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Frontiers in Microbiology

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https://www.readbyqxmd.com/read/29780378/selection-and-validation-of-reference-genes-for-quantitative-real-time-pcr-normalization-under-ethanol-stress-conditions-in-oenococcus-oeni-sd-2a
#1
Shuai Peng, Longxiang Liu, Hongyu Zhao, Hua Wang, Hua Li
The powerful Quantitative real-time PCR (RT-qPCR) was widely used to assess gene expression levels, which requires the optimal reference genes used for normalization. Oenococcus oeni ( O. oeni ), as the one of most important microorganisms in wine industry and the most resistant lactic acid bacteria (LAB) species to ethanol, has not been investigated regarding the selection of stable reference genes for RT-qPCR normalization under ethanol stress conditions. In this study, nine candidate reference genes ( proC, dnaG, rpoA, ldhD, ddlA, rrs, gyrA, gyrB , and dpoIII ) were analyzed to determine the most stable reference genes for RT-qPCR in O...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780377/the-gut-microbiota-of-marine-fish
#2
REVIEW
Sian Egerton, Sarah Culloty, Jason Whooley, Catherine Stanton, R Paul Ross
The body of work relating to the gut microbiota of fish is dwarfed by that on humans and mammals. However, it is a field that has had historical interest and has grown significantly along with the expansion of the aquaculture industry and developments in microbiome research. Research is now moving quickly in this field. Much recent focus has been on nutritional manipulation and modification of the gut microbiota to meet the needs of fish farming, while trying to maintain host health and welfare. However, the diversity amongst fish means that baseline data from wild fish and a clear understanding of the role that specific gut microbiota play is still lacking...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780376/diversity-novelty-and-antimicrobial-activity-of-endophytic-actinobacteria-from-mangrove-plants-in-beilun-estuary-national-nature-reserve-of-guangxi-china
#3
Zhong-Ke Jiang, Li Tuo, Da-Lin Huang, Ilya A Osterman, Anton P Tyurin, Shao-Wei Liu, Dmitry A Lukyanov, Petr V Sergiev, Olga A Dontsova, Vladimir A Korshun, Fei-Na Li, Cheng-Hang Sun
Endophytic actinobacteria are one of the important pharmaceutical resources and well known for producing different types of bioactive substances. Nevertheless, detection of the novelty, diversity, and bioactivity on endophytic actinobacteria isolated from mangrove plants are scarce. In this study, five different mangrove plants, Avicennia marina, Aegiceras corniculatum, Kandelia obovota, Bruguiera gymnorrhiza , and Thespesia populnea , were collected from Beilun Estuary National Nature Reserve in Guangxi Zhuang Autonomous Region, China...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780375/effect-of-artinm-on-human-blood-cells-during-infection-with-paracoccidioides-brasiliensis
#4
Luciana P Ruas, Livia M Genaro, Amauri S Justo-Junior, Lilian O Coser, Lívia F de Castro, Plinio Trabasso, Ronei L Mamoni, Maria-Cristina Roque-Barreira, Maria-Heloisa S L Blotta
Infections caused by fungi are prominent in our environment and can be potentially fatal. paracoccidioidomycosis (PCM), caused by fungi of the Paracoccidioides genus, is the most frequent systemic mycosis in Brazil and the main cause of death among immunocompetent individuals. The antifungal therapy for PCM is usually effective but side effects and relapses are often reported. The latter could be avoided with alternative or complementary therapies aimed at boosting the immune response to combat this pathogen...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780374/insight-into-the-variation-of-bacterial-structure-in-atrazine-contaminated-soil-regulating-by-potential-phytoremediator-pennisetum-americanum-l-k-schum
#5
Bo Cao, Ying Zhang, Ziyi Wang, Mengyuan Li, Feng Yang, Duo Jiang, Zhao Jiang
