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Chromosome 18 Studies

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2 papers 0 to 25 followers
By Victoria Miller Trisomy 18 Foundation
Ekaterina V Ilgisonis, Arthur T Kopylov, Victor G Zgoda
Proteome profiling is a type of quantitative analysis that reveals level of protein expression in the sample. Proteome profiling by using selected reaction monitoring is an approach for the Chromosome-centric Human Proteome Project (C-HPP). Here we describe dataset generated in the course of the pilot phase of Russian part of C-HPP, which was focused on human Chr 18 proteins. Proteome profiling was performed using stable isotope-labeled standards (SRM/SIS) for plasma, liver tissue and HepG2 cells. Dataset includes both positive and negative results of protein detection...
September 2016: Data in Brief
Ekaterina V Poverennaya, Arthur T Kopylov, Elena A Ponomarenko, Ekaterina V Ilgisonis, Victor G Zgoda, Olga V Tikhonova, Svetlana E Novikova, Tatyana E Farafonova, Yana Yu Kiseleva, Sergey P Radko, Igor V Vakhrushev, Konstantin N Yarygin, Sergei A Moshkovskii, Olga I Kiseleva, Andrey V Lisitsa, Alexey S Sokolov, Alexander M Mazur, Egor B Prokhortchouk, Konstantin G Skryabin, Elena S Kostrjukova, Alexander V Tyakht, Alexey Yu Gorbachev, Elena N Ilina, Vadim M Govorun, Alexander I Archakov
A gene-centric approach was applied for a large-scale study of expression products of a single chromosome. Transcriptome profiling of liver tissue and HepG2 cell line was independently performed using two RNA-Seq platforms (SOLiD and Illumina) and also by Droplet Digital PCR (ddPCR) and quantitative RT-PCR. Proteome profiling was performed using shotgun LC-MS/MS as well as selected reaction monitoring with stable isotope-labeled standards (SRM/SIS) for liver tissue and HepG2 cells. On the basis of SRM/SIS measurements, protein copy numbers were estimated for the Chromosome 18 (Chr 18) encoded proteins in the selected types of biological material...
August 29, 2016: Journal of Proteome Research
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