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3 Biotech

Davod Darvishi Zeidabadi, Mokhtar Jalali Javaran, Hamid Dehghani, Sajad Rashidi Monfared, Amin Baghizadeh
Black caraway is of great importance for its terpene compounds. Many genes are involved in the biosynthesis of secondary metabolites in medicinal plants. For this study, black caraway seeds were collected from five different regions, i.e. [Isfahan; Kerman (Khabr); Semnan; Kerman (Sirch); and Hormozgan]. The black caraway seed oil was extracted and analyzed by means of the gas chromatography method. There was a negatively significant correlation ( p  ≤ 0.05) observed between cuminaldehyde and gammaterpinene compounds...
September 2018: 3 Biotech
Roshmi Thomas, S Snigdha, K B Bhavitha, Seethal Babu, Anjitha Ajith, E K Radhakrishnan
In this study, polymethyl methacrylate (PMMA) thin films incorporated with biofabricated silver nanoparticles were used to evaluate the in vitro antimicrobial and antibiofilm activity against the cariogenic bacterium Streptococcus mutans . For this, silver nanoparticles (AgNPs) were generated using Bacillus amyloliquefaciens SJ14 culture (MAgNPs) and extract from Curcuma aromatica rhizome (CAgNPs). The AgNPs were further characterized by UV-Vis spectroscopy and high-resolution transmission electron microscopy...
September 2018: 3 Biotech
Shangde Sun, Jinming Liu, Xulei Li
Fatty acids are the precursors for the production of fuels, oleochemicals and special health care products. In this study, a novel rapid method for fatty acid (FA) preparation by the enzymatic hydrolysis of Phoenix tree seed, an undeveloped woody oil seed, was developed. High-temperature GC with flame ionization detector (FID) and the hydrolysis ratio were used to monitor reaction progress. Enzyme screening and the effect of reaction variables on the hydrolysis of seeds were evaluated and optimized by response surface methodology...
September 2018: 3 Biotech
Pinaki Dey, Joginder Singh, Jismole Scaria, Athira P Anand
Experimental investigations were carried out to develop economic production process of cellulase using coconut mesocarp as an inexpensive lignocellulosic inducer while replacing commercial cellulose. Cellulase production was initially investigated from commercial cellulose in different submerged conditions using Trichoderma reesei (MTCC 164). Maximum enzyme production was achieved 6.3 g/l with activity level 37 FPU/ml in the condition where cellulose to water content ratio was maintained at 5:35 (W/V). To achieve similar maximum production of cellulase from coconut mesocarp, response surface methodology was implemented to optimize most influencing parameters...
September 2018: 3 Biotech
Basista Rabina Sharma, Vikas Kumar, Yogesh Gat, Naveen Kumar, Aarya Parashar, Dave Jaydeep Pinakin
A rapid change in the lifestyle has witnessed poor health with the increased incidences of numerous diseases in the recent years, and ultimately increasing the demand of nutritious foods containing phytochemicals. A wide range of phytochemicals (secondary metabolites) is being synthesized in plants, which influence the human health upon consumption as dietary component. Recently, a number of the technologies (conventional and non-conventional methods) have been standardized by the different researchers for the extraction of these phytochemicals depending upon the raw material...
September 2018: 3 Biotech
Bangchun Wang, Laipeng Luo, Dongmei Wang, Rui Ding, Jiong Hong
Uricase as an important healthcare-related protein is extensively used in the treatment of tumor lysis syndrome and in the manufacture of serum uric-acid diagnostic kits. In this study, a gene of a new thermostable uricase (KmUOX) was cloned from thermotolerant yeast Kluyveromyces marxianus . The uricase was fused with a self-cleaving intein and cellulose-binding affinity tag and expressed in Escherichia coli BL21 (DE3). Through the binding to inexpensive cellulose and in situ intein cleavage induced by a pH change, tag-free uricase (KmUOX) was efficiently purified with a 77...
September 2018: 3 Biotech
Aditi Jain, Vibha Rani
The present study was designed to compare the cardiotoxicity of two very commonly used anti-diabetic drugs namely pioglitazone (Pio) and metformin (Met); and to study the effects of curcumin (Curc) against these drug-induced cardiotoxicity. Curc, being an anti-oxidant molecule and having cardio-protective potential, can have promising synergistic effects in reducing the cardiac stress induced by anti-diabetic therapies. Various dose and time-dependent cell viability and oxidative stress assays were conducted to study cardiotoxic side-effects and Curc-mediated effects in cardiomyoblasts...