Although plants of the genus Pennisetum can accelerate the removal of atrazine from its rhizosphere, the roles played by this plant in adjusting the soil environment and soil microorganism properties that might contribute to pollutant removal are incompletely understood. We selected Pennisetum americanum (L.) K. Schum ( P. americanum ) as the test plant and investigated the interaction between P. americanum and atrazine-contaminated soil, focusing on the adjustment of the soil biochemical properties as well as bacterial functional and community diversity in the rhizosphere using Biolog EcoPlates and high-throughput sequencing of the 16S rRNA gene...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780373/regulation-mechanism-mediated-by-trans-encoded-srna-nc117-in-short-chain-alcohols-tolerance-in-synechocystis-sp-pcc-6803
#6
Yanqi Bi, Guangsheng Pei, Tao Sun, Zixi Chen, Lei Chen, Weiwen Zhang
Microbial small RNAs (sRNAs) play essential roles against many stress conditions in cyanobacteria. However, little is known on their regulatory mechanisms on biofuels tolerance. In our previous sRNA analysis, a trans -encoded sRNA Nc117 was found involved in the tolerance to ethanol and 1-butanol in Synechocystis sp. PCC 6803. However, its functional mechanism is yet to be determined. In this study, functional characterization of sRNA Nc117 was performed. Briefly, the exact length of the trans -encoded sRNA Nc117 was determined to be 102 nucleotides using 3' RACE, and the positive regulation of Nc117 on short chain alcohols tolerance was further confirmed...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780372/highly-promiscuous-oxidases-discovered-in-the-bovine-rumen-microbiome
#7
Lisa Ufarté, Gabrielle Potocki-Veronese, Davide Cecchini, Alexandra S Tauzin, Angeline Rizzo, Diego P Morgavi, Bernard Cathala, Céline Moreau, Megane Cleret, Patrick Robe, Christophe Klopp, Elisabeth Laville
The bovine rumen hosts a diverse microbiota, which is highly specialized in the degradation of lignocellulose. Ruminal bacteria, in particular, are well equipped to deconstruct plant cell wall polysaccharides. Nevertheless, their potential role in the breakdown of the lignin network has never been investigated. In this study, we used functional metagenomics to identify bacterial redox enzymes acting on polyaromatic compounds. A new methodology was developed to explore the potential of uncultured microbes to degrade lignin derivatives, namely kraft lignin and lignosulfonate...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780371/comparative-metatranscriptomics-of-wheat-rhizosphere-microbiomes-in-disease-suppressive-and-non-suppressive-soils-for-rhizoctonia-solani-ag8
#8
Helen L Hayden, Keith W Savin, Jenny Wadeson, Vadakattu V S R Gupta, Pauline M Mele
The soilborne fungus Rhizoctonia solani anastomosis group (AG) 8 is a major pathogen of grain crops resulting in substantial production losses. In the absence of resistant cultivars of wheat or barley, a sustainable and enduring method for disease control may lie in the enhancement of biological disease suppression. Evidence of effective biological control of R. solani AG8 through disease suppression has been well documented at our study site in Avon, South Australia. A comparative metatranscriptomic approach was applied to assess the taxonomic and functional characteristics of the rhizosphere microbiome of wheat plants grown in adjacent fields which are suppressive and non-suppressive to the plant pathogen R...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780370/rhizosphere-protists-change-metabolite-profiles-in-zea-mays
#9
Anke Kuppardt, Thomas Fester, Claus Härtig, Antonis Chatzinotas
Plant growth and productivity depend on the interactions of the plant with the associated rhizosphere microbes. Rhizosphere protists play a significant role in this respect: considerable efforts have been made in the past to reveal the impact of protist-bacteria interactions on the remobilization of essential nutrients for plant uptake, or the grazing induced changes on plant-growth promoting bacteria and the root-architecture. However, the metabolic responses of plants to the presence of protists or to protist-bacteria interactions in the rhizosphere have not yet been analyzed...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780369/identification-and-removal-of-contaminant-sequences-from-ribosomal-gene-databases-lessons-from-the-census-of-deep-life
