) and head width (0.83) revealed significant positive correlation with net head weight...
January 2018: 3 Biotech
Santosh Kumar Mishra, R K Malik, Harsh Panwar, Amit Kumar Barui
The aim of this study was to microencapsulate the reuterin produced by Lactobacillus reuteri BPL-36 strain for its long-term efficacy against food-borne pathogen Listeria monocytogenes. Lactobacillus reuteri BPL-36 strain previously isolated from a human infant fecal sample in lab was selected for the present study based on its ability to produce reuterin. The organism displayed a broad-spectrum antimicrobial activity. Reuterin concentration of 89.63 mM was obtained in the MRS-glycerol medium after 16 h incubation at 37 °C...
January 2018: 3 Biotech
Jayanthi Abraham, Ritika Chauhan
Actinomycetes strain was isolated from leaf litter soil sample and was identified as Streptomyces sp. by conventional and molecular approaches. The biologically active compound responsible for antimicrobial and anticancer activity of the strain JAR6 was elucidated by solid state fermentation followed by subsequent chromatographic and spectroscopic analysis. Extraction, purification and structural confirmation of red pigment metabolite viz undecylprodigiosin were established on the basis of spectroscopic studies and comparing the data from the literature...
January 2018: 3 Biotech
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