September 2018: 3 Biotech
S Pandiyarajan, P Premasudha, K Kadirvelu
In the present study, 40 pigment-producing microbes were isolated from various soil sources. Among these, a novel water-soluble yellow pigment-producing fungal isolate (MBYP1) was identified as Aspergillus sp . through ITS gene sequencing. The maximum pigment yield (UA430nm , 12.45 ± 0.5 g/l) was obtained when strain MBYP1 was cultured under optimum conditions (28 °C and pH 5.5 under static condition). Subsequently, the pigment was purified through gel chromatography and high-performance liquid chromatography (HPLC)...
September 2018: 3 Biotech
Chi Eun Hong, Jang Uk Kim, Jung Woo Lee, Sung Woo Lee, Ick-Hyun Jo
Although endophytic bacteria are known to colonize Panax ginseng , little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total of 116 strains assigned to 42 species were identified by 16S rDNA sequencing. The predominant phylum was Firmicutes . Two-year-old ginseng plants and root tissues showed the greatest diversity of endophytic bacteria, with Bacillales being the predominant order...
September 2018: 3 Biotech
Anna Dotsenko, Alexander Gusakov, Aleksandra Rozhkova, Olga Sinitsyna, Igor Shashkov, Arkady Sinitsyn
Mutant forms of recombinant endoglucanase II (EG II, N194A), cellobiohydrolase I (CBH I, N45A) and cellobiohydrolase II (CBH II, N219A) from Penicillium verruculosum with enhanced cellulase activities, achieved by engineering of enzyme N -glycosylation sites in our previous studies, were used as components of the binary and ternary mixtures of cellulases in hydrolysis of Avicel and milled aspen wood. Using the engineered forms of the enzymes at a dosage of 10 mg/g substrate resulted in significant boosting of the glucose release from cellulose in the presence of excess β-glucosidase relative to the performance of the corresponding wild-type mixtures at the same loading...
September 2018: 3 Biotech
Zaahira Aumeeruddy-Elalfi, Ismaël Saïd Ismaël, Muzzammil Hosenally, Gokhan Zengin, Mohamad Fawzi Mahomoodally
The biofilm inhibition and eradication potential of essential oils (EOs) extracted from six tropical medicinal herbs and food plants [ Psiadia arguta (PA), Psiadia terebinthina (PT), Citrus grandis (CGp), Citrus hystrix (CH), Citrus reticulata (CR), and Cinnamomum zeylanicum (CZ)] were assessed. The mechanism of inhibition was studied via quenching of efflux pump. Cytotoxicity was evaluated using Artemia salina assay and cell lines [human cervix carcinoma (HeLa), human lung fibroblast (MRC-5), and murine melanoma (B16F10)]...
September 2018: 3 Biotech
Jianmin Xu, Zhiming Yan, Zhigang Xu, Yuanhua Wang, Zhenqiang Xie
Light is an important factor for plant development and has serious effects on the growth, production and quality of potatoes. However, the physical and molecular mechanisms by which potato plantlets cope with different light qualities are not understood. In this study, the potato "Zhuanxinwu", which is a germplasm potato resource with a high anthocyanin content, was used for physiological and transcriptome profiling analyses to uncover the different mechanisms that occur in response to blue, red and white light conditions, with the white light condition serving as the control...
September 2018: 3 Biotech
Aung Htay Naing, Da Young Park, Kyeung Il Park, Chang Kil Kim
We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars 'Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both cultivars. Enhancement of anthocyanin content in 'Nathasha' was associated with upregulation of ANS and MYB10, whereas in 'Rosalin', upregulation was associated with CHS1 , MYB10, and MYC1. Low-temperature exposure (6 °C) enhanced anthocyanin content to a greater extent than that at 22 °C via stronger upregulation of CHS1 and MYB10 in 'Nathasha' and CHS1 in 'Rosalin', irrespective of flower developmental stage...