#10
Cody S Sheik, Brandi Kiel Reese, Katrina I Twing, Jason B Sylvan, Sharon L Grim, Matthew O Schrenk, Mitchell L Sogin, Frederick S Colwell
Earth's subsurface environment is one of the largest, yet least studied, biomes on Earth, and many questions remain regarding what microorganisms are indigenous to the subsurface. Through the activity of the Census of Deep Life (CoDL) and the Deep Carbon Observatory, an open access 16S ribosomal RNA gene sequence database from diverse subsurface environments has been compiled. However, due to low quantities of biomass in the deep subsurface, the potential for incorporation of contaminants from reagents used during sample collection, processing, and/or sequencing is high...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780368/-salmonella-enterica-prophage-sequence-profiles-reflect-genome-diversity-and-can-be-used-for-high-discrimination-subtyping
#11
Walid Mottawea, Marc-Olivier Duceppe, Andrée A Dupras, Valentine Usongo, Julie Jeukens, Luca Freschi, Jean-Guillaume Emond-Rheault, Jeremie Hamel, Irena Kukavica-Ibrulj, Brian Boyle, Alexander Gill, Elton Burnett, Eelco Franz, Gitanjali Arya, Joel T Weadge, Samantha Gruenheid, Martin Wiedmann, Hongsheng Huang, France Daigle, Sylvain Moineau, Sadjia Bekal, Roger C Levesque, Lawrence D Goodridge, Dele Ogunremi
Non-typhoidal Salmonella is a leading cause of foodborne illness worldwide. Prompt and accurate identification of the sources of Salmonella responsible for disease outbreaks is crucial to minimize infections and eliminate ongoing sources of contamination. Current subtyping tools including single nucleotide polymorphism (SNP) typing may be inadequate, in some instances, to provide the required discrimination among epidemiologically unrelated Salmonella strains. Prophage genes represent the majority of the accessory genes in bacteria genomes and have potential to be used as high discrimination markers in Salmonella ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780367/expression-of-mir-34a-in-t-cells-infected-by-human-t-lymphotropic-virus-1
#12
Varun K Sharma, Vittoria Raimondi, Katia Ruggero, Cynthia A Pise-Masison, Ilaria Cavallari, Micol Silic-Benussi, Vincenzo Ciminale, Donna M D'Agostino
Human T-lymphotropic virus 1 (HTLV-1) immortalizes T-cells and is the causative agent of adult T-cell leukemia/lymphoma (ATLL). HTLV-1 replication and transformation are governed by multiple interactions between viral regulatory proteins and host cell factors that remain to be fully elucidated. The present study investigated the impact of HTLV-1 infection on the expression of miR-34a, a microRNA whose expression is downregulated in many types of cancer. Results of RT-PCR assays showed that five out of six HTLV-1-positive cell lines expressed higher levels of miR-34a compared to normal PBMC or purified CD4+ T-cells...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780366/biological-control-of-mango-dieback-disease-caused-by-lasiodiplodia-theobromae-using-streptomycete-and-non-streptomycete-actinobacteria-in-the-united-arab-emirates
#13
Fatima H Kamil, Esam E Saeed, Khaled A El-Tarabily, Synan F AbuQamar
Dieback caused by the fungus Lasiodiplodia theobromae is an important disease on mango plantations in the United Arab Emirates (UAE). In this study, 53 actinobacterial isolates were obtained from mango rhizosphere soil in the UAE, of which 35 (66%) were classified as streptomycetes (SA) and 18 (34%) as non-streptomycetes (NSA). Among these isolates, 19 (12 SA and 7 NSA) showed antagonistic activities against L. theobromae associated with either the production of diffusible antifungal metabolites, extracellular cell-wall-degrading enzymes (CWDEs), or both...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780365/editorial-the-search-for-biological-active-agent-s-from-actinobacteria
#14
EDITORIAL
Learn-Han Lee, Kok-Gan Chan, Jem Stach, Elizabeth M H Wellington, Bey-Hing Goh
No abstract text is available yet for this article.
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780364/antibacterial-efficacy-of-polysaccharide-capped-silver-nanoparticles-is-not-compromised-by-acrab-tolc-efflux-pump
#15
Mitali Mishra, Satish Kumar, Rakesh K Majhi, Luna Goswami, Chandan Goswami, Harapriya Mohapatra
Antibacterial therapy is of paramount importance in treatment of several acute and chronic infectious diseases caused by pathogens. Over the years extensive use and misuse of antimicrobial agents has led to emergence of multidrug resistant (MDR) and extensive drug resistant (XDR) pathogens. This drastic escalation in resistant phenotype has limited the efficacy of available therapeutic options. Thus, the need of the hour is to look for alternative therapeutic approaches to mitigate healthcare concerns caused due to MDR bacterial infections...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780363/microbial-community-analyses-of-the-deteriorated-storeroom-objects-in-the-tianjin-museum-using-culture-independent-and-culture-dependent-approaches
#16
Zijun Liu, Yanhong Zhang, Fengyu Zhang, Cuiting Hu, Genliang Liu, Jiao Pan
In the storeroom C7 of the Tianjin Museum, one wooden desk and two leather luggages dated back to Qing dynasty (1644-1912 AD) presented viable microbial contamination. The aim of the present study was to investigate microbial communities responsible for the biodeterioration of storeroom objects using a combination of culture-independent and culture-dependent methods as well microscopic techniques. Scanning electron microscopy (SEM) revealed that the microflora on three storeroom objects were characterized by a marked presence of Eurotium halophilicum ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780362/characterization-and-genomic-study-of-phage-vb_ecos-b2-infecting-multidrug-resistant-escherichia-coli
#17
Yue Xu, Xinyan Yu, Yu Gu, Xu Huang, Genyan Liu, Xiaoqiu Liu
The potential of bacteriophage as an alternative antibacterial agent has been reconsidered for control of pathogenic bacteria due to the widespread occurrence of multi-drug resistance bacteria. More and more lytic phages have been isolated recently. In the present study, we isolated a lytic phage named vB_EcoS-B2 from waste water. VB_EcoS-B2 has an icosahedral symmetry head and a long tail without a contractile sheath, indicating that it belongs to the family Siphoviridae . The complete genome of vB_EcoS-B2 is composed of a circular double stranded DNA of 44,283 bp in length, with 54...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780361/challenges-and-promises-for-planning-future-clinical-research-into-bacteriophage-therapy-against-pseudomonas-aeruginosa-in-cystic-fibrosis-an-argumentative-review
#18
REVIEW
Martina Rossitto, Ersilia V Fiscarelli, Paola Rosati
Although early aggressive and prolonged treatment with specific antibiotics can extend survival in patients with cystic fibrosis (CF) colonized by opportunistic Pseudomonas aeruginosa (PA), antibiotics fail to eradicate the infecting multidrug-resistant (MDR) PA strains in CF. Century-long research has suggested treating patients with bacteriophages (phages, prokaryotic viruses) naturally hosted by bacteria. Although the only phage types used in therapy, lytic phages, lyse PA aggregated in biofilm matrix by depolymerase degrading enzymes, how they can effectively, safely, and persistently do so in patients with CF is unclear...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29774021/mitotic-spindle-organizing-protein-mzta-mediates-septation-signaling-by-suppressing-the-regulatory-subunit-of-protein-phosphatase-2a-para-in-aspergillus-nidulans
#19
Ping Jiang, Shujun Zheng, Ling Lu
The proper timing and positioning of cytokinesis/septation is crucial for hyphal growth and conidiation in Aspergillus nidulans . The septation initiation network (SIN) components are a conserved spindle pole body (SPB) localized signaling cascade and the terminal kinase complex SidB-MobA, which must localize on the SPB in this pathway to trigger septation/cytokinesis. The regulatory subunit of phosphatase PP2A-ParA has been identified to be a negative regulator capable of inactivating the SIN. However, little is known about how ParA regulates the SIN pathway and whether ParA regulates the septum formation process through affecting the SPB-localized SIN proteins...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29774020/high-diversity-of-myocyanophage-in-various-aquatic-environments-revealed-by-high-throughput-sequencing-of-major-capsid-protein-gene-with-a-new-set-of-primers
#20
Weiguo Hou, Shang Wang, Brandon R Briggs, Gaoyuan Li, Wei Xie, Hailiang Dong
Myocyanophages, a group of viruses infecting cyanobacteria, are abundant and play important roles in elemental cycling. Here we investigated the particle-associated viral communities retained on 0.2 μm filters and in sediment samples (representing ancient cyanophage communities) from four ocean and three lake locations, using high-throughput sequencing and a newly designed primer pair targeting a gene fragment (∼145-bp in length) encoding the cyanophage gp23 major capsid protein (MCP). Diverse viral communities were detected in all samples...
2018: Frontiers in Microbiology
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