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3 Biotech

Archana Siraree, Nandita Banerjee, Sanjeev Kumar, M S Khan, P K Singh, Sanjeev Kumar, Swati Sharma, R K Singh, Jyotsnendra Singh
Genetic diversity in 92 sugarcane varieties of sub-tropical India was assessed using 30 morphological descriptors and 643 simple sequence repeat (SSR) marker loci. Out of the 30 morphological descriptors, 14 were found polymorphic, and significant variability was recorded for plant height, cane diameter and number of millable canes. Grouping traits like plant growth habit, leaf blade curvature and leaf sheath adherence were found to be predominantly monomorphic. There were a few pairs of varieties (e.g., CoP 9702 and CoP 9302, CoP 9301 and CoSe 01424, UP 05 and Co 1336, CoS 96258 and CoH 110) that showed similar DUS profiles except differing for a few descriptors...
November 2018: 3 Biotech
Young-Han Song, Yong-Gyun Goh, Kyung-Hwan Um, Byung-Sung Park
The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surface of fresh chicken was increased as the number of scalding was increased. Cross contamination of bacteria on carcass surface of fresh chicken was lower in the 0.5% ionized calcium scalding treatment group than that in the control group...
November 2018: 3 Biotech
Simmi P Sreedharan, Avinash Kumar, Parvatam Giridhar
Primers having suboptimal amplification efficiencies were shown to falsely represent fold change expression of the N -methyltransferases gene family involved in caffeine biosynthesis in Coffea canephora . To study this phenomenon, the role of stability of the internal reference gene, as well as the amplification efficiency correction of the primers was investigated. GAPDH and Ubiquitin exhibited a good stability for studying the ontogeny of endosperm tissue, as well as the leaf transcriptome during stress from salicylic acid, methyl jasmonate, PEG-mediated drought and sudden exposure to light...
November 2018: 3 Biotech
Permendra Kumar, Kulandaivelu Mahalingam
Neuronal growth regulator 1 ( NEGR1 ) is a candidate gene for human obesity, which encodes the neural cell adhesion and growth molecule. The aim of the current study was to recognize the non-synonymous SNPs (nsSNPs) with the highest predicted deleterious effect on protein function of the NEGR1 gene. We have used five computational tools, namely, PolyPhen, SIFT, PROVEAN, MutPred and M-CAP, to predict the deleterious and pathogenic nsSNPs of the NEGR1 gene. Homology modeling approach was used to model the native and mutant NEGR1 protein models...
November 2018: 3 Biotech
Zhen-Yu Zhang, Muhammad Fahim Raza, Ziqiang Zheng, Xuhao Zhang, Xinxin Dong, Hongyu Zhang
Bacillus velezensis ZY-1-1 was isolated from the larval gut of the lignocellulose-rich diet-fed scarab beetle, Holotrichia parallela , and confirmed to possess extremely high xylanase (48153.8 ± 412.1 U/L) and relatively moderate cellulase activity (610.1 ± 8.2 U/L). Notably, these xylanase and cellulase activities were enhanced by xylan (1.4 and 5.8-fold, respectively) and cellulose (1.1 and 3.5-fold, respectively), which indicated the hemicellulosic/cellulosic substrate-inducible lignocellulolytic activities of this strain...
November 2018: 3 Biotech
Louis Bengyella, Elsie Laban Yekwa, Sehrish Iftikhar, Kiran Nawaz, Robinson C Jose, Dobgima J Fonmboh, Ernest Tambo, Pranab Roy
The most important insect pests causing severe economic damages to soybean ( Glycine max L.) production worldwide are Chrysodeixis includens ( Walker, Noctuidae ), Anticarsia gemmatalis ( Hübner, Erebidae ), Helicoverpa gelotopoeon ( Dyar, Noctuidae ), Crocidosema aporema ( Walsingham; Tortricidae ), Spodoptera albula (Walker, Noctuidae), S. cosmiodes (Walker, Noctuidae), S. eridania (Stoll, Noctuidae), S. frugiperda (Smith; Noctuidae), Helicoverpa armigera (Hübner, Noctuidae), H. zea (Boddie; Noctuidae) and Telenomus podisi (Hymenoptera,Platygastidae)...
November 2018: 3 Biotech
Hillol Chakdar, Syed G Dastager, Jayant M Khire, Digeshwar Rane, Mahesh S Dharne
Five highly efficient phosphate solubilizing bacteria, viz., Pantoea sp. A3, Pantoea sp. A34, Kosakonia sp. A37, Kosakonia sp. B7 and Bacillus sp. AH9 were isolated from termitorial soils of Sanjivani island of southern Maharashtra, India. These isolates were characterized and explored for phosphate solubilization and plant growth promotion. Among these, Bacillus sp. AH9 showed highest phosphate solubilization index (3.5) and solubilization efficiency (250%) on Pikovskaya agar. Interestingly, Pantoea sp. A34 displayed maximum mineral phosphate solubilization (1072...
November 2018: 3 Biotech
Poonam Vishwakarma, Atanu Banerjee, Ritu Pasrija, Rajendra Prasad, Andrew M Lynn
Major facilitator superfamily is one of the largest superfamily of secondary transporters present across the kingdom of life. Considering the physiological and clinical importance of MFS proteins, we attempted to explore the phylogenetic and structural aspects of the superfamily. To achieve the objectives, we performed global sequence-based analyses of MFS proteins encompassing multiple taxa. Notably, phylogenetic analysis of MFS proteins resulted in the clustering of MFS proteins based on their function, rather than lineage of the respective organisms...
November 2018: 3 Biotech
Huanhuan Jiang, Peishi Qi, Tong Wang, Mian Wang, Mingna Chen, Na Chen, Lijuan Pan, Xiaoyuan Chi
Halotolerant phosphate-solubilizing microorganisms (PSMs) capable of producing plant-growth-promoting traits were grown on salt medium containing Ca3 (PO4 )2 or egg yolk. The number of colonies on plates with Ca3 (PO4 )2 was higher than that on plates with egg yolk. Further, a total of 42 PSM isolates were purified. The majority were Bacillus spp., while one Providencia rettgeri strain was confirmed, for the first time, as a PSM. All PSMs had a phosphate-solubilizing index (PSI) between 1.1 and 2.58 and a strong capacity for dissolving calcium phosphate between 2...
November 2018: 3 Biotech
Anil Kumar, Rajesh Kumar Pathak, Aranyadip Gayen, Supriya Gupta, Manoj Singh, Charu Lata, Himanshu Sharma, Joy Kumar Roy, Sanjay Mohan Gupta
Seeds serve as biochemical factories of nutrition, processing, bio-energy and storage related important bio-molecules and act as a delivery system to transmit the genetic information to the next generation. The research pertaining towards delineating the complex system of regulation of genes and pathways related to seed biology and nutrient partitioning is still under infancy. To understand these, it is important to know the genes and pathway(s) involved in the homeostasis of bio-molecules. In recent past with the advent and advancement of modern tools of genomics and genetic engineering, multi-layered 'omics' approaches and high-throughput platforms are being used to discern the genes and proteins involved in various metabolic, and signaling pathways and their regulations for understanding the molecular genetics of biosynthesis and homeostasis of bio-molecules...
November 2018: 3 Biotech
Abdul Faisal Panichikkal, Priji Prakasan, Unni Kizhakkepowathial Nair, Mohanan Kulangara Valappil
In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. Response Surface Methodology (RSM) cum Box-Behnken Design (BBD) was employed to optimize the combined effect of different independent parameters namely oil-methanol ratio, enzyme unit, reaction temperature and reaction time...
November 2018: 3 Biotech
Snehasish Mishra, Puneet Kumar Singh, Swagatika Dash, Ritesh Pattnaik
Biogas obtained from organic remains entails a developed technology and an appreciable methane yield, but its use may not be sustainable. The potential methane yield of various lignocellulose biomass and the operational conditions employed are inherently reviewed. Although of lower methane yields compared to conventional substrates, agricultural biomass is a cheap option. The major challenges encountered during its biogasification are its recalcitrance nature primarily due to the presence of crystalline cellulose and lignin...
November 2018: 3 Biotech
Michay Diez, Myrian Trotta, Victoria Alfonso, Oscar Taboga, María Gabriela López
Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1-3 , a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G . These carried the sequence of the fusion protein POLH-Δ3AB1-3 with an additional copy in cis of polh or polh E44G , respectively, under p10 promoter...
November 2018: 3 Biotech
Armando Mejía, Doris Luna, Francisco J Fernández, Javier Barrios-González, Luis H Gutierrez, Ana G Reyes, Angel E Absalón, Steven Kelly
Expression of the vhb gene encoding hemoglobin from Vitreoscilla stercoraria in several organisms, clearly enhances oxygen-dependent product formation. In a previous work, we expressed the vhb gene that encodes hemoglobin from V. stercoraria in Amycolatopsis mediterranei , resulting in an increase (oxygen-dependent formation) in rifamycin B production. In the present work, we first confirm; by heterologous expression in Escherichia coli , that rif-orf5 from the rifamycin biosynthetic gene cluster, really encodes a cytochrome P450 enzyme, which is the key step for oxygen incorporation in the final biosynthetic product...
November 2018: 3 Biotech
Shilpi Nagar, Ajay Kumar Shaw, Shalini Anand, S Mary Celin, Pramod Kumar Rai
In this report, aerobic biodegradation of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine or high melting explosive (HMX), a highly explosive chemical by Planomicrobium flavidum strain S5-TSA-19, an isolate from an explosive-contaminated soil, was investigated. The isolate S5-TSA-19 degraded 70% of HMX in 20 days during which time nitrite ion was produced with the subsequent formation of metabolites, viz. methylenedintramine and N -methyl- N,N '-dinitromethanediamine with molecular weights 136 Da and 149 Da, respectively...
November 2018: 3 Biotech
Rajesh Kumar Pathak, Mamta Baunthiyal, Dinesh Pandey, Anil Kumar
With the continuous increase in the population of developing countries and decline of natural resources, there is an urgent need to qualitatively and quantitatively augment crop productivity by using new tools and technologies for improvement of agriculturally important traits. The new scientific and technological omics-based approaches have enabled us to deal with several issues and challenges faced by modern agricultural system and provided us novel opportunities for ensuring food and nutritional security...
November 2018: 3 Biotech
Yosra Habachi-Houimli, Yosra Khalfallah, Maha Mezghani-Khemakhem, Hanem Makni, Mohamed Makni, Dhia Bouktila
In this study, a systematic analysis of Nucleotide-Binding Site (NBS) disease resistance ( R ) gene family in the barley, Hordeum vulgare L. cv. Bowman, genome was performed. Using multiple computational analyses, we could identify 96 regular NBS-encoding genes and characterize them on the bases of structural diversity, conserved protein signatures, genomic distribution, gene duplications, differential expression, selection pressure, codon usage, regulation by microRNAs and phylogenetic relationships. Depending on the presence or absence of CC and LRR domains; the identified NBS genes were assigned to four distinct groups; NBS-LRR (53...
November 2018: 3 Biotech
Zhiwei Zhang, Fenghua Zhang, Liya Song, Ning Sun, Weishi Guan, Bo Liu, Jian Tian, Yuhong Zhang, Wei Zhang
β-Galactosidase is widely used for hydrolysis of whey lactose. However, galactose inhibition has acted as a major constraint on the catalytic process. Thus, it is sensible to improve upon this defect in β-galactosidase through protein modification. To reduce the galactose inhibition of Aspergillus candidus β-galactosidase (LACB), four amino acid positions were selected for mutation based on their molecular bindings with galactose. Four mutant libraries (Tyr96, Asn140, Glu142, and Tyr364) of the LACB were constructed using site-directed mutagenesis...
November 2018: 3 Biotech
Camila Agostini, Camila Eckert, Angélica Vincenzi, Bruna Lenhardt Machado, Bruna Cristina Jordon, João Pedro Kipper, Anja Dullius, Carlos Henrique Dullius, Daniel Neutzling Lehn, Raul Antonio Sperotto, Adriane Pozzobon, Camille Eichelberger Granada, Mônica Jachetti Maciel, Claucia Fernanda Volken de Souza
In this study, we isolated Lactobacillus spp. from bovine raw milk and artisanal cheese from southern Brazil, and evaluated their technological and probiotic potential to select new isolates for producing healthy fermented dairy foods with differentiated tastes and flavours. We obtained 48 new lactobacilli isolates, which were isolated from raw milk (38) and cheese (10). These bacterial isolates were closely related with ten species: Lactobacillus paracasei (50% of the isolates), L. parabuchneri (15%), L. pentosus (13%), L...
November 2018: 3 Biotech
Jiang Wu, Xuedong Ji, Shenhong Tian, Shaoxia Wang, Huarong Liu
To investigate the biological function of zeaxanthin under UV light and oxidative stress we have increased its biosynthesis capacity in tobacco plants ( Nicotiana tabacum cv . SR-1) by transformation with Citrus kinokuni β-carotene hydroxylase gene ( chyb ) under constitutive promoter control. The chyb transformants synthesized zeaxanthin about 30% more than the controls under UV irradiation. For revelation of direct effects, pigment composition, chlorophyll fluorescence, and photosynthesis were detected immediately after UV treatment...
November 2018: 3 Biotech
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