September 2018: 3 Biotech
Yunya Li, Wei Jiang, Ruijie Gao, Yujie Cai, Zhengbing Guan, Xiangru Liao
Catechol siderophore plays an important role in microbial ecology, agriculture, and medicine, but its research is often limited by the difficulty in acquisition of it in large quantities. Based on evidence from the coordination chemistry and chemical biology, catechol siderophore could chelate Fe3+ with high affinity. Therefore, Fe(III)-based immobilized metal-affinity chromatography (IMAC) was applied to capture siderophore from the culture filtrate of Bacillus tequilensis CD36. The ethanol-precipitated sample and the separated sample from Fe(III)-based IMAC were analyzed by liquid chromatography-mass spectrometry...
September 2018: 3 Biotech
Azam Badrhadad, Farhad Nazarian-Firouzabadi, Ahmad Ismaili
An antibacterial peptide-encoding gene from alfalfa seeds, alfAFP, was fused to the C-terminal part of chitin-binding domain (CBD) of the rice chitinase-encoding gene (CBD-alfAFP) and introduced to tobacco by Agrobacterium -mediated transformation. Polymerase chain reaction (PCR) technique was used to confirm the integration of the recombinant CBD-alfAFP encoding gene in transgenic tobacco plants. A number of transgenic lines and a non-transgenic control plant were selected for further molecular analyses. The result of analyzing the transgenic plants by semi-quantitative RT-PCR showed that the recombinant gene is expressed in transgenic plants and there is a difference between the transgenic plants in terms of the level of CBD-alfAFP expression...
September 2018: 3 Biotech
Açelya Yilmazer
In this study, the effect of spheroid formation, as a model of three-dimensional (3D) culture systems, on the cancer stemness of human breast cancer (MCF-7) and human glioma (U87-MG) cell lines was analyzed. We compared the expression of pluripotency genes, the presence of various cancer stem cell populations, migration and proliferation capacities of cells cultured as monolayers or spheroids. MCF-7 cells formed uniform spheroids in vitro, upregulated the expression of stem cell markers both at gene and protein levels and increased their migration capacities when cultured in 3D systems...
September 2018: 3 Biotech
Sajjad Asaf, Abdul Latif Khan, Muhammad Aaqil Khan, Ahmed Al-Harrasi, In-Jung Lee
Our study aimed to elucidate the plant growth-promoting characteristics and the structure and composition of Sphingomonas sp. LK11 genome using the single molecule real-time (SMRT) sequencing technology of Pacific Biosciences. The results revealed that LK11 produces different types of gibberellins (GAs) in pure culture and significantly improves soybean plant growth by influencing endogenous GAs compared with non-inoculated control plants. Detailed genomic analyses revealed that the Sphingomonas sp. LK11 genome consists of a circular chromosome (3...
September 2018: 3 Biotech
Marek Kieliszek, Stanisław Błażejak, Kamil Piwowarek, Katarzyna Brzezicka
The study investigated the effectiveness of selenium binding from its salt solution by Candida utilis ATCC 9950 yeast biomass cultured on a medium prepared from the agro-food industry wastes, containing an available source of carbon and nitrogen. Selenium binding by C. utilis yeast strain after 48 h of culturing at 28 °C from aqueous solutions with the addition of 30 mg Se/L reached a value of 2.28 mg Se/g of yeast biomass. The kinetics of selenium binding by the yeasts showed a better fit for the pseudo-second-order kinetic model compared to the pseudo-first-order one...
September 2018: 3 Biotech
Jinlong Li, Weijia Shen, Guangsen Fan, Xiuting Li
A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia . Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of this lipase was pH 8.0 and 50 °C, respectively...
September 2018: 3 Biotech
Vijaya Gowri Esvaran, Tania Gupta, A R Narasimha Nayaka, Vankadara Sivaprasad, Kangayam M Ponnuvel
Nosema bombycis is a spore-forming parasite causing microsporidiosis in silkworm Bombyx mori . Methionine aminopeptidase 2 ( MetAP2 ), an essential gene of N. bombycis , is a target for the anti-microsporidian drug Fumagillin, an antibiotic derived from Aspergillus fumigatus . In this study, a 1077 bp full-length cDNA of the MetAP2 gene of N. bombycis was cloned and characterized. Furthermore, the expression study of the MetAP2 gene revealed a ubiquitous expression during all the developmental stages of the silkworm B...
September 2018: 3 Biotech
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