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Journal of Molecular Microbiology and Biotechnology

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https://www.readbyqxmd.com/read/29730661/improvement-of-spinosad-production-upon-utilization-of-oils-and-manipulation-of-%C3%AE-oxidation-in-a-high-producing-saccharopolyspora-spinosa-strain
#1
Ying Huang, Xiaolin Zhang, Chen Zhao, Xuhui Zhuang, Lin Zhu, Chao Guo, Yuan Song
Spinosad, a member of polyketide-derived macrolides produced in the actinomycete Saccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285...
May 4, 2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29694975/draft-genome-sequence-of-bacillus-cereus-citvm-11-1-a-strain-exhibiting-interesting-antifungal-activities
#2
Javier Caballero, Cecilia Peralta, Antonella Molla, Eleodoro E Del Valle, Primitivo Caballero, Colin Berry, Verónica Felipe, Pablo Yaryura, Leopoldo Palma
Bacillus cereus is a gram-positive, spore-forming bacterium possessing an important and historical record as a human-pathogenic bacterium. However, several strains of this species exhibit interesting potential to be used as plant growth-promoting rhizobacteria. Here, we report the draft genome sequence of B. cereus strain CITVM-11.1, which consists of 37 contig sequences, accounting for 5,746,486 bp (with a GC content of 34.8%) and 5,752 predicted protein-coding sequences. Several of them could potentially be involved in plant-bacterium interactions and may contribute to the strong antagonistic activity shown by this strain against the charcoal root rot fungus, Macrophomina phaseolina...
April 25, 2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29617701/isolation-and-characterization-of-a-lytic-bacteriophage-vb_pmis-th-and-its-application-in-combination-with-ampicillin-against-planktonic-and-biofilm-forms-of-proteus-mirabilis-isolated-from-urinary-tract-infection
#3
Mahsa Yazdi, Majid Bouzari, Ezzat Allah Ghaemi
Proteus mirabilis is one of the most common causes of urinary tract infection (UTI), particularly in patients undergoing long-term catheterization. Phage vB_PmiS-TH was isolated from wastewater with high lytic activity against P. mirabilis (TH) isolated from UTI. The phage had rapid adsorption, a large burst size (∼260 PFU per infected cell), and high stability at a wide range of temperatures and pH values. As analyzed by transmission electron microscopy, phage vB_PmiS-TH had an icosahedral head of ∼87 × 62 nm with a noncontractile tail about 137 nm in length and 11 nm in width...
April 4, 2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29486469/enhanced-delignification-of-lignocellulosic-biomass-by-recombinant-fungus-phanerochaete-chrysosporium-overexpressing-laccases-and-peroxidases
#4
Nancy Coconi Linares, Francisco Fernández, Achim M Loske, Miguel A Gómez-Lim
Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain...
February 27, 2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29539608/improved-production-of-highly-active-and-pure-human-creatine-kinase-mb
#5
Sureyya Mert Selimoglu, Murat Kasap, Gurler Akpinar, Aynur Karadenizli, Abdul Mounem Wis, Uzay Gormus
Human creatine kinase MB (hCKMB) is one of the most preferred biomarkers used for the diagnosis of acute coronary syndrome due to its high sensitivity and specificity. The increasing need for highly purified and biologically active hCKMB in the field of diagnostics makes its production valuable. Currently, the production of hCKMB is mainly achieved in methylotrophic yeast, Pichia pastoris, because the production in Escherichia coli is challenging and generally yields an inactive enzyme with a low quantity. With the aim of finding the best way for the high-yield production of active hCKMB in E...
2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29510391/cloning-and-characterization-of-a-novel-n-acetylglucosaminidase-atld-from-enterococcus-faecalis
#6
Carlos Eduardo Serrano-Maldonado, Israel García-Cano, Augusto González-Canto, Eliel Ruiz-May, Jose Miguel Elizalde-Contreras, Maricarmen Quirasco
The atlD gene from an Enterococcus faecalis strain isolated from a Mexican artisanal cheese was cloned, sequenced and expressed in Escherichia coli in order to perform a biochemical characterization. A partial amino acid sequence of the heterologous protein was obtained by LC-MS/MS, and it corresponded to a novel peptidoglycan hydrolase designated AtlD. Its molecular mass was 62-75 kDa, as determined by SDS-PAGE, zymography, Western blot, and exclusion chromatography. Electrofocusing rendered an isoelectric point (pI) of 4...
2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29408812/high-expression-of-human-cathepsin-s-by-recombinant-pichia-pastoris-with-cod-skin-as-an-organic-co-nitrogen-source
#7
Guiying Y Li, Man Fu, Mei Qin, Liming M Xue
Human cathepsin S production by recombinant Pichia pastoris using cod skin as the co-nitrogen source was investigated in this study. The addition of carbon sources of glycerol in the fed-batch phase and of methanol in the induction stage was also investigated. A new approach to the highly expression of human cathepsin S was developed using 90 g/L of cod skin (wet weight). After 24 h of the initial fermentation, 4% glycerol (v/v, glycerol/culture) was added once to enhance the cell density (OD600) in the cultivation...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29339632/identification-of-rifampicin-resistance-mutations-in-escherichia-coli-including-an-unusual-deletion-mutation
#8
Eugene Y Wu, Angela K Hilliker
Rifampicin is an effective antibiotic against mycobacterial and other bacterial infections, but resistance readily emerges in laboratory and clinical settings. We screened Escherichia coli for rifampicin resistance and identified numerous mutations to the gene encoding the β-chain of RNA polymerase (rpoB), including an unusual 9-nucleotide deletion mutation. Structural modeling of the deletion mutant indicates locations of potential steric clashes with rifampicin. Sequence conservation in the region near the deletion mutation suggests a similar mutation may also confer resistance during the treatment of tuberculosis...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29258075/contribution-of-pcr-denaturing-gradient-gel-electrophoresis-combined-with-mixed-chromatogram-software-separation-for-complex-urinary-sample-analysis
#9
Iva Kotásková, Barbora Mališová, Hana Obručová, Veronika Holá, Tereza Peroutková, Filip Růžička, Tomáš Freiberger
Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29212086/comparative-analyses-of-transport-proteins-encoded-within-the-genomes-of-bdellovibrio-bacteriovorus-hd100-and-bdellovibrio-exovorus-jss
#10
Fereshteh Heidari Tajabadi, Arturo Medrano-Soto, Masoud Ahmadzadeh, Gholamreza Salehi Jouzani, Milton H Saier
Bdellovibrio, δ-proteobacteria, including B. bacteriovorus (Bba) and B. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and drug efflux systems, and 3 times more lipid transporters...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29197868/the-dual-carboxymethyl-cellulase-and-gelatinase-activities-of-a-newly-isolated-protein-from-brevibacillus-agri-st15c10-confer-reciprocal-regulations-in-substrate-utilization
#11
Smarajit Maiti, Tanmoy Samanta, Sumit Sahoo, Sudipta Roy
A protein showing endoglucanase-peptidase activity was prepared from a newly isolated bacterium (ST15c10). We identified ST15c10 as Brevibacillus agri based on electron-microscopic images and its 16S-rDNA sequence (GenBank accession No. HM446043), which exhibits 98.9% sequence identity to B. agri (KZ17)/B. formosus (DSM-9885T)/B. brevis. The enzyme was purified to homogeneity and gave a single peak during high-performance liquid chromatography on a Seralose 6B-150 gel-matrix/C-18 column. MALDI-TOF mass-spectrometry and bioinformatics studies revealed significant similarity to M42-aminopeptidases/endoglucanases of the CelM family...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29186720/archaeal-and-bacterial-community-structure-in-an-anaerobic-digestion-reactor-lagoon-type-used-for-biogas-production-at-a-pig-farm
#12
Liliana Pampillón-González, Nadia L Ortiz-Cornejo, Marco Luna-Guido, Luc Dendooven, Yendi E Navarro-Noya
Biogas production from animal waste is an economically viable way to reduce environmental pollution and produce valuable products, i.e., methane and a nutrient-rich organic waste product. An anaerobic digestion reactor for biogas production from pig waste was sampled at the entrance, middle (digestion chamber), and exit of a digester, while the bacterial and archaeal community structure was studied by 16S rRNA gene metagenomics. The number of bacterial operational taxonomic units (OTU)-97% was 3-7 times larger than that of archaeal ones...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29183031/novel-pcr-assays-for-the-detection-of-biological-agents-responsible-for-wheat-rust-diseases-puccinia-triticina-and-puccinia-striiformis-f-sp-tritici
#13
Adam Kuzdraliński, Anna Kot, Hubert Szczerba, Agnieszka Ostrowska, Michał Nowak, Marta Muszyńska, Michał Lechowski, Paweł Muzyka
The species Puccinia triticina (Pt) and Puccinia striiformis f. sp. tritici (Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second largest subunits of the RNA polymerase II (rpb2) and β-tubulin 1 (tub1) genes. The specificities of the primers were verified using naturally infected plant materials with visual symptoms of disease...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29166641/agrowastes-as-feedstock-for-the-production-of-endo-%C3%AE-xylanase-from-cohnella-sp-strain-ar92
#14
José H Pisa, Adriana P Manfredi, Nora I Perotti, Héctor G Romero, Javier D Breccia, María Alejandra Martínez
Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29166640/lactic-acid-bacteria-from-andean-grain-amaranth-a-source-of-vitamins-and-functional-value-enzymes
#15
Silvana L Carrizo, Cecilia E Montes de Oca, María Elvira Hébert, Lucila Saavedra, Graciela Vignolo, Jean Guy LeBlanc, Graciela Celestina Rollán
Amaranth is a rediscovered pseudocereal with high nutritional properties. Lactic acid fermentation can increase the functional and nutritional value of foods. The aims of this study were to isolate and evaluate the functionality of lactic acid bacteria (LAB) from amaranth. LAB strains (n = 29) isolated from amaranth sourdough and grains included Lactobacillus (L.) plantarum (n = 8), L. rhamnosus (n = 6), Enterococcus (E.) mundtii (n = 4), E. hermanniensis (n = 3), E. durans (n = 1), Enterococcus sp. (n = 1), Leuconostoc (Lc...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29161696/the-protein-expression-level-of-a-heterogeneous-gene-inserted-in-lipi-1-of-the-listeria-ivanovii-genome-relies-on-its-insertion-orientation
#16
Sijing Liu, Mingjuan Jiang, Lin Su, Tian Tang, Xiang Zhang, Yongyu Li, Qikang Pu, Chenyan Ren, Chuan Wang
Due to its capability to multiply in either phagocytic or nonphagocytic cells, and to subsequently elicit a robust cellular immune response, Listeria ivanovii (LI) is thought to be feasible for developing bacteria-based live attenuated vaccines. We previously generated several recombinant LI strains expressing Mycobacterium tuberculosis antigens. Since the expression level of heterogeneous protein was sometimes very low, we attempted to elucidate the principle of heterogeneous protein expression in such recombinant LI strains...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29145176/the-membrane-attack-complex-perforin-superfamily
#17
Gabriel Moreno-Hagelsieb, Bennett Vitug, Arturo Medrano-Soto, Milton H Saier
The membrane attack complex/perforin (MACPF) superfamily consists of a diverse group of proteins involved in bacterial pathogenesis and sporulation as well as eukaryotic immunity, embryonic development, neural migration and fruiting body formation. The present work shows that the evolutionary relationships between the members of the superfamily, previously suggested by comparison of their tertiary structures, can also be supported by analyses of their primary structures. The superfamily includes the MACPF family (TC 1...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29055951/cell-wall-treated-lactococcus-lactis-increases-the-plasmid-transfer-efficiency-of-internal-ribosome-entry-site-incorporated-lactococcal-bicistronic-vector-into-df1-cells
#18
Nurulfiza Mat Isa, Nur Elina Abdul Mutalib, Noorjahan Banu Alitheen, Adelene Ai-Lian Song, Raha Abdul Rahim
This study demonstrates that cell wall treatment of Lactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid in L. lactis can be transferred into DF1 cells and that both the VP2 and gfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluorescence microscope...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28903115/erap1-and-pde8a-are-downregulated-in-cattle-protected-against-bovine-tuberculosis
#19
Federico Carlos Blanco, Marcelo Abel Soria, Laura Inés Klepp, Fabiana Bigi
Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium bovis that is responsible for significant economic losses worldwide. In spite of its relevance, the limited knowledge about the host immune responses that provide effective protection against the disease has long hampered the development of an effective vaccine. The identification of host proteins with an expression that correlates with protection against bTB would contribute to the understanding of the cattle defence mechanisms against M...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28889121/structure-function-analysis-of-the-periplasmic-escherichia-coli-cyclophilin-ppia-in-relation-to-biofilm-formation
#20
Aggeliki Skagia, Eleni Vezyri, Konstantinos Grados, Anastasia Venieraki, Michael Karpusas, Panagiotis Katinakis, Maria Dimou
The presence of peptidyl-prolyl cis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functions are still not clearly defined. Here, we demonstrate that the ΔppiA deletion strain from Escherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mutant PpiA proteins using a combination of error-prone and site-directed mutagenesis approaches...
2017: Journal of Molecular Microbiology and Biotechnology